2019-04-30 11:00 ~ 2019-04-30 12:00
Venue: IBC R209
Speaker: Dr. Joanne Ho
Current Position: Postdoctoral Fellow, Department of BioSciences, Rice University, Texas, USA
Host: Dr. Yane-Shih Wang
Abstract:
Synthetic biology is centered on the premise of modifying biological systems to change or improve their function. While the genetic code was once thought to be inflexible, in reality it has evolved to maintain a high error rate in some yeast species and has been drastically rewritten in organelles like mitochondria. This fluidity implies that the genetic code is malleable. In this talk, I will discuss the extent of sense codon reassignment achieved using the pyrrolysyl-tRNA synthetase system and selenocysteine incorporation machinery. To facilitate successful application of these systems, gene expression should ideally exhibit user-controlled ON and OFF characteristics. However, in practice the dynamic range of a promoter is often difficult to predict and control. We developed a method that tunes the dynamic range of ligand-inducible promoters, then built multi-input transcriptional logic gates regulated by ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This talk will discuss transcriptional and translational approaches that modify the composition, sensitivity, and circuitry of essential biological processes.-Dr. Joanne Ho
Contact person: Ms. Nancy Liu
Tel: 02-27855696#2061
Email: liukchun@gate.sinica.edu.tw
Institute of Biological Chemistry, Academia Sinica
