研究技術人員
huangkf@gate.sinica.edu.tw +886-2-27855696,4010
黃開發 研究技師
中央研究院生物化學研究所 401室

研究領域與專長

專長

  1. 蛋白質X-光結晶學
  2. 同步輻射X-光繞射數據收集
  3. 結構生物學
  4. 以蛋白質結構為基礎的藥物最佳化
  5. 以結構為基礎的蛋白質分析

研究

  • 以蛋白質結構為基礎的小分子藥物開發、設計及最佳化,以治療神經退化性疾病

 

 

學經歷

  • 2002 – 2006   博士, 生化科學研究所, 國立臺灣大學
  • 1991 – 1993   碩士, 生化科學研究所, 國立臺灣大學
  • 1986 – 1991   學士, 獸醫學系, 國立中興大學
  • 2017 – 迄今   研究技師, 中研院生化所
  • 2016 – 迄今   計畫總經理, 台灣蛋白質計畫
  • 2013 – 2017   研究副技師, 中研院生化所
  • 2011 – 2015   計畫經理, 國家蛋白質結構分析核心設施
  • 2009 – 2012   研究助技師, 中研院生化所
  • 2005 – 2011   計畫經理, 國家蛋白質生產及X-光結晶學核心設施
  • 1993 – 2009   研究助理, 中研院生化所

主要著作

Structural and biological insights into Klebsiella pneumonia surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use. 
Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, Wu WJ, Jan JT, Ho MR, Chou CY, Wang AH, Wu CY, Wang JT, Huang KF, Wu SH 
Journal of Biomedical Science (2022)

A GalNAc/Gal-specific lectin modulates immune responses via toll-like receptor 4 independently of carbohydrate-binding ability. 
Chen ST, Liao JH, Huang KF, Lee IM, Wong WT, Wu SH, Hua KF 
Chemical Communications (2021)

A Unique Carboxylic-Acid Hydrogen-Bond Network (CAHBN) Confers Glutaminyl Cyclase Activity on M28 Family Enzymes. 
Huang KF, Huang JS, Wu ML, Hsieh WL, Hsu KC, Hsu HL, Ko TP, Wang AH 
Journal of Molecular Biology (2021)

Structural basis for fragmenting the exopolysaccharide of Acinetobacter baumannii by bacteriophage ΦAB6 tailspike protein. 
Lee IM, Tu IF, Yang FL, Ko TP, Liao JH, Lin NT, Wu CY, Ren CT, Wang AH, Chang CM, Huang KF, Wu SH 
Scientific Reports (2017)

A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3. 
Liao JH, Chien CT, Wu HY, Huang KF, Wang I, Ho MR, Tu IF, Lee IM, Li W, Shih YL, Wu CY, Lukyanov PA, Hsu ST, Wu SH 
Journal of the American Chemical Society (2016)

Linked Production of Pyroglutamate-Modified Proteins via Self-Cleavage of Fusion Tags with TEV Protease and Autonomous N-Terminal Cyclization with Glutaminyl Cyclase In Vivo. 
Shih YP, Chou CC, Chen YL, Huang KF, Wang AH 
PLoS One (2014)

Structural and functional analyses of a glutaminyl cyclase from Ixodes scapularis reveal metal-independent catalysis and inhibitor binding. 
Huang KF, Hsu HL, Karim S, Wang AH 
Acta Crystallographica Section D : Biological Crystallography (2014)

Structures of Human Golgi-resident Glutaminyl Cyclase and Its Complexes with Inhibitors Reveal a Large Loop Movement upon Inhibitor Binding. 
Huang KF, Liaw SS, Huang WL, Chia CY, Lo YC, Chen YL, Wang AH 
Journal of Biological Chemistry (2011)

A conserved hydrogen-bond network in the catalytic centre of animal glutaminyl cyclases is critical for catalysis. 
Huang KF, Wang YR, Chang EC, Chou TL, Wang AH 
The Biochemical journal (2008)

Crystal structures of human glutaminyl cyclase, an enzyme responsible for protein N-terminal pyroglutamate formation. 
Huang KF, Liu YL, Cheng WJ, Ko TP, Wang AH 
Proceedings of the National Academy of Sciences of the United States of America (2005)

著作列表