Our laboratory has been focusing on (i) mechanistic and structural studies of prenyltransferases catalyzing chain elongation, such as undecaprenyl diphosphate synthase, octaprenyl diphosphate synthase, and geranylgeranyl diphosphate synthase; (ii) antivirus and anti-cancer drug discovery targeting 3C protease from Enterovirus 71, 3C-like proteases from SARS-CoV and MERS-CoV, neuraminidase from avian flu virus, and protein-protein interactions; (iii) multi-functional cellulose/hemicellulose degrading enzymes for biofuel production.
SARS-CoV-2 3CLpro displays faster self-maturation in vitro than SARS-CoV 3CLpro due to faster C-terminal cleavage.
Kuo CJ, Liang PH
FEBS Letters (2022)
Kinetic Characterization and Inhibitor Screening for the Proteases Leading to Identification of Drugs against SARS-CoV-2.
Kuo CJ, Chao TL, Kao HC, Tsai YM, Liu YK, Wang LH, Hsieh MC, Chang SY, Liang PH
Antimicrobial Agents and Chemotherapy (2021)
Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Chang YX, Lin YF, Chen CL, Huang MS, Hsiao M, Liang PH
Cancers (2020)
Targeting β-tubulin/CCT-β complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma.
Liu YJ, Chang YJ, Kuo YT, Liang PH
Carcinogenesis (2020)
Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther.
Yao JY, Teng KH, Liu MC, Wang CS, Liang PH
Molecules (2019)
A flexible loop for mannan recognition and activity enhancement in a bifunctional glycoside hydrolase family 5.
Liang PH, Lin WL, Hsieh HY, Lin TY, Chen CH, Tewary SK, Lee HL, Yuan SF, Yang B, Yao JY, Ho MC
Biochimica et Biophysica Acta (BBA) - General Subjects (2018)
Disrupting CCT-β : β-tubulin selectively kills CCT-β overexpressed cancer cells through MAPKs activation.
Liu YJ, Kumar V, Lin YF, Liang PH
Cell Death & Disease (2017)
Identification and evaluation of potent Middle East respiratory syndrome coronavirus (MERS-CoV) 3CLpro inhibitors.
Kumar V, Shin JS, Shie JJ, Ku KB, Kim C, Go YY, Huang KF, Kim M, Liang PH
Antiviral Research (2017)
Fluorescent farnesyl diphosphate analogue: A probe to validate trans-prenyltransferase inhibitors.
Teng KH, Hsu ET, Chang YH, Lin SW, Liang PH
Biochemistry (2016)
Biochemical characterization and structural analysis of a bifunctional cellulase/xylanase from Clostridium thermocellum.
Yuan SF, Wu TH, Lee HL, Hsieh HY, Lin WL, Yang B, Chang CK, Li Q, Gao J, Huang CH, Ho MC, Guo RT, Liang PH
Journal of Biological Chemistry (2015)