中央研究院 生物化學研究所
中央研究院 生物化學研究所
中央研究院 生物化學研究所
kkhoo@gate.sinica.edu.tw
+886-2-27855696,6030
研發各式串聯式質譜分析技術之應用於蛋白體、蛋白後修飾及醣質體的結構分析、定序與表現差異圖譜的鑑定，致力於提升技術平台的高效率、靈敏度與精確度，以期能更有效的切入探討其功能性。特別針對硫酸化醣質體、蛋白質醣化修飾及其他後修飾的有效鑑定及其於癌細胞與免疫細胞所影嚮的辨識作用、訊息傳遞與調控機制。
High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha.
Chien YC, Wang YS, Sridharan D, Kuo CW, Chien CT, Uchihashi T, Kato K, Angata T, Meng TC, Hsu STD and Khoo KH
JACS Au (2023)
Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants.
Kuo CW, Yang TJ, Chien YC, Yu PY, Hsu SD and Khoo KH
Glycobiology (2022)
Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes.
Tseng HC, Hsiao CT, Yamakawa N, Guérardel Y and Khoo KH
Front Mol Biosci (2021)
A mass spectrometry-based glycotope-centric cellular glycomics is the more fruitful way forward to see the forest for the trees.
Khoo KH
Biochem Soc Trans (2021)
Strategic Applications of Negative-Mode LC-MS/MS Analyses to Expedite Confident Mass Spectrometry-Based Identification of Multiple Glycosylated Peptides.
Kuo CW and Khoo KH
Anal Chem (2020)
Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans.
Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW and Hsu SD
Proc Natl Acad Sci U S A (2020)
Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D2 and adenosine A2A receptors.
Lin CY, Lai HL, Chen HM, Siew JJ, Hsiao CT, Chang HC, Liao KS, Tsai SC, Wu CY, Kitajima K, Sato C, Khoo KH and Chern Y
Transl Psychiatry (2019)
Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns.
Yamakawa N, Vanbeselaere J, Chang LY, Yu SY, Ducrocq L, Harduin-Lepers A, Kurata J, Aoki-Kinoshita KF, Sato C, Khoo KH, Kitajima K and Guerardel Y
Nat Commun (2018)
Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis.
Yu SY, Hsiao CT, Izawa M, Yusa A, Ishida H, Nakamura S, Yagi H, Kannagi R and Khoo KH
J Biol Chem (2018)
Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on NAnoLC-MS2-product Dependent-MS3 ANAlysis of Permethylated Glycans.
Hsiao CT, Wang PW, Chang HC, Chen YY, Wang SH, Chern Y, and Khoo KH*
Mol Cell Proteomics (2017)
Direct Mapping of Additional Modifications on Phosphorylated O-glycans of α-Dystroglycan by Mass Spectrometry Analysis in Conjunction with Knocking Out of Causative Genes for Dystroglycanopathy.
Yagi H, Kuo CW, Obayashi T, Ninagawa S, Khoo KH, and Kato K
Mol Cell Proteomics (2016)
Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans.
Cheng CW, Chou CC, Hsieh HW, Tu Z, Lin CH, Nycholat C, Fukuda M, and Khoo KH
Anal Chem (2015)
