中央研究院 生物化學研究所

研究領域與專長

1. 維護本所生物物理公用儀器室(BIL)各項設施並提供使用者相關儀器的操作課程及技術支援。
2. 量測生物分子間結合力與動力結合速率以及利用熱動力學方式研究交互作用力。
3. 應用生物物理學技術探討蛋白質折疊、穩定性、構型、聚集與聚合等特性。

學經歷

• 2003 – 2008   博士, 生物資訊與結構生物學研究所, 國立清華大學

• 2021 – 迄今   研究副技師, 中央研究院生物化學研究所
• 2016 – 2021   研究助技師, 中央研究院生物化學研究所
• 2012 – 2016   生物物理公用儀器室經理, 中央研究院生物化學研究所
• 2011 – 2012   博士後研究員, 美國德州大學達拉斯西南醫學中心
• 2008 – 2011   博士後研究員, 中央研究院生物醫學研究所

主要著作

A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3. 
Liao JH, Chien CT, Wu HY, Huang KF, Wang I, Ho MR, Tu IF, Lee IM, Li W, Shih YL, Wu CY, Lukyanov PA, Hsu ST, Wu SH 
Journal of the American Chemical Society (2016)

Structural Basis for the Magnesium-Dependent Activation and Hexamerization of the Lon AAA+ Protease. 
Su SC, Lin CC, Tai HC, Chang MY, Ho MR, Babu CS, Liao JH, Wu SH, Chang YC, Lim C, Chang CI 
Structure (2016)

Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses. 
Weng JH, Hsieh YC, Huang CC, Wei TY, Lim LH, Chen YH, Ho MR, Wang I, Huang KF, Chen CJ, Tsai MD 
Biochemistry (2015)

Substrate specificity and plasticity of FERM-containing protein tyrosine phosphatases. 
Chen KE, Li MY, Chou CC, Ho MR, Chen GC, Meng TC, Wang AH 
Structure (2015)

Structure of yeast Ape1 and its role in autophagic vesicle formation. 
Su MY, Peng WH, Ho MR, Su SC, Chang YC, Chen GC, Chang CI 
Autophagy (2015)

Solution structure and tandem DNA recognition of the C-terminal effector domain of PmrA from Klebsiella pneumoniae. 
Lou YC, Wang I, Rajasekaran M, Kao YF, Ho MR, Hsu ST, Chou SH, Wu SH, Chen C 
Nucleic acids research (2014)

Reciprocal allosteric regulation of p38gamma and PTPN3 involves a PDZ domain-modulated complex formation. 
Chen KE, Lin SY, Wu MJ, Ho MR, Santhanam A, Chou CC, Meng TC, Wang AH 
Science signaling (2014)

Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. 
Chang TH, Chang SJ, Hsieh FL, Ko TP, Lin CT, Ho MR, Wang I, Hsu ST, Guo RT, Chang W, Wang AH 
PLoS pathogens (2013)

Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites. 
Ho MR, Lou YC, Wei SY, Luo SC, Lin WC, Lyu PC, Chen C 
Journal of molecular biology (2010)

Human pancreatitis-associated protein forms fibrillar aggregates with a native-like conformation. 
Ho MR, Lou YC, Lin WC, Lyu PC, Huang WN, Chen C 
The Journal of biological chemistry (2006)

著作列表

1. (Ho MR), Wu YM, Lu YC, Ko TP, Wu KP*  (2023-03 accepted)  Journal of Structural Biology  "Cryo-EM reveals the structure and dynamics of a 723-residue malate synthase G"
   
2. Chen EH, Lin KM, Sang JC, (Ho MR), Lee CH, Shih O, Su CJ, Yeh YQ, Jeng US, Chen RP*  (2022-08)  IUBMB life  74(8), 780-793  "Condition-dependent structural collapse in the intrinsically disordered N-terminal domain of prion protein."
   
3. Huang PC, Chen SK, Chiang WH, (Ho MR), Wu KP*  (2022-05)  Protein Science  31(5), e4304  "Structural basis for the helical filament formation of Escherichia coli glutamine synthetase."
   
4. Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, Wu WJ, Jan JT, (Ho MR), Chou CY, Wang AH, Wu CY, Wang JT, Huang KF*, Wu SH*  (2022-02)  Journal of Biomedical Science  29(1), 9  "Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use."
   
5. Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, (Ho MR), Chen WY, Lin HT, Wu HC, Hsu STD*  (2021-09)  NATURE STRUCTURAL & MOLECULAR BIOLOGY  28(9), 731-739  "Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function"
   
6. Huang CT, Lai YC, Chen SY, (Ho MR), Chiang YW, Hsu STD*  (2021-06)  Magnetic Resonance  2(1), 375-386  "Structural polymorphism and substrate promiscuity of a ribosome-associated molecular chaperone"
   
7. Chiu YH, Ko KT, Yang TJ, Wu KP, (Ho MR), Draczkowski P*, Hsu STD*  (2021-04)  Biochemistry  60(14), 1075-1079  "Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein."
   
8. Wu AY, Sung YC, Chen YJ, Chou ST, Guo V, Chien JC, Ko JJ, Yang AL, Huang HC, Chuang JC, Wu S, (Ho MR), Ericsson M, Lin WW, Cheung CHY, Juan HF, Ueda K, Chen Y, Lai CP  (2020-10)  ADVANCED SCIENCE  7(19), 2001467  "Multiresolution Imaging Using Bioluminescence Resonance Energy Transfer Identifies Distinct Biodistribution Profiles of Extracellular Vesicles and Exomeres with Redirected Tropism"
   
9. Chueh CK, Som N, Ke LC, (Ho MR), Reddy M, Chang CI  (2019-08)  mBio  10(4), e01129-19  "Structural basis for the differential regulatory roles of the PDZ domain in C-terminal processing proteases."
   
10. Lee CH, Shih YP, (Ho MR), Wang AH  (2018-12)  Nucleic acids research  46(22), 11806-11821  "The C-terminal D/E-rich domain of MBD3 is a putative Z-DNA mimic that competes for Zalpha DNA-binding activity."
    
11. de Jong APH, Roggero CM, (Ho MR), Wong MY, Brautigam CA, Rizo J, Kaeser PS  (2018-04)  Neuron  98(2), 335-349.e7  "RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes."
    
12. Liao JH, Tsai CH, Patel SG, Yang JT, Tu IF, Lo Cicero M, Lipka-Lloyd M, Wu WL, Shen WJ, (Ho MR), Chou CC, Sharma GR, Okanishi H, Luk LYP, Tsai YH, Wu SH  (2017-11)  Frontiers in molecular biosciences  4, 77  "Acetylome of Acinetobacter baumannii SK17 Reveals a Highly-Conserved Modification of Histone-Like Protein HU."
    
13. Su MY, Som N, Wu CY, Su SC, Kuo YT, Ke LC, (Ho MR), Tzeng SR, Teng CH, Mengin-Lecreulx D, Reddy M, Chang CI  (2017-11)  Nature Communications  8, 1516  "Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc."
    
14. Chien CTH, (Ho MR), Lin CH, Hsu STD*  (2017-08)  MOLECULES  22(8), 1357  "Lactose Binding Induces Opposing Dynamics Changes in Human Galectins Revealed by NMR-Based Hydrogen-Deuterium Exchange."
    
15. Lee YC, Chang CY, Chen SY, Pan YR, (Ho MR), Hsu SD  (2017-03)  Scientific reports  7, 45174  "Entropic stabilization of a deubiquitinase provides conformational plasticity and slow unfolding kinetics beneficial for functioning on the proteasome."
    
16. Su SC, Lin CC, Tai HC, Chang MY, (Ho MR), Babu CS, Liao JH, Wu SH, Chang YC, Lim C, Chang CI  (2016-05)  Structure  24(5), 676-686  "Structural Basis for the Magnesium-Dependent Activation and Hexamerization of the Lon AAA+ Protease."
    
17. Yang Y, Ko TP, Chen CC, Huang G, Zheng Y, Liu W, Wang I, (Ho MR), Hsu ST, O'Dowd B, Huff HC, Huang CH, Docampo R, Oldfield E, Guo RT  (2016-05)  ACS chemical biology  11(5),1362-1371  "Structures of Trypanosome Vacuolar Soluble Pyrophosphatases: Antiparasitic Drug Targets."
    
18. Liao JH, Chien CT, Wu HY, Huang KF, Wang I, (Ho MR), Tu IF, Lee IM, Li W, Shih YL, Wu CY, Lukyanov PA, Hsu ST, Wu SH  (2016-04)  Journal of the American Chemical Society  138(14), 4787-4795  "A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3."
    
19. Su MY, Peng WH, (Ho MR), Su SC, Chang YC, Chen GC, Chang CI  (2015)  Autophagy  11(9), 1580-1593  "Structure of yeast Ape1 and its role in autophagic vesicle formation."
    
20. Chen KE, Li MY, Chou CC, (Ho MR), Chen GC, Meng TC, Wang AH  (2015)  Structure  23(4), 653-664  "Substrate specificity and plasticity of FERM-containing protein tyrosine phosphatases."
    
21. Weng JH, Hsieh YC, Huang CC, Wei TY, Lim LH, Chen YH, (Ho MR), Wang I, Huang KF, Chen CJ, Tsai MD  (2015)  Biochemistry  54(40), 6219-6229  "Uncovering the Mechanism of Forkhead-Associated Domain-Mediated TIFA Oligomerization That Plays a Central Role in Immune Responses."
    
22. Chen KE, Lin SY, Wu MJ, (Ho MR), Santhanam A, Chou CC, Meng TC, Wang AH  (2014)  Science signaling  7(347), ra98  "Reciprocal allosteric regulation of p38gamma and PTPN3 involves a PDZ domain-modulated complex formation."
    
23. Lou YC, Wang I, Rajasekaran M, Kao YF, (Ho MR), Hsu ST, Chou SH, Wu SH, Chen C  (2014)  Nucleic acids research  42(6), 4080-4093  "Solution structure and tandem DNA recognition of the C-terminal effector domain of PmrA from Klebsiella pneumoniae."
    
24. Chang TH, Chang SJ, Hsieh FL, Ko TP, Lin CT, (Ho MR), Wang I, Hsu ST, Guo RT, Chang W, Wang AH  (2013)  PLoS pathogens  9(8), e1003563  "Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein."
    
25. (Ho MR), Chen C  (2012)  Biomolecular NMR assignments  6(2), 135-137  "1H, 13C, and 15N resonance assignments for human regenerating family Ialpha protein."
    
26. Wei SY, Lou YC, Tsai JY, (Ho MR), Chou CC, Rajasekaran M, Hsu HM, Tai JH, Hsiao CD, Chen C  (2012)  Nucleic acids research  40(1), 449-460  "Structure of the Trichomonas vaginalis Myb3 DNA-binding domain bound to a promoter sequence reveals a unique C-terminal beta-hairpin conformation."
    
27. (Ho MR), Lou YC, Wei SY, Luo SC, Lin WC, Lyu PC, Chen C  (2010)  Journal of molecular biology  402(4), 682-695  "Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites."
    
28. (Ho MR), Lou YC, Lin WC, Lyu PC, Huang WN, Chen C  (2006)  The Journal of biological chemistry  281(44), 33566-33576  "Human pancreatitis-associated protein forms fibrillar aggregates with a native-like conformation."
    
29. (Ho MR), Lou YC, Lin WC, Lyu PC, Chen C  (2004)  Journal of biomolecular NMR  30(3), 381-382  "(1)H, (13)C, and (15)N resonance assignments and secondary structure of human pancreatitis-associated protein (hPAP)."