中央研究院 生物化學研究所

研究領域與專長

Manager of synthesis core facility (SCF), supporting users as follows:

• Various synthesized biomolecules as research stuffs, for example:
  Functional peptides;
  Enzyme/lectin inhibitors;
  Oligonucleotides and their bioconjugates;
  Glycan related to viral/bacterial infections;
  Tumor-associated carbohydrate antigens (TACAs);
  Probes of metabolites in natural products biosynthesis;
  Tools to study targets of interest in chemistry and biology;
  Other tailor-made synthesized molecules.
• High-end and well-maintained instruments including:
  HPLC (Waters e2695, on-line reservation);
  HPLC (Schimadzu Prominence-I LC-2030C 3D Plus, on-line reservation);
  Polarimeter (JASCO P-2000, Na light source (589 nm) with temperature-controlled chip module, on-line reservation).

Organic synthesis:

Synthesis of natural product or bacterial metabolites; strategy development to label intermediate of interest; design and synthesis of wash-free fluorescent probe; synthesis and purification of oligonucleotide-peptide conjugates (OPC) with varied linkers.

Carbohydrate chemistry:

Synthesis and characterization of well-patterned sulfated sugars; Immobilization and NMR characterization of well-defined sulfated glycans on polyacrylic acid (PAA); Structure-guided synthesis of galectin inhibitors; Incorporation of BODIPY to thiodigalactoside as the fluorescent polarization (FP) probe for galectin binding assay: a better probe with bio-compatible, lifetime, stability and could be applied for cell-based experiments.

Laboratory design:

This is more like personal interest and I have managed labs for two PIs and involved in design and renovation of three labs.

學經歷

• 2002 – 2006   博士, 化學系所, 國立台灣師範大學
• 1997 – 2001   學士, 化學系, 國立台灣師範大學

• 2020 – 迄今   研究助技師, 合成核心設施, 中研院生化所
• 2016 – 2020   專案經理,中研院生化所
• 2016 – 2016   訪問博士後研究員, Centennial Centre of Interdisciplinary Science (CCIS), 加拿大雅伯達大學
• 2007 – 2016   博士後研究員, 中研院生化所

主要著作

Selection of Galectin-3 Ligands Derived from Genetically Encorded Glycopeptide Libraries. 
Vinals, D. F.; Kitov, P. I.; Tu, Z. ; Zou, C.; Cairo, C. W.; Lin, C.-H. and Derda, R.* 
Peptide Sci. (2019)

Dissecting the Structure–Activity Relationship of Galectin–Ligand Interactions. 
Chan, Y.-C. ^†; Lin, H.-Y. ^†; Tu, Z. ^†; Kuo, Y.-H.; Hsu, Danny S.-T. and Lin, C.-H.* 
(^† Authors contributed equally to this work) 
Int. J. Mol. Sci. (2018)

Dual Thio-digalactoside-binding Modes of Human Galectins as the Structural Basis for the Design of Potent and Selective Inhibitors. 
Lin, H.-Y.; Hsieh, T.-Y.; Tu, Z.; Chien, C.-T.; Huang, B.-S.; Wu, S.-C. and Lin, C.-H.* 
Scientific Reports (2016)

Expeditious Synthesis of Orthogonally Protected Saccharides through Consecutive Protection/Glycosylation Steps. 
Tu, Z.; Liu, P.-K.; Wu, M.-C. and Lin, C.-H.* 
Israel J. Chem. (2015)

Development of Fucosyltransferase and Fucosidase Inhibitors. 
Tu, Z.; Lin, Y.-N. and Lin, C.-H.* 
Chem. Soc. Rev. (2013)

Synthesis and Characterization of Sulfated Galβ-1,3/4-GlcNAc Disaccharides via Consecutive Protection/Glycosylation Steps. 
Tu, Z.; Hsieh, H.-W.; Tsai, C.-M.; Hsu, C.-W.; Wang, S.-G.; Wu, K.-J.; Lin, K.-I* and Lin, C.-H.* 
Chem. Asian J. (2013)

Rapid Synthesis of Oligomannosides with Orthogonally Protected Monosaccharides. 
Chang, S.-M.; Tu, Z.; Jan, H.-M.; Pan, C.-F. and Lin, C.-H.* 
Chem. Commum. (2013)

Straightforward and Highly Efficient Catalyst-Free Regioselective Reaction of Thiol to β-Nitrostyrene: A Concise Synthesis of Vinyl Sulfide and Nitro Sulfide. 
Chu, C.-M. ^†; Tu, Z.^†; Wu, P.; Wang, C.-C.; Liu, J.-T.; Kuo, C.-W.; Shin, Y.-H. and Yao, C.-F.* 
(^† Authors contributed equally to this work) 
Tetrahedron (2009)

An Efficient Method for the Synthesis of α-Arylated Nitroalkanes and α-Arylated Hydroximoyl Chlorides Mediated by AlCl₃. 
Tu, Z.; Raju, B. R.; Liou, T.-R.; Kavala, V.; Kuo, C.-W.; Jang, Y.; Shih, Y.-H.; Wang, C.-C. and Yao, C.-F.* 
Tetrahedron (2009)

An Investigation of the Reaction of 2-Aminobenzaldehyde Derivatives with Conjugated Nitro-olefins: An Easy and Efficient Synthesis of 3-Nitro-1,2-dihydroquinolines and 3-Nitroquinolines. 
Yan, M.-C.; Tu, Z.; Lin, C.; Ko, S.; Hsu, J. and Yao, C.-F.* 
J. Org. Chem. (2004)

著作列表