中央研究院 生物化學研究所
中央研究院 生物化學研究所
中央研究院 生物化學研究所
rhchen@gate.sinica.edu.tw
+886-2-27855696,6020
1. 調節蛋白質降解和外泌體分泌以操控癌細胞信號傳遞和腫瘤免疫微環境
我們研究細胞如何確保溶酶體降解和外泌體分泌之間的平衡，蛋白質泛素化如何影響這種平衡，以及癌細胞如何擾亂這種平衡以增強腫瘤惡性表型和免疫抑制性腫瘤微環境。這些調控機制也將被用於設計新的抗癌策略。
2. 靶向致癌蛋白的蛋白酶體和溶酶體降解用於抗癌治療
降解”已成為抗癌策略的新概念，與傳統的“抑制”策略相比具有多項優勢。我們研究幾種主要致癌蛋白的蛋白酶體和溶酶體降解途徑及策略。
3. 生物分子凝聚物對癌症代謝的調控
生物分子凝聚物 (biomolecular condensates)是通過液-液相分離組裝的細胞內無膜隔室，由蛋白質和核酸之間的弱相互作用引起。生物分子凝聚物的失調與神經退化性疾病和癌症等疾病相關。我們研究影響癌細胞代謝的生物分子凝聚物的組裝、拆卸機制和內部組成。
PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly.
Chou MC, Wang YH, Chen FY, Kung CY, Wu KP, Kuo JC, Chan SJ, Cheng ML, Lin CY, Chou YC, Ho MC, Firestine S, Huang Jr, Chen RH
Molecular Cell (2023)
TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation.
Chen YH, Chen HH, Wang WJ, Chen HY, Huang WS, Kao CH, Lee SR, Yeat NY, Yan RL, Chan SJ, Wu KP, Chen RH
Nature Communications (2023)
VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism.
Chen YH, Huang TY, Lin YT, Lin SY, Li WH, Hsiao HJ, Yan RL, Tang HW, Shen ZQ, Chen GC, Wu KP, Tsai TF, Chen RH
Nature Communications (2021)
Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization.
Chiang SY, Wu HC, Lin SY, Chen HY, Wang CF, Yeh NH, Shih JH, Huang YS, Kuo HC, Chou SJ, Chen RH
Science Advances (2021)
BIK ubiquitination by the E3 ligase Cul5-ASB11 determines cell fate during cellular stress. (selected in Special Collection of Articles on Cancer Cell Biology)
Chen FY, Huang MY, Lin YM, Ho CH, Lin SY, Chen HY, Hung MC, Chen RH
J Cell Biol (2019)
Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment. (Selected as a highlight in JCI This Month)
Wang YT, Chen J, Chang CW, Jen J, Huang TY, Chen CM, Shen R, Liang SY, Cheng IC, Yang SC, Lai WW, Cheng KH, Hsieh TS, Lai MZ, Cheng HC, Wang YC, Chen RH
J Clin Invest (2017)
Cul3-KLHL20 Ubiquitin Ligase Governs the Turnover of ULK1 and VPS34 Complexes to Control Autophagy Termination. (Highlighted in Editor’s Corner and Selected for a ‘Punctum’ contribution in Autophagy)
Liu CC, Lin YC, Chen YH, Chen CM, Pang LY, Chen HA, Wu PR, Lin MY, Jiang ST, Tai TF, Chen RH
Mol Cell (2016)
K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. (selected as a Mol Cell Preview and a Research Highlight in Nature Rev Mol Cell Biol)
Yuan WC, Lee YR, Lin SY, Chang LY, Tan YP, Hung CC, Kuo JC, Liu CH, Lin MY, Xu M, Chen ZJ, Chen RH
Mol Cell (2014)
USP11 regulates PML stability to control Notch-induced malignancy in brain tumours.
Wu HC, Lin YC, Liu CH, Chung HC, Wang YT, Lin YW, Ma HI, Tu PH, Lawler SE, Chen RH
Nature Commun (2014)
A Cullin3-KLHL20 Ubiquitin Ligase-Dependent Pathway Targets PML to Potentiate HIF-1 Signaling and Prostate Cancer Progression. (recommended by Faculty of 1000 Biology as F1000 factor 9.0 “MUST READ” grade)
Yuan WC, Lee YR, Huang SF, Lin YM, Chen TY, Chung HC, Tsai CH, Chen HY, Chiang CT, Lai CK, Lu LT, Chen CH, Gu DL, Pu YS, Jou YS, Lu KP, Hsiao PW, Shih HM, Chen RH
Cancer cell (2011)
