中央研究院 生物化學研究所

研究領域與專長

• Manager of Biophysics Core Facility (BCF), Department of Academic Affairs and Instrument Service,
  supporting BCF users of following technologies:
  1. Analytical ultracentrifugation (AUC)
  2. Bio-layer Interferometry (BLI)
  3. Capillary isoelectric focusing technology (cIEF)
  4. Circular dichroism (CD )
  5. Dynamic light scattering (DLS)
  6. Microcalorimetry, ITC & DSC
  7. MicroScale Thermophoresis (MST)
  8. Surface plasmon resonance technology (SPR)
• Protein Chemistry:

Protein over-expression using E. coli or yeast system, various purification techniques (precipitation, gel filtration, ion-exchange, hydrophobic interaction, reverse phase HPLC, affinity chromatography), electrophoresis, steady-state enzyme kinetics, site directed mutagenesis

• Computational Chemistry:

Modeling protein-protein and protein-ligand interactions, Homology modeling, and electrostatic calculations by Discovery Studio, CHARMM or pKa suite

• Web Design:

This is not much a “research interest”, only my interest.
Macromedia Dreamweaver, Fireworks, Flash, Adobe Photoshop, Illustrator, workstation administration, PHP and MySQL database management

學經歷

• 1993 – 1999   博士, 化學, Case Western Reserve University
• 1993 – 1996   碩士, 化學, Case Western Reserve University
• 1986 – 1990   學士, 化學, National Taiwan Normal University

• 2009 – 迄今   Associate Research Specialist, Institute of Biological Chemistry/ Scientific Instrument Center
• 2008 – 2009   Assistant Research Specialist, Inst. of Biological Chemistry / Scientific Instrument Center
• 2005 – 2008   委員, 中研院儀器中心, 核磁共振小組
• 2004 – 2008   Assistant Research Specialist, Inst. of Biological Chemistry, Academia Sinica
• 2001 – 2004   Postdoctoral Fellow, Chemistry, Academia Sinica
• 1999 – 2001   博士後研究員, Biochemistry and Biophysics, Texas A&M University
• 1999 – 1999   Postdoctoral Research Associate, Medicinal Chemistry and Molecular Pharmacology, Purdue University
• 1994 – 1999   Research Assistant, Case Western Reserve University
• 1993 – 1994   Teaching Assistant, Case Western Reserve University
• 1990 – 1992   Chemistry and Physics Teacher, Chung-Shan Junior High

主要著作

The calcium-chelating capability of tetrahydrofuranic moieties modulates the cytotoxicity of annonaceous acetogenins. 
Liaw CC, Liao WY, Chen CS, Jao SC, Wu YC, Shen CN, Wu SH 
Angewandte Chemie-International Edition (2011)

Polyfluorinated bipyridine cisplatins manipulate cytotoxicity through the induction of S-G(2)/M arrest and partial intercalation mechanism. 
Chang TT, More SV, Lu N, Jhuo JW, Chen YC, Jao SC, Li WS 
Bioorganic & medicinal chemistry (2011)

Inhibition of Glutathione S-Transferase M1 by New Gabosine Analogues Is Essential for Overcoming Cisplatin Resistance in Lung Cancer Cells. 
Wang CH, Wu HT, Cheng HM, Yen TJ, Lu IH, Chang HC, Jao SC, Shing TK, Li WS 
Journal of medicinal chemistry (2011)

Overcoming the drug resistance in breast cancer cells by rational design of efficient glutathione S-transferase inhibitors. 
Li WS, Lam WS, Liu KC, Wang CH, Chang HC, Jen YC, Hsu YT, Shivatare SS, Jao SC 
Organic letters (2010)

Synthesis and structure-activity relationships of novel furazan-3,4-diamide analogs as potent anti-cancer agents. 
Li WS, More SV, Wang CH, Jen YC, Yao CF, Wang TF, Hung CC, Jao SC 
Bioorganic & medicinal chemistry letters (2010)

Evaluation of organophosphorus chemicals-degrading enzymes: a comparison of Escherichia coli and human cytosolic aminopeptidase P. 
Y.-T. Hsu, C.-Y. Su, H.-C. Du, S.-C. Jao & W.-S. Li 
Chemistry & biodiversity (2008)

Enzymatic and Nonenzymatic Synthesis of Glutathione Conjugates: Application to the Understanding of Parasite’s Defense System and Alternative to the Discovery of Potent Glutathione S-Transferase Inhibitors. 
W.-J. Lo, Y.-C. Chiou, Y.-T. Hsu, W. S. Lam, M.-Y. Chang, S.-C. Jao & W.-S. Li 
Bioconjugate Chemistry (2007)

Design of potent inhibitors for Schistosoma japonica glutathione S-transferase. 
S.-C. Jao, J. Chen, K. Yang & W.-S. Li 
Bioorganic & Medicinal Chemistry (2006)

Computational and mutational analysis of human glutaredoxin (thioltransferase): probing the molecular basis of the low pKa of cysteine 22 and its role in catalysis. 
S.-C. Jao, S. M. English Ospina, A. J. Berdis, D. W. Starke, C. B. Post & J. J. Mieyal 
Biochemistry (2006)

Aminopeptidase p mediated detoxification of organophosphonate analogues of sarin: mechanistic and stereochemical study at the phosphorus atom of the substrate. 
L.-F. Huang, B. Su, S.-C. Jao, K.-T. Liu & W.-S. Li 
Chembiochem : a European journal of chemical biology (2006)

著作列表

1. Wang WM, Yu CH, Chang JY, Chen TH, Chen YC, Sun YT, Wang SH, (Jao SC), Cheng RP*  (2021-02)  Org Biomol Chem.  19(8), 1860-1866  "Insertion of Pro-Hyp-Gly provides 2 kcal mol -1 stability but attenuates the specific assembly of ABC heterotrimeric collagen triple helices."
   
2. Chang HC, Kung CC, Chang TT, (Jao SC), Hsu YT, Li WS  (2018-01)  PloS one  13(1), e0190816  "Investigation of the proton relay system operative in human cystosolic aminopeptidase P."
   
3. Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, Bakhtina MM, Becker DF, Bedwell GJ, Bekdemir A, Besong TM, Birck C, Brautigam CA, Brennerman W, Byron O, Bzowska A, Chaires JB, Chaton CT, Colfen H, Connaghan KD, Crowley KA, Curth U, Daviter T, Dean WL, Diez AI, Ebel C, Eckert DM, Eisele LE, Eisenstein E, England P, Escalante C, Fagan JA, Fairman R, Finn RM, Fischle W, de la Torre JG, Gor J, Gustafsson H, Hall D, Harding SE, Cifre JG, Herr AB, Howell EE, Isaac RS, (Jao SC), Jose D, Kim SJ, Kokona B, Kornblatt JA, Kosek D, Krayukhina E, Krzizike D, Kusznir EA, Kwon H, Larson A, Laue TM, Le Roy A, Leech AP, Lilie H, Luger K, Luque-Ortega JR, Ma J, May CA, Maynard EL, Modrak-Wojcik A, Mok YF, Mucke N, Nagel-Steger L, Narlikar GJ, Noda M, Nourse A, Obsil T, Park CK, Park JK, Pawelek PD, Perdue EE, Perkins SJ, Perugini MA, Peterson CL, Peverelli MG, Piszczek G, Prag G, Prevelige PE, Raynal BD, Rezabkova L, Richter K, Ringel AE, Rosenberg R, Rowe AJ, Rufer AC, Scott DJ, Seravalli JG, Solovyova AS, Song R, Staunton D, Stoddard C, Stott K, Strauss HM, Streicher WW, Sumida JP, Swygert SG, Szczepanowski RH, Tessmer I, Toth RT 4th, Tripathy A, Uchiyama S, Uebel SF, Unzai S, Gruber AV, von Hippel PH, Wandrey C, Wang SH, Weitzel SE, Wielgus-Kutrowska B, Wolberger C, Wolff M, Wright E, Wu YS, Wubben JM, Schuck P  (2015-05)  PloS one  10(5), e0126420  "A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation."
   
4. More SV, Chang TT, Chiao YP, (Jao SC), Lu CK, Li WS  (2013)  European journal of medicinal chemistry  64, 169-178  "Glycosylation enhances the anti-migratory activities of isomalyngamide A analogs."
   
5. Li WS, Wang CH, Ko S, Chang TT, Jen YC, Yao CF, More SV, Jao SC  (2012)  Journal of medicinal chemistry  55(4), 1583-1592  "Synthesis and evaluation of the cytotoxicities of tetraindoles: observation that the 5-hydroxy tetraindole (SK228) induces G(2) arrest and apoptosis in human breast cancer cells."
   
6. Wang CH, Wu HT, Cheng HM, Yen TJ, Lu IH, Chang HC, (Jao SC), Shing TK, Li WS  (2011-12)  Journal of medicinal chemistry  54(24), 8574-8581  "Inhibition of Glutathione S-Transferase M1 by New Gabosine Analogues Is Essential for Overcoming Cisplatin Resistance in Lung Cancer Cells."
   
7. Chang TT, More SV, Lu N, Jhuo JW, Chen YC, (Jao SC), Li WS  (2011)  Bioorganic & medicinal chemistry  19(16), 4887-4894  "Polyfluorinated bipyridine cisplatins manipulate cytotoxicity through the induction of S-G(2)/M arrest and partial intercalation mechanism."
   
8. Liaw CC, Liao WY, Chen CS, (Jao SC), Wu YC, Shen CN, Wu SH  (2011)  Angewandte Chemie-International Edition  50(34), 7885-7891  "The calcium-chelating capability of tetrahydrofuranic moieties modulates the cytotoxicity of annonaceous acetogenins."
   
9. Li WS, More SV, Wang CH, Jen YC, Yao CF, Wang TF, Hung CC, (Jao SC)  (2010-02)  Bioorganic & medicinal chemistry letters  20(3), 1148-52  "Synthesis and structure-activity relationships of novel furazan-3,4-diamide analogs as potent anti-cancer agents."
   
10. Li WS, Lam WS, Liu KC, Wang CH, Chang HC, Jen YC, Hsu YT, Shivatare SS, (Jao SC)  (2010-01)  Organic letters  12(1), 20-3  "Overcoming the drug resistance in breast cancer cells by rational design of efficient glutathione S-transferase inhibitors."
    
11. Y.-T. Hsu, C.-Y. Su, H.-C. Du, S.-C. Jao & W.-S. Li  (2008)  Chemistry & biodiversity  5(7), 1401-11  "Evaluation of organophosphorus chemicals-degrading enzymes: a comparison of Escherichia coli and human cytosolic aminopeptidase P."
    
12. W.-J. Lo, Y.-C. Chiou, Y.-T. Hsu, W. S. Lam, M.-Y. Chang, S.-C. Jao & W.-S. Li  (2007)  Bioconjugate Chemistry  18, 109-120  "Enzymatic and Nonenzymatic Synthesis of Glutathione Conjugates: Application to the Understanding of Parasite's Defense System and Alternative to the Discovery of Potent Glutathione S-Transferase Inhibitors."
    
13. L.-F. Huang, B. Su, S.-C. Jao, K.-T. Liu & W.-S. Li  (2006)  Chembiochem : a European journal of chemical biology  7(3), 506-14  "Aminopeptidase p mediated detoxification of organophosphonate analogues of sarin: mechanistic and stereochemical study at the phosphorus atom of the substrate."
    
14. S.-C. Jao, S. M. English Ospina, A. J. Berdis, D. W. Starke, C. B. Post & J. J. Mieyal  (2006)  Biochemistry  45(15), 4785-96  "Computational and mutational analysis of human glutaredoxin (thioltransferase): probing the molecular basis of the low pKa of cysteine 22 and its role in catalysis."
    
15. S.-C. Jao, J. Chen, K. Yang & W.-S. Li  (2006)  Bioorganic & Medicinal Chemistry  14, 304-318  "Design of potent inhibitors for Schistosoma japonica glutathione S-transferase."
    
16. S.-C. Jao, L.-F. Huang, Y.-S. Tao & W.-S. Li  (2006)  Bioorganic & medicinal chemistry  14(2), 304-18  "Design of potent inhibitors for Schistosoma japonica glutathione S-transferase."
    
17. U. Srinivasan, A. Bala, S.-C. Jao, D. W. Starke, T. W. Jordan & J. J. Mieyal  (2006)  Biochemistry  45(29), 8978-87  "Selective inactivation of glutaredoxin by sporidesmin and other epidithiopiperazinediones."
    
18. L. Zhang, M. Derider, M.A. McCornack, S.-C. Jao, N. Isern, T. Ness, R. Moyer & P.J. LiWang  (2006)  Proceedings of the National Academy of Sciences of the United States of America  103(38), 13985-90  "Solution structure of the complex between poxvirus-encoded CC chemokine inhibitor vCCI and human MIP-1beta."
    
19. Y.-L. Yang, F.-L. Yang, S.-C. Jao, M.-Y. Chen, S.-S. Tsay, W. Zou & S.-H.Wu   (2006)  Journal of lipid research  47(8), 1823-32  "Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus."
    
20. S.-C. Jao, L.-F. Huang, S.-M. Hwang & W.-S. Li  (2006)  Biochemistry  45(6), 1547-53  "Tyrosine 387 and arginine 404 are critical in the hydrolytic mechanism of Escherichia coli aminopeptidase P."
    
21. A. Bondue, S.-C. Jao, C. Blanpain, M. Parmentier & P. J. LiWang  (2002)  Biochemistry  41(46), 13548-55  "Characterization of the role of the N-loop of MIP-1 beta in CCR5 binding."
    
22. S. Kim, S.-C. Jao, J. S. Laurence & P. J. LiWang  (2001)  Biochemistry  40, 10782-10791  "Structural comparison of monomeric variants of the chemokine MIP-1β having differing ability to bind the receptor CCR5."
    
23. H. Shao, S.-C. Jao, K. Ma & M. G. Zagorski  (1999)  J. Mol. Biol.  285, 755-773  "Solution structures of micelle-bound amyloid β-(1-40) and β-(1-42) peptides of Alzheimer's disease."
    
24. Y. Yang, S.-C. Jao, S. Nanduri, D. W. Starke, J. J. Mieyal & J. Qin  (1998)  Biochemistry  37(49), 17145-56  "Reactivity of the human thioltransferase (glutaredoxin) C7S, C25S, C78S, C82S mutant and NMR solution structure of its glutathionyl mixed disulfide intermediate reflect catalytic specificity."
    
25. S.-C. Jao, K. Ma, J. Talafous, R. Orlando & M. G. Zagorski  (1997)  Amyloid-Journal of Protein Folding Disorders  4, 240-252  "Trifluoroacetic acid pretreatment reproducibly disaggregates the amyloid β-peptide."