中央研究院 生物化學研究所

研究領域與專長

蛋白質核磁共振學 結構生物學 DNA的突變與修復機制

學經歷

• 1993 – 1999   博士, 化學, 紐約州立大學石溪分校
• 1988 – 1992   學士, 化學, 東吳大學

• 2015 – 迄今   研究副技師, 中央研究院 生物化學所
• 2012 – 2015   研究助技師, 中央研究院 生物化學所
• 2005 – 2012   研究助技師, 中央研究院 生物醫學所
• 2003 – 2005   Staff Scientist, 中央研究院 高磁場核磁共振中心
• 1999 – 2003   Research Associate, National Magnetic Resonance Facility at Madison (NMRFAM), Department of Biochemistry, University of Wisconsin at Madison,

主要著作

Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme.  
Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, Wu WJ, Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, Essen LO*, Bessho Y*, Tsai MD* 
Nature chemistry (2022)

Enzymology and Dynamics by Cryogenic Electron Microscopy.  
Tsai MD*, Wu WJ, Ho MC 
Annual review of biophysics (2022)

Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use.  
Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, Wu WJ, Jan JT, Ho MR, Chou CY, Wang AH, Wu CY, Wang JT, Huang KF*, Wu SH* 
Journal of Biomedical Science (2022)

Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures.  
Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*  
Nature Communications (2021)

Human DNA Polymerase μ Can Use a Noncanonical Mechanism for Multiple Mn2+-mediated Functions.  
Chang YK, Huang YP, Liu XX, Ko TP, Bessho Y, Kawano Y, Maestre-Reyna M, Wu WJ*, Tsai MD*  
Journal of the American Chemical Society (2019)

Regulatory cascade involving transcriptional and N-end rule pathways in rice under submergence.  
Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, Wu WJ, Wu JL, Gibbs DJ, Ho MC, Shih MC  
Proceedings of the National Academy of Sciences of the United States of America (2019)

How DNA polymerases catalyze replication and repair with contrasting fidelity.  
Wu WJ, Yang W, Tsai MD  
Nature Reviews Chemistry (2017)

Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ.  
Liu, M.-S., Tsai, H.-Y., Liu, X.-X., Ho, M.-C., Wu, W.-J.*, and Tsai, M.-D.*  
J. Am. Chem. Soc. (2016)

How a low-fidelity DNA polymerase chooses non-watson-crick from watson-crick incorporation.  
Wu WJ, Su MI, Wu JL, Kumar S, Lim LH, Wang CW, Nelissen FH, Chen MC, Doreleijers JF, Wijmenga SS, Tsai MD.  
J. Am. Chem. Soc. (2014)

Stereospecificity of the Reaction Catalyzed by Enoyl-CoA Hydratase.  
Wen-Jin Wu , Yuguo Feng , Xiang He , Hilary A. Hofstein , Daniel P. Raleigh , and Peter J. Tonge  
J. Am. Chem. Soc. (2000)

著作列表

1. Shekhar AC, (Wu WJ), Chen HT*  (2023-05 accepted)  The Journal of biological chemistry  "Mutational and biophysical analyses reveal a TFIIIC binding region in the TFIIF-related Rpc53 subunit of RNA polymerase III."
   
2. Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, (Wu WJ), Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, Essen LO*, Bessho Y*, Tsai MD*  (2022-06)  Nature chemistry  14(6), 677-685  "Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme."
   
3. Tsai MD*, (Wu WJ), Ho MC  (2022-05)  Annual review of biophysics  51, 19-38  "Enzymology and Dynamics by Cryogenic Electron Microscopy."
   
4. Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, (Wu WJ), Jan JT, Ho MR, Chou CY, Wang AH, Wu CY, Wang JT, Huang KF*, Wu SH*  (2022-02)  Journal of Biomedical Science  29(1), 9  "Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use."
   
5. Maestre-Reyna M, Huang WC, (Wu WJ), Singh PK, Hartmann R, Wang PH, Lee CC, Hikima T, Yamamoto M, Bessho Y, Drescher K, Tsai MD, Wang AH*  (2021-02)  IUBMB life  73(2), 418-431  "Vibrio cholerae biofilm scaffolding protein RbmA shows an intrinsic, phosphate-dependent autoproteolysis activity."
   
6. Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, (Wu WJ), Li HW, Ho MC*, Chi P*, Tsai MD*  (2021-01)  Nature Communications  12(1), 115  "Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures."
   
7. Hsieh IS, Gopula B, Chou CC, Wu HY, Chang GD, (Wu WJ), Chang CS, Chu PC, Chen CS  (2019-09)  Journal of medicinal chemistry  62(18), 8497-8510  "Development of Novel Irreversible Pyruvate Kinase M2 Inhibitors."
   
8. LinWu SW, Tu YH, Tsai TY, Maestre-Reyna M, Liu MS, (Wu WJ), Huang JY, Chi HW, Chang WH, Chiou CF, Wang AH, Lee J, Tsai MD  (2019-06)  Communications Biology  2(1), 224  "Thermococcus sp. 9°N DNA polymerase exhibits 3′-esterase activity that can be harnessed for DNA sequencing."
   
9. Chang YK, Huang YP, Liu XX, Ko TP, Bessho Y, Kawano Y, Maestre-Reyna M, (Wu WJ*), Tsai MD  (2019-05)  Journal of the American Chemical Society  141(21), 8489-8502  "Human DNA Polymerase mu Can Use a Noncanonical Mechanism for Multiple Mn(2+)-Mediated Functions."
   
10. Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, (Wu WJ), Wu JL, Gibbs DJ, Ho MC, Shih MC  (2019-02)  Proceedings of the National Academy of Sciences of the United States of America  116(8), 3300-3309  "Regulatory cascade involving transcriptional and N-end rule pathways in rice under submergence."
    
11. Chen ES, Weng JH, Chen YH, Wang SC, Liu XX, Huang WC, Matsui T, Kawano Y, Liao JH, Lim LH, Bessho Y, Huang KF, (Wu WJ), Tsai MD  (2017-09)  Biochemistry  56(38), 5112-5124  "Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation"
    
12. (Wu Wen-Jin), Yang Wei, Tsai Ming-Daw  (2017)  Nature Reviews Chemistry  1(9)  "How DNA polymerases catalyse replication and repair with contrasting fidelity"
    
13. Liu MS, Tsai HY, Liu XX, Ho MC, (Wu WJ*), Tsai MD*  (2016-02)  Journal of the American Chemical Society  138(7), 2389-2398  "Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ"
    
14. (Wu WJ), Su MI, Wu JL, Kumar S, Lim LH, Wang CW, Nelissen FH, Chen MC, Doreleijers JH, Wijmenga SS, Tsai MD  (2014-04)  Journal of the American Chemical Society  136(13), 4927-4937  "How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation."
    
15. Maestre-Reyna M, (Wu WJ), Wang AH  (2013-12)  PLoS One  8(12), e82458  "Structural Insights into RbmA, a Biofilm Scaffolding Protein of V. Cholerae"
    
16. Wang CC, Huang HB, Tsay HJ, Shiao MS, (Wu WJ), Cheng YC, Lin TH  (2012-06)  Journal of biomolecular structure & dynamics  30(2), 160-169  "Characterization of Abeta aggregation mechanism probed by congo red."
    
17. Jiang I, Tsai CK, Chen SC, Wang SH, Amiraslanov I, Chang CF, (Wu WJ), Tai JH, Liaw YC, Huang TH  (2011)  Nucleic acids research  39(20), 8992-9008  "Molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a Myb protein from the protozoan parasite Trichomonas vaginalis."
    
18. Sue SC, Lee WT, Tien SC, Lee SC, Yu JG, (Wu WJ), Wu WG, Huang TH  (2007)  Journal of molecular biology  367(2), 456-472  "PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin."
    
19. (Wu WJ), Tyukhtenko SI, Huang TH  (2006)  Magnetic resonance in chemistry : MRC  44(11), 1037-1040  "Direct NMR resonance assignments of the active site histidine residue in Escherichia coli thioesterase I/protease I/lysophospholipase L1."
    
20. Chang CK, Sue SC, Yu TH, Hsieh CM, Tsai CK, Chiang YC, Lee SJ, Hsiao HH, (Wu WJ), Chang WL, Lin CH, Huang TH  (2006)  Journal of biomedical science  13(1), 59-72  "Modular organization of SARS coronavirus nucleocapsid protein."
    
21. Wu KP, Chang JM, Chen JB, Chang CF, (Wu WJ), Huang TH, Sung TY, Hsu WL  (2006)  Journal of computational biology : a journal of computational molecular cell biology  13(2), 229-244  "RIBRA--an error-tolerant algorithm for the NMR backbone assignment problem."
    
22. Chang CK, Sue SC, Yu TH, Hsieh CM, Tsai CK, Chiang YC, Lee SJ, Hsiao HH, (Wu WJ), Chang CF, Huang TH  (2005)  FEBS letters  579(25), 5663-8  "The dimer interface of the SARS coronavirus nucleocapsid protein adapts a porcine respiratory and reproductive syndrome virus-like structure."
    
23. (Wu WJ), Vidugiris G, Mooberry ES, Westler WM, Markley JL  (2003)  Journal of magnetic resonance  164(1), 84-91  "Mixing apparatus for preparing NMR samples under pressure."
    
24. (Wen-Jin Wu) , Yuguo Feng , Xiang He , Hilary A. Hofstein , Daniel P. Raleigh , and Peter J. Tonge   (2000-04)  J. Am. Chem. Soc.  122(17), 3987-3994  "Stereospecificity of the Reaction Catalyzed by Enoyl-CoA Hydratase"
    
25. Luisi DL, (Wu WJ), Raleigh DP  (1999)  Journal of molecular biology  287(2), 395-407  "Conformational analysis of a set of peptides corresponding to the entire primary sequence of the N-terminal domain of the ribosomal protein L9: evidence for stable native-like secondary structure in the unfolded state."
    
26. Sato S, Kuhlman B, (Wu WJ), Raleigh DP  (1999)  BIOCHEMISTRY  38(17), 5643-50  "Folding of the multidomain ribosomal protein L9: the two domains fold independently with remarkably different rates."
    
27. (W.-J. Wu) and D.P. Raleigh  (1998-02)  JOURNAL OF ORGANIC CHEMISTRY  63(19), 6689-6698  "Conformational Heterogeneity about Pipecolic Acid Peptide Bonds: Conformational, Thermodynamic and Kinetic Aspects"
    
28. (W.-J. Wu), P.J. Tonge and D.P. Raleigh  (1998-02)  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY  120(39), 9988-9994  "Stereospecific 1H and 13C NMR Assignments of Crotonyl- Coenzyme A and Hexadienoyl-Coenzyme A: Conformational Analysis and Comparison with Protein:CoA Complexes"
    
29. (Wu WJ), Raleigh DP  (1998)  Biopolymers  45(5), 381-94  "Local control of peptide conformation: stabilization of cis proline peptide bonds by aromatic proline interactions."
    
30. Kuhlman B, Boice JA, (Wu WJ), Fairman R, Raleigh DP  (1997)  BIOCHEMISTRY  36(15), 4607-4615  "Calcium binding peptides from alpha-lactalbumin: implications for protein folding and stability."
    
31. (Wu WJ), Anderson VE, Raleigh DP, Tonge PJ  (1997)  Biochemistry  36(8), 2211-20  "Structure of hexadienoyl-CoA bound to enoyl-CoA hydratase determined by transferred nuclear Overhauser effect measurements: mechanistic predictions based on the X-ray structure of 4-(chlorobenzoyl)-CoA dehalogenase."
    
32. (Wu WJ), Hsu JY, Wen YS and Shyu SG  (1995-06)  JOURNAL OF THE CHINESE CHEMICAL SOCIETY  42(3), 533-535  "Synthesis of Heterobimetallic Phosphido-Bridged W-Mo Complexes with Chelating dppe and dppm Ligands"
    
33. S.-G. Shyu, (W.-J, Wu), Y.-S. Wen, S.-M. Peng and G.H. Lee  (1995-02)  JOURNAL OF ORGANOMETALLIC CHEMISTRY  489(1-2), 113-122  "Synthesis, Structure and Substitution Reactions of the Binuclear Phosphido-Bridged Complex Cp(CO)2W(m-PPh2)W(CO)5"
    
34. S.-G. Shyu, J.-Y. Hsu, P.-J., Lin, (W.-J. Wu), S.-M. Peng, G.-H. Lee and Y.-S. Wen  (1994-02)  ORGANOMETALLICS  13(5), 1699-1710  "Synthesis, Structure, Fluxional Behavior, and Addition Reaction of the Metal-Metal- Bonded Heterobimetallic Phosphido-Bridged Complex CpW(CO)2(m-PPh2)Mo(CO)5"