中央研究院 生物化學研究所

研究領域與專長

蛋白質核磁共振學 結構生物學 DNA的突變與修復機制

學經歷

• 1993 – 1999   博士, 化學, 紐約州立大學石溪分校
• 1988 – 1992   學士, 化學, 東吳大學

• 2015 – 迄今   研究副技師, 中央研究院 生物化學所
• 2012 – 2015   研究助技師, 中央研究院 生物化學所
• 2005 – 2012   研究助技師, 中央研究院 生物醫學所
• 2003 – 2005   Staff Scientist, 中央研究院 高磁場核磁共振中心
• 1999 – 2003   Research Associate, National Magnetic Resonance Facility at Madison (NMRFAM), Department of Biochemistry, University of Wisconsin at Madison,

主要著作

Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme.  
Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, Wu WJ, Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, Essen LO*, Bessho Y*, Tsai MD* 
Nature chemistry (2022)

Enzymology and Dynamics by Cryogenic Electron Microscopy.  
Tsai MD*, Wu WJ, Ho MC 
Annual review of biophysics (2022)

Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use.  
Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, Wu WJ, Jan JT, Ho MR, Chou CY, Wang AH, Wu CY, Wang JT, Huang KF*, Wu SH* 
Journal of Biomedical Science (2022)

Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures.  
Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*  
Nature Communications (2021)

Human DNA Polymerase μ Can Use a Noncanonical Mechanism for Multiple Mn2+-mediated Functions.  
Chang YK, Huang YP, Liu XX, Ko TP, Bessho Y, Kawano Y, Maestre-Reyna M, Wu WJ*, Tsai MD*  
Journal of the American Chemical Society (2019)

Regulatory cascade involving transcriptional and N-end rule pathways in rice under submergence.  
Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, Wu WJ, Wu JL, Gibbs DJ, Ho MC, Shih MC  
Proceedings of the National Academy of Sciences of the United States of America (2019)

How DNA polymerases catalyze replication and repair with contrasting fidelity.  
Wu WJ, Yang W, Tsai MD  
Nature Reviews Chemistry (2017)

Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ.  
Liu, M.-S., Tsai, H.-Y., Liu, X.-X., Ho, M.-C., Wu, W.-J.*, and Tsai, M.-D.*  
J. Am. Chem. Soc. (2016)

How a low-fidelity DNA polymerase chooses non-watson-crick from watson-crick incorporation.  
Wu WJ, Su MI, Wu JL, Kumar S, Lim LH, Wang CW, Nelissen FH, Chen MC, Doreleijers JF, Wijmenga SS, Tsai MD.  
J. Am. Chem. Soc. (2014)

Stereospecificity of the Reaction Catalyzed by Enoyl-CoA Hydratase.  
Wen-Jin Wu , Yuguo Feng , Xiang He , Hilary A. Hofstein , Daniel P. Raleigh , and Peter J. Tonge  
J. Am. Chem. Soc. (2000)

著作列表