專任研究人員
ylshih10@gate.sinica.edu.tw +886-2-3366-4071,2366-5599 ext 5543
史有伶 副研究員
臺大生化所 405室
研究領域與專長
學經歷
主要著作
著作列表

研究領域與專長

我們從事細菌生理學研究，課題包括細菌生長分裂與形態生成， 相關過程在生命週期中極為關鍵但脆弱，因此研究成果可能揭示防治細菌感染的潛在標的與策略的學理基礎。另一課題為探討影響宿主-病原菌交互作用中的細菌表面分子。簡介如下：

(1)代謝作用對細菌生長分裂的調節     
      細菌的生長和分裂狀態決定形態的生成，與代謝作用之間的協同可能是細胞生理上的固有特性，用以提高對生存環境的適應性。因此，我們研究代謝酵素與細胞生長和分裂之間在功能上的偶合現象。

(2)細菌細胞壁生合成與重塑     
      參與肽聚醣生合成的蛋白質複合體存在細胞膜環境中，其研究具有複雜度與挑戰性。我們運用細菌基因工程、定量蛋白體分析、和蛋白質生化等方法，來檢視複合體中的蛋白質組成與交互作用，找出具顯著性的新穎生理功能和交互作用蛋白。

(3)病原細菌進行胞內感染時的表面醣修飾     
      在這個研究課題中，我們研究李斯特菌進行胞內感染時表面的醣基化，如何與宿主細胞質中的半乳糖結合凝集素產生交互作用，影響宿主的防禦機制。

學經歷

  • 1996 – 1999   博士, 生物化學系, 英國劍橋大學
  • 1992 – 1994   碩士, 植物病蟲害學系, 國立臺灣大學
  • 1988 – 1992   學士, 植物病蟲害學系, 國立臺灣大學
  • 2014 – 迄今   副研究員, 中研院生化所
  • 2006 – 2014   助研究員, 中研院生化所
  • 2004 – 2006   講師, 美國康乃迪克大學健康中心分子生物微生物及結構生物學系
  • 1999 – 2003   博士後研究, 美國康乃迪克大學健康中心分子生物微生物及結構生物學系

主要著作

Active transport of membrane components by self-organization of the Min proteins. 
Shih YL*, Huang LT, Tu YM, Lee BF, Bau YC, Hong CY, Lee HL, Shih YP, Hsu MF, Lu ZX, Chen JS, Chao L* 
Biophysical Journal (2019)

Quantitative proteomics analysis reveals the Min system of Escherichia coli modulates reversible protein association with the inner membrane. 
Lee HL, Chiang IC, Liang SY, Lee DY, Chang GD, KY Wang, Lin SY, Shih YL 
MOLECULAR & CELLULAR PROTEOMICS (2016)

Self-Assembly of MinE on the Membrane Underlies Formation of the MinE-Ring to Sustain Function of the E. coli Min System. 
Zheng M, Chiang YL, Lee HL, Kong LR, Hsu ST, Hwang IS, Rothfield LI and Shih YL 
J Biol Chem (2014)

The N-terminal amphipathic helix of the topological specificity factor MinE is associated with shaping membrane curvature. 
Shih YL, Huang KF, Lai HM, Liao JH, Lee CS, Chang CM, Mak HM, Hsieh CW & Lin CC 
PLoS One (2011)

Direct MinE-membrane interaction contributes to the proper localization of MinDE in E. coli. 
Hsieh CW, Lai HM, Lin CC, Lin TY, Hsieh TS, Shih YL 
Molecular Microbiology (2010)

The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation. 
Shih YL*, Kawagishi I and Rothfield LI* 
Mol Microbiol (2005)

Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles. 
Shih YL, Le T, Rothfield LI* 
Proc Natl Acad Sci USA (2003)

Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains. 
Shih YL, Fu X, King GF, Le T and Rothfield LI* 
EMBO J (2002)

著作列表

  1. Nguyen HTV, Chen X, Parada C, Luo AC, Shih O, Jeng US, Huang CY, (Shih YL)*, Ma C*.  (2023-04)  NATURE COMMUNICATIONS  14, 1903  "Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome"
  2. Hsieh PY, Meng FC, Guo CW, Hu KH, (Shih YL)*, Cheng WC*  (2021-12)  CHEMBIOCHEM  22(24), 3462-3468  "Harnessing fluorescent moenomycin A antibiotics for bacterial cell wall imaging studies."
  3. Sun YJ, Bai F, Luo AC, Zhuang XY, Lin TS, (Shih YL), Lo CJ*  (2021-04)  NATURE COMMUNICATIONS  12(1), 2160  "Probing bacterial cell wall growth by tracing wall-anchored protein complexes"
  4. Huang WC, Hashimoto M, (Shih YL), Wu CC, Lee MF, Chen YL, Wu JJ, Wang MC, Lin WH, Hong MY, Teng CH*  (2020-12)  FRONTIERS IN MICROBIOLOGY  11, 3234  "Peptidoglycan endopeptidase Spr of uropathogenic E. coli contributes to kidney infections and competitive fitness during bladder colonization"
  5. Wu CF, Lien YW, Bondage D, Lin JS, Pilhofer M, (Shih YL), Chang JH, Lai EM*  (2020-01)  EMBO Reports  21(1), e47961  "Effector loading onto the VgrG carrier activates type VI secretion system assembly."
  6. (Shih YL)*, Huang LT, Tu YM, Lee BF, Bau YC, Hong CY, Lee HL, Shih YP, Hsu MF, Lu ZX, Chen JS, Chao L*  (2019-04)  BIOPHYSICAL JOURNAL  116(8), 1469-1482  "Active Transport of Membrane Components by Self-Organization of the Min Proteins."
  7. Chen WJ, Kuo TY, Hsieh FC, Chen PY, Wang CS, (Shih YL), Lai YM, Liu JR, Yang YL, and Shih MC*  (2016-09)  Scientific Reports  6, 32950  "Involvement of type VI secretion system in secretion of iron chelator pyoverdine in Pseudomonas taiwanensis"
  8. Liang SY, Lin SY, Chiang IC, (Shih YL*)  (2016-05)  Data in Brief  8, 304-307  "Quantitative inner membrane proteome datasets of the wild-type and the Δmin mutant of Escherichia coli"
  9. Liao JH, Chien CT, Wu, HY, Huang KF, Wang I, Ho MR, Tu IF, Lee IM, Li W, (Shih YL), Wu CY, Lukyanov P, Hsu STD, Wu SH*  (2016-04)  J Am Chem Soc  138(14), 4787-4795  "A multivalent marine lectin from Crenomytilus grayanus possesses anti-cancer activity through recognizing globotriose Gb3"
  10. Lee HL, Chiang IC, Liang SY, Lee DY, Chang GD, KY Wang, Lin SY, (Shih YL*)  (2016-02)  MOLECULAR & CELLULAR PROTEOMICS  15, 1572-1583  "Quantitative proteomics analysis reveals the Min system of Escherichia coli modulates reversible protein association with the inner membrane"
  11. Chiang YL, Chang YC, Chiang IC, Mak HM, Hwang IS*, (Shih YL)*  (2015-11)  PloS one  10(11), e0142506  "Atomic Force Microscopy Characterization of Protein Fibrils Formed by the Amyloidogenic Region of the Bacterial Protein MinE on Mica and a Supported Lipid Bilayer."
  12. Rujiviphat J, Wong MK, Won A, (Shih YL), Yip CM and McQuibban AG*  (2015-03)  J Mol Biol  427(16), 2595-2598  "Mitochondrial Genome Maintenance 1 (Mgm1) Protein alters membrane topology and promotes local membrane bending"
  13. Zheng M, Chiang YL, Lee HL, Kong LR, Hsu ST, Hwang IS, Rothfield LI, (Shih YL)*   (2014-08)  J Biol Chem  289(31), 21252-21266  "Self-Assembly of MinE on the Membrane Underlies Formation of the MinE-Ring to Sustain Function of the E. coli Min System"
  14. (Shih YL)*, Zheng M  (2013-12)  Environmental Microbiology  15(12), 3229-3239  "Spatial control of the cell division site by the Min system in E. coli"
  15. (Shih YL)＊, Huang KF, Lai HM, Liao JH, Lee CS, Chang CM, Mak HM, Hsieh CW & Lin CC  (2011)  PLoS One  6(6), e21425  "The N-terminal amphipathic helix of the topological specificity factor MinE is associated with shaping membrane curvature"
  16. Hsieh CW, Lai HM, Lin CC, Lin TY, Hsieh TS, (Shih YL)＊  (2010)  MOLECULAR MICROBIOLOGY  75(2), 499-512  "Direct MinE-membrane interaction contributes to the proper localization of MinDE in E. coli"
  17. Vats P, Shih YL, Rothfield L  (2009)  Molecular microbiology  72(1), 170-182  "Assembly of the MreB-associated cytoskeletal ring of Escherichia coli."
  18. Pradel N, Santini CL, Bernadac A, Shih YL, Goldberg MB, Wu LF  (2007)  Biochemical and biophysical research communications  353(2), 493-500  "Polar positional information in Escherichia coli spherical cells."
  19. Y-L Shih, LI Rothfield  (2006)  Microbiology and Molecular Biology Reviews  70, 729-754  "The bacterial cytoskeleton"
  20. LI Rothfield, A Taghbalout and Y-L Shih  (2005)  Nat Rev Microbiol  3, 959-968  "Spatial control of bacterial division-site placement"
  21. Y-L Shih, I Kawagishi and LI Rothfield  (2005)  Mol Microbiol  58, 917-928  "The MreB and Min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation"
  22. Shih YL, Le T, Rothfield L  (2003)  Proceedings of the National Academy of Sciences of the United States of America  100(13), 7865-7870  "Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles."
  23. Y-L Shih, X Fu, GF King , T Le and LI Rothfield  (2002)  EMBO J  21, 3347-3357  "Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains"
  24. Rothfield LI, Shih YL, King G  (2001)  Cell  106(1), 13-16  "Polar explorers: membrane proteins that determine division site placement."
  25. Fu X, Shih YL, Zhang Y, Rothfield LI  (2001)  Proceedings of the National Academy of Sciences of the United States of America  98(3), 980-985  "The MinE ring required for proper placement of the division site is a mobile structure that changes its cellular location during the Escherichia coli division cycle."
  26. King GF, Shih YL, Maciejewski MW, Bains NP, Pan B, Rowland SL, Mullen GP, Rothfield LI  (2000)  Nature Structural Biology  7(11), 1013-1017  "Structural basis for the topological specificity function of MinE."
  27. Shih YL, Harris SJ, Borner G, Rivet MM, Salmond GP  (1999)  Environmental microbiology  1(6), 535-547  "The hexY genes of Erwinia carotovora ssp. carotovora and ssp. atroseptica encode novel proteins that regulate virulence and motility co-ordinately."
  28. Harris SJ, Shih YL, Bentley SD, Salmond GP  (1998)  Molecular microbiology  28(4), 705-717  "The hexA gene of Erwinia carotovora encodes a LysR homologue and regulates motility and the expression of multiple virulence determinants."
  29. Toth I, Mulholland V, Copper V, Bentley S, Shih Y-L, Perombelon M, and Salmond GPC  (1997)  Microbiology  143, 2433-2438  "Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage φM1"