中央研究院 生物化學研究所

研究領域與專長

神經元和神經膠質細胞是構成人類大腦的主要細胞類型。不同神經元之間以及神經元和神經膠質之間的緊密交流對於控制人類大腦的發育、維持和活動至關重要。這些溝通的失能往往會導致各式各樣嚴重的神經發育性、精神性和神經退化性疾​​病，其中大多數疾病仍在日漸積累當中，但卻無法治愈。因此，本實驗室旨在了解大腦如何正常運作以及神經系統疾病是如何被發展出來的，從而期許能發展出可行性高的治療對策。

實驗室目前研究方向如下: 
1)快速神經傳遞中突觸小泡再循環的分子機制。 
2)神經元與膠質細胞緊密交流在人類健康和疾病中的重要性。 
3)細胞內聚醣和聚醣結合蛋白在神經退化性疾​​病中的作用。

為此，本研究團隊主要使用果蠅作為強力的遺傳模式生物系統，也結合大鼠培養神經元、人類神經元細胞株、電生理學、CRISPR基因編輯、RNAi技術、生物化學、共聚焦顯微鏡、超高解析度顯微鏡、透射式電子顯微鏡、體外重組系統和冷凍電顯。

學經歷

• 2001 – 2005   博士, 遺傳所, 國立陽明大學
• 1999 – 2001   碩士, 遺傳所, 國立陽明大學
• 1995 – 1999   學士, 生物系, 國立成功大學

• 2020 – 迄今   副研究員, 生物化學所, 中央研究院
• 2011 – 2020   助研究員, 生物化學所, 中央研究院
• 2006 – 2011   博士後, 分子及人類遺傳所, 美國貝勒醫學院
• 2005 – 2006   博士後, 分子生物所, 中央研究院

主要著作

ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival. 
Kim J, Kim S, Nahm M, Li TN, Lin HC, Kim YD, Lee J, Yao CK*, Lee S* 
Journal of Cell Biology (2021)

A positive feedback loop between Flower and PI(4,5)P2 at periactive zones controls bulk endocytosis in Drosophila. 
Li TN, Chen YJ, Wang YT, Lin HC, Lu TY, Yao CK* 
Elife (2020)

Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development. 
Lin SS, Hsieh TL, Liou GG, Li TN, Lin HC, Chang CW, Wu HY, Yao CK, Liu YW 
Cell Reports (2020)

A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the Drosophila motor system. 
Peng JJ, Lin SH, Liu YT, Lin HC, Li TN, Yao CK 
eLife (2019)

A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis. 
Yao CK, Liu YT, Lee IC, Wang YT, Wu PY 
PLoS biology (2017)

Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production. 
Sandoval H, Yao CK, Chen K, Jaiswal M, Donti T, Lin YQ, Bayat V, Xiong B, Zhang K, David G, Charng WL, Yamamoto S, Duraine L, Graham BH, Bellen HJ 
eLife (2014)

A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis. 
Yao CK, Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG, Bellen HJ 
Cell (2009)

Rab3 GTPase lands Bruchpilot. 
Giagtzoglou N, Mahoney T, Yao CK, Bellen HJ 
Neuron (2009)

straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit. 
Ly CV, Yao CK, Verstreken P, Ohyama T, Bellen HJ 
The Journal of cell biology (2008)

Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development. 
Yao JG, Sun YH 
The EMBO journal (2005)

著作列表

1. Park HG, Kim YD, Cho E, Lu TY, (Yao CK), Lee J, Lee S  (2022-10)  The Journal of cell biology  221(10), e202203048  "Vav independently regulates synaptic growth and plasticity through distinct actin-based processes."
   
2. Kim J, Kim S, Nahm M, Li TN, Lin HC, Kim YD, Lee J, (Yao CK)*, Lee S*  (2021-03)  J Cell Biol.  220(5), e202007112  "ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival."
   
3. Li TN, Chen YJ, Wang YT, Lin HC, Lu TY, (Yao CK)*  (2020-12)  Elife  9, e60125  "A positive feedback loop between Flower and PI(4,5)P2 at periactive zones controls bulk endocytosis in Drosophila."
   
4. Lin SS, Hsieh TL, Liou GG, Li TN, Lin HC, Chang CW, Wu HY, (Yao CK), Liu YW  (2020-10)  Cell Reports  33(4), 108310  "Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development."
   
5. Amartuvshin O, Lin CH, Hsu SC, Kao SH, Chen A, Tang WC, Chou HL, Chang DL, Hsu YY, Hsiao BS, Rastegari E, Lin KY, Wang YT, (Yao CK), Chen GC, Chen BC, Hsu HJ  (2020-08)  Aging cell  19(8), e13191  "Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss."
   
6. Peng JJ, Lin SH, Liu YT, Lin HC, Li TN, (Yao CK)  (2019-07)  eLife  8, e47372  "A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the Drosophila motor system."
   
7. (Yao CK), Liu YT, Lee IC, Wang YT, Wu PY  (2017-04)  PLoS biology  15(4), e2000931  "A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis."
   
8. Tsai YC, Grimm S, Chao JL, Wang SC, Hofmeyer K, Shen J, Eichinger F, Michalopoulou T, (Yao CK), Chang CH, Lin SH, Sun YH, Pflugfelder GO  (2015-03)  PloS one  10(3), e0120236  "Optomotor-blind negatively regulates Drosophila eye development by blocking Jak/STAT signaling."
   
9. Sandoval H, (Yao CK), Chen K, Jaiswal M, Donti T, Lin YQ, Bayat V, Xiong B, Zhang K, David G, Charng WL, Yamamoto S, Duraine L, Graham BH, Bellen HJ  (2014-10)  eLife  3, e03558  "Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production."
   
10. (Yao CK), Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG, Bellen HJ  (2009)  Cell  138(5), 947-960  "A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis."
    
11. Giagtzoglou N, Mahoney T, (Yao CK), Bellen HJ  (2009)  Neuron  64(5), 595-597  "Rab3 GTPase lands Bruchpilot."
    
12. (Yao JG), Weasner BM, Wang LH, Jang CC, Weasner B, Tang CY, Salzer CL, Chen CH, Hay B, Sun YH, Kumar JP  (2008)  Developmental biology  315(2), 535-551  "Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila."
    
13. Ly CV, (Yao CK), Verstreken P, Ohyama T, Bellen HJ  (2008)  The Journal of cell biology  181(1), 157-170  "straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit."
    
14. Tsai YC, (Yao JG), Chen PH, Posakony JW, Barolo S, Kim J, Sun YH  (2007)  Developmental biology  306(2), 760-771  "Upd/Jak/STAT signaling represses wg transcription to allow initiation of morphogenetic furrow in Drosophila eye development."
    
15. (Yao JG), Sun YH  (2005)  The EMBO journal  24(14), 2602-2612  "Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development."