IBC - 姚季光

研究領域與專長

大腦控管著人體重要的行為，例如:學習、記憶、情緒、感覺、運動等。大腦主要是由具有龐大數量以及複雜種類的神經元細胞與神經膠質細胞組成，它們透過緊密細胞間相互溝通不僅操控著上述行為，也支配著大腦早期發育以及晚期功能維持。因此，這些溝通的失能往往能發展出各式各樣嚴重的神經發育性、精神性和退化性疾病，其中大多數病人仍在日漸積累當中，但不幸的是，目前缺乏治愈方法。基於此，姚季光老師研究團隊致力於闡釋大腦正常運作之謎，破解造成神經元發育與退化疾病之致病機轉，從而期許能開發出可行性高的治療方法。

研究方向
1) 探索嶄新的神經元細胞 (Neurons)與神經膠質細胞(Glial cells)間溝通途徑，以及它在神經元退化疾病中的角色。
2) 說明內溶酶體系統(Endolysosomal system)失調在神經元發育疾病中的致病原因。
3) 建立早期診斷基因突變與罕見神經疾病發生關聯性之平台，以及基因突變致病機轉之研究。
4) 探索快速神經傳遞中突觸小泡回收 (Synaptic vesicle recycle)機制。

研究技術
本團隊研究主要藉由果蠅模式生物強力的遺傳學系統，也結合初代培養大鼠神經元細胞、人類神經元細胞株、電生理學、CRISPR基因編輯、RNAi技術、生物化學、共聚焦顯微鏡、超高解析度顯微鏡、透射式電子顯微鏡、體外蛋白質與膜質重組系統和冷凍電子顯微鏡。

學經歷

2001 – 2005 博士, 遺傳所, 國立陽明大學
1999 – 2001 碩士, 遺傳所, 國立陽明大學
1995 – 1999 學士, 生物系, 國立成功大學

2020 – 迄今副研究員, 生物化學所, 中央研究院
2011 – 2020 助研究員, 生物化學所, 中央研究院
2006 – 2011 博士後, 分子及人類遺傳所, 美國貝勒醫學院
2005 – 2006 博士後, 分子生物所, 中央研究院

主要著作

ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival.
Kim J, Kim S, Nahm M, Li TN, Lin HC, Kim YD, Lee J, Yao CK*, Lee S*
Journal of Cell Biology (2021)

A positive feedback loop between Flower and PI(4,5)P2 at periactive zones controls bulk endocytosis in Drosophila.
Li TN, Chen YJ, Wang YT, Lin HC, Lu TY, Yao CK*
Elife (2020)

Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development.
Lin SS, Hsieh TL, Liou GG, Li TN, Lin HC, Chang CW, Wu HY, Yao CK, Liu YW
Cell Reports (2020)

A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the Drosophila motor system.
Peng JJ, Lin SH, Liu YT, Lin HC, Li TN, Yao CK
eLife (2019)

A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis.
Yao CK, Liu YT, Lee IC, Wang YT, Wu PY
PLoS biology (2017)

Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production.
Sandoval H, Yao CK, Chen K, Jaiswal M, Donti T, Lin YQ, Bayat V, Xiong B, Zhang K, David G, Charng WL, Yamamoto S, Duraine L, Graham BH, Bellen HJ
eLife (2014)

A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis.
Yao CK, Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG, Bellen HJ
Cell (2009)

Rab3 GTPase lands Bruchpilot.
Giagtzoglou N, Mahoney T, Yao CK, Bellen HJ
Neuron (2009)

straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit.
Ly CV, Yao CK, Verstreken P, Ohyama T, Bellen HJ
The Journal of cell biology (2008)

Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development.
Yao JG, Sun YH
The EMBO journal (2005)

著作列表

Park HG, Kim YD, Cho E, Lu TY, (Yao CK), Lee J, Lee S (2022-10) The Journal of cell biology 221(10), e202203048 "Vav independently regulates synaptic growth and plasticity through distinct actin-based processes."
Kim J, Kim S, Nahm M, Li TN, Lin HC, Kim YD, Lee J, (Yao CK)*, Lee S* (2021-03) J Cell Biol. 220(5), e202007112 "ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival."
Li TN, Chen YJ, Wang YT, Lin HC, Lu TY, (Yao CK)* (2020-12) Elife 9, e60125 "A positive feedback loop between Flower and PI(4,5)P2 at periactive zones controls bulk endocytosis in Drosophila."
Lin SS, Hsieh TL, Liou GG, Li TN, Lin HC, Chang CW, Wu HY, (Yao CK), Liu YW (2020-10) Cell Reports 33(4), 108310 "Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development."
Amartuvshin O, Lin CH, Hsu SC, Kao SH, Chen A, Tang WC, Chou HL, Chang DL, Hsu YY, Hsiao BS, Rastegari E, Lin KY, Wang YT, (Yao CK), Chen GC, Chen BC, Hsu HJ (2020-08) Aging cell 19(8), e13191 "Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss."
Peng JJ, Lin SH, Liu YT, Lin HC, Li TN, (Yao CK) (2019-07) eLife 8, e47372 "A circuit-dependent ROS feedback loop mediates glutamate excitotoxicity to sculpt the Drosophila motor system."
(Yao CK), Liu YT, Lee IC, Wang YT, Wu PY (2017-04) PLoS biology 15(4), e2000931 "A Ca2+ channel differentially regulates Clathrin-mediated and activity-dependent bulk endocytosis."
Tsai YC, Grimm S, Chao JL, Wang SC, Hofmeyer K, Shen J, Eichinger F, Michalopoulou T, (Yao CK), Chang CH, Lin SH, Sun YH, Pflugfelder GO (2015-03) PloS one 10(3), e0120236 "Optomotor-blind negatively regulates Drosophila eye development by blocking Jak/STAT signaling."
Sandoval H, (Yao CK), Chen K, Jaiswal M, Donti T, Lin YQ, Bayat V, Xiong B, Zhang K, David G, Charng WL, Yamamoto S, Duraine L, Graham BH, Bellen HJ (2014-10) eLife 3, e03558 "Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production."
(Yao CK), Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG, Bellen HJ (2009) Cell 138(5), 947-960 "A synaptic vesicle-associated Ca2+ channel promotes endocytosis and couples exocytosis to endocytosis."
Giagtzoglou N, Mahoney T, (Yao CK), Bellen HJ (2009) Neuron 64(5), 595-597 "Rab3 GTPase lands Bruchpilot."
(Yao JG), Weasner BM, Wang LH, Jang CC, Weasner B, Tang CY, Salzer CL, Chen CH, Hay B, Sun YH, Kumar JP (2008) Developmental biology 315(2), 535-551 "Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila."
Ly CV, (Yao CK), Verstreken P, Ohyama T, Bellen HJ (2008) The Journal of cell biology 181(1), 157-170 "straightjacket is required for the synaptic stabilization of cacophony, a voltage-gated calcium channel alpha1 subunit."
Tsai YC, (Yao JG), Chen PH, Posakony JW, Barolo S, Kim J, Sun YH (2007) Developmental biology 306(2), 760-771 "Upd/Jak/STAT signaling represses wg transcription to allow initiation of morphogenetic furrow in Drosophila eye development."
(Yao JG), Sun YH (2005) The EMBO journal 24(14), 2602-2612 "Eyg and Ey Pax proteins act by distinct transcriptional mechanisms in Drosophila development."