演講研討會公告
Engineering protein-based biosensors for fluorescence imaging of cell signalling and metabolism
2019-07-17 11:00 ~ 2019-07-17 12:00
地點: 生化所209室
主講人: Dr. Robert E. Campbell
主講人背景: 日本東京大學化學研究所教授
主講人網站: https://www.chem.s.u-tokyo.ac.jp/campbell/
演講主持人: 呂桐睿特聘研究員
演講摘要:
The Campbell research group is focused on the use of protein engineering for the development of fluorescent proteins and fluorescent protein-based indicators for live cell imaging. Protein engineering using a combination of rational protein design and directed protein evolution has proven to be an effective and versatile approach for generating new genetically encoded fluorescent probes. Indeed, by exploiting iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and low throughput testing of promising variants in mammalian cells, the Campbell group has developed a growing selection of fluorescent protein-based tools with improved brightness, photostability, and biosensing or photoconversion properties. In this seminar I will present some of our most recent efforts to engineer an improved generation of indicators. Specifically, I will provide an update on the expanding palette of calcium ion reporters our lab has been developing, and describe how we are using similar engineering efforts to make reporters for neurotransmitters, ions, and key metabolites.-Dr. Robert E. Campbell
洽詢人員: 劉小姐
洽詢電話: 02-27855696#2061
洽詢信箱: liukchun@gate.sinica.edu.tw