2019-07-17 11:00 ~ 2019-07-17 12:00
Venue: IBC R209
Speaker: Dr. Robert E. Campbell
Current Position: Professor, Department of Chemistry, School of Science, The University of Tokyo, Japan
Host: Dr. Todd L. Lowary
Abstract:
The Campbell research group is focused on the use of protein engineering for the development of fluorescent proteins and fluorescent protein-based indicators for live cell imaging. Protein engineering using a combination of rational protein design and directed protein evolution has proven to be an effective and versatile approach for generating new genetically encoded fluorescent probes. Indeed, by exploiting iterative cycles of high-throughput fluorescence image-based screening of bacterial colonies, and low throughput testing of promising variants in mammalian cells, the Campbell group has developed a growing selection of fluorescent protein-based tools with improved brightness, photostability, and biosensing or photoconversion properties. In this seminar I will present some of our most recent efforts to engineer an improved generation of indicators. Specifically, I will provide an update on the expanding palette of calcium ion reporters our lab has been developing, and describe how we are using similar engineering efforts to make reporters for neurotransmitters, ions, and key metabolites.-Dr. Robert E. Campbell
Contact person: Ms. Nancy Liu
Tel: 02-27855696#2061
Email: liukchun@gate.sinica.edu.tw
Institute of Biological Chemistry, Academia Sinica
