演講研討會公告
Context-specific functions of CDK8
2019-12-13 11:00 ~ 2019-12-13 12:00
地點: 生化所209室
主講人: Dr. Jun-yuan Ji
主講人背景: 美國德克薩斯州A&M大學分子與細胞醫學副教授
主講人網站: https://medicine.tamhsc.edu/faculty/ji.html
演講主持人: 陳光超副研究員
演講摘要:
Using the fruit fly Drosophila melanogaster and cultured mammalian cells as the experimental systems,we are interested in understanding the context-specific roles of the CDK8 module, a highly conserved module of the transcription cofactor Mediator complex, which bridges DNA-bound transcription factors and RNA polymerase II (Pol II) in eukaryotes. The four subunits of the CDK8 module - CDK8, Cyclin C, MED12, and MED13 - are either mutated or amplified in cardiovascular diseases and a number of human cancers. Elucidating the function and regulation of the CDK8 module in different biological contexts is essential to understanding the pathological consequences of its misregulation and to the design of clinical strategies to target the kinase activities of CDK8 in treating these diseases. We have identified two new downstream targets of CDK8, SREBP (sterol regulatory element-binding protein) and EcR (ecdysone receptor), as well as one upstream regulatory pathway of CDK8-CycC (the insulin/mTOR signaling pathway). Our results suggest that CDK8 coordinately regulates lipid biosynthesis by repressing SREBP-activated transcription and developmental timing by activating EcR-activated gene expression during the larval-pupal transition. Interestingly, CDK8 is stabilized by nutrient deprivation and destabilized by feeding, suggesting a novel link between dietary perturbations and the general transcriptional machinery. Furthermore, cdk8 mutants are hyper-sensitive to dietary perturbations, particularly proteins and several specific amino acids, likely through the inhibitory effects of mTOR on CDK8. Further analyses of CDK8 functions and regulation may advance our understanding of how dysregulation of the CDK8 module contributes tumorigenesis and other diseases.-Dr. Jun-yuan Ji
洽詢人員: 劉小姐
洽詢電話: 02-27855696#2061
洽詢信箱: liukchun@gate.sinica.edu.tw