Genome editing is a powerful technique for delineating complex signaling circuitry and for enhancing the functionality of immune cells for immunotherapy. Natural killer (NK) cells are potent immune effectors against cell malignancy, but they are challenging to modify genetically by conventional methods. In this seminar, I will talk about our past effort to establish a robust CRISPR genome editing platform for NK cells, present strategies to overcome DNA toxicity, and future directions to apply our techniques to engineer NK cells for therapeutic applications.-凌嘉鴻博士