精彩研究成果
In vivo tagging and characterization of S-glutathionylated proteins by a chemoenzymatic method

Glutathionylspermidine synthetase (GspS) was introduced into human cells to label endogenous glutathione (GSH) with biotinyl spermine (biotin-spm) for the identification of S-thiolated proteins. The proteins carrying Gspm-biotin can be detected by immunoblotting (A in figure). Enrichment by using the biotin tag enabled subsequent mass-spectrometry-based proteomic analysis (B) for site-specific identification of glutathionylated sites.

論文網站:https://doi.org/10.1002/anie.201200321

作者群:Chiang BY, Chou CC, Hsieh FT, Gao S, Lin JC, Lin SH, Chen TC, Khoo KH*, Lin CH*

更新時間:2012.06.11