中央研究院 生物化學研究所

研究領域與專長

醣生物學與免疫學 (Glycobiology and Immunology)

My role at IBC is to manage the Glycobiology Core Facility (GCF), which supports PIs to conduct glycobiology research by application of the existing technologies, reagents and tools. My expertise also helps facilitate multi-disciplinary collaborations with the initial focus on galectins. The future direction of GCF will be guided by the research projects of IBC PIs. To further promote the utilization of this core, more advanced technologies and platforms will be established per request by PIs.

The Galectin Core Facility is dedicated to services involving:
– Production of glycan-binding proteins (GBPs) and their antibodies; Production and maintenance GBP- and glycan-engineered mammalian cell lines.
– Establishment of analytical platform, using GBPs as glycan sensors (intra- and extra-cellular) and applying GBP engineered reporter cells and GBP array; performance of time-lapse live cell imaging and biochemical analysis of cell signaling.

微生物基因定序與數據分析 (Metagenomic Data analysis)

My personal research interest is to perform microbiome metadata analysis and data visualization. Collaborations with IBC PIs in this emerging research field is also highly welcomed.

學經歷

• 2003 – 2007   博士, 微生物與人類遺傳學系, 美國加州大學爾灣分校
• 2000 – 2002   碩士, 生物藥學研究所, 國立陽明大學
• 1996 – 2000   學士, 生物系, 國立成功大學

• 2022 – 迄今   研究助技師, 醣生物學核心設施, 中研院生化所
• 2017 – 2021   兼任助理教授, 國防醫學院
• 2016 – 2022   專案研究員, 半乳糖凝集素核心設施, 中研院生物醫學研究所
• 2011 – 2015   博士後研究員, 中研院生物醫學研究所
• 2008 – 2010   博士後研究員, 中研院生物醫學研究所

主要著作

Galectin-3 promotes non-canonical inflammasome activation through intracellular binding to lipopolysaccharide glycans. 
Lo TH, Chen HL, Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT* 
Proc Natl Acad Sci USA. (2021)

Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis. 
Chen HL, Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT* 
The Journal of clinical investigation (2021)

Role of skin and gut microbiota in the pathogenesis of psoriasis, an inflammatory skin disease. 
Hsu DK, Fung MA, Chen HL* 
Medicine in Microecology (2020)

MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms. 
Huang JY, Chen HL, Shih C* 
Scientific Rep. (2016)

Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling. 
Chen HL, Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT* 
J. Invest. Dermatol. (2016)

Galectins and neuroinflammation. 
Chen HL, Liao F, Lin TN, Liu FT* 
Advances in neurobiology (2014)

MicroRNA-22 can reduce parathymosin expression in transdifferentiated hepatocytes. 
Chen HL, Huang JY, Chen CM, Chu TH, Shih C* 
PLoS one (2012)

Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis. 
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M* 
Nature communications (2011)

T cell receptor signaling co-regulates multiple Golgi genes to enhance N-glycan branching. 
Chen HL, Li CF, Grigorian A, Tian W, Demetriou M* 
The Journal of biological chemistry (2009)

Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. 
Chen IJ, Chen HL, Demetriou M* 
The Journal of biological chemistry (2007)

著作列表

1. Weng IC, (Chen HL), Lin WH, Liu FT*  (2023-04 accepted)  Glycoconjugate journal  "Sialylation of cell surface glycoconjugates modulates cytosolic galectin-mediated responses upon organelle damage : Minireview."
   
2. Lo TH, (Chen HL), Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT  (2021-07)  Proceedings of the National Academy of Sciences of the United States of America  118(30), e2026246118  "Galectin-3 promotes noncanonical inflammasome activation through intracellular binding to lipopolysaccharide glycans."
   
3. Lei T, Blois SM, Freitag N, Bergmann M, Bhushan S, Wahle E, Huang AC, (Chen HL), Hartmann MF, Wudy SA, Liu FT, Meinhardt A, Fijak M  (2021-04)  Cellular and molecular life sciences  78(7), 3621-3635  "Targeted disruption of galectin 3 in mice delays the first wave of spermatogenesis and increases germ cell apoptosis."
   
4. Lo YH, Li CS, (Chen HL), Chiang CY, Huang CC, Tu TJ, Lo TH, Choy DF, Arron JR, Chen HY, Liu FT  (2021-03)  The Journal of investigative dermatology  141(3), 503-511.e9  "Galectin-8 Is Upregulated in Keratinocytes by IL-17A and Promotes Proliferation by Regulating Mitosis in Psoriasis."
   
5. (Chen HL), Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT  (2021-01)  The Journal of clinical investigation  131(1), 130740  "Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis."
   
6. Hsu DK, Fung MA, (Chen HL)*  (2020-06)  Medicine in Microecology  4, 100016  "Role of skin and gut microbiota in the pathogenesis of psoriasis, an inflammatory skin disease."
   
7. Hong MH, Lin WH, Weng IC, Hung YH, (Chen HL), Chen HY, Chen P, Lin CH, Yang WY, Liu FT  (2020)  Glycobiology  30(1), 46-48  "Intracellular galectins control cellular responses commensurate with cell surface carbohydrate composition."
   
8. Siew JJ, Chen HM, Chen HY, (Chen HL), Chen CM, Soong BW, Wu YR, Chang CP, Chan YC, Lin CH, Liu FT, Chern Y  (2019-08)  Nature communications  10(1), 3473  "Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington\'s disease."
   
9. Weng IC, (Chen HL), Lo TH, Lin WH, Chen HY, Hsu DK, Liu FT  (2018-06)  Glycobiology  28(6), 392-405  "Cytosolic galectin-3 and -8 regulate antibacterial autophagy through differential recognition of host glycans on damaged phagosomes."
   
10. Huang WC, (Chen HL), Chen HY, Peng KP, Lee Y, Huang LM, Chang LY, Liu FT  (2016-12)  PloS one  11(12), e0168627  "Galectin-3 and Its Genetic Variation rs4644 Modulate Enterovirus 71 Infection."
    
11. Huang JY, (Chen HL), Shih C  (2016-10)  Scientific reports  6, 34740  "MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms."
    
12. (Chen HL), Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT  (2016-01)  The Journal of investigative dermatology  136(1), 182-191  "Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling."
    
13. Huang JY, Chou SF, Lee JW, (Chen HL), Chen CM, Tao MH, Shih C  (2015-01)  RNA  21(3), 385-400  "MicroRNA-130a can inhibit hepatitis B virus replication via targeting PGC1alpha and PPARgamma."
    
14. (Chen HL), Huang JY, Chen CM, Chu TH, Shih C  (2012-04)  PloS one  7(4), e34116  "MicroRNA-22 can reduce parathymosin expression in transdifferentiated hepatocytes."
    
15. Mkhikian H, Grigorian A, Li CF, (Chen HL), Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M  (2011-05)  Nature communications  2, 334  "Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis."
    
16. (Chen HL), Li CF, Grigorian A, Tian W, Demetriou M  (2009-11)  The Journal of biological chemistry  284(47), 32454-32461  "T cell receptor signaling co-regulates multiple Golgi genes to enhance N-glycan branching."
    
17. Chen IJ, (Chen HL), Demetriou M  (2007-11)  The Journal of biological chemistry  282(48), 35361-35372  "Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling."
    
18. (Chen HL), Lu CY, Hsu YH, Lin JJ  (2004-01)  Biochemical and biophysical research communications  313(3), 576-586  "Chromosome positional effects of gene expressions after cellular senescence."
    
19. Teng SC, Epstein C, Tsai YL, Cheng HW, (Chen HL), Lin JJ  (2002-03)  Biochemical and biophysical research communications  291(3), 714-721  "Induction of global stress response in Saccharomyces cerevisiae cells lacking telomerase."