中央研究院 生物化學研究所

研究領域與專長

Our Interests

My lab is using electron cryo-microscopy (cryo-EM) to determine the atomic structures of macromolecules.

Molecular Virology and Microbiology

A special focus lies on infectious diseases. I have previously worked on the structures of human viruses, including HIV, ebola, polio, papilloma, polyoma and seneca valley virus, as well as some large insect viruses and bacteriophages. Another branch of research is focused on helical assemblies – in particular, bacterial and archaeal flagella that enable cellular motility. With significant experience in image processing methods, we have succeeded in determining the structures of objects with unconventional (e.g. superhelical) or no symmetry. The underlying goal in all projects is to link structure and function to discover mechanisms of pathogenicity, with the hope to contribute towards novel treatments of the related diseases.

A wider scale of view

At Academia Sinica, we will develop unique labels usable for light- and cryo-EM. We will combine fluorescence light microscopy, cellular tomography and single particle cryo-EM to characterize viral pathways in living cells.

學經歷

• 2005   PhD in Biophysical and Structural Biology, Brandeis University, MA, USA
• 1998   Mag.Pharm. (MPharm) in Pharmacy, University of Innsbruck, Austria

• 2023 – present   Joint Appointment Research Fellow, Institute of Biological Chemistry, Academia Sinica
• 2021 – present   Professor (tenured), Okinawa Institute of Technology (Okinawa, Japan), Head of the Molecular Cryo-Electron Microscopy Unit
• 2021 – 2022   Joint Appointment Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
• 2019 – 2021   Associate Professor, Okinawa Institute of Technology (Okinawa, Japan), Head of the Molecular Cryo-Electron Microscopy Unit
• 2011 – 2018   Assistant Professor, Okinawa Institute of Technology (Okinawa, Japan), Head of the Molecular Cryo-Electron Microscopy Unit
• 2009 – 2011   Joint Appointment, Hogle Lab and Harrison Lab, Harvard Medical School
• 2005 – 2011    Postdoctoral Fellow, Harrison Lab at Harvard Medical School and Children’s Hospital, Boston, USA
• 1999 – 2005   Graduate Research Fellow, PhD Program in Biophysics under Prof. N. Grigorieff, Brandeis University, MA, USA

主要著作

COVID-19 serological survey using micro blood sampling. 
Matthews MM, Kim TG, Shibata S, Shibata N, Butcher C, Hyun J, Kim KY, Robb T, Jheng SS, Narita M, Mori T, Collins M, Wolf M*. 
MedRxiv (2020)

Structure of polymerized Type V pilin reveals assembly mechanism involving protease-mediated strand exchange. 
Shibata S, Shoji M, Okada K, Matsunami H, Matthews MM, Imada K*, Nakayama K*, Wolf M*. 
Nature Microbiology (2020)

Torque transmission mechanism of the curved bacterial flagellar hook revealed by cryo-EM. 
Shibata S, Matsunami H, Aizawa SI*, Wolf M*. 
Nature Structural & Molecular Biology (2019)

High-resolution archaellum structure reveals a conserved metal binding site. 
Meshcheryakov VA, Shibata S, Schreiber MT, Villar-Briones A, Jarrell KF, Aizawa SI*, Wolf M*. 
EMBO Reports (2019)

Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex at 3.6 Å resolution. 
Sugita Y, Matsunami H, Kawaoka Y, Noda T, Wolf M*. 
Nature (2018)

Structural basis for Anthrax Toxin Receptor 1 recognition by Seneca Valley Virus. 
Jayawardena N, Burga LN, Easingwood RA, Takizawa Y, Wolf M*, Bostina M*. 
Proc. Natl. Acad. Sci. (2018)

Structural Basis of Heterochromatin Formation by Human HP1. 
Machida S, Takizawa Y, Ishimaru M, Sugita Y, Sekine S, Nakayama JI, Wolf M*, Kurumizaka H*. 
Molecular Cell (2018)

Low-dose electron energy-loss spectroscopy using electron counting direct detectors. 
Maigné A, Wolf M*. 
Microscopy (Oxford, UK) (2018)

Subunit interactions in bovine papillomavirus. 
Wolf M, Garcea RL, Grigorieff N, Harrison SC. 
Proc. Natl. Acad. Sci. (2010)

Ewald sphere correction for single-particle electron microscopy. 
Wolf M, DeRosier DJ, Grigorieff N. 
Ultramicroscopy (2006)

著作列表