中央研究院 生物化學研究所

It has now become possible to reconstitute various biological systems in vitro, which exhibit features of the living cells, by assembling defined molecules. Using cell-sized liposomes and a reconstituted cell-free protein synthesis system, we have constructed an artificial cell from approximately 100 different defined molecules, which can synthesize protein of interest inside the cell-sized liposome. By using this in liposome protein synthesis system, we have been synthesizing various membrane proteins that enable the characterization of membrane proteins. In addition, in liposome protein synthesis system can be used for the directed evolution of membrane proteins entirely. Here, I will present the construction of in liposome membrane protein synthesis system and its application. -Dr. Tomoaki Matsuura