Latest Publications

2024

Jul
02
A metabolic inhibitor blocks cellular fucosylation and enables production of afucosylated antibodies
Proc Natl Acad Sci U S A. 2024 Jul 2; 121(27):e2314026121. Epub 2024 Jun 25.
Kay-Hooi Khoo

The fucosylation of glycoproteins regulates diverse physiological processes. Inhibitors that can control cellular levels of prot

 
Jul 02 2024
Jul
01
Transition transferases prime bacterial capsule polymerization
Nat Chem Biol. 2024 Jul 1. Online ahead of print.
Todd L Lowary

Capsules are long-chain carbohydrate polymers that envelop the surfaces of many bacteria, protecting them from host immune respo

 
Jul 01 2024
Jul
01

Klebsiella pneumoniae provides influential prototypes for lipopolysaccharide O antigen (OPS) biosynthesis in Gram-negative bacte

 
Jul 01 2024
Jun
27

Peptidoglycan (PG) sacculi surround the cytoplasmic membrane, maintaining cell integrity by withstanding internal turgor pressur

 
Jun 27 2024
Jun
15
Dual dimeric interactions in the nucleic acid-binding protein Sac10b lead to multiple bridging of double-stranded DNA
Heliyon. 2024 May 23; 10(11):e31630. eCollection 2024 Jun 15.
Yuan-Chih Chang

Nucleoid-associated proteins play a crucial role in the compaction and regulation of genetic material across organisms. The Sac1

 
Jun 15 2024
Jun
12
Exploring a De Novo Route to Bradyrhizose: Synthesis and Isomeric Equilibrium of Bradyrhizose Diastereomers
Chemistry. 2024 Jun 12; 30(33):e202400886. Epub 2024 May 3.
Todd L Lowary

A de novo asymmetric strategy for the synthesis of d-bradyrhizose diastereomers from an achiral ketoenolester precursor is descr

 
Jun 12 2024
Jun
04

The causative pathogen of COVID-19, severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), utilizes the receptor-binding

 
Jun 04 2024
Jun
01

Visualizing liposome release profiles in small animals is important for evaluating the pharmacokinetic influence of vesicles. En

 
Jun 01 2024
Jun
01

Ovarian carcinoma is usually diagnosed at an advanced stage with peritoneal dissemination and/or lymph node metastasis, and the

 
Jun 01 2024
Jun
01

Prions, the misfolding form of prion proteins, are contagious proteinaceous macromolecules. Recent studies have shown that infec

 
Jun 01 2024
Jun
01

Alcohol-associated liver disease (ALD) is a leading factor of liver-related death worldwide. ALD has various manifestations that

 
Jun 01 2024
May
22
Functional and structural investigation of a broadly neutralizing SARS-CoV-2 antibody
JCI Insight. 2024 May 22; 9(10):e179726.
Shang-Te Danny Hsu, Takashi Angata, Yuan-Chih Chang

Since its emergence, SARS-CoV-2 has been continuously evolving, hampering the effectiveness of current vaccines against COVID-19

 
May 22 2024
May
21
Three-component systems represent a common pathway for extracytoplasmic addition of pentofuranose sugars into bacterial glycans
Proc Natl Acad Sci U S A. 2024 May 21; 121(21):e2402554121. Epub 2024 May 15.
Todd L Lowary

Cell surface glycans are major drivers of antigenic diversity in bacteria. The biochemistry and molecular biology underpinning t

 
May 21 2024
May
13
Non-Heme Iron Enzymes Catalyze Heterobicyclic and Spirocyclic Isoquinolone Core Formation in Piperazine Alkaloid Biosynthesis
Angew Chem Int Ed Engl. 2024 May 13; 63(20):e202401324. Epub 2024 Apr 9.
Hsiao-Ching Lin, Po-Huang Liang

We report the discovery and biosynthesis of new piperazine alkaloids-arizonamides, and their derived compounds-arizolidines, fea

 
May 13 2024
May
08

Formation of programmed DNA double-strand breaks is essential for initiating meiotic recombination. Genetic studies on Arabidops

 
May 08 2024
May
08
Structural basis and synergism of ATP and Na+ activation in bacterial K+ uptake system KtrAB
Nature Communications. 2024 May 8; 15(1):3850.
Ming-Daw Tsai

The K+ uptake system KtrAB is essential for bacterial survival in low K+ environments. The activity of KtrAB is regulated by nuc

 
May 08 2024
May
01
Structural Insight into the ZFAND1-p97 Interaction Involved in Stress Granule Clearance
Journal of Biological Chemistry. 2024 May; 300(5):107230. Epub 2024 Mar 25.
Shang-Te Danny Hsu

Arsenite-induced stress granule (SG) formation can be cleared by the ubiquitin-proteasome system aided by the ATP-dependent unfo

 
May 01 2024
Apr
29

Background: Helicobacter pylori, the main cause of various gastric diseases, infects approximately half of the human population.

 
Apr 29 2024
Apr
23
Lipoarabinomannan modification as a source of phenotypic heterogeneity in host-adapted Mycobacterium abscessus isolates
Proc Natl Acad Sci U S A. 2024 Apr 23; 121(17):e2403206121. Epub 2024 Apr 17.
Todd L. Lowary

Mycobacterium abscessus is increasingly recognized as the causative agent of chronic pulmonary infections in humans. One of the

 
Apr 23 2024
Apr
19
Bifurcated assembly pathway and dual function of a Lon-like protease revealed by cryo-EM Analysis
Fundamental Research. 2024 Apr 19. Online ahead of print.
Chung-I Chang

The Lon proteases are evolutionarily conserved oligomeric hydrolases characterized by a built-in ATPase module. Here, we report

 
Apr 19 2024
Apr
03

Viral RNA-dependent RNA polymerase (RdRp), a highly conserved molecule in RNA viruses, has recently emerged as a promising drug

 
Apr 03 2024
Apr
01

Deinagkistrodon acutus is a medically important pitviper inhabiting mainly South China and Taiwan. The hemorrhagic effects of it

 
Apr 01 2024
Mar
25

The use of pharmacological chaperones (PCs) to stabilize specific enzymes and impart a therapeutic benefit is an emerging strate

 
Mar 25 2024
Mar
19
Expression of low-sulfated keratan sulfate in non-mucinous ovarian carcinoma
Glycobiology. 2024 Mar 19; 34(1):cwad056.
Kay-Hooi Khoo

Keratan sulfate glycosaminoglycan is composed of repeating N-acetyllactosamine (LacNAc) disaccharide units consisting of galacto

 
Mar 19 2024
Mar
13
Klebsiella pneumoniae K2 capsular polysaccharide degradation by a bacteriophage depolymerase does not require trimer formation
mBio. 2024 Mar 13; 15(3):e0351923. Epub 2024 Feb 13.
Shih-Hsiung Wu, Kai-Fa Huang

K2-capsular Klebsiella pneumoniae is a hypervirulent pathogen that causes fatal infections. Here, we describe a phage tailspike

 
Mar 13 2024
Mar
07

Pseudaminic acid (Pse) on pathogenic bacteria exopolysaccharide engages with the sialic acid-binding immunoglobulin-type lectin

 
Mar 07 2024
Feb
29
Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries
Cell. 2024 Feb 29; 187(5):1296-1311.e26.
Shang-Te Danny Hsu, Kay-Hooi Khoo

Most membrane proteins are modified by covalent addition of complex sugars through N- and O-glycosylation. Unlike proteins, glyc

 
Feb 29 2024
Feb
28
A unified view on enzyme catalysis by cryo-EM study of a DNA topoisomerase
Commun Chem. 2024 Feb 28; 7(1):45.
Ming-Daw Tsai, Wen-Jin Wu

The theories for substrate recognition in enzyme catalysis have evolved from lock-key to induced fit, then conformational select

 
Feb 28 2024
Feb
23
Sulfated disaccharide protects membrane and DNA damages from arginine-rich dipeptide repeats in ALS
Sci Adv. 2024 Feb 23; 10(8):eadj0347. Epub 2024 Feb 23.
Chi-Kuang Yao

Hexanucleotide repeat expansion in C9ORF72 (C9) is the most prevalent mutation among amyotrophic lateral sclerosis (ALS) patient

 
Feb 23 2024
Feb
15
Altered protein dynamics and a more reactive catalytic cysteine in a neurodegeneration-associated UCHL1 mutant
J Mol Biol. 2024 Feb 15; 436(4):168438. Epub 2024 Jan 5.
Shang-Te Danny Hsu

A mutant of ubiquitin C-terminal hydrolase L1 (UCHL1) detected in early-onset neurodegenerative patients, UCHL1R178Q, showed hig

 
Feb 15 2024
Feb
14

The type VI secretion system (T6SS) assembles into a contractile nanomachine to inject effectors across bacterial membranes for

 
Feb 14 2024
Feb
12
Tying a true topological protein knot by cyclization
Biochem Biophys Res Commun. 2024 Feb 12; 696:149470. Epub 2024 Jan 12.
Shang-Te Danny Hsu

Knotted proteins are fascinating to biophysicists because of their robust ability to fold into intricately defined three-dimensi

 
Feb 12 2024
Feb
09

Campylobacters are important causes of gastrointestinal illness and the capsular polysaccharides (CPS) they produce are key viru

 
Feb 09 2024
Feb
03

The epidermal growth factor receptor (EGFR) plays important roles in multiple cellular events, including growth, differentiation

 
Feb 03 2024
Feb
01

Hirudins, natural sulfo(glyco)proteins, are clinical anticoagulants that directly inhibit thrombin, a key coagulation factor. Th

 
Feb 01 2024
Feb
01

The canine anti-TNF-α monoclonal antibody is a potential therapeutic option for treating canine arthritis. The current treatment

 
Feb 01 2024
Jan
27

Genome integrity is threatened by solar ultraviolet (UV) light, which reacts with adjacent DNA pyrimidine bases to produce undes

 
Jan 27 2024
Jan
01

Background: Increasing evidence supports that antibodies can protect against active tuberculosis (TB) but knowledge of potential

 
Jan 01 2024
Jan
01
Protein tyrosine phosphatase 1B is a regulator of alpha-actinin4 in the glomerular podocyte
Biochim Biophys Acta Mol Cell Res. 2024 Jan; 1871(1):119590. Epub 2023 Sep 18.
Tzu-Ching Meng

Glomerular podocytes are instrumental for the barrier function of the kidney, and podocyte injury contributes to proteinuria and

 
Jan 01 2024
Jan
01
Structure, dynamics and stability of the smallest and most complex 71 protein knot
Journal of Biological Chemistry. 2024 Jan; 300(1):105553.
Shang-Te Danny Hsu, Kai-Fa Huang

Proteins can spontaneously tie a variety of intricate topological knots through twisting and threading of the polypeptide chains

 
Jan 01 2024

2023

Dec
21

The cyclic GMP-AMP synthase (cGAS)/stimulator of interferon gene (STING) signaling pathway plays a critical protective role agai

 
Dec 21 2023
Dec
15
What strengthens Protein-Protein Interactions: Analysis and Applications of Residue Correlation Networks
J Mol Biol. 2023 Dec 15; 435(24):168337. Epub 2023 Oct 31.
Kuen-Phon Wu

Identifying residues critical to protein-protein binding and efficient design of stable and specific protein binders are challen

 
Dec 15 2023
Dec
14
Characterization and catalytic investigation of fungal single-module nonribosomal peptide synthetase in terpene-amino acid meroterpenoid biosynthesis
J Ind Microbiol Biotechnol. 2023 Dec 14; 50(1):kuad043.
Hsiao-Ching Lin, Chun-Hung Lin, Zhijay Tu

Hybrid natural products are compounds that originate from diverse biosynthetic pathways and undergo a conjugation process, which

 
Dec 14 2023
Dec
11

The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is clos

 
Dec 11 2023
Dec
07
An integrative approach unveils a distal encounter site for rPTPε and phospho-Src complex formation
Structure. 2023 Dec 7; 31(12):1567-1577.e5. Epub 2023 Oct 3.
Meng-Chiao Ho, Guang-Chao Chen

The structure determination of protein tyrosine phosphatase (PTP): phospho-protein complexes, which is essential to understand h

 
Dec 07 2023
Dec
04
A structure of the relict phycobilisome from a thylakoid-free cyanobacterium
Nature Communications. 2023 Dec 4; 14(1):8009.
Meng-Chiao Ho, Ming-Daw Tsai

Phycobilisomes (PBS) are antenna megacomplexes that transfer energy to photosystems II and I in thylakoids. PBS likely evolved f

 
Dec 04 2023
Dec
01
Visualizing the DNA repair process by a photolyase at atomic resolution
Science. 2023 Dec; 382(6674):eadd7795. Epub 2023 Dec 1.
Ming-Daw Tsai, Manuel Maestre-Reyna, Wen-Jin Wu, Kai-Fa Huang

Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the str

 
Dec 01 2023
Dec
01

Background/aim: PHD and RING finger domain-containing protein 1 (PHRF1) ubiquitinates TGIP (TG-interacting protein) and redistri

 
Dec 01 2023
Dec
01

Eukaryotic dehydrodolichyl diphosphate synthases (DHDDSs), cis-prenyltransferases (cis-PTs) synthesizing precursors of dolichols

 
Dec 01 2023
Nov
30

Keeping replication fork stable is essential for safeguarding genome integrity; hence, its protection is highly regulated. The C

 
Nov 30 2023
Nov
27
RAD51 paralogs synergize with RAD51 to protect reversed forks from cellular nucleases
Nucleic Acids Res. 2023 Nov 27; 51(21):11717-11731.
Peter Chi, Hsin-Yung Yen

Fork reversal is a conserved mechanism to prevent stalled replication forks from collapsing. Formation and protection of reverse

 
Nov 27 2023
Nov
21

Homologous recombination (HR)-mediated DNA repair is a prerequisite for maintaining genome stability. Cancer cells displaying HR

 
Nov 21 2023
Nov
16
PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly
Mol Cell. 2023 Nov 16; 83(22):4123-4140.e12. Epub 2023 Oct 16.
Ruey-Hwa Chen, Meng-Chiao Ho, Kuen-Phon Wu

Purinosomes serve as metabolons to enhance de novo purine synthesis (DNPS) efficiency through compartmentalizing DNPS enzymes du

 
Nov 16 2023
Nov
16
Terpene Synthases in the Biosynthesis of Drimane-Type Sesquiterpenes across Diverse Organisms
Chembiochem. 2023 Nov 16; 24(22):e202300518.
Hsiao-Ching Lin

Drimane-type sesquiterpenes (DTSs) are significant terpenoid natural products characterized by their unique C15 bicyclic skeleto

 
Nov 16 2023
Nov
13
A 5+1 assemble-to-activate mechanism of the Lon proteolytic machine
Nat Commun. 2023 Nov 13;14(1):7340.
Chung-I Chang, Meng-Ru Ho

Many AAA+ (ATPases associated with diverse cellular activities) proteins function as protein or DNA remodelers by threading the

 
Nov 13 2023
Nov
01

A class of 1-(4-(arylethylenylcarbonyl)phenyl)-4-carboxy-2-pyrrolidinones were designed and synthesized via Michael addition, cy

 
Nov 01 2023
Nov
01
Development of AAV-delivered broadly neutralizing anti-human ACE2 antibodies against SARS-CoV-2 variants
Mol Ther. 2023 Nov 1; 31(11):3322-3336. Epub 2023 Sep 9.
Shang-Te Danny Hsu, Yuan-Chih Chang

The ongoing evolution of SARS-CoV-2, resulting in the emergence of new variants that are resistant to existing vaccines and ther

 
Nov 01 2023
Oct
31

The peptidoglycan layer is a defining characteristic of bacterial cells, providing them with structural support and osmotic prot

 
Oct 31 2023
Oct
27

Translational readthrough of UGA stop codons by selenocysteine-specific tRNA (tRNASec) enables the synthesis of selenoproteins.

 
Oct 27 2023
Oct
23

A better understanding of the epitopes most relevant for antibody-mediated protection against tuberculosis (TB) remains a major

 
Oct 23 2023
Oct
15

Complete coverage of all N-glycosylation sites on the SARS-CoV2 spike protein would require the use of multiple proteases in add

 
Oct 15 2023
Oct
13
Structural basis of transcriptional activation by the OmpR/PhoB-family response regulator PmrA
Nucleic Acids Research. 2023 Oct 13; 51(18):10049-10058.
Kuen-Phon Wu

PmrA, an OmpR/PhoB-family response regulator, triggers gene transcription responsible for polymyxin resistance in bacteria by re

 
Oct 13 2023
Oct
13
Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids
Frontiers in Genetics. 2023 Oct 13; 14:1277489.
Yane-Shih Wang, Ming-Daw Tsai

Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of phys

 
Oct 13 2023
Oct
11
PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target
Cell Death Dis. 2023 Oct 11; 14(10):671.
Ruey-Hwa Chen, Kuen-Phon Wu, Shu-Yu Lin

Aberrant overexpression or activation of EGFR drives the development of non-small cell lung cancer (NSCLC) and acquired resistan

 
Oct 11 2023
Sep
23
Structural basis for calcium-stimulating pore formation of Vibrio α-hemolysin
Nat Commun. 2023 Sep 23; 14(1):5946.
Meng-Chiao Ho

Vibrio α-hemolysins (αHLs) are β-pore-forming toxins secreted by Vibrio pathogens, crucial for the facilitation of bacterial inf

 
Sep 23 2023
Sep
19
Nanoscale details of mitochondrial constriction revealed by cryo-electron tomography
Biophys J. 2023 Sep 19; 122(18):3768-3782. Epub 2023 Aug 1.
Wei Yuan Yang

Mitochondria adapt to changing cellular environments, stress stimuli, and metabolic demands through dramatic morphological remod

 
Sep 19 2023
Sep
10
Elucidation of the folding pathway of a circular permutant of topologically knotted YbeA by tryptophan substitutions
Biochem Biophys Res Commun. 2023 Sep 10; 672:81-88. Epub 2023 Jun 14.
Shang-Te Danny Hsu

CP74 is an engineered circular permutant of a deep trefoil knotted SpoU-TrmD (SPOUT) RNA methyl transferase protein YbeA from E.

 
Sep 10 2023
Sep
06
Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography
Nature Communications. 2023 Sep 6; 14:5464.
Rita P.-Y. Chen, Meng-Chiao Ho

The abuse of antibiotics has led to the emergence of multidrug-resistant microbial pathogens, presenting a pressing challenge in

 
Sep 06 2023
Sep
04

Membrane proteins are challenging to analyze by native mass spectrometry (MS) as their hydrophobic nature typically requires sta

 
Sep 04 2023
Aug
28

Cellular glycosylation is characterized by chemical complexity and heterogeneity, which is challenging to reproduce syntheticall

 
Aug 28 2023
Aug
22
The deubiquitinase Leon/USP5 interacts with Atg1/ULK1 and antagonizes autophagy
Cell Death Dis. 2023 Aug 22; 14(8):540.
Guang-Chao Chen

Accumulating evidence has shown that the quality of proteins must be tightly monitored and controlled to maintain cellular prote

 
Aug 22 2023
Aug
18
Robust Design of Effective Allosteric Activators for Rsp5 E3 Ligase Using the Machine Learning Tool ProteinMPNN
ACS Synthetic Biology. 2023 Aug 18; 12(8):2310-2319. Epub 2023 Aug 9.
Kuen-Phon Wu, Shang-Te Danny Hsu, Meng-Ru Ho

We used the deep learning tool ProteinMPNN to redesign ubiquitin (Ub) as a specific and functionally stimulating/enhancing binde

 
Aug 18 2023
Aug
17

ATP-dependent RAD51 recombinases play an essential role in eukaryotic homologous recombination by catalyzing a four-step process

 
Aug 17 2023
Aug
04
PHRF1 promotes the class switch recombination of IgA in CH12F3-2A cells
PLoS One. 2023 Aug 4; 18(8):e0285159. eCollection 2023.
Mau-Sun Chang

PHRF1 is an E3 ligase that promotes TGF-β signaling by ubiquitinating a homeodomain repressor TG-interacting factor (TGIF). The

 
Aug 04 2023
Aug
01

The Gram-negative anaerobe, Porphyromonas gingivalis, is known to be a pathogen associated with chronic periodontitis. P. gingiv

 
Aug 01 2023
Jul
24

The intracellular phosphatase domain of the receptor-type protein tyrosine phosphatase alpha (PTPRA) is known to regulate variou

 
Jul 24 2023
Jul
20
An ATP-sensitive phosphoketolase regulates carbon fixation in cyanobacteria
Nat Metab. 2023 Jul; 5(7):1111-1126. Epub 2023 Jun 22.
James C Liao, Ming-Daw Tsai, Meng-Chiao Ho, Wen-Jin Wu

Regulation of CO2 fixation in cyanobacteria is important both for the organism and global carbon balance. Here we show that phos

 
Jul 20 2023
Jul
18
Identification of a second glycoform of the clinically prevalent O1 antigen from Klebsiella pneumoniae
Proc Natl Acad Sci U S A. 2023 Jul 18; 120(29):e2301302120. Epub 2023 Jul 10.
Todd L. Lowary

Carbapenemase and extended β-lactamase-producing Klebsiella pneumoniae isolates represent a major health threat, stimulating inc

 
Jul 18 2023
Jul
11

Most rice (Oryza sativa) cultivars cannot survive under prolonged submergence. However, some O. sativa ssp. indica cultivars, su

 
Jul 11 2023
Jul
07
Interrogation of human microglial phagocytosis by CRISPR genome editing
Front Immunol. 2023 Jul 7; 14:1169725.
Steven Lin

Background: Microglia are an integral part of central nervous system, but our understanding of microglial biology is limited due

 
Jul 07 2023
Jul
07

Great effort has been devoted to discovering the basis of A3G-Vif interaction, the key event of HIV's counteraction mechanism to

 
Jul 07 2023
Jul
01

The TFIIF-like Rpc53/Rpc37 heterodimer of RNA polymerase (pol) III is involved in various stages of transcription. The C-termina

 
Jul 01 2023
Jun
22

Trimeresurus gracilis is an endemic alpine pitviper in Taiwan with controversial phylogeny, and its venom proteome remains unkno

 
Jun 22 2023
Jun
06
Acetylation-Mimic Mutation of TRIM28-Lys304 to Gln Attenuates the Interaction with KRAB-Zinc-Finger Proteins and Affects Gene Expression in Leukemic K562 Cells
International Journal of Molecular Sciences. 2023 Jun 6; 24(12):9830.
Ching-Jin Chang, Steven Lin, Shu-Yu Lin, Sheng-Wei Lin

TRIM28/KAP1/TIF1β is a crucial epigenetic modifier. Genetic ablation of trim28 is embryonic lethal, although RNAi-mediated knock

 
Jun 06 2023
Jun
02

Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including

 
Jun 02 2023
Jun
01
Cryo-EM reveals the structure and dynamics of a 723-residue malate synthase G
Journal of Structural Biology. 2023 Jun; 215(2):107958. Epub 2023 Mar 28.
Kuen-Phon Wu, Meng-Ru Ho

Determination of sub-100 kilodalton (kDa) structures by cryo-electron microscopy (EM) is a longstanding but not straightforward

 
Jun 01 2023
May
26

Activation of tumor-intrinsic innate immunity has been a major strategy for improving immunotherapy. Previously, we reported an

 
May 26 2023
May
25
A phosphate-sensing organelle regulates phosphate and tissue homeostasis
Nature. 2023 May; 617(7962):798-806. Epub 2023 May 3.
Jui-Hsia Weng

Inorganic phosphate (Pi) is one of the essential molecules for life. However, little is known about intracellular Pi metabolism

 
May 25 2023
May
22

Human mitochondrial NAD(P)+-dependent malic enzyme (ME2) is well-known for its role in cell metabolism, which may be involved in

 
May 22 2023
May
22

The long non-coding telomeric RNA transcript TERRA, in the form of an RNA-DNA duplex, regulates telomere recombination. In a scr

 
May 22 2023
May
17
Cholesterol twists the transmembrane Di-Gly region of amyloid-precursor protein
PNAS Nexus. 2023 May 17; 2(5):pgad162. eCollection 2023 May.
Rita P.-Y. Chen

Nearly 95% of Alzheimer's disease (AD) occurs sporadically without genetic linkage. Aging, hypertension, high cholesterol conten

 
May 17 2023
May
12
Monitoring Stub1-Mediated Pexophagy
J Vis Exp. 2023 May 12; (195):e65010.
Wei Yuan Yang

Mammalian cells can turn over peroxisomes through Stub1-mediated pexophagy. The pathway potentially permits cellular control of

 
May 12 2023
May
01

KpsC is a dual-module glycosyltransferase (GT) essential for "group 2" capsular polysaccharide biosynthesis in Escherichia coli

 
May 01 2023
Apr
22

Immunomodulatory Siglecs are controlled by their glycoprotein and glycolipid ligands. Siglec-glycolipid interactions are often s

 
Apr 22 2023
Apr
19

White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, wit

 
Apr 19 2023
Apr
05

The synthesis of the cell-wall peptidoglycan during bacterial cell division is mediated by a multiprotein machine, called the di

 
Apr 05 2023
Apr
01

Enterocloster bolteae (formerly known as Clostridium bolteae) is a gastro-intestinal pathogenic bacterium often detected in the

 
Apr 01 2023
Mar
29

Extracellular vesicles (EVs) are released by cells to mediate intercellular communication under pathological and physiological c

 
Mar 29 2023
Mar
20

Since the discovery of protein tyrosine phosphorylation as one of the critical post-translational modifications, it has been wel

 
Mar 20 2023
Mar
15

The cDNAs encoding the Zn+2-metalloproteases (SVMPs) of Trimeresurus gracilis (abbreviated as Tgc), a pitviper endemic to Taiwan

 
Mar 15 2023
Feb
27
Genome mining of cryptic bisabolenes that were biosynthesized by intramembrane terpene synthases from Antrodia cinnamomea
Philos Trans R Soc Lond B Biol Sci. 2023 Feb 27; 378(1871):20220033. Epub 2023 Jan 11.
Hsiao-Ching Lin

Terpenoids represent the largest structural family of natural products (NPs) and have various applications in the pharmaceutical

 
Feb 27 2023
Feb
21
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression
Proc Natl Acad Sci U S A. 2023 Feb 21; 120(8):e2219758120. Epub 2023 Feb 14.
Yane-Shih Wang

Synthetic biology tools for regulating gene expression have many useful biotechnology and therapeutic applications. Most tools d

 
Feb 21 2023
Feb
20
The Biosynthetic Gene Cluster of Mushroom-Derived Antrocin Encodes Two Dual Functional Haloacid Dehalogenase-Like Terpene Cyclases
Angew Chem Int Ed Engl. 2023 Feb 20; 62(9):e202215566.
Hsiao-Ching Lin, Shih-Hsiung Wu

(-)-Antrocin (1), produced by the medicinal mushroom, Antrodia cinnamomea, is a potent anti-proliferative compound. The biosynth

 
Feb 20 2023
Feb
17
SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron
iScience. 2023 Feb 17; 26(2):105995. Epub 2023 Jan 16.
James C. Liao

The coronavirus nucleocapsid (N) protein is known to bind to nucleic acids and facilitate viral genome encapsulation. Here we re

 
Feb 17 2023
Feb
16

Acinetobacter baumannii is a nosocomial pathogen that can be resistant to antibiotics by rapidly modulating its anti-drug mechan

 
Feb 16 2023
Feb
01

Understanding the structural diversity of honeybee-infecting viruses is critical to maintain pollinator health and manage the sp

 
Feb 01 2023
Jan
31
Cytosolic galectin-4 enchains bacteria, restricts their motility, and promotes inflammasome activation in intestinal epithelial cells
Proc Natl Acad Sci U S A. 2023 Jan 31; 120(5):e2207091120. Epub 2023 Jan 23.
Chun-Hung Lin

Galectin-4, a member of the galectin family of animal glycan-binding proteins (GBPs), is specifically expressed in gastrointesti

 
Jan 31 2023
Jan
21

Patients with mutations of WDR4, a substrate adaptor of the CUL4 E3 ligase complex, develop cerebellar atrophy and gait phenotyp

 
Jan 21 2023
Jan
03
Temporal dynamics of charge buildup in cryo-electron microscopy
J Struct Biol X . 2023; 7:100081.
Matthias Wolf

It is well known that insulating samples can accumulate electric charges from exposure to an electron beam. How the accumulation

 
Jan 03 2023
Jan
02
Climate change challenges, plant science solutions
Plant Cell. 2023 Jan 2; 35(1):24-66.
James C. Liao

Climate change is a defining challenge of the 21st century, and this decade is a critical time for action to mitigate the worst

 
Jan 02 2023
Jan
02

We developed a versatile asymmetric strategy to synthesize different classes of sulfoglycolipids (SGLs) from Mycobacterium tuber

 
Jan 02 2023
Jan
01

Reactive oxygen species (ROS), released as byproducts of mitochondrial metabolism or as products of NADPH oxidases and other pro

 
Jan 01 2023
Jan
01
Cross-Seeding Assay in the Investigation of the Amyloid Core of Prion Fibrils
Methods Mol Biol. 2023; 2551:633-647.
Rita P.-Y. Chen

Amyloidogenesis, self-propagation of protein or peptide monomers to amyloid fibrils, has been linked to incurable pathogenesis o

 
Jan 01 2023

2022

Dec
13

Replication fork reversal which restrains DNA replication progression is an important protective mechanism in response to replic

 
Dec 13 2022
Dec
06
Expeditious and Stereoselective Synthesis of (1S)-Spiro[pyran-4-quinolines]
European Journal of Organic Chemistry. 2022 Dec 6; 2022(45):e202201116.
Chun-Hung Lin

Spiro[pyran-4-quinolines] were synthesized in two steps from exo-glycals. The first step involved the double additions of anilin

 
Dec 06 2022
Dec
06
Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor
Nat Chem. 2022 Dec; 14(12):1375-1382. Epub 2022 Nov 10.
Hsin-Yung Yen

G-protein-coupled receptors signal through cognate G proteins. Despite the widespread importance of these receptors, their regul

 
Dec 06 2022
Dec
06
Parkin coordinates mitochondrial lipid remodeling to execute mitophagy
EMBO Rep. 2022 Dec 6; 23(12):e55191. Epub 2022 Oct 18.
Wei-Yuan Yang

Autophagy has emerged as the prime machinery for implementing organelle quality control. In the context of mitophagy, the ubiqui

 
Dec 06 2022
Nov
05

Three new 2-guanidinoethanesulfonyl sesquiterpene analogues of (−)-agelasidine A, agelasidines G–I (13), were isolated from a m

 
Nov 05 2022
Nov
01

Heat shock protein 90 (Hsp90) is a ubiquitous chaperone to interact with numerous proteins to regulate multiple cellular process

 
Nov 01 2022
Nov
01
Ancestral Archaea Expanded the Genetic Code with Pyrrolysine
Journal of Biological Chemistry. 2022 Nov; 298(11):102521. Epub 2022 Sep 22.
Yane-Shih Wang

The pyrrolysyl-tRNA synthetase (PylRS) facilitates the co-translational installation of the 22nd amino acid pyrrolysine. Owing t

 
Nov 01 2022
Nov
01

Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while th

 
Nov 01 2022
Oct
21

WbbB, a lipopolysaccharide O-antigen synthesis enzyme from Raoultella terrigena, contains an N-terminal glycosyltransferase doma

 
Oct 21 2022
Oct
10

Human cervicovaginal fluid (CVF) is a complex, functionally important and glycan rich biological fluid, fundamental in mediating

 
Oct 10 2022
Oct
07
Helicobacter pylori employs a general protein glycosylation system for the modification of outer membrane adhesins
Gut Microbes. 2022 Jan-Dec; 14(1):2130650. Published online: 07 Oct 2022.
Chun-Hung Lin

Helicobacter pylori infection is associated with the development of several gastric diseases including gastric cancer. To reach

 
Oct 07 2022
Oct
03
Vav independently regulates synaptic growth and plasticity through distinct actin-based processes
J Cell Biol. 2022 Oct 3; 221(10):e202203048. Epub 2022 Aug 17.
Chi-Kuang Yao

Modulation of presynaptic actin dynamics is fundamental to synaptic growth and functional plasticity; yet the underlying molecul

 
Oct 03 2022
Sep
15
AMPK promotes Arf6 activation in a kinase-independent manner upon glucose starvation
J Cell Sci. 2022 Sep 15; 135(18):jcs259609.
Jia-Wei Hsu

AMP-activated protein kinase (AMPK) is a crucial cellular nutrient and energy sensor that maintains energy homeostasis. AMPK als

 
Sep 15 2022
Sep
15
Comparison of ATP-binding pockets and discovery of homologous recombination inhibitors
Bioorg Med Chem. 2022 Sep 15; 70:116923. Epub 2022 Jul 9.
Peter Chi

The ATP binding sites of many enzymes are structurally related, which complicates their development as therapeutic targets. In t

 
Sep 15 2022
Sep
11
Ligand-assisted imprinting-probe-labeling strategy reveals Siglec-7 – glycoprotein interactions
Journal of the Chinese Chemical Society, 2022 Sep 11; 69(8):1326-1337.
Takashi Angata

The development of a ligand-assisted imprinting probe (i.e., LIP 1) for the site-specific labeling of recombinant Siglec-7 prote

 
Sep 11 2022
Sep
09
Synthesis of the Mycobacterium tuberculosis Canetti Lipooligosaccharide II Nonasaccharide
Org Lett. 2022 Sep 9; 24(35):6428-6432. Epub 2022 Aug 25.
Todd L. Lowary

A route for preparing lipooligosaccharide (LOS) glycans from Mycobacterium tuberculosis Canetti was developed and applied to the

 
Sep 09 2022
Sep
08

The edible fungus Tremella fuciformis was shown to have a high molecular weight (1.87 × 103 kDa) bioactive polysaccharide, denot

 
Sep 08 2022
Sep
01

Galectin-3 (GAL3) is a β-galactoside-binding lectin expressed in CD4 T cells infected with human immunodeficiency virus-1 (HIV-1

 
Sep 01 2022
Sep
01
Subtyping of major SARS-CoV-2 variants reveals different transmission dynamics based on 10 million genomes
PNAS Nexus. 2022 Sep 1; 1(4):pgac181. eCollection 2022 Sep.
James C. Liao

SARS-CoV-2 continues to evolve, causing waves of the pandemic. Up to May 2022, 10 million genome sequences have accumulated, whi

 
Sep 01 2022
Aug
29

Eggshell membrane (ESM), a plentiful biological waste, consists of collagen-like proteins and glycosaminoglycans (GAGs) such as

 
Aug 29 2022
Aug
19
In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM
Nat Commun. 2022 Aug 19; 13(1):4877.
Shang-Te Danny Hsu, Kay-Hooi Khoo, Yuan-Chih Chang

Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV). PED cause

 
Aug 19 2022
Aug
17

To transform cellulose from biomass into fermentable sugars for biofuel production requires efficient enzymatic degradation of c

 
Aug 17 2022
Aug
05
A De Novo Route to 3,6-Dideoxy Sugars
Org Lett. 2022 Aug 5; 24(30):5614-5618. Epub 2022 Jul 26.
Todd L. Lowary

We report the de novo asymmetric synthesis of the 3,6-dideoxy sugars abequose, paratose, and tyvelose from 2-acetylfuran. C

 
Aug 05 2022
Aug
05

Application of B-cell receptor (BCR) pathway inhibitor ibrutinib for chronic lymphocytic leukemia (CLL) is a major breakthrough,

 
Aug 05 2022
Aug
03
2-Guanidinoethanesulfonyl sesquiterpenes from the marine sponge Agelas nakamurai
Tetrahedron Letters. 2022 Aug 3; 103, 153964.
Shih Hsiung Wu

A new 2-guanidinoethanesulfonyl sesquiterpene, cyclohexylagelasidine A (1), and a known compound (–)-agelasidine A (2), were iso

 
Aug 03 2022
Aug
03
2.2 Å Cryo-EM Tetra-Protofilament Structure of the Hamster Prion 108-144 Fibril Reveals an Ordered Water Channel in the Center
J Am Chem Soc. 2022 Aug 3; 44(30):13888-13894. Epub 2022 Jul 20.
Rita P.-Y. Chen, Kuen-Phon Wu

Fibrils of the hamster prion peptide (sHaPrP, sequence 108-144) were prepared in an acidic solution, and their structure was sol

 
Aug 03 2022
Aug
01
Nickel superoxide dismutase protects nitrogen fixation in Trichodesmium
Limnology and Oceanography Letters. 2022 Aug 1; 7(4):363-371. Epub 2022 Jun 20.
Shang-Te Danny Hsu

Trichodesmium, a major diazotroph in tropical and subtropical oceans, provides a significant amount of bioavailable nitrogen in

 
Aug 01 2022
Aug
01

Naturally occurring N-glycans display much diversity in modifications, linkages, and peripheral presentation of the oligosacchar

 
Aug 01 2022
Aug
01
Condition-dependent structural collapse in the intrinsically disordered N-terminal domain of prion protein
IUBMB Life. 2022 Aug; 74(8):780-793. Epub 2021 Jul 20.
Rita P.-Y. Chen, Meng-Ru Ho

Prion protein is composed of a structure-unsolved N-terminal domain and a globular C-terminal domain. Under limited trypsin dige

 
Aug 01 2022
Aug
01
Regulation of oxidative stress-induced autophagy by ATG9A ubiquitination
Autophagy. 2022 Aug; 18(8):2008-2010. Epub 2022 Apr 12.
Guang-Chao Chen

High levels of reactive oxygen species (ROS) result in oxidative stress, which damages cells and leads to the development of man

 
Aug 01 2022
Jul
22

We report the isolation and stereochemical determination of the predominant native cholesteryl 6-O-phosphatidyl α-glucoside (CPG

 
Jul 22 2022
Jul
13

N-glycans are diversified by a panel of glycosyltransferases in the Golgi, which are supposed to modify various glycoproteins in

 
Jul 13 2022
Jul
07

Background: With the continuous emergence of new SARS-CoV-2 variants that feature increased transmission and immune escape, ther

 
Jul 07 2022
Jul
06

Background: Metastasis and chemoresistance are major culprits of cancer mortality, but factors contributing to these processes a

 
Jul 06 2022
Jul
01
Biodistribution analysis of an intranasal-delivered peptide by the nanoSPECT/CT imaging
Journal of Drug Delivery Science and Technology. 2022 July; 73, 103454.
Rita P.-Y. Chen

Protein/peptide aggregation-induced cell death is the culprit of many neurodegenerative diseases. Many peptide drugs were design

 
Jul 01 2022
Jun
20

TRIM28 is a scaffold protein that interacts with DNA-binding proteins and recruits corepressor complexes to cause gene silencing

 
Jun 20 2022
Jun
15

The dystrophin-glycoprotein complex connects the cytoskeleton with base membrane components such as laminin through unique O-gly

 
Jun 15 2022
Jun
10

Piperazine-derived diazabicycles are privileged structures found in natural products and synthetic chemical entities, including

 
Jun 10 2022
Jun
06
Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme
Nat Chem. 2022 Jun; 14(6):677-685. Epub 2022 Apr 7.
Ming-Daw Tsai, Manuel Maestre-Reyna, Yoshitaka Bessho, Wen-Jin Wu, Kai-Fa Huang

Flavin coenzymes are universally found in biological redox reactions. DNA photolyases, with their flavin chromophore (FAD), util

 
Jun 06 2022
Jun
06
Tumor suppressor BAP1 nuclear import is governed by transportin-1
J Cell Biol. 2022 Jun 6;221(6):e202201094. Epub 2022 Apr 21.
Shang-Te Danny Hsu, Steven Lin, Kuen-Phon Wu, Shu-Yu Lin

Subcellular localization of the deubiquitinating enzyme BAP1 is deterministic for its tumor suppressor activity. While the monou

 
Jun 06 2022
Jun
01

Prion diseases are transmissible fatal neurodegenerative disorders spreading between humans and other mammals. The pathogenic ag

 
Jun 01 2022
May
31
Molecular Basis and Role of Siglec-7 Ligand Expression on Chronic Lymphocytic Leukemia B Cells
Front Immunol. 2022 May 31; 13:840388. eCollection 2022.
Takashi Angata, Kay-Hooi Khoo

Siglec-7 (sialic acid–binding immunoglobulin-like lectin 7) is an immune checkpoint-like glycan recognition protein on natural k

 
May 31 2022
May
31
Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose
Front Chem. 2022 May 31; 10:905105. eCollection 2022.
Ching-Ching Yu

We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with

 
May 31 2022
May
20
Preparation of Cas9 Ribonucleoproteins for Genome Editing
Bio-protocol. 2022 May 20;12(10): e4420.
Steven Lin, Sheng-Wei Lin

Genome editing by the delivery of pre-assembled Cas9 ribonucleoproteins (Cas9 RNP) is an increasingly popular approach for cell

 
May 20 2022
May
18

Data from the World Health Organisation show that the global incidence of dengue infection has risen drastically, with an estima

 
May 18 2022
May
16
SARS-CoV-2 3CLpro displays faster self-maturation in vitro than SARS-CoV 3CLpro due to faster C-terminal cleavage
FEBS Lett. 2022 May 16; 596(9):1214-1224. Epub 2022 Mar 28.
Po-Huang Liang

The coronavirus (CoV) disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) has bec

 
May 16 2022
May
15
Impacts of cancer-associated mutations on the structure-activity relationship of BAP1
Journal of Molecular Biology. 2022 May 15; 434(9):167553. Epub 2022 Mar 19.
Shang-Te Danny Hsu

BRAC1 associated protein-1 (BAP1) is a major tumor suppressor involved in many cancers. The deubiquitinase (DUB) activity of BAP

 
May 15 2022
May
12

Bacterial polyynes are highly active natural products with a broad spectrum of antimicrobial activities. However, their detailed

 
May 12 2022
May
10

Background: Autophagy plays important roles in cell homeostasis and protein quality control. Long non-coding RNAs (lncRNAs) have

 
May 10 2022
May
01
Synergic action of an inserted carbohydrate-binding module in a glycoside hydrolase family 5 endoglucanase
Acta Crystallogr D Struct Biol. 2022 May 1; 78(Pt 5):633-646. Epub 2022 Apr 20.
Shih-Hsiung Wu, Kai-Fa Huang

Most known cellulase-associated carbohydrate-binding modules (CBMs) are attached to the N- or C-terminus of the enzyme or are ex

 
May 01 2022
May
01
The biosynthetic origin of ribofuranose in bacterial polysaccharides
Nat Chem Biol. 2022 May; 18(5):530-537. Epub 2022 Apr 7.
Todd L. Lowary

Bacterial surface polysaccharides are assembled by glycosyltransferase enzymes that typically use sugar nucleotide or polyprenyl

 
May 01 2022
May
01
Structural basis for the helical filament formation of Escherichia coli glutamine synthetase
Protein Science. 2022 May; 31(5):e4304. Epub 2022 April 11.
Kuen-Phon Wu, Meng-Ru Ho

Escherichia coli glutamine synthetase (EcGS) spontaneously forms a dodecamer that catalytically converts glutamate to glutamine.

 
May 01 2022
May
01
BARD1 is an ATPase activating protein for OLA1
Biochim Biophys Acta Gen Subj. 2022 May; 1866(5):130099. Epub 2022 Feb 5.
Shang-Te Danny Hsu

OLA1 is a P-loop ATPase, implicated in centrosome duplication through the interactions with tumor suppressors BRCA1 and BARD1. D

 
May 01 2022
Apr
09
Oxidation of catalytic cysteine of human deubiquitinase BAP1 triggers misfolding and aggregation in addition to functional loss
Biochemical and Biophysical Research Communications. 2022 April 9; 599, 57-62. Epub 2022 February 10.
Shang-Te Danny Hsu

Deubiquitinating enzymes (DUBs) form a large protease family involved in a myriad of biological and pathological processes, incl

 
Apr 09 2022
Apr
04
Zebrafish Establish Female Germ Cell Identity by Advancing Cell Proliferation and Meiosis
Front Cell Dev Biol. 2022 Apr 4; 10:866267. eCollection 2022.
Jui-Hsia Weng

Zebrafish is a popular research model; but its mechanism of sex determination is unclear and the sex of juvenile fish cannot be

 
Apr 04 2022
Apr
01
Electrospray ionization of native membrane proteins proceeds via a charge equilibration step
RSC Adv. 2022 Apr 1; 12(16):9671-9680. eCollection 2022 Mar 25.
Hsin-Yung Yen

Electrospray ionization mass spectrometry is increasingly applied to study the structures and interactions of membrane protein c

 
Apr 01 2022
Apr
01
One-Pot Regioselective Diacylation of Pyranoside 1,2-cis Diols
J Org Chem. 2022 Apr 1 ;87(7):4894-4907.
Todd L. Lowary

A one-pot strategy for functionalizing pyranoside 1,2-cis-diols with two different ester protecting groups is reported. The appr

 
Apr 01 2022
Apr
01

Wild tomatoes (Solanum peruvianum) are important genomic resources for tomato research and breeding. Development of a foreign DN

 
Apr 01 2022
Apr
01
Performance of the new biological small- and wide-angle X-ray scattering beamline 13A at the Taiwan Photon Source
Journal of Applied Crystallography. 2022 Apr; 55(2):340-352.
Meng-Chiao Ho

Recent developments in the instrumentation and data analysis of synchrotron small-angle X-ray scattering (SAXS) on biomolecules

 
Apr 01 2022
Mar
31
Assembly mechanism of the pleomorphic immature poxvirus scaffold
Nat Commun. 2022 Mar 31; 13(1):1704.
Matthias Wolf

In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that

 
Mar 31 2022
Mar
25

Nature Communications Editors’ Highlights - “Microbiology and infectious diseases”

The combination of EGF, CHIR99021, A83-01, SB4

 
Mar 25 2022
Mar
22

Background: Colorectal cancer (CRC) is a worldwide cancer with rising annual incidence. New medications for patients with CRC ar

 
Mar 22 2022
Mar
21

Klebsiella pneumoniae is an important pathogen associated with nosocomial infection and has developed increasing resistance to a

 
Mar 21 2022
Mar
15

Enzymatic synthesis that is commenced by the sugar nucleotide regeneration system (SNRS) protocol can minimize 1) the consumptio

 
Mar 15 2022
Feb
24
A cell-free self-replenishing CO2-fixing system
Nature Catalysis. 2022 February 24; 5(2);154-162.
James C. Liao

Biological CO2 fixation is so far the most effective means for CO2 reduction at scale and accounts for most of the CO2 fixed on

 
Feb 24 2022
Feb
22
K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy
Cell Reports. 2022 February 22; 38(8):110354.
Guang-Chao Chen, Shu-Yu Lin, Chin-Chun Hung

Excessive generation and accumulation of highly reactive oxidizing molecules causes oxidative stress and oxidative damage to cel

 
Feb 22 2022
Feb
15
Structural Basis for the Peptidoglycan-Editing Activity of YfiH
mBio. 2022 Feb 15; 13(1),e03646-21.
Chung-I Chang

Bacterial cells are encased in peptidoglycan (PG), a polymer of disaccharide N-acetylglucosamine (GlcNAc) and N-acetyl-muramic a

 
Feb 15 2022
Feb
15

Klebsiella pneumoniae serotype KN2 is a carbapenem-resistant strain and leads to the health care-associated infections, such as

 
Feb 15 2022
Feb
07
Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use
J Biomed Sci. 2022 Feb 7; 29(1):9.
Shih-Hsiung Wu, Kai-Fa Huang, Wen-Jin Wu, Meng-Ru Ho, Andrew H-J Wang

Background: K1 capsular polysaccharide (CPS)-associated Klebsiella pneumoniae is the primary cause of pyogenic liver abscesses (

 
Feb 07 2022
Feb
06
Carbohydrate Ligands for COVID-19 Spike Proteins
Viruses. 2022 Feb 6; 14(2):330.
Chun-Hung Lin, Zhi-Jay Tu

An outbreak of SARS-CoV-2 coronavirus (COVID-19) first detected in Wuhan, China, has created a public health emergency all over

 
Feb 06 2022
Feb
01

Specific antibody interactions with short peptides have made epitope tagging systems a vital tool employed in virtually all fiel

 
Feb 01 2022
Jan
31

Extensive glycosylation of the spike protein of severe acute respiratory syndrome coronavirus 2 virus not only shields the major

 
Jan 31 2022
Jan
30

Trimeresurus gracilis (Tgc) is endemic to Taiwan and shown to be closely related with Ovophis okinavensis by previous phylogenet

 
Jan 30 2022
Jan
12

Since 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been rapidly spreading worldwide, causing hundreds

 
Jan 12 2022
Jan
10

Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological

 
Jan 10 2022
Jan
10

T-Cell Protein Tyrosine Phosphatase (TCPTP, PTPN2) is a non-receptor type protein tyrosine phosphatase that is ubiquitously expr

 
Jan 10 2022
Jan
03
Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2
Nat Chem Biol. 2022 Jan; 18(1):81-90. Epub 2021 Nov 9.
Todd L. Lowary, Shang-Te Danny Hsu

Emerging evidence suggests that host glycans influence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. H

 
Jan 03 2022

2021

Dec
28

The T-cell protein tyrosine phosphatase (TCPTP/PTPN2) targets a broad variety of substrates across different subcellular compart

 
Dec 28 2021
Dec
20

Mass spectrometry-based high-sensitivity mapping of terminal glycotopes relies on diagnostic MS2 and/or MS3 ions that can differ

 
Dec 20 2021
Dec
10
Harnessing fluorescent moenomycin A antibiotics for bacterial cell wall imaging studies
Chembiochem. 2021 Dec 10; 22(24):3462-3468. Epub 2021 Oct 4. doi: 10.1002/cbic.202100433
Yu-Ling Shih

The imaging of peptidoglycan (PGN) dynamics in living bacteria facilitates the understanding of PGN biosynthesis and wall-target

 
Dec 10 2021
Dec
08
Evidence for an Enzyme-Catalyzed Rauhut–Currier Reaction during the Biosynthesis of Spinosyn A
J Am Chem Soc. 2021 Dec 8; 143(48):20291-20295. Epub 2021 Nov 23. doi: 10.1021/jacs.1c09482
Ming-Daw Tsai

The catalog of enzymes known to catalyze the nucleophile-assisted formation of C-C bonds is extremely small, and there is presen

 
Dec 08 2021
Dec
07
ZIC-cHILIC-Based StageTip for Simultaneous Glycopeptide Enrichment and Fractionation toward Large-Scale N-Sialoglycoproteomics
Anal Chem. 2021 Dec 7; 93(48):15931-15940. Epub 2021 Nov 15. doi: 10.1021/acs.analchem.1c03224
Kay-Hooi Khoo

Alterations of protein glycosylation are closely related with pathophysiological regulation. Due to the structural macro- and mi

 
Dec 07 2021
Nov
25

This study reports the application of expanding genetic codes in developing protein cage-based delivery systems. The evolved Met

 
Nov 25 2021
Nov
24

Background: RAD51-dependent homologous recombination (HR) is one of the most important pathways for repairing DNA double-strand

 
Nov 24 2021
Nov
23

The mechanisms that underlie the extensive phenotypic diversity in genetic disorders are poorly understood. Here, we develop a l

 
Nov 23 2021
Nov
19
Carbohydrate Sulfation As a Mechanism for Fine-Tuning Siglec Ligands
ACS Chem Biol. 2021 Nov 19; 16(11):2673-2689. Epub 2021 Oct 18.
Kay-Hooi Khoo

The immunomodulatory family of Siglecs recognizes sialic acid-containing glycans as “self”, which is exploited in cancer for imm

 
Nov 19 2021
Nov
19
Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans
Biochem Biophys Res Commun. 2021 Nov 19; 579:8-14. Epub 2021 Sep 21.
Kay-Hooi Khoo

α-Dystroglycan (α-DG) is a glycoprotein specifically modified with O-mannosyl glycans bearing long polysaccharides, termed matri

 
Nov 19 2021
Nov
15
Synthesis of a Tridecasaccharide Lipooligosaccharide Antigen from the Opportunistic Pathogen Mycobacterium kansasii
Angew Chem Int Ed Engl. 2021 Nov 15; 60(47):24859-24863. Epub 2021 Oct 21.
Todd L. Lowary

The outer surfaces of mycobacteria, including the organism that causes tuberculosis, are decorated with an array of immunomodula

 
Nov 15 2021
Nov
10
Processive cleavage of substrate at individual proteolytic active sites of the Lon protease complex
Science Advances. 2021 Nov 10; 7(46), eabj9537. Epub 2021 Nov 10.
Chung-I Chang, Kai-Fa Huang

The Lon protease is the prototype of a family of proteolytic machines with adenosine triphosphatase modules built into a substra

 
Nov 10 2021
Nov
05

Replication stress causes replication fork stalling, resulting in an accumulation of single-stranded DNA (ssDNA). Replication pr

 
Nov 05 2021
Nov
01

Glycoproteomics is a powerful yet analytically challenging research tool. Software packages aiding the interpretation of complex

 
Nov 01 2021
Oct
27

Background: During autophagy defense against invading microbes, certain lipid types are indispensable for generating specialized

 
Oct 27 2021
Oct
21
Structure-guided antibody cocktail for prevention and treatment of COVID-19
PLOS PATHOGENS. 2021 Oct 21; 17(10), e1009704.
Shang-Te Danny Hsu, Yuan-Chih Chang

Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies ar

 
Oct 21 2021
Oct
20

Histone arginine methylation is a key post-translational modification that mediates epigenetic events that activate or repress g

 
Oct 20 2021
Oct
16
D614G mutation in the SARS-CoV-2 spike protein enhances viral fitness by desensitizing it to temperature-dependent denaturation
Journal of Biological Chemistry. 2021 Oct 16; 297(4):101238.
Shang-Te Danny Hsu, Yuan-Chih Chang

The D614G mutation in the spike protein of SARS-CoV-2 alters the fitness of the virus, leading to the dominant form observed in

 
Oct 16 2021
Oct
15
Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration
CRISPR J. 2021 Oct 15; 4(5):752-760. Epub 2021 Sep 27.
Steven Lin

Versatile genome editing can be facilitated by the insertion of DNA sequences into specific locations. Current protocols involvi

 
Oct 15 2021
Oct
15
Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA
Sci Adv . 2021 Oct 15;7(42):eabj4565. Epub 2021 Oct 15.
Todd L. Lowary

Glycolipids are prominent components of bacterial membranes that play critical roles not only in maintaining the structural inte

 
Oct 15 2021
Oct
15
Complete three-dimensional structures of the Lon protease translocating a protein substrate
Science Advances. 2021 Oct 15; 7(42):eabj7835. Epub 2021 Oct 15.
Chung-I Chang

Lon is an evolutionarily conserved proteolytic machine carrying out a wide spectrum of biological activities by degrading misfol

 
Oct 15 2021
Oct
13
PTPN9-mediated dephosphorylation of VTI1B promotes ATG16L1 precursor fusion and autophagosome formation
Autophagy. 2021 Oct; 17(10):2750-2765. Epub 2020 Oct 28.
Guang-Chao Chen, Chin-Chun Hung, Shu-Yu Lin

Macroautophagy/autophagy is an evolutionarily conserved intracellular pathway for the degradation of cytoplasmic materials. Unde

 
Oct 13 2021
Oct
12

The surface polysacharide arabinomannan (AM) and related glycolipid lipoarabinomannan (LAM) play critical roles in tuberculosis

 
Oct 12 2021
Oct
11
Molecular basis for ATPase-powered substrate translocation by the Lon AAA+ protease
Journal of Biological Chemistry. 2021 Oct; 297(4), 101239.
Chung-I Chang

The Lon AAA+ (adenosine triphosphatases associated with diverse cellular activities) protease (LonA) converts ATP-fuelled confor

 
Oct 11 2021
Sep
30

Plant diseases are important issues in agriculture, and the development of effective and environment-friendly means of disease c

 
Sep 30 2021
Sep
28

The biosynthesis of lipopolysaccharide (LPS), a key immunomodulatory molecule produced by gram-negative bacteria, has been a top

 
Sep 28 2021
Sep
16

Natural killer (NK) cells are potent innate immune cells that provide the surveillance and elimination of infected, stressed, an

 
Sep 16 2021
Sep
15

Background: Plectranthus amboinicus (Spreng.) is an herb commonly used in folk medicine and food by several Asian countries. The

 
Sep 15 2021
Sep
01
Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function
Nat Struct Mol Biol. 2021 Sep; 28(9):731-739. Epub 2021 Aug 12.
Shang-Te Danny Hsu, Yuan-Chih Chang, Meng-Ru Ho

The B.1.1.7 variant of SARS-CoV-2 first detected in the UK harbors amino-acid substitutions and deletions in the spike protein t

 
Sep 01 2021
Aug
18

Human PUF-A/PUM3 is a RNA and DNA binding protein participating in the nucleolar processing of 7S to 5.8S rRNA. The nucleolar lo

 
Aug 18 2021
Aug
17

Meiotic recombination plays dual roles in the evolution and stable inheritance of genomes: Recombination promotes genetic divers

 
Aug 17 2021
Aug
03

Background: Alzheimer's disease (AD) involves impairment of Aβ clearance. Neprilysin (NEP) is the most efficient Aβ peptidase. E

 
Aug 03 2021
Jul
30

The semi-fossil and pit-less Azemiops feae is possibly the most primitive crotalid species. Here, we have cloned and sequenced c

 
Jul 30 2021
Jul
29

The emergence of the genotype (G) 2 and re-emergence of the G1 porcine epidemic diarrhea virus (PEDV) has caused severe economic

 
Jul 29 2021
Jul
27

Cytosolic lipopolysaccharides (LPSs) bind directly to caspase-4/5/11 through their lipid A moiety, inducing inflammatory caspase

 
Jul 27 2021
Jul
26
Preparation of High-Temperature Sample Grids for Cryo-EM
Jove - Journal of Visualized Experiments. 2021 Jul 26; 173, e62772.
Ming-Daw Tsai, Yuan-Chih Chang

The sample grids for cryo-electron microscopy (cryo-EM) experiments are usually prepared at a temperature optimal for the storag

 
Jul 26 2021
Jul
21
Structure-activity relationship of avocadyne
Food Funct. 2021 Jul 21; 12(14):6323-6333. Epub 2021 Jun 6.
Todd L. Lowary

Avocado consumption is associated with numerous health benefits. Avocadyne is a terminally unsaturated, 17-carbon long acetogeni

 
Jul 21 2021
Jul
08
TIFA protein expression is associated with pulmonary arterial hypertension
Sci Rep. 2021 Jul 8; 11(1):14140.
Ming-Daw Tsai

Tumor necrosis factor receptor-associated factor-interacting protein with a forkhead-associated domain (TIFA), a key regulator o

 
Jul 08 2021
Jul
01
Genetically encoded multivalent liquid glycan array displayed on M13 bacteriophage
Nat Chem Biol. 2021 Jul; 17(7):806-816. Epub 2021 May 6.
Todd L. Lowary

The central dogma of biology does not allow for the study of glycans using DNA sequencing. We report a liquid glycan array (LiGA

 
Jul 01 2021
Jun
25

Toll-like receptor 4 (TLR4) recognizes various protein ligands; however, the protein-TLR4 binding model is unclear. Here we demo

 
Jun 25 2021
Jun
25
A unique carboxylic-acid hydrogen-bond network (CAHBN) confers glutaminyl cyclase activity on M28 family enzymes
J Mol Biol. 2021 Jun 25; 433(13):166960. Epub 2021 Mar 25.
Kai-Fa Huang, Andrew H-J Wang

Proteins with sequence or structure similar to those of di-Zn exopeptidases are usually classified as the M28-family enzymes, in

 
Jun 25 2021
Jun
18

Searching for new antimicrobials is a pressing issue to conquer the emergence of multidrug-resistant (MDR) bacteria and fungi. A

 
Jun 18 2021
Jun
15

The outbreak of coronavirus (CoV) disease 2019 (COVID-19) caused by the severe acute respiratory syndrome CoV-2 (SARS-CoV-2) has

 
Jun 15 2021
Jun
14

d-glycero-d-manno-Heptose-1β,7-bisphosphate (HBP) is a bacterial metabolite that can induce a TIFA-dependent innate immune respo

 
Jun 14 2021
Jun
11

Enterococcus faecalis, a member of the commensal flora in the human gastrointestinal tract, has become a threatening nosocomial

 
Jun 11 2021
Jun
07

Background: Coronavirus disease 19 (COVID-19) first appeared in the city of Wuhan, in the Hubei province of China. Since its eme

 
Jun 07 2021
Jun
05
The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain
J Inflamm (Lond). 2021 Jun 5; 18(1):22.
Ching-Jin Chang, Steven Lin, Sheng-Wei Lin

Background: Tristetraprolin (TTP) family proteins contain conserved tandem CCCH zinc-finger binding to AU-rich elements and C-te

 
Jun 05 2021
Jun
04
Structural polymorphism and substrate promiscuity of a ribosome-associated molecular chaperone
Magnetic Resonance. 2021 Jun 4; 2(1), 375-386.
Shang-Te Danny Hsu, Meng-Ru Ho

Trigger factor (TF) is a highly conserved multi-domain molecular chaperone that exerts its chaperone activity at the ribosomal t

 
Jun 04 2021
Jun
01

Lotus (Nelumbo nucifera Gaertn.) seeds are rich in proteins and are considered to be a beneficial source of plant proteins. In t

 
Jun 01 2021
Jun
01
Converging experimental and computational views of the knotting mechanism of a small knotted protein
Biophys J. 2021 Jun 1; 120(11), 2276-2286. Epub 2021 Apr 1.
Shang-Te Danny Hsu

MJ0366 from Methanocaldococcus jannaschii is the smallest topologically knotted protein known to date. 92 residues in length, MJ

 
Jun 01 2021
Jun
01

The conserved bilateral habenular nuclei (HA) in vertebrate diencephalon develop into compartmentalized structures containing ne

 
Jun 01 2021
May
26
Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir
ACS Cent Sci. 2021 May 26; 7(5):792-802. Epub 2021 Apr 15.
Po-Huang Liang, Kuen-Phon Wu

The outbreak of coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2),

 
May 26 2021
May
24
Tying up the Loose Ends: A Mathematically Knotted Protein
Frontiers in Chemistry. 2021 May 24. 9, 663241.
Shang-Te Danny Hsu

Knots have attracted scientists in mathematics, physics, biology, and engineering. Long flexible thin strings easily knot and ta

 
May 24 2021
May
04
COVID-19 serological survey using micro blood sampling
Sci Rep. 2021 May 4; 11(1):9475.
Matthias Wolf

During August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Tec

 
May 04 2021
May
01

Sialic acid (SA) on the surface of cells is indispensable in numerous physiological and pathological processes, and sensitive an

 
May 01 2021
May
01

Primary metabolism in cyanobacteria is built on the Calvin-Benson-Bassham (CBB) cycle, oxidative pentose phosphate (OPP) pathway

 
May 01 2021
Apr
30

The Lon AAA+ protease (LonA) is a ubiquitous ATP-dependent proteolytic machine, which selectively degrades damaged proteins or n

 
Apr 30 2021
Apr
28

Seneca Valley virus (SVV) is a picornavirus with potency in selectively infecting and lysing cancerous cells. The cellular recep

 
Apr 28 2021
Apr
27

Mitochondria are dynamic organelles as they continuously undergo fission and fusion. These dynamic processes conduct not only mi

 
Apr 27 2021
Apr
16

Redox-dependent inactivation of deubiquitinases (DUBs) is a critical factor for attenuating their DUB activity in response to ce

 
Apr 16 2021
Apr
13
Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy Aided by a Small Scaffold Protein
Biochemistry. 2021 Apr 13; 60(14):1075-1079. Epub 2021 Mar 15.
Shang-Te Danny Hsu, Kuen-Phon Wu, Meng-Ru Ho

Cryo-electron microscopy (cryo-EM)-based structure determination of small proteins is hindered by the technical challenges assoc

 
Apr 13 2021
Apr
12
Probing bacterial cell wall growth by tracing wall-anchored protein complexes
Nat Commun. 2021 Apr 12; 12(1):2160.
Yu-Ling Shih

The dynamic assembly of the cell wall is key to the maintenance of cell shape during bacterial growth. Here, we present a method

 
Apr 12 2021
Mar
26
A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane
Sci Adv. 2021 Mar 26; 7(13):eabc6345. Print 2021 Mar.
Wei Yuan Yang

Cryo-electron tomography (cryo-ET) provides structural context to molecular mechanisms underlying biological processes. Although

 
Mar 26 2021
Mar
24

Partially acetylated chito-oligosaccharides (paCOSs) are bioactive compounds with potential medical applications. Their biologic

 
Mar 24 2021
Mar
18
Kinetic characterization and inhibitor screening for the proteases leading to identification of drugs against SARS-CoV-2
Antimicrob Agents Chemother. 2021 Mar 18; 65(4):e02577-20. Print 2021 Mar 18.
Po-Huang Liang

Coronavirus (CoV) disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has claim

 
Mar 18 2021
Mar
08
ALS2 regulates endosomal trafficking, postsynaptic development, and neuronal survival
J Cell Biol . 2021 May 8; 220(5):e202007112.
Chi-Kuang Yao

Mutations in the human ALS2 gene cause recessive juvenile-onset amyotrophic lateral sclerosis and related motor neuron diseases.

 
Mar 08 2021
Mar
05
An Enzyme-Mediated Aza-Michael Addition Is Involved in the Biosynthesis of an Imidazoyl Hybrid Product of Conidiogenone B
Org Lett. 2021 Mar 5; 23(5):1904-1909. Epub 2021 Feb 11.
Shih-Hsiung Wu, Hsiao-Ching Lin

Meleagrin B is a terpene-alkaloid hybrid natural product that contains both the conidiogenone and meleagrin scaffold. Their deri

 
Mar 05 2021
Mar
04

Irinotecan inhibits cell proliferation and thus is used for the primary treatment of colorectal cancer. Metabolism of irinotecan

 
Mar 04 2021
Mar
01

Genome editing is a powerful technique for delineating complex signaling circuitry and enhancing the functionality of immune cel

 
Mar 01 2021
Feb
28

Collagen is a major structural component of the extracellular matrix and connective tissue. The key structural feature of collag

 
Feb 28 2021
Feb
26
VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism
Nat Commun. 2021 Feb 26; 12(1):1322.
Ruey-Hwa Chen, Guang-Chao Chen, Kuen-Phon Wu, Shu-Yu Lin

The ubiquitin-proteasome system (UPS) and autophagy are two major quality control processes whose impairment is linked to a wide

 
Feb 26 2021
Feb
26
Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells
J Biol Chem. Jan-Jun 2021; 296:100477. Epub 2021 Feb 26.
Takashi Angata

Sialic acid (Sia)-binding immunoglobulin-like lectin 7 (Siglec-7) is an inhibitory receptor primarily expressed on natural kille

 
Feb 26 2021
Feb
23
DMTMM-Mediated Intramolecular Cyclization of Acidic Residues in Peptides/Proteins
ACS Omega . 2021 Feb 23; 6(7):4708-4718.
Andrew H-J Wang

The formation of succinimide in proteins has attracted considerable attention in protein aging and biopharmaceutical research. T

 
Feb 23 2021
Feb
23
Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences
Proc Natl Acad Sci U S A . 2021 Feb 23;118(8):e2007192118.
Peter Chi

Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombin

 
Feb 23 2021
Feb
19
Single nucleotide variants lead to dysregulation of the human mitochondrial NAD(P)+-dependent malic enzyme
iScience. 2021 Jan 13; 24(2):102034. eCollection 2021 Feb 19.
Meng-Chiao Ho

Human mitochondrial NAD(P)+-dependent malic enzyme (ME2) is well recognized to associate with cancer cell metabolism, and the si

 
Feb 19 2021
Feb
19

Interleukin-1β (IL-1β) is a potent pleiotropic cytokine playing a central role in protecting cells from microbial pathogen infec

 
Feb 19 2021
Feb
12
Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization
Sci Adv. 2021 Feb 12; 7(7):eabc6093. Print 2021 Feb.
Ruey-Hwa Chen, Shu-Yu Lin

The role of protein stabilization in cortical development remains poorly understood. A recessive mutation in the USP11 gene is f

 
Feb 12 2021
Feb
01
Acetyltransferase Enok regulates transposon silencing and piRNA cluster transcription
PLoS Genet . 2021 Feb 1; 17(2):e1009349. eCollection 2021 Feb.
Fu Huang

The piRNA pathway is a highly conserved mechanism to repress transposon activation in the germline in Drosophila and mammals. Th

 
Feb 01 2021
Feb
01
Vibrio cholerae biofilm scaffolding protein RbmA shows an intrinsic, phosphate‐dependent autoproteolysis activity
IUBMB Life. 2021 Feb; 73(2):418-431. Epub 2020 Dec 28.
Andrew H-J Wang, Manuel Maestre-Reyna, Ming-Daw Tsai, Wen-Jin Wu, Yoshitaka Bessho

Vibrio cholerae is the causative agent of the diarrheal disease cholera, for which biofilm communities are considered to be envi

 
Feb 01 2021
Feb
01
Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression
Dev Biol. 2021 Feb; 470:21-36. Epub 2020 Nov 13.
Chang-Jen Huang

Nodal signaling is essential for mesoderm and endoderm formation, as well as neural plate induction and establishment of left-ri

 
Feb 01 2021
Jan
26

The infectious SARS-CoV-2 causes COVID-19, which is now a global pandemic. Aiming for effective treatments, we focused on the ke

 
Jan 26 2021
Jan
15

Degradation and collapse of stalled replication forks are main sources of genomic instability, yet the molecular mechanisms for

 
Jan 15 2021
Jan
15
Use of Synthetic Glycolipids to Probe the Number and Position of Arabinan Chains on Mycobacterial Arabinogalactan
ACS Chem Biol. 2021 Jan 15; 16(1):20-26. Epub 2020 Dec 31.
Todd L. Lowary

The arabinogalactan of Corynebacterianeae is a critical heteropolysaccharide that tethers outer membrane mycolic acids to peptid

 
Jan 15 2021
Jan
15
Crystal structure of the N-terminal domain of TagH reveals a potential drug targeting site
Biochem Biophys Res Commun. 2021 Jan 15; 536:1-6. Epub 2020 Dec 22.
Andrew H-J Wang

Bacterial wall teichoic acids (WTAs) are synthesized intracellularly and exported by a two-component transporter, TagGH, compris

 
Jan 15 2021
Jan
14
Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
Nat Commun. 2021 Jan 14; 12(1):115.
Ming-Daw Tsai, Peter Chi, Meng-Chiao Ho, Wen-Jin Wu

Both high-fidelity and mismatch-tolerant recombination, catalyzed by RAD51 and DMC1 recombinases, respectively, are indispensabl

 
Jan 14 2021
Jan
11

To circumvent the devastating pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, a human

 
Jan 11 2021
Jan
05

Background: The accumulation of lipid-laden macrophages, foam cells, within sub-endothelial intima is a key feature of early ath

 
Jan 05 2021
Jan
02
New Hydroquinone Monoterpenoid and Cembranoid-Related Metabolites from the Soft Coral Sarcophyton tenuispiculatum
Mar Drugs . 2021 Jan; 19(1):E8. Published: 27 December 2020.
Shih-Hsiung Wu

Chemical investigation of the marine soft coral Sarcophyton tenuispiculatum resulted in the isolation of a 1,4-dihydrobenzoquino

 
Jan 02 2021
Jan
02
Structure, catalysis, and inhibition mechanism of prenyltransferase
IUBMB Life. 2021 Jan. 73(1):40-63, First published: 27 November 2020.
Andrew H-J Wang

Isoprenoids, also known as terpenes or terpenoids, represent a large family of natural products composed of five-carbon isopente

 
Jan 02 2021
Jan
02
Sialylation of CD55 by ST3GAL1 facilitates immune evasion in cancer
Cancer Immunol Res. 2021 Jan; 9(1):113-122, Published first November 11, 2020.
Kay-Hooi Khoo

Altered glycosylations, which are associated with expression and activities of glycosyltransferases, can dramatically affect the

 
Jan 02 2021
Jan
01

Protein arginine methyltransferases (PRMTs) are essential epigenetic and post-translational regulators in eukaryotic organisms.

 
Jan 01 2021
Jan
01
A Guillain-Barré syndrome-associated SIGLEC10 rare variant impairs its recognition of gangliosides
J Autoimmun. 2021 Jan; 116:102571. Epub 2020 Nov 19.
Takashi Angata

Guillain-Barré syndrome (GBS), including its variant Miller Fisher syndrome (MFS), is an acute peripheral neuropathy that involv

 
Jan 01 2021
Jan
01

Synthesis of type I LacNAc (Galβ1 → 3GlcNAc) oligosaccharides usually suffers from low yields. We herein report the efficient sy

 
Jan 01 2021
Jan
01

Reactivation of fetal hemoglobin (HbF) expression by therapeutic agents has been suggested as an alternative treatment to modula

 
Jan 01 2021

2020

Dec
27
Multimeric TAT peptides are effective in vitro inhibitors of Staphylococcus saprophyticus
Chem Biol Drug Des. 2020 Dec; 96(6):1348-1354. Epub 2020 Jun 20.
Shih-Hsiung Wu

TAT (48–60) is a tridecapeptide from the envelope protein of HIV that was previously shown to possess cell-penetrating propertie

 
Dec 27 2020
Dec
21

Chaperonin-containing TCP-1 (CCT) is a chaperonin composed of eight subunits that participates in intracellular protein folding.

 
Dec 21 2020
Dec
16

Uropathogenic E scherichia coli (UPEC) is the most common pathogen of urinary tract infections (UTIs). Antibiotic therapy is the

 
Dec 16 2020
Dec
15

Glucuronoxylomannan (AAPS) from the edible wood ear mushroom Auricularia auricula-judae has been demonstrated to exhibit immunos

 
Dec 15 2020
Dec
15

Ligand 1 was the first reported example of monomeric high-affinity synthetic CD22 ligand that regulated B cell activation in vit

 
Dec 15 2020
Dec
10

Synaptic vesicle (SV) endocytosis is coupled to exocytosis to maintain SV pool size and thus neurotransmitter release. Intense s

 
Dec 10 2020
Dec
01
Analysis of genomic distributions of SARS-CoV-2 reveals a dominant strain type with strong allelic associations
Proc Natl Acad Sci U S A. 2020 Dec 1; 117(48):30679-30686. Epub 2020 Nov 12.
James C. Liao

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causal agent of COVID 19, continues to evolve since its first

 
Dec 01 2020
Nov
24

Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant

 
Nov 24 2020
Nov
18

Pseudaminic acid (Pse), a unique carbohydrate in surface-associated glycans of pathogenic bacteria, has pivotal roles in virulen

 
Nov 18 2020
Nov
17
Chlorovirus PBCV-1 protein A064R has three of the transferase activities necessary to synthesize its capsid protein N-linked glycans
Proc Natl Acad Sci U S A. 2020 Nov 17; 117(46):28735-28742. Epub 2020 Nov 2.
Todd L. Lowary

Paramecium bursaria chlorella virus-1 (PBCV-1) is a large double-stranded DNA (dsDNA) virus that infects the unicellular green a

 
Nov 17 2020
Nov
09
Branched Ubiquitination: Detection Methods, Biological Functions and Chemical Synthesis
Molecules. 2020 Nov 9; 25(21):5200.
Ruey-Hwa Chen, Yane-Shih Wang, Kuen-Phon Wu

Ubiquitination is a versatile posttranslational modification that elicits signaling roles to impact on various cellular processe

 
Nov 09 2020
Nov
02

BACKGROUND. Inadequate tuberculosis (TB) diagnostics are a major hurdle in the reduction of disease burden, and accurate point-o

 
Nov 02 2020
Nov
01

Borneol is a bicyclic plant monoterpene. It can be degraded by soil microorganisms through the conversion of borneol dehydrogena

 
Nov 01 2020
Oct
27

Neuromuscular junctions (NMJs) govern efficient neuronal communication with muscle cells, relying on proper architecture of spec

 
Oct 27 2020
Oct
19
Hsc70/Stub1 promotes the removal of individual oxidatively stressed peroxisomes
Nat Commun. 2020 Oct 19; 11(1):5267.
Wei Yuan Yang, Steven Lin

Peroxisomes perform beta-oxidation of branched and very-long chain fatty acids, which leads to the formation of reactive oxygen

 
Oct 19 2020
Oct
15
Nonhydrolyzable Heptose Bis- and Monophosphate Analogues Modulate Pro-inflammatory TIFA-NF-κB Signaling
Chembiochem. 2020 Oct 15; 21(20):2982-2990. Epub 2020 Jul 2.
Ming-Daw Tsai

D-Glycero-D-manno-heptose-1β,7-bisphosphate (HBP) and D-glycero-D-manno-heptose-1β-phosphate (H1P) are bacterial metabolites tha

 
Oct 15 2020
Oct
13
Revealing USP7 Deubiquitinase Substrate Specificity by Unbiased Synthesis of Ubiquitin Tagged SUMO2
Biochemistry. 2020 Oct 13; 59(40):3796-3801. Epub 2020 Oct 2.
Yane-Shih Wang, Shu-Yu Lin

Ubiquitination and SUMOylation of protein are crucial for various biological responses. The recent unraveling of cross-talk betw

 
Oct 13 2020
Oct
08

Prion protein amyloid aggregates are associated with infectious neurodegenerative diseases, known as transmissible spongiform en

 
Oct 08 2020
Oct
07

Extracellular particles (EPs) including extracellular vesicles (EVs) and exomeres play significant roles in diseases and therape

 
Oct 07 2020
Oct
02

Protein phosphorylation can induce signal transduction to change sperm motility patterns during sperm capacitation. However, cha

 
Oct 02 2020
Oct
02

Chloroviruses produce a capsid protein containing N-linked glycans differing in structure from those found in all other organism

 
Oct 02 2020
Oct
01

Objectives: Diets may alter an individual's metabolism and inflammation, collectively leading to the modulation of cardiovascula

 
Oct 01 2020
Sep
30
TRIM28 Regulates Dlk1 Expression in Adipogenesis
Int J Mol Sci. 2020 Sep 30; 21(19):7245.
Ching-Jin Chang, Sheng-Wei Lin

The tripartite motif-containing protein 28 (TRIM28) is a transcription corepressor, interacting with histone deacetylase and met

 
Sep 30 2020
Sep
28
Preparation and characterization of antibody-drug conjugates acting on HER2-positive cancer cells
PLoS One. 2020 Sep 28; 15(9):e0239813. eCollection 2020.
Andrew H-J Wang

Two systems of antibody-drug conjugates (ADCs), noncleavable H32-DM1 and cleavable H32-VCMMAE, were developed by using different

 
Sep 28 2020
Sep
25

In protein engineering and synthetic biology, Methanosarcina mazei pyrrolysyl-tRNA synthetase (MmPy

 
Sep 25 2020
Sep
25

Tracking DNA double strand break (DSB) repair is paramount for the understanding and therapeutic development of various diseases

 
Sep 25 2020
Sep
21

Overcoming resistance to bioactive small molecules is a significant challenge for health care and agriculture. As a result, effo

 
Sep 21 2020
Sep
18
Microcephaly family protein MCPH1 stabilizes RAD51 filaments
Nucleic Acids Res. 2020 Sep 18; 48(16):9135-9146.
Peter Chi

Microcephalin 1 (MCPH1) was identified from genetic mutations in patients with primary autosomal recessive microcephaly. In resp

 
Sep 18 2020
Sep
01
The study of isotopic enrichment of water in human plasma and erythrocyte
FASEB J. 2020 Sep; 34(9):13049-13062. Epub 2020 Aug 10.
Chiaolong Hsiao

Life does not sustain without water. For water, there is a natural abundance of stable isotope hydrogen and oxygen. Water molecu

 
Sep 01 2020
Sep
01

The undecaprenyl pyrophosphate phosphatase (UppP) is an integral membrane pyrophosphatase. In bacteria, UppP catalyzes the depho

 
Sep 01 2020
Aug
29

Chemical examination from the cultured soft coral Sarcophyton digitatum resulted in the isolation and structural identification

 
Aug 29 2020
Aug
21

We developed micro-liquid enclosure arrays (MLEAs) for holding solution samples in coherent diffractive imaging (CDI) using x-ra

 
Aug 21 2020
Aug
20
Converting Escherichia coli to a Synthetic Methylotroph Growing Solely on Methanol
Cell. 2020 Aug 20; 182(4):933-946.e14. Epub 2020 Aug 10.
James C. Liao

Methanol, being electron rich and derivable from methane or CO2, is a potentially renewable one-carbon (C1) feedstock for microo

 
Aug 20 2020
Aug
18
Aging shifts mitochondrial dynamics toward fission to promote germline stem cell loss
Aging Cell. 2020 Aug; 19(8):e13191. Epub 2020 Jul 14.
Chi-Kuang Yao, Guang-Chao Chen

Changes in mitochondrial dynamics (fusion and fission) are known to occur during stem cell differentiation; however, the role of

 
Aug 18 2020
Aug
11

The influenza virus hemagglutinin (HA) and coronavirus spike (S) protein mediate virus entry. HA and S proteins are heavily glyc

 
Aug 11 2020
Aug
04
Insights Into Dynamics of Inhibitor and Ubiquitin-Like Protein Binding in SARS-CoV-2 Papain-Like Protease
Front Mol Biosci. 2020 Aug 4; 7:174. eCollection 2020.
Kuen-Phon Wu

Covid-19 is caused by a novel form of coronavirus for which there are currently no vaccines or anti-viral drugs. This virus, ter

 
Aug 04 2020
Aug
03
Discovery of a Dual Function Cytochrome P450 that Catalyzes Enyne Formation in Cyclohexanoid Terpenoid Biosynthesis
Angew Chem Int Ed Engl. 2020 Aug 3; 59(32):13537-13541. Epub 2020 Jun 2.
Hsiao-Ching Lin, Chun-Hung Lin

The 1,3-enyne moiety is commonly found in cyclohexanoid natural products produced by endophytic and plant pathogenic fungi. Aspe

 
Aug 03 2020
Aug
01
Mycobacterium bovis BCG infection alters the macrophage N-glycome
Mol Omics. 2020 Aug 1; 16(4):345-354. Epub 2020 Apr 9.
Kay-Hooi Khoo

Macrophage glycosylation is essential to initiate the host-immune defense but may also be targeted by pathogens to promote infec

 
Aug 01 2020
Jul
30
PHRF1 promotes migration and invasion by modulating ZEB1 expression
PLoS One. 2020 Jul 30; 15(7):e0236876. eCollection 2020.
Mau-Sun Chang

PHRF1 (PHD and RING finger domain-containing protein 1) suppresses acute promyelocytic leukemia (APL) by promoting TGIF (TG-inte

 
Jul 30 2020
Jul
15
Development of a Versatile and Modular Linker for Antibody-Drug Conjugates Based on Oligonucleotide Strand Pairing
Bioconjug Chem. 2020 Jul 15; 31(7):1804-1811. Epub 2020 Jun 25.
Andrew H-J Wang

Linker design is crucial to the success of antibody-drug conjugates (ADCs). In this work, we developed a modular linker format f

 
Jul 15 2020
Jul
07
Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis
Nat Commun. 2020 Jul 7; 11(1):3396.
Todd L. Lowary