Institute of Biological Chemistry, Academia Sinica

Research

Glycobiology and Immunology

My role at IBC is to manage the Glycobiology Core Facility (GCF), which supports PIs to conduct glycobiology research by application of the existing technologies, reagents and tools. My expertise also helps facilitate multi-disciplinary collaborations with the initial focus on galectins. The future direction of GCF will be guided by the research projects of IBC PIs. To further promote the utilization of this core, more advanced technologies and platforms will be established per request by PIs.

The Galectin Core Facility is dedicated to services involving:
– Production of glycan-binding proteins (GBPs) and their antibodies; Production and maintenance GBP- and glycan-engineered mammalian cell lines.
– Establishment of analytical platform, using GBPs as glycan sensors (intra- and extra-cellular) and applying GBP engineered reporter cells and GBP array; performance of time-lapse live cell imaging and biochemical analysis of cell signaling.

Metagenomic Data analysis

My personal research interest is to perform microbiome metadata analysis and data visualization. Collaborations with IBC PIs in this emerging research field is also highly welcomed.

Degrees and Positions Held

• 2003 – 2007   Ph.D., Department of Microbiology and Molecular genetics, University of California, Irvine, CA, USA
• 2000 – 2002   M.S., Institute of Biopharmaceutical Sciences, National Yang-Ming University
• 1996 – 2000   B.S., Department of Biology, National Cheng-Kung University

• 2022 – present   Assistant Research Specialist, Glycobiology Core Facility, Institute of Biological Chemistry, Academia Sinica
• 2017 – 2021   Adjunct Assistant Professor, National Defense University
• 2016 – 2022   Project Scientist, Institute of Biomedical Sciences, Academia Sinica
• 2011 – 2015   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica
• 2008 – 2010   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica

Selected Publications

Galectin-3 promotes non-canonical inflammasome activation through intracellular binding to lipopolysaccharide glycans. 
Lo TH, Chen HL, Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT* 
Proc Natl Acad Sci USA. (2021)

Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis. 
Chen HL, Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT* 
The Journal of clinical investigation (2021)

Role of skin and gut microbiota in the pathogenesis of psoriasis, an inflammatory skin disease. 
Hsu DK, Fung MA, Chen HL* 
Medicine in Microecology (2020)

MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms. 
Huang JY, Chen HL, Shih C* 
Scientific Rep. (2016)

Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling. 
Chen HL, Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT* 
J. Invest. Dermatol. (2016)

Galectins and neuroinflammation. 
Chen HL, Liao F, Lin TN, Liu FT* 
Advances in neurobiology (2014)

MicroRNA-22 can reduce parathymosin expression in transdifferentiated hepatocytes. 
Chen HL, Huang JY, Chen CM, Chu TH, Shih C* 
PLoS one (2012)

Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis. 
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M* 
Nature communications (2011)

T cell receptor signaling co-regulates multiple Golgi genes to enhance N-glycan branching. 
Chen HL, Li CF, Grigorian A, Tian W, Demetriou M* 
The Journal of biological chemistry (2009)

Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling. 
Chen IJ, Chen HL, Demetriou M* 
The Journal of biological chemistry (2007)

Publication List