Institute of Biological Chemistry, Academia Sinica

Research

• Manager of Biophysics Core Facility (BCF), Department of Academic Affairs and Instrument Service,
  supporting BCF users of following technologies:
  1. Analytical ultracentrifugation (AUC)
  2. Bio-layer Interferometry (BLI)
  3. Capillary isoelectric focusing technology (cIEF)
  4. Circular dichroism (CD )
  5. Dynamic light scattering (DLS)
  6. Microcalorimetry, ITC & DSC
  7. MicroScale Thermophoresis (MST)
  8. Surface plasmon resonance technology (SPR)
• Protein Chemistry:

Protein over-expression using E. coli or yeast system, various purification techniques (precipitation, gel filtration, ion-exchange, hydrophobic interaction, reverse phase HPLC, affinity chromatography), electrophoresis, steady-state enzyme kinetics, site directed mutagenesis

• Computational Chemistry:

Modeling protein-protein and protein-ligand interactions, Homology modeling, and electrostatic calculations by Discovery Studio, CHARMM or pKa suite

• Web Design:

This is not much a “research interest”, only my interest.
Macromedia Dreamweaver, Fireworks, Flash, Adobe Photoshop, Illustrator, workstation administration, PHP and MySQL database management

Degrees and Positions Held

• 1993 – 1999   Ph.D., Chemistry, Case Western Reserve University
• 1993 – 1996   Master, Chemistry, Case Western Reserve University
• 1986 – 1990   Bachelor, Chemistry, National Taiwan Normal University

• 2009 – present   Associate Research Specialist, Institute of Biological Chemistry/ Scientific Instrument Center
• 2008 – 2009   Assistant Research Specialist, Inst. of Biological Chemistry / Scientific Instrument Center
• 2005 – 2008   Committee member, NMR group, Structure Analysis Committee, Scientific Instrument Center
• 2004 – 2008   Assistant Research Specialist, Inst. of Biological Chemistry, Academia Sinica
• 2001 – 2004   Postdoctoral Fellow, Chemistry, Academia Sinica
• 1999 – 2001   Postdoctoral Research Associate, Biochemistry and Biophysics, Texas A&M University
• 1999 – 1999   Postdoctoral Research Associate, Medicinal Chemistry and Molecular Pharmacology, Purdue University
• 1994 – 1999   Research Assistant, Case Western Reserve University
• 1993 – 1994   Teaching Assistant, Case Western Reserve University
• 1990 – 1992   Chemistry and Physics Teacher, Chung-Shan Junior High

Selected Publications

The calcium-chelating capability of tetrahydrofuranic moieties modulates the cytotoxicity of annonaceous acetogenins. 
Liaw CC, Liao WY, Chen CS, Jao SC, Wu YC, Shen CN, Wu SH 
Angewandte Chemie-International Edition (2011)

Polyfluorinated bipyridine cisplatins manipulate cytotoxicity through the induction of S-G(2)/M arrest and partial intercalation mechanism. 
Chang TT, More SV, Lu N, Jhuo JW, Chen YC, Jao SC, Li WS 
Bioorganic & medicinal chemistry (2011)

Inhibition of Glutathione S-Transferase M1 by New Gabosine Analogues Is Essential for Overcoming Cisplatin Resistance in Lung Cancer Cells. 
Wang CH, Wu HT, Cheng HM, Yen TJ, Lu IH, Chang HC, Jao SC, Shing TK, Li WS 
Journal of medicinal chemistry (2011)

Overcoming the drug resistance in breast cancer cells by rational design of efficient glutathione S-transferase inhibitors. 
Li WS, Lam WS, Liu KC, Wang CH, Chang HC, Jen YC, Hsu YT, Shivatare SS, Jao SC 
Organic letters (2010)

Synthesis and structure-activity relationships of novel furazan-3,4-diamide analogs as potent anti-cancer agents. 
Li WS, More SV, Wang CH, Jen YC, Yao CF, Wang TF, Hung CC, Jao SC 
Bioorganic & medicinal chemistry letters (2010)

Evaluation of organophosphorus chemicals-degrading enzymes: a comparison of Escherichia coli and human cytosolic aminopeptidase P. 
Y.-T. Hsu, C.-Y. Su, H.-C. Du, S.-C. Jao & W.-S. Li 
Chemistry & biodiversity (2008)

Enzymatic and Nonenzymatic Synthesis of Glutathione Conjugates: Application to the Understanding of Parasite’s Defense System and Alternative to the Discovery of Potent Glutathione S-Transferase Inhibitors. 
W.-J. Lo, Y.-C. Chiou, Y.-T. Hsu, W. S. Lam, M.-Y. Chang, S.-C. Jao & W.-S. Li 
Bioconjugate Chemistry (2007)

Design of potent inhibitors for Schistosoma japonica glutathione S-transferase. 
S.-C. Jao, J. Chen, K. Yang & W.-S. Li 
Bioorganic & Medicinal Chemistry (2006)

Computational and mutational analysis of human glutaredoxin (thioltransferase): probing the molecular basis of the low pKa of cysteine 22 and its role in catalysis. 
S.-C. Jao, S. M. English Ospina, A. J. Berdis, D. W. Starke, C. B. Post & J. J. Mieyal 
Biochemistry (2006)

Aminopeptidase p mediated detoxification of organophosphonate analogues of sarin: mechanistic and stereochemical study at the phosphorus atom of the substrate. 
L.-F. Huang, B. Su, S.-C. Jao, K.-T. Liu & W.-S. Li 
Chembiochem : a European journal of chemical biology (2006)

Publication List