Institute of Biological Chemistry, Academia Sinica

Research

Specialty

1. Protein X-ray Crystallography
2. Synchrotron X-ray Data Collection
3. Structure Biology
4. Structure-based Drug Optimization
5. Structure-based Protein Analysis

Research

• Structure-based Discovery, Design and Optimization of Small Molecule Drugs for the Treatment of Neurodegenerative Diseases

Degrees and Positions Held

• 2002 – 2006   Ph.D., Institute of Biochemical Sciences, National Taiwan University
• 1991 – 1993   M.S., Institute of Biochemical Sciences, National Taiwan University
• 1986 – 1991   B.S., Department of Veterinary Medicine, National Chung-Hsing University

• 2017 – present   Senior Research Specialist, Institute of Biological Chemistry, Academia Sinica
• 2016 – present   Chief Project Manager, Taiwan Protein Projec
• 2013 – 2017   Associate Research Specialist, Institute of Biological Chemistry, Academia Sinica
• 2011 – 2015   Project Manager, Core Facilities for Protein Structural Analysis
• 2009 – 2012   Assistant Research Specialist, Institute of Biological Chemistry, Academia Sinica
• 2005 – 2011   Project Manager, Project Manager, National Core Facility for Protein Production and X‐ray Structural Analysis
• 1993 – 2009   Research Assistant, Institute of Biological Chemistry, Academia Sinica

Selected Publications

Structural and biological insights into Klebsiella pneumonia surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use. 
Tu IF, Lin TL, Yang FL, Lee IM, Tu WL, Liao JH, Ko TP, Wu WJ, Jan JT, Ho MR, Chou CY, Wang AH, Wu CY, Wang JT, Huang KF, Wu SH 
Journal of Biomedical Science (2022)

A GalNAc/Gal-specific lectin modulates immune responses via toll-like receptor 4 independently of carbohydrate-binding ability. 
Chen ST, Liao JH, Huang KF, Lee IM, Wong WT, Wu SH, Hua KF 
Chemical Communications (2021)

A Unique Carboxylic-Acid Hydrogen-Bond Network (CAHBN) Confers Glutaminyl Cyclase Activity on M28 Family Enzymes. 
Huang KF, Huang JS, Wu ML, Hsieh WL, Hsu KC, Hsu HL, Ko TP, Wang AH 
Journal of Molecular Biology (2021)

Structural basis for fragmenting the exopolysaccharide of Acinetobacter baumannii by bacteriophage ΦAB6 tailspike protein. 
Lee IM, Tu IF, Yang FL, Ko TP, Liao JH, Lin NT, Wu CY, Ren CT, Wang AH, Chang CM, Huang KF, Wu SH 
Scientific Reports (2017)

A Multivalent Marine Lectin from Crenomytilus grayanus Possesses Anti-cancer Activity through Recognizing Globotriose Gb3. 
Liao JH, Chien CT, Wu HY, Huang KF, Wang I, Ho MR, Tu IF, Lee IM, Li W, Shih YL, Wu CY, Lukyanov PA, Hsu ST, Wu SH 
Journal of the American Chemical Society (2016)

Linked Production of Pyroglutamate-Modified Proteins via Self-Cleavage of Fusion Tags with TEV Protease and Autonomous N-Terminal Cyclization with Glutaminyl Cyclase In Vivo. 
Shih YP, Chou CC, Chen YL, Huang KF, Wang AH 
PLoS One (2014)

Structural and functional analyses of a glutaminyl cyclase from Ixodes scapularis reveal metal-independent catalysis and inhibitor binding. 
Huang KF, Hsu HL, Karim S, Wang AH 
Acta Crystallographica Section D : Biological Crystallography (2014)

Structures of Human Golgi-resident Glutaminyl Cyclase and Its Complexes with Inhibitors Reveal a Large Loop Movement upon Inhibitor Binding. 
Huang KF, Liaw SS, Huang WL, Chia CY, Lo YC, Chen YL, Wang AH 
Journal of Biological Chemistry (2011)

A conserved hydrogen-bond network in the catalytic centre of animal glutaminyl cyclases is critical for catalysis. 
Huang KF, Wang YR, Chang EC, Chou TL, Wang AH 
The Biochemical journal (2008)

Crystal structures of human glutaminyl cyclase, an enzyme responsible for protein N-terminal pyroglutamate formation. 
Huang KF, Liu YL, Cheng WJ, Ko TP, Wang AH 
Proceedings of the National Academy of Sciences of the United States of America (2005)

Publication List