Institute of Biological Chemistry, Academia Sinica

Research

1. Protein Chemistry: Cell culture, Molecular cloning, Site-directed mutagenesis, Protein expression, Protein purification, Enzyme kinetic analysis.
2. Biophysical Instrument: Circular dichroism, Fluorescence spectroscopy, Analytic ultracentrifugation, Isothermal titration calorimetry, Surface plasmon resonance.

Degrees and Positions Held

• 2002 – 2007   Ph.D., Institute of Biochemical Sciences, National Taiwan University
• 1997 – 1999   M.S., Biological Science and Technology, National Chiao Tung University

• 2019 – present   Associate Research Specialist, Institute of Biological Chemistry, Academia Sinica
• 2010 – 2019   Assistant Research Specialist, Institute of Biological Chemistry, Academia Sinica
• 2007 – 2009   Postdoctoral Fellow, Genomics Research Center, Academia Sinica
• 2007 – 2007   Postdoctoral Fellow, Institute of Biological Chemistry, Academia Sinica
• 2000 – 2005   Research Assistant, Institute of Biological Chemistry, Academia Sinica

Selected Publications

Generation of TRIM28 Knockout K562 Cells by CRISPR/Cas9 Genome Editing and Characterization of TRIM28-Regulated Gene Expression in Cell Proliferation and Hemoglobin Beta Subunits. 
Chang YJ, Kang Z, Bei J, Chou SJ, Lu MJ, Su YL, Lin SW, Wang HH, Lin S, Chang CJ 
International Journal of Molecular Sciences (2022)

Preparation of Cas9 Ribonucleoproteins for Genome Editing. 
Lin SW, Nguyen VQ, Lin S 
Bio-protocol (2022)

A chemical probe inhibitor targeting STAT1 restricts cancer stem cell traits and angiogenesis in colorectal cancer. 
Chou PH, Luo CK, Wali N, Lin WY, Ng SK, Wang CH, Zhao M, Lin SW, Yang PM, Liu PJ, Shie JJ, Wei TT 
Journal of Biomedical Science (2022)

The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain. 
Hsieh HH, Chen YA, Chang YJ, Wang HH, Yu YH, Lin SW, Huang YJ, Lin S, Chang CJ 
Journal of Inflammation (2021)

Publication List