Research Specialists +886-2-27855696,4012
Room 401, IBC, AS


Experience in qualitative and quantitative proteomics (iTRAQ, SILAC, label-free) workflows by NanoLC-MS/MS analyses, in conjunction with utilizing a variety of database search engine and data handling programs for high-throughput expression profiling and quantification.

Comprehensive proteome-wide analysis of post-translational modifications, such as phosphorylation, and targeted analysis of protein sumoylation, neddylation and cysteine modifications etc.

Targeted analysis or manual examination of MS and MS/MS data to determine a true protein ID hit from false positive, to rescue false negative from the data, to confirm predicted sequence or modifications, and to determine site-specific PTM.

Degrees and Positions Held

  • 1997 – 2002   Ph.D., Chemistry, National Taiwan University
  • 2014 – present   Associate Research Specialist, Inst. of Biological Chemistry / Scientific Instrument Center
  • 2009 – 2014   Assistant Research Specialist, Inst. of Biological Chemistry / Scientific Instrument Center
  • 2003 – 2009   Postdoctoral Fellow, and R&D Manager (from 2006), NRPGM Core Facilities for Proteomic Research

Selected Publications

Suppressive regulation of KSHV RTA with O-GlcNAcylation. 
Ko YC, Tsai WH, Wang PW, Wu IL, Lin SY, Chen YL, Chen JY, Lin SF 
Journal of biomedical science (2012)

Ser-634 and Ser-636 of Kaposi’s Sarcoma-Associated Herpesvirus RTA are Involved in Transactivation and are Potential Cdk9 Phosphorylation Sites. 
Tsai WH, Wang PW, Lin SY, Wu IL, Ko YC, Chen YL, Li M, Lin SF 
Frontiers in microbiology (2012)

Structural and functional roles of Daxx SIM phosphorylation in SUMO paralog-selective binding and apoptosis modulation. 
Chang CC, Naik MT, Huang YS, Jeng JC, Liao PH, Kuo HY, Ho CC, Hsieh YL, Lin CH, Huang NJ, Naik NM, Kung CC, Lin SY, Chen RH, Chang KS, Huang TH, Shih HM 
Molecular cell (2011)

Phosphorylation of the zebrafish M6Ab at serine 263 contributes to filopodium formation in PC12 cells and neurite outgrowth in zebrafish embryos. 
Huang KY, Chen GD, Cheng CH, Liao KY, Hung CC, Chang GD, Hwang PP, Lin SY, Tsai MC, Khoo KH, Lee MT, Huang CJ 
PloS one (2011)

Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy. 
Tang HW, Wang YB, Wang SL, Wu MH, Lin SY, Chen GC 

EGFR promotes lung tumorigenesis by activating miR-7 through a Ras/ERK/Myc pathway that targets the Ets2 transcriptional repressor ERF. 
Chou YT, Lin HH, Lien YC, Wang YH, Hong CF, Kao YR, Lin SC, Chang YC, Lin SY, Chen SJ, Chen HC, Yeh SD, Wu CW 
Cancer research (2010)

Phosphoproteomics of Klebsiella pneumoniae NTUH-K2044 reveals a tight link between tyrosine phosphorylation and virulence. 
Lin MH, Hsu TL, Lin SY, Pan YJ, Jan JT, Wang JT, Khoo KH, Wu SH 

Tyrosine phosphoproteomics and identification of substrates of protein tyrosine phosphatase dPTP61F in Drosophila S2 cells by mass spectrometry-based substrate trapping strategy. 
Chang YC, Lin SY, Liang SY, Pan KT, Chou CC, Chen CH, Liao CL, Khoo KH, Meng TC 
Journal of proteome research (2008)

Precise mapping of increased sialylation pattern and the expression of acute phase proteins accompanying murine tumor progression in BALB/c mouse by integrated sera proteomics and glycomics. 
Lin SY, Chen YY, Fan YY, Lin CW, Chen ST, Wang AH, Khoo KH 
Journal of proteome research (2008)

Concerted experimental approach for sequential mapping of peptides and phosphopeptides using C18-functionalized magnetic nanoparticles. 
Hsiao HH, Hsieh HY, Chou CC, Lin SY, Wang AH, Khoo KH 
Journal of proteome research (2007)

Publication List