Principal Investigators
kkhoo@gate.sinica.edu.tw +886-2-27855696,6030
Kay-Hooi Khoo DISTINGUISHED RESEARCH FELLOW
Room 603, IBC, AS
Research
Degrees and Positions Held
Selected Publications
Publication List

Research

Capitalizing on recent rapid advances in mass spectrometry instrumentation to deliver the much needed improved sensitivity, selectivity and throughput in protein modification analysis, proteomics, glycomics and glycoproteomics, without compromising precision. The current focus areas and enabling MS-based techniques to be developed include:

  • high throughput and high sensitivity LC-MS/MS-based mapping and sequencing of complex N- and O-glycans, towards a global and targeted glycomics of sufficient breadth and depth;
  • functional sulfoglycomics, towards unraveling myriad sulfated glycotopes implicated in mediating cell-cell interactions, particularly those of human and mouse myeloid and lymphoid cells and tissues;
  • glycopeptide sequencing/identification MS data acquisition and analysis workflows, towards efficient localization of biologically implicated and/or structurally interesting glycotopes onto their protein carriers;
  • quantitative determination of site-specific Cys modifications and the dynamics of their inter-conversion, particularly those of receptor protein tyrosine phosphatase

Degrees and Positions Held

  • 2012 – present   Distinguished Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2017 – 2019   Acting Director, Institute of Biological Chemistry, Academia Sinica
  • 2012 – 2017   Associate Director, Institute of Biological Chemistry, Academia Sinica
  • 2008 – 2012   Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 1996 – 2008   Associate Research Fellow , Institute of Biological Chemistry, Academia Sinica
  • 1994 – 1996   Postdoctoral Fellow, Colorado State University,
  • 1992 – 1994   Postdoctoral Fellow , Imperial College, University of London.
  • 1989 – 1992   Ph.D. , Biochemistry, Imperial College, University of London.
  • 1986 – 1989   B.S. , Biochemistry, Imperial College, University of London.

Selected Publications

High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha. 
Chien YC, Wang YS, Sridharan D, Kuo CW, Chien CT, Uchihashi T, Kato K, Angata T, Meng TC, Hsu STD and Khoo KH 
JACS Au (2023)

Distinct shifts in site-specific glycosylation pattern of SARS-CoV-2 spike proteins associated with arising mutations in the D614G and Alpha variants. 
Kuo CW, Yang TJ, Chien YC, Yu PY, Hsu SD and Khoo KH 
Glycobiology (2022)

Discovery Sulfoglycomics and Identification of the Characteristic Fragment Ions for High-Sensitivity Precise Mapping of Adult Zebrafish Brain-Specific Glycotopes. 
Tseng HC, Hsiao CT, Yamakawa N, Guérardel Y and Khoo KH 
Front Mol Biosci (2021)

A mass spectrometry-based glycotope-centric cellular glycomics is the more fruitful way forward to see the forest for the trees. 
Khoo KH 
Biochem Soc Trans (2021)

Strategic Applications of Negative-Mode LC-MS/MS Analyses to Expedite Confident Mass Spectrometry-Based Identification of Multiple Glycosylated Peptides. 
Kuo CW and Khoo KH 
Anal Chem (2020)

Cryo-EM analysis of a feline coronavirus spike protein reveals a unique structure and camouflaging glycans. 
Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW and Hsu SD 
Proc Natl Acad Sci U S A (2020)

Functional roles of ST8SIA3-mediated sialylation of striatal dopamine D2 and adenosine A2A receptors. 
Lin CY, Lai HL, Chen HM, Siew JJ, Hsiao CT, Chang HC, Liao KS, Tsai SC, Wu CY, Kitajima K, Sato C, Khoo KH and Chern Y 
Transl Psychiatry (2019)

Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns. 
Yamakawa N, Vanbeselaere J, Chang LY, Yu SY, Ducrocq L, Harduin-Lepers A, Kurata J, Aoki-Kinoshita KF, Sato C, Khoo KH, Kitajima K and Guerardel Y 
Nat Commun (2018)

Distinct substrate specificities of human GlcNAc-6-sulfotransferases revealed by mass spectrometry-based sulfoglycomic analysis. 
Yu SY, Hsiao CT, Izawa M, Yusa A, Ishida H, Nakamura S, Yagi H, Kannagi R and Khoo KH 
J Biol Chem (2018)

Advancing a High Throughput Glycotope-centric Glycomics Workflow Based on NAnoLC-MS2-product Dependent-MS3 ANAlysis of Permethylated Glycans. 
Hsiao CT, Wang PW, Chang HC, Chen YY, Wang SH, Chern Y, and Khoo KH* 
Mol Cell Proteomics (2017)

Direct Mapping of Additional Modifications on Phosphorylated O-glycans of α-Dystroglycan by Mass Spectrometry Analysis in Conjunction with Knocking Out of Causative Genes for Dystroglycanopathy. 
Yagi H, Kuo CW, Obayashi T, Ninagawa S, Khoo KH, and Kato K 
Mol Cell Proteomics (2016)

Efficient Mapping of Sulfated Glycotopes by Negative Ion Mode nanoLC-MS/MS-Based Sulfoglycomic Analysis of Permethylated Glycans. 
Cheng CW, Chou CC, Hsieh HW, Tu Z, Lin CH, Nycholat C, Fukuda M, and Khoo KH 
Anal Chem (2015)

Publication List