Principal Investigators
rhchen@as.edu.tw +886-2-27855696,6020
Ruey-Hwa Chen DISTINGUISHED RESEARCH FELLOW
Room 602, IBC, AS
Research
Degrees and Positions Held
Selected Publications
Publication List

Research

Purinosome represents a type of biomolecular condensate that consists of all six enzymes in the de novo purine synthesis (DNPS) pathway. Under several stressed conditions that enhance cell demand on purine, purinosome is formed to promote DNPS pathway flux, thereby facilitating cell adaptation. The followings are our major research directions related to purinosome. 

1. Purinosome assembly and disassembly mechanisms. 

2. Puirnosome in regulating cell metabolism and organelle functions

3. Purinosome in controlling cancer cell survival, tumor microenvironment, and therapy resistance 

4. Purinosome in regulating metabolic diseases and aging

Degrees and Positions Held

  • 1987 – 1991   Ph.D, Biochemistry, Michigan State University
  • 1983 – 1985   M.S., Biochemistry, National Taiwan University
  • 1979 – 1983   B.S., Agricultural Chemistry, National Taiwan University
  • 2012 – present   Distinguished Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2011 – 2014   Deputy Director, Institute of Biological Chemistry, Academia Sinica
  • 2006 – 2012   Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2003 – 2006   Professor, Institute of Molecular Medicine, College of Medicine, National Taiwan University
  • 1996 – 2003   Associate Professor, Institute of Molecular Medicine, College of Medicine, National Taiwan University
  • 1995 – 1996   Assistant Biochemist, University of California at San Francisco
  • 1992 – 1995   Postdoctoral Fellow, University of California at San Francisco

Selected Publications

Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies.  
Yeat NY, Liu LH, Chang YH, Lai CP, Chen RH  
Developmental Cell (2025)

PAICS ubiquitination recruits UBAP2 to trigger phase separation for purinosome assembly.  
Chou MC, Wang YH, Chen FY, Kung CY, Wu KP, Kuo JC, Chan SJ, Cheng ML, Lin CY, Chou YC, Ho MC, Firestine S, Huang Jr, Chen RH  
Molecular Cell (2023)

TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation.     
Chen YH, Chen HH, Wang WJ, Chen HY, Huang WS, Kao CH, Lee SR, Yeat NY, Yan RL, Chan SJ, Wu KP, Chen RH     
Nature Communications (2023)

VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism.     
Chen YH, Huang TY, Lin YT, Lin SY, Li WH, Hsiao HJ, Yan RL, Tang HW, Shen ZQ, Chen GC, Wu KP, Tsai TF, Chen RH     
Nature Communications (2021)

Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization.     
Chiang SY, Wu HC, Lin SY, Chen HY, Wang CF, Yeh NH, Shih JH, Huang YS, Kuo HC, Chou SJ, Chen RH     
Science Advances (2021)

Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment. (Selected as a highlight in JCI This Month)     
Wang YT, Chen J, Chang CW, Jen J, Huang TY, Chen CM, Shen R, Liang SY, Cheng IC, Yang SC, Lai WW, Cheng KH, Hsieh TS, Lai MZ, Cheng HC, Wang YC, Chen RH     
J Clin Invest (2017)

Cul3-KLHL20 Ubiquitin Ligase Governs the Turnover of ULK1 and VPS34 Complexes to Control Autophagy Termination. (Highlighted in Editor’s Corner and Selected for a ‘Punctum’ contribution in Autophagy)     
Liu CC, Lin YC, Chen YH, Chen CM, Pang LY, Chen HA, Wu PR, Lin MY, Jiang ST, Tai TF, Chen RH     
Mol Cell (2016)

K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. (selected as a Mol Cell Preview and a Research Highlight in Nature Rev Mol Cell Biol)     
Yuan WC, Lee YR, Lin SY, Chang LY, Tan YP, Hung CC, Kuo JC, Liu CH, Lin MY, Xu M, Chen ZJ, Chen RH     
Mol Cell (2014)

USP11 regulates PML stability to control Notch-induced malignancy in brain tumours.     
Wu HC, Lin YC, Liu CH, Chung HC, Wang YT, Lin YW, Ma HI, Tu PH, Lawler SE, Chen RH     
Nature Commun (2014)

A Cullin3-KLHL20 Ubiquitin Ligase-Dependent Pathway Targets PML to Potentiate HIF-1 Signaling and Prostate Cancer Progression. (recommended by Faculty of 1000 Biology as F1000 factor 9.0 “MUST READ” grade)     
Yuan WC, Lee YR, Huang SF, Lin YM, Chen TY, Chung HC, Tsai CH, Chen HY, Chiang CT, Lai CK, Lu LT, Chen CH, Gu DL, Pu YS, Jou YS, Lu KP, Hsiao PW, Shih HM, Chen RH     
Cancer cell (2011)

Publication List