Institute of Biological Chemistry, Academia Sinica
ccyu2@mx.nthu.edu.tw
03-571-5131
Our research program is focused in the areas of biosynthetic chemistry (with a particular emphasis in enzymatic carbohydrate synthesis), the structure determination/analysis of oligosaccharides and the discovery of novel carbohydrate-active enzymes that can be used in complex oligosaccharide synthesis. Students and postdoctoral fellows in the group use synthetic chemistry and enzymatic strategy to prepare compounds with interesting biological activities and then have the opportunity to use them in either biochemical or conformational studies.
The long-term goals of our research program are to develop state-of-the-art methods for assembling complex, biologically-relevant carbohydrates (glycans) that can be used to probe their function in nature. My underlying research philosophy is to work on problems in which developing methods for preparing novel, structurally-interesting glycans leads to tools for studying vital biological problems. The major area of focus is human milk glycans. Specific systems of interest are: 1) Discovering new enzymes as biocatalysts for human milk-type glycan synthesis; 2) Chemoenzymatic synthesis of the human milk oligosaccharide library; and 3) The function of human milk oligosaccharides in human gut microbiota and to investigate their roles in preventing the pathogenic infections.
Sulfo-Fluorous Tagging Strategy for Site-Selective Enzymatic Glycosylation of para-Human Milk Oligosaccharides.
Huang YT, Su YC, Wu HR, Huang HH, Lin EC, Tsai TW, Tseng HW, Fang JL, Yu CC*
ACS Catalysis (2021)
Effective Separation of Human Milk Glycosides using Carbon Dioxide Supercritical Fluid Chromatography.
Liou SW, Fang JL, Lin HW, Tsai TW, Huang HH, Liang CY, Yang CR, Wei GT*, Yu CC*
Chemistry - An Asian Journal (2021)
Substrate Characterization of Bacteroides fragilis α1,3/4-Fucosyltransferase Enabling Access to Programmable One-Pot Enzymatic Synthesis of KH 1 Antigen.
Huang HH, Fang JL, Wang HK, Sun CY, Tsai TW, Huang YT, Kuo CY, Wang YJ, Liao CC, Yu CC*
ACS Catalysis (2019)
Exploring the Synthetic Application of Helicobacter pylori α1,3/4-Fucosyltransferase FucTIII toward the Syntheses of Fucosylated Human Milk Glycans and Lewis Antigens.
Tsai TW, Fang JL, Liang CY, Wang CJ, Huang YT, Wang YJ, Li JY, Yu CC*
ACS Catalysis (2019)
Enzymatic Synthesis of Human Milk Fucosides α1,2‐Fucosyl para‐Lacto‐N‐Hexaose and its Isomeric Derivatives.
Fang JL, Tsai TW, Liang CY, Li JY, Yu CC*
Advanced Synthesis & Catalysis (2018)
Water-soluble Sulfo-Fluorous Affinity (SOFA) Tag-Assisted Enzymatic Synthesis of Oligosaccharides.
Hou KL, Chiang PY, Lin CH, Li BY, Chien WT, Huang YT, Yu CC*, Lin CC*
Advanced Synthesis & Catalysis (2018)
Recent Developments in Enzymatic Synthesis of Modified Sialic Acid Derivatives.
Yu CC, Withers SG
Advanced Synthesis & Catalysis (2015)
A Glyco-Gold Nanoparticle Based Assay for α-2,8-Polysialyltransferase from Neisseria meningitides.
Yu CC, Huang LD, Kwan D, Wakarchuk WW, Withers SG*, Lin CC*
Chemical Communications (2013)
Site-Specific Immobilization of Enzymes on Magnetic Nanoparticles and their Use in Organic Synthesis.
Yu CC, Kuo YY, Liang CF, Chien WT, Wu HT, Chang TC, Jan FD, Lin CC*
Bioconjugate Chemistry (2012)
Site-Specific Immobilization of CMP-Sialic Acid Synthetase on Magnetic Nanoparticles and its Use in the Synthesis of CMP-Sialic Acid.
Yu CC, Lin PC, Lin CC*
Chemical Communications (2008)
