Institute of Biological Chemistry, Academia Sinica
USER COMMITTEE MEMBERS
LOCATIONThe Bio-Imaging Core Facility is located at Room 416, Institute of Biological Chemistry, Academia Sinica.
For instrument education and training information please refer to https://sites.google.com/view/bicf.
This facility provides the following services for IBC researchers:
•Design of bio-imaging-related experimental methods.
•Assist in the selection of suitable imaging systems, image processing, and image analysis.
•Provide biological imaging-related application knowledge and course training.
•One-stop bio-imaging experiment and analysis services.
Imaging systems provided at this facility include:
•Laser-scanning confocal microscopes
•Fluorescence microscope
•High-content screening station
•Flow cytometers
•Bioluminescence and Chemiluminescence Imager.
Acknowledgement of the Core Facility
All users of the Imaging Core Facility should acknowledge the facility in publications and presentations that include data generated in the facility. Please also acknowledge the person who services imaging analysis services. We also appreciate receiving an electronic version of publications that include data gathered in our facility.
Acknowledgment template
We thank the Bio-Imaging Core Facility at the Institute of Biological Chemistry, Academia Sinica, for the technical support in image acquisition and analysis.
Equipped
Laser: 405, 470-670 white laser
Motorized XY Scanning Stage
Z-axis micro-vibration stage
Application
Live Cell Imaging
Functional imaging experiments
FRAP (Fluorescence recovery after photobleaching)
FRET (Fluorescence Resonance Energy Transfer)
Fluorescence Colocalization
Equipped
Laser : diode laser 405, 445, 488, 514, 561, 594 and 640
Motorized XY Scanning Stage
High Sensitivity-Spectral Detector
Resonant scanner
Silicone Objectives
Application
Live Cell Imaging
Functional imaging experiments
FRAP (Fluorescence recovery after photobleaching)
FRET (Fluorescence Resonance Energy Transfer)
Fluorescence Colocalization
Equipped
Olympus IX83 Automated inverted microscope
Application
Cell counting
Intracellular transport
Translocation
Cell proliferation
Bacterial and viral infection assays
Cell-cycle analysis
Protein localization and colocalization
Live cell assays
Fluorescence analysis in tissue sections
Cell migration
Equipped
ZEISS Axiocam 208 color camera
CoolCube 1Digital High Res. CCD Camera
Application
Image acquisition and reporting
Fluorescence analysis in tissue sections
Karyotyping
Equipped
Trans-illumination white light and UV
Epi-illumination white light, red, green and blue fluorescence.
Application
Chemiluminescence imaging
Fluorescence imaging
Equipped
9.1 megapixel cooled 16-bit CCD camera
Invitrogen Smart Exposure technology
Advanced automated features
Green LED-based transilluminator
Application
Protein gel and membranes
Nucleic acid gel
Chemiluminescence imaging
Fluorescence imaging
Equipped
Laser : 405,488,638nm
8 channels for fluorescence detection
Application
Immunophenotyping
Absolute counting
Residual white blood cell enumeration
Stem cell analysis
DNA analysis
Application
Image acquisition and reporting
Fluorescence analysis in tissue sections
‘SRRF-Stream+’ is a real-time super-resolution microscopy functionality that super-charges the imaging performance of your standard microscope.
Application
Image acquisition and reporting
Fluorescence analysis in tissue sections
Olympus SZX16 with DP80 acquire bright-field and fluorescence images of the same field. Meanwhile, the slim LED transmitted light illuminator provides brightfield, contrast-enhancing oblique and darkfield illumination, it performance superior quality image for research purpose.
Equipped
Laser : diode laser 405, 488, 561, and 640
Motorized XY Scanning Stage
GaAsp ultra-high sensitivity sensor
32 channel matrix GaAsp sensor
10x Plan-Apochromat objective
20x Plan-Apochromat objective
40x LD C-Apochromat Water objective
63x Plan-Apochromat Oil objective
DIC observation kit
Application
Super Resolution (AiryScan)
Colocalization
3D imaging
Time-lapse imaging,Photo Bleaching (FRAP, FLIP, FRET, Photoactivation, Photoconversion)
Linear unmixing
CONTACT
lunkung@gate.sinica.edu.tw 
+886-2-27855696,4160