Institute of Biological Chemistry, Academia Sinica
USER COMMITTEE MEMBERS
LOCATION
CONTACT
ycl@gate.sinica.edu.tw 
+886-2-27855696,4100Mammalian cell-based recombinant protein expression facility provides a sterile environment and instruments (Biosafety Cabinet, Carbon Dioxide Incubators, Cell Counters, Water bath, Temperature-Controlled Centrifuge and microscopes) of cell culture for scientists in gene transfection and protein production. The facility specializes in mammalian and insect cells expression systems. These systems supply the expression and modification requirements for a wide variety of proteins.
Thermo 370 CO2 Incubators which offer high-temperature decontamination cycle and a unique in-chamber HEPA air filtration system. This incubator is use for culturing with 35mm, 100mm dishes or 25T, 75T flasks.
Thermo 3950 Incubators, equipped with two shakers, is use for large scale protein expression in mammalian cells. Two incubation condition can be selected, one is 37℃, 8% CO2 and the other is 32℃, 8% CO2.
Thermo MaxQ6000 Incubators is use for protein expression in insect cells.
These safety cabinets, in accordance with NSF/ANSI 49, offer operator and environment protection.
The Countess 3 automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.
The Leica DM IL LED Fluorescence Inverted Microscope is ideal for cell culture and routine live cell examinations.
1. Available for 4X, 10x, 20x, and 40x magnification.
2. High-intensity, high-contrast 5W LED illumination.
3. Equipped with a 3 position fluorescence slider (405, FITC, TRITC).
4.CANON camera.
The J6-MI centrifuge works with JS-4.2A rotor, max speed is 4200 RPM. It is used for separation of protein production and cells.