Mammalian cell-based recombinant protein expression
Ying-Chu Lee +886-2-27855696,4100

Mammalian cell-based recombinant protein expression facility provides a sterile environment and instruments (Biosafety Cabinet, Carbon Dioxide Incubators, Cell Counters, Water bath, Temperature-Controlled Centrifuge and microscopes) of cell culture for scientists in gene transfection and protein production. The facility specializes in mammalian and insect cells expression systems. These systems supply the expression and modification requirements for a wide variety of proteins.

1. Thermo Scientific™ Forma™ Steri-Cycle™ CO2 Incubators TYPE: Thermo 370

Thermo 370 CO2 Incubators which offer high-temperature decontamination cycle and a unique in-chamber HEPA air filtration system. This incubator is use for culturing with 35mm, 100mm dishes or 25T, 75T flasks.

2. Thermo Scientific Large Capacity CO2 Incubator TYPE: Thermo 3950

Thermo 3950 Incubators, equipped with two shakers, is use for large scale protein expression in mammalian cells. Two incubation condition can be selected, one is 37℃, 8% CO2 and the other is 32℃, 8% CO2.

3. Thermo Scientific™ MaxQ™ 6000 Incubated/Refrigerated Stackable Shakers TYPE: Thermo MaxQ6000

Thermo MaxQ6000 Incubators is use for protein expression in insect cells.

4. Class II, Type A2 Biological Safety Cabinet TYPE: Thermo 1377

These safety cabinets, in accordance with NSF/ANSI 49, offer operator and environment protection.

5. The Countess 3 automated cell counter TYPE: Countess 3

The Countess 3 automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

6. Fluorescence Inverted Microscope TYPE: Leica DM IL LED Fluorescence Inverted Microscope

The Leica DM IL LED Fluorescence Inverted Microscope is ideal for cell culture and routine live cell examinations.
1. Available for 4X, 10x, 20x, and 40x magnification.
2. High-intensity, high-contrast 5W LED illumination.
3. Equipped with a 3 position fluorescence slider (405, FITC, TRITC).
4.CANON camera.

7. Centrifuge TYPE: Beckman Coulter J6-MI

The J6-MI centrifuge works with JS-4.2A rotor, max speed is 4200 RPM. It is used for separation of protein production and cells.