研究領域與專長

Structure-based Protein Functions

This research is focused on developing structure-based system biological science, under the Taiwan Protein Project, since January 2016. We have been engaged in the technical development of SACLA, the Japanese X-ray Free Electron Laser, as well as protein microcrystallography using the SPring-8 beamline. These technological resources will enable the determination of huge complex structures and the time series structures of protein and nucleic acid molecules.

The biological aim of the research is to accomplish the complete elucidation of the intracellular subsystems of life, through structural and functional analyses. We are focusing on the “protein-synthesis (translation)” and “DNA-repair” systems, as essential cellular processes in the central dogma of life. We will also elucidate the mechanisms of disease-relevant proteins, from a structural chemistry viewpoint. Mitochondrial diseases are particularly advanced in the project, since heteroplasmic defects of mitochondrial protein synthesis are their underlying causes in many cases.

This is an intimate research collaboration between the RIKEN SPring-8 Center, Japan, and the Academia Sinica, Taiwan.

學經歷
經歷
  • 2016 – 迄今   Visiting Professor, Institute of Biological Chemistry, Academia Sinica, Taiwan
  • 2013 – 2015   Visiting Expert, Institute of Physics, Academia Sinica, Taiwan
  • 2009 – 2013   Team Leader, Functomics Biology II Research Team, SR System Biology Research Group, RIKEN SPring-8 Center.
  • 2008 – 2009   Senior Scientist, Systems and Structural Biology Center, RIKEN Yokohama Institute.
  • 2004 – 2007   Senior Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2001 – 2003   Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2000 – 2001   Post-doctoral Researcher, New York State University.
  • 1996 – 1999   Assistant Professor (Research Associate), Division of Biological Science, Graduate School of Science, Nagoya University.
  • 1992 – 1996   Research Associate, Division of Biological Science, Nagoya University.
學歷
  • 1997   Ph. D., Biology, Nagoya University, Japan
  • 1991   M. Sc., Biology, Nagoya University, Japan
  • 1989   B. Sc., Biology, Shizuoka University, Japan
主要著作
Twist and turn: a revised structural view on the unpaired bubble of class II CPD photolyase in complex with damaged DNA.
Maestre-Reyna M, Yamamoto J, Huang WC, Tsai MD, Essen LO, Bessho Y
IUCrJ. (2018)
Imaging live cell in micro-liquid enclosure by X-ray laser diffraction.
Kimura T, Joti Y, Shibuya A, Song C, Kim S, Tono K, Yabashi M, Tamakoshi M, Moriya T, Oshima T, Ishikawa T, Bessho Y, and Nishino Y
Nat Commun. (2014)
Crystal structure of putative CbiT from Methanocaldococcus jannaschii: an intermediate enzyme activity in cobalamin (vitamin B12) biosynthesis.
Padmanabhan B, Yokoyama S, and Bessho Y
BMC Struct Biol. (2013)
Life without tRNAArg-adenosine deaminase TadA: evolutionary consequences on decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes.
Yokobori S, Kitamura A, Grosjean H, and Bessho Y
Nucleic Acids Res. (2013)
Characterization and structure of the Aquifex aeolicus protein DUF752: a bacterial tRNA-methyltransferase (MnmC2) functioning without the usually fused oxidase domain (MnmC1).
Kitamura A, Nishimoto M, Sengoku T, Shibata R, Jäger G, Björk GR, Grosjean H, Yokoyama S, and Bessho Y
J Biol Chem. (2012)
Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli.
Kitamura A, Sengoku T, Nishimoto M, Yokoyama S, and Bessho Y
Protein Sci. (2011)
Structural basis for functional mimicry of long-variable-arm tRNA by tmRNA.
Bessho Y, Shibata R, Sekine SI, Murayama K, Higashijima K, Hori-Takemoto C, Shirouzu M, Kuramitsu S, and Yokoyama S
Proc Natl Acad Sci U S A. (2007)
A tRNA aminoacylation system for non-natural amino acids based on a programmable ribozyme.
Bessho Y, Hodgson DR, and Suga H
Nat Biotechnol. (2002)
Planarian mitochondria sequence heterogeneity: Relationships between the type of cytochrome c oxidase subunit I gene sequence, karyotype and genital organ.
Bessho Y, Tamura S, Hori H, Tanaka H, Ohama T, and Osawa S
Mol Ecol. (1997)
Release factors and genetic code.
Jukes TH, Bessho Y, Ohama T, and Osawa S
Nature (1991)
著作列表

Publications list

Structure-based Protein Functions

This research is focused on developing structure-based system biological science, under the Taiwan Protein Project, since January 2016. We have been engaged in the technical development of SACLA, the Japanese X-ray Free Electron Laser, as well as protein microcrystallography using the SPring-8 beamline. These technological resources will enable the determination of huge complex structures and the time series structures of protein and nucleic acid molecules.

The biological aim of the research is to accomplish the complete elucidation of the intracellular subsystems of life, through structural and functional analyses. We are focusing on the “protein-synthesis (translation)” and “DNA-repair” systems, as essential cellular processes in the central dogma of life. We will also elucidate the mechanisms of disease-relevant proteins, from a structural chemistry viewpoint. Mitochondrial diseases are particularly advanced in the project, since heteroplasmic defects of mitochondrial protein synthesis are their underlying causes in many cases.

This is an intimate research collaboration between the RIKEN SPring-8 Center, Japan, and the Academia Sinica, Taiwan.

經歷
  • 2016 – 迄今   Visiting Professor, Institute of Biological Chemistry, Academia Sinica, Taiwan
  • 2013 – 2015   Visiting Expert, Institute of Physics, Academia Sinica, Taiwan
  • 2009 – 2013   Team Leader, Functomics Biology II Research Team, SR System Biology Research Group, RIKEN SPring-8 Center.
  • 2008 – 2009   Senior Scientist, Systems and Structural Biology Center, RIKEN Yokohama Institute.
  • 2004 – 2007   Senior Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2001 – 2003   Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2000 – 2001   Post-doctoral Researcher, New York State University.
  • 1996 – 1999   Assistant Professor (Research Associate), Division of Biological Science, Graduate School of Science, Nagoya University.
  • 1992 – 1996   Research Associate, Division of Biological Science, Nagoya University.
學歷
  • 1997   Ph. D., Biology, Nagoya University, Japan
  • 1991   M. Sc., Biology, Nagoya University, Japan
  • 1989   B. Sc., Biology, Shizuoka University, Japan
Twist and turn: a revised structural view on the unpaired bubble of class II CPD photolyase in complex with damaged DNA.
Maestre-Reyna M, Yamamoto J, Huang WC, Tsai MD, Essen LO, Bessho Y
IUCrJ. (2018)
Imaging live cell in micro-liquid enclosure by X-ray laser diffraction.
Kimura T, Joti Y, Shibuya A, Song C, Kim S, Tono K, Yabashi M, Tamakoshi M, Moriya T, Oshima T, Ishikawa T, Bessho Y, and Nishino Y
Nat Commun. (2014)
Crystal structure of putative CbiT from Methanocaldococcus jannaschii: an intermediate enzyme activity in cobalamin (vitamin B12) biosynthesis.
Padmanabhan B, Yokoyama S, and Bessho Y
BMC Struct Biol. (2013)
Life without tRNAArg-adenosine deaminase TadA: evolutionary consequences on decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes.
Yokobori S, Kitamura A, Grosjean H, and Bessho Y
Nucleic Acids Res. (2013)
Characterization and structure of the Aquifex aeolicus protein DUF752: a bacterial tRNA-methyltransferase (MnmC2) functioning without the usually fused oxidase domain (MnmC1).
Kitamura A, Nishimoto M, Sengoku T, Shibata R, Jäger G, Björk GR, Grosjean H, Yokoyama S, and Bessho Y
J Biol Chem. (2012)
Crystal structure of the bifunctional tRNA modification enzyme MnmC from Escherichia coli.
Kitamura A, Sengoku T, Nishimoto M, Yokoyama S, and Bessho Y
Protein Sci. (2011)
Structural basis for functional mimicry of long-variable-arm tRNA by tmRNA.
Bessho Y, Shibata R, Sekine SI, Murayama K, Higashijima K, Hori-Takemoto C, Shirouzu M, Kuramitsu S, and Yokoyama S
Proc Natl Acad Sci U S A. (2007)
A tRNA aminoacylation system for non-natural amino acids based on a programmable ribozyme.
Bessho Y, Hodgson DR, and Suga H
Nat Biotechnol. (2002)
Planarian mitochondria sequence heterogeneity: Relationships between the type of cytochrome c oxidase subunit I gene sequence, karyotype and genital organ.
Bessho Y, Tamura S, Hori H, Tanaka H, Ohama T, and Osawa S
Mol Ecol. (1997)
Release factors and genetic code.
Jukes TH, Bessho Y, Ohama T, and Osawa S
Nature (1991)

Publications list