研究領域與專長

組蛋白(histone)的轉譯後修飾可以調控DNA跟其他蛋白質的結合,因此在各種跟DNA相關的生物途徑(如轉錄跟DNA複製)中扮演重要的角色。這些轉譯後修飾包括磷酸化、泛素化、ADP-ribosylation、O-GlcNAcylation、甲基化和乙醯化。組蛋白乙醯轉移酶(histone acetyltransferase, HAT)可以將組蛋白乙醯化,而且近年來發現,除了組蛋白,HAT也可以乙醯化許多其他蛋白質,顯示HAT的功能比過去所想的更為複雜。我們實驗室對了解HAT的功能以及其中的機制很感興趣,而目前我們主要專注於一個果蠅的HAT:Enok。Enok對neuroblast增生以及幹細胞維持很重要,而且是另一個HAT MOZ的同源蛋白;MOZ是人體造血作用的主要調控蛋白,也參與白血病的致病機制。基於Enok和MOZ之間的高度相似性,我們對利用果蠅這個模式生物來研究Enok/MOZ的調控及功能很有興趣。

目前,我們主要致力於兩個方向:

  1. 了解Enok如何經由乙醯化其他蛋白質來調控細胞週期。
  2. 研究Enok在維持生殖幹細胞中的功能及機制。
學經歷
經歷
  • 2015 – 迄今   助研究員, 中央研究院生物化學研究所
  • 2011 – 2015   博士後研究員, 斯托瓦斯醫學研究所
學歷
  • 2005 – 2011   博士, 生化所, 范德堡大學醫學院
  • 2001 – 2005   學士, 生命科學系, 國立清華大學
主要著作
Huang F, Abmayr SM, Workman JL
Mol Cell Biol (2016)
Huang F, Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL
Genes Dev (2016)
Huang F, Ramakrishnan S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Bhaskara S, Cairns BR, Chandrasekharan MB
J Biol Chem (2015)
Huang F, Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL
Genes Dev (2014)
Huang F, Workman JL
Cell Res (2013)
Chandrasekharan MB, Huang F, Sun ZW
Methods (2011)
Chandrasekharan MB, Huang F, Chen YC, Sun ZW
Mol Cell Biol (2010)
Chandrasekharan MB, Huang F, Sun ZW
Epigenetics (2010)
Huang F, Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW
J Biol Chem (2010)
Chandrasekharan MB, Huang F, Sun ZW
Proc Natl Acad Sci U S A (2009)
著作列表
  1. Rastegari E, Kajal K, Tan BS, (Huang F), Chen RH, Hsieh TS, Hsu HJ  (2020-01)  Development  147(2), dev182063  "WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila."
  2. Lai CM, Lin KY, Kao SH, Chen YN, (Huang F), Hsu HJ  (2017-05)  The Journal of cell biology  216(5), 1439-1453  "Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion."
  3. (Huang F), Abmayr SM, Workman JL  (2016-06)  Molecular and cellular biology  36(14), 1900-1907  "Regulation of KAT6 Acetyltransferases and Their Roles in Cell Cycle Progression, Stem Cell Maintenance, and Human Disease."
  4. (Huang F), Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL  (2016-05)  Genes & development  30(10), 1198-1210  "The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition."
  5. (Huang F), Abmayr SM, Workman JL  (2016)  Cell Cycle  15(22), 3001-3002  "Limiting PCNA-unloading at the G1/S transition."
  6. (Huang F), Ramakrishnan S, Pokhrel S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Cairns BR, Bhaskara S, Chandrasekharan MB  (2015)  The Journal of biological chemistry  290(48), 28760-28777  "Interaction of the Jhd2 Histone H3 Lys-4 Demethylase with Chromatin Is Controlled by Histone H2A Surfaces and Restricted by H2B Ubiquitination."
  7. (Huang F), Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL  (2014)  Genes & development  28(24), 2750-2763  "Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire."
  8. (Huang F), Workman JL  (2013)  Cell research  23(10), 1153-1154  "Directing transcription to the right way."
  9. Chandrasekharan MB, (Huang F), Sun ZW  (2011)  Methods  54(3), 304-314  "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors."
  10. Chandrasekharan MB, (Huang F), Chen YC, Sun ZW  (2010)  Molecular and cellular biology  30(13), 3216-3232  "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination."
  11. Chandrasekharan MB, (Huang F), Sun ZW  (2010)  Epigenetics  5(6), 460-468  "Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation."
  12. (Huang F), Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW  (2010)  The Journal of biological chemistry  285(32), 24548-24561  "The JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation."
  13. Chandrasekharan MB, (Huang F), Sun ZW  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(39), 16686-16691  "Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability."
  14. Chen WY, Lee WC, Hsu NC, (Huang F), Chung BC  (2004)  The Journal of biological chemistry  279(37), 38730-38735  "SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1)."

組蛋白(histone)的轉譯後修飾可以調控DNA跟其他蛋白質的結合,因此在各種跟DNA相關的生物途徑(如轉錄跟DNA複製)中扮演重要的角色。這些轉譯後修飾包括磷酸化、泛素化、ADP-ribosylation、O-GlcNAcylation、甲基化和乙醯化。組蛋白乙醯轉移酶(histone acetyltransferase, HAT)可以將組蛋白乙醯化,而且近年來發現,除了組蛋白,HAT也可以乙醯化許多其他蛋白質,顯示HAT的功能比過去所想的更為複雜。我們實驗室對了解HAT的功能以及其中的機制很感興趣,而目前我們主要專注於一個果蠅的HAT:Enok。Enok對neuroblast增生以及幹細胞維持很重要,而且是另一個HAT MOZ的同源蛋白;MOZ是人體造血作用的主要調控蛋白,也參與白血病的致病機制。基於Enok和MOZ之間的高度相似性,我們對利用果蠅這個模式生物來研究Enok/MOZ的調控及功能很有興趣。

目前,我們主要致力於兩個方向:

  1. 了解Enok如何經由乙醯化其他蛋白質來調控細胞週期。
  2. 研究Enok在維持生殖幹細胞中的功能及機制。
經歷
  • 2015 – 迄今   助研究員, 中央研究院生物化學研究所
  • 2011 – 2015   博士後研究員, 斯托瓦斯醫學研究所
學歷
  • 2005 – 2011   博士, 生化所, 范德堡大學醫學院
  • 2001 – 2005   學士, 生命科學系, 國立清華大學
Huang F, Abmayr SM, Workman JL
Mol Cell Biol (2016)
Huang F, Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL
Genes Dev (2016)
Huang F, Ramakrishnan S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Bhaskara S, Cairns BR, Chandrasekharan MB
J Biol Chem (2015)
Huang F, Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL
Genes Dev (2014)
Huang F, Workman JL
Cell Res (2013)
Chandrasekharan MB, Huang F, Sun ZW
Methods (2011)
Chandrasekharan MB, Huang F, Chen YC, Sun ZW
Mol Cell Biol (2010)
Chandrasekharan MB, Huang F, Sun ZW
Epigenetics (2010)
Huang F, Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW
J Biol Chem (2010)
Chandrasekharan MB, Huang F, Sun ZW
Proc Natl Acad Sci U S A (2009)
  1. Rastegari E, Kajal K, Tan BS, (Huang F), Chen RH, Hsieh TS, Hsu HJ  (2020-01)  Development  147(2), dev182063  "WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila."
  2. Lai CM, Lin KY, Kao SH, Chen YN, (Huang F), Hsu HJ  (2017-05)  The Journal of cell biology  216(5), 1439-1453  "Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion."
  3. (Huang F), Abmayr SM, Workman JL  (2016-06)  Molecular and cellular biology  36(14), 1900-1907  "Regulation of KAT6 Acetyltransferases and Their Roles in Cell Cycle Progression, Stem Cell Maintenance, and Human Disease."
  4. (Huang F), Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL  (2016-05)  Genes & development  30(10), 1198-1210  "The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition."
  5. (Huang F), Abmayr SM, Workman JL  (2016)  Cell Cycle  15(22), 3001-3002  "Limiting PCNA-unloading at the G1/S transition."
  6. (Huang F), Ramakrishnan S, Pokhrel S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Cairns BR, Bhaskara S, Chandrasekharan MB  (2015)  The Journal of biological chemistry  290(48), 28760-28777  "Interaction of the Jhd2 Histone H3 Lys-4 Demethylase with Chromatin Is Controlled by Histone H2A Surfaces and Restricted by H2B Ubiquitination."
  7. (Huang F), Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL  (2014)  Genes & development  28(24), 2750-2763  "Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire."
  8. (Huang F), Workman JL  (2013)  Cell research  23(10), 1153-1154  "Directing transcription to the right way."
  9. Chandrasekharan MB, (Huang F), Sun ZW  (2011)  Methods  54(3), 304-314  "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors."
  10. Chandrasekharan MB, (Huang F), Chen YC, Sun ZW  (2010)  Molecular and cellular biology  30(13), 3216-3232  "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination."
  11. Chandrasekharan MB, (Huang F), Sun ZW  (2010)  Epigenetics  5(6), 460-468  "Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation."
  12. (Huang F), Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW  (2010)  The Journal of biological chemistry  285(32), 24548-24561  "The JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation."
  13. Chandrasekharan MB, (Huang F), Sun ZW  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(39), 16686-16691  "Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability."
  14. Chen WY, Lee WC, Hsu NC, (Huang F), Chung BC  (2004)  The Journal of biological chemistry  279(37), 38730-38735  "SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1)."