唾液酸隸屬於酸性糖中的一個家族，大量而廣泛的表現在所有脊索動物和部份的細菌。我們實驗室的主要研究目標分為兩方面:

1. 1. 了解唾液酸和其結合蛋白的交互作用如何調控哺乳動物的生理機能。
   2. 利用糖類生物功能的知識，致力於人體健康的提升。

目前我們的研究聚焦在唾液酸結合蛋白家族(Siglecs) ，這類蛋白大多表現在免疫系統。最近我們發現部份Siglecs 的DNA序列多樣性，會影響人類疾病與臨床病徵。現在我們正設法更進一步探究 Siglecs的分子與生理功能，並找出其基因序列多樣性和人類疾病的關聯性。遠程目標是希望應用我們的研究成果。轉而發展出唾液酸相關的疾病診斷與臨床治療方法。

經歷

• 2013 – 迄今   副研究員, 中研院生化所
• 2011 – 2013   Team Leader, RIKEN (Japan)
• 2009 – 2011   Specially Appointed Associate Professor, Osaka University (Japan)
• 2003 – 2009   Research Scientist, National Institute of Advanced Industrial Science and Technology (Japan)
• 1998 – 2003   Post-doctoral fellow, University of California at San Diego (USA)

學歷

• 1998   博士, 生物物理與生物化學系, 科學研究所, 東京大學
• 1995   碩士, 生物物理與生物化學系, 科學研究所, 東京大學
• 1993   學士, 生物物理與生物化學系, 科學學院, 東京大學

1. Li PJ, Kawade SK, Adak AK, Shen YJ, Fan CY, Hsieh YH, (Angata T), Chen YJ, Lin CC  (2022-06 accepted)  Journal of the Chinese Chemical Society  "Ligand-assisted imprinting-probe-labeling strategy reveals Siglec-7 - glycoprotein interactions"
   
2. Anwar MT, Adak AK, Kawade SK, Wu HR, (Angata T), Lin CC  (2022-05 accepted)  Bioorganic & medicinal chemistry  "Combining CuAAC reaction enables sialylated Bi- and triantennary pseudo mannose N-glycans for investigating Siglec-7 interactions."
   
3. Wu JL, Wu HY, Wu SJ, Tsai HY, Weng SH, Lin KT, Lin LI, Yao CY, Zamanova M, Lee YY, (Angata T), Tien HF, Chen YJ, Lin KI  (2022-05 accepted)  Molecular cancer research  "Phosphoproteomics Reveals the Role of Constitutive KAP1 Phosphorylation by B-Cell Receptor Signaling in Chronic Lymphocytic Leukemia."
   
4. Chang LY, Liang SY, Lu SC, Tseng HC, Tsai HY, Tang CJ, Sugata M, Chen YJ, Chen YJ, Wu SJ, Lin KI, Khoo KH, (Angata T)*  (2022-05)  Frontiers in immunology  13, 840388  "Molecular Basis and Role of Siglec-7 Ligand Expression on Chronic Lymphocytic Leukemia B Cells."
   
5. Anwar MT, Kawade SK, Huo YR, Adak AK, Sridharan D, Kuo YT, Fan CY, Wu HR, Lee YS, (Angata T)*, Lin CC*  (2022-03)  European journal of medicinal chemistry  232, 114146  "Sugar nucleotide regeneration system for the synthesis of Bi- and triantennary N-glycans and exploring their activities against siglecs."
   
6. Gebreyesus ST, Siyal AA, Kitata RB, Chen ES, Enkhbayar B, (Angata T), Lin KI, Chen YJ*, Tu HL*  (2022-01)  Nature communications  13(1), 37  "Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry."
   
7. Jiang HS, Zhuang SC, Lam CH, Chang LY, (Angata T)*  (2021-12)  Frontiers in immunology  12, 813082  "Recent Progress in the Methodologies to Identify Physiological Ligands of Siglecs."
   
8. Yoshimura A, Asahina Y, Chang LY, (Angata T), Tanaka H, Kitajima K, Sato C  (2021-02)  Journal of Biological Chemistry  296, 100477  "Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells."
   
9. Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto N, (Angata T), Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, Tsubata T  (2021-01)  Journal of Autoimmunity  116, 102571  "A Guillain-Barré syndrome-associated SIGLEC10 rare variant impairs its recognition of gangliosides."
   
10. Hsu YW, Hsu FF, Chiang MT, Tsai DL, Li FA, (Angata T), Crocker PR, Chau LY  (2021-01)  J Biomed Sci.  28, 5  "Siglec-E retards atherosclerosis by inhibiting CD36-mediated foam cell formation."
    
11. Chang LY, Low PY, Sridharan D, Gerlovin K, (Angata T)*  (2020-04)  Methods in molecular biology  2132, 85-98  "Preparation of Recombinant Siglecs and Identification of Their Ligands."
    
12. (Angata T)*  (2020-03)  Advances in experimental medicine and biology  1204, 215-230  "Siglecs that associate with DAP12."
    
13. (Angata T)*  (2020-01)  Journal of biomedical science  27(1), 10  "Siglec-15: a potential regulator of osteoporosis, cancer, and infectious diseases."
    
14. Liu CH, Raj S, Chen CH, Hung KH, Chou CT, Chen IH, Chien JT, Lin IY, Yang SY, (Angata T), Tsai WC, Wei JC, Tzeng IS, Hung SC, Lin KI*  (2019-12)  The Journal of clinical investigation  129(12), 5357-5373  "HLA-B27-mediated activation of TNAP phosphatase promotes pathogenic syndesmophyte formation in ankylosing spondylitis."
    
15. Fan CY, Hou YR, Adak AK, Waniwan JT, dela Rosa MAC, Low PY, (Angata T), Hwang KC, Chen YJ*, Lin CC*  (2019-10)  Chemical Science  10(37), 8600-8609  "Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies."
    
16. Wu HR, Anwar MT, Fan CY, Low PY, (Angata T)*, Lin CC*  (2019-10)  European journal of medicinal chemistry  180, 627-636  "Expedient assembly of Oligo-LacNAcs by a sugar nucleotide regeneration system: Finding the role of tandem LacNAc and sialic acid position towards siglec binding."
    
17. Li PJ, Huang SY, Chiang PY, Fan CY, Guo LJ, Wu DY, (Angata T), Lin CC*  (2019-08)  Angewandte Chemie International Edition  58(33), 11273-11278  "Chemoenzymatic Synthesis of DSGb5 and Sialylated Globo-series Glycans."
    
18. (Angata T)  (2018-12)  Frontiers in immunology  9, 2885  "Possible Influences of Endogenous and Exogenous Ligands on the Evolution of Human Siglecs."
    
19. Huang PJ, Low PY, Wang I, Hsu SD, (Angata T)*  (2018-12)  The Journal of biological chemistry  293(51), 19645-19658  "Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responses."
    
20. Ota F, Hirayama T, Kizuka Y, Yamaguchi Y, Fujinawa R, Nagata M, Ismanto HS, Lepenies B, Aretz J, Rademacher C, Seeberger PH, (Angata T), Kitazume S, Yoshida K, Betsuyaku T, Kida K, Yamasaki S, Taniguchi N  (2018-07)  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS  1862(7), 1592-1601  "High affinity sugar ligands of C-type lectin receptor langerin."
    
21. Hayakawa T*, Khedri Z, Schwarz F, Landig C, Liang SY, Yu H, Chen X, Fujito NT, Satta Y, Varki A, (Angata T)*  (2017-11)  BMC evolutionary biology  17(1), 228  "Coevolution of Siglec-11 and Siglec-16 via gene conversion in primates."
    
22. Wu JL, Chiang MF, Hsu PH, Tsai DY, Hung KH, Wang YH, (Angata T)*, Lin KI*  (2017-11)  Nature communications  8(1), 1854  "O-GlcNAcylation is required for B cell homeostasis and antibody responses."
    
23. Chang L, Chen YJ, Fan CY, Tang CJ, Chen YH, Low PY, Ventura A, Lin CC, Chen YJ, (Angata T)*  (2017-10)  Journal of proteome research  16, 3929-3941  "Identification of Siglec Ligands Using a Proximity Labeling Method."
    
24. (Angata T*)  (2017-10-25)  Journal of Japanese Biochemical Society  89(5), 652-659  "Polymorphisms and mutations in SIGLEC genes and their associations with diseases."
    
25. Ishii T, (Angata T), Wan ES, Cho MH, Motegi T, Gao C, Ohtsubo K, Kitazume S, Gemma A, ParE PD, Lomas DA, Silverman EK, Taniguchi N, Kida K  (2017-05)  Respirology  22(4), 684-690  "Influence of SIGLEC9 polymorphisms on COPD phenotypes including exacerbation frequency."
    
26. Graustein AD, Horne DJ, Fong JJ, Schwarz F, Mefford HC, Peterson GJ, Wells RD, Musvosvi M, Shey M, Hanekom WA, Hatherill M, Scriba TJ, Thuong NTT, Mai NTH, Caws M, Bang ND, Dunstan SJ, Thwaites GE, Varki A, (Angata T), Hawn TR  (2017-05)  Tuberculosis  104, 38-45  "The SIGLEC14 null allele is associated with Mycobacterium tuberculosis- and BCG-induced clinical and immunologic outcomes."
    
27. Toyoda M, Kaji H, Sawaki H, Togayachi A, (Angata T), Narimatsu H, Kameyama A  (2016-12)  Glycoconjugate journal  33(6), 917-926  "Identification and characterization of sulfated glycoproteins from small cell lung carcinoma cells assisted by management of molecular charges."
    
28. Fong JJ, Sreedhara K, Deng L, Varki NM, (Angata T), Liu Q, Nizet V, Varki A  (2015-11)  The EMBO journal  34(22), 2775-2788  "Immunomodulatory activity of extracellular Hsp70 mediated via paired receptors Siglec-5 and Siglec-14."
    
29. (Angata T), Nycholat CM, Macauley MS  (2015-10)  Trends in pharmacological sciences  36(10), 645-660  "Therapeutic Targeting of Siglecs using Antibody- and Glycan-Based Approaches."
    
30. Kameda Y, Takahata M, Mikuni S, Shimizu T, Hamano H, (Angata T), Hatakeyama S, Kinjo M, Iwasaki N  (2015-02)  Bone  71, 217-226  "Siglec-15 is a potential therapeutic target for postmenopausal osteoporosis."
    
31. (Angata T)*  (2014-09)  Glycobiology  24(9), 785-793  "Associations of genetic polymorphisms of Siglecs with human diseases."
    
32. (Angata T)*, Ishii T, Gao C, Ohtsubo K, Kitazume S, Gemma A, Kida K, Taniguchi N  (2014-07)  Physiological reports  2(7), e12069  "Association of serum interleukin-27 with the exacerbation of chronic obstructive pulmonary disease."
    
33. Ali SR, Fong JJ, Carlin AF, Busch TD, Linden R, (Angata T), Areschoug T, Parast M, Varki N, Murray J, Nizet V, Varki A  (2014-06)  The Journal of experimental medicine  211(6), 1231-1242  "Siglec-5 and Siglec-14 are polymorphic paired receptors that modulate neutrophil and amnion signaling responses to group B Streptococcus."
    
34. Padler-Karavani V, Hurtado-Ziola N, Chang YC, Sonnenburg JL, Ronaghy A, Yu H, Verhagen A, Nizet V, Chen X, Varki N, Varki A, (Angata T)  (2014-03)  FASEB journal  28(3), 1280-1293  "Rapid evolution of binding specificities and expression patterns of inhibitory CD33-related Siglecs in primates."
    
35. Kobayashi S, Fujinawa R, Ota F, Kobayashi S, (Angata T), Ueno M, Maeno T, Kitazume S, Yoshida K, Ishii T, Gao C, Ohtsubo K, Yamaguchi Y, Betsuyaku T, Kida K, Taniguchi N  (2013-12)  American journal of respiratory cell and molecular biology  49(6), 971-977  "A Single Dose of LPS into Mice with Emphysema Mimics Human COPD Exacerbation as Assessed by Micro-CT."
    
36. Shirato K, Gao C, Ota F, (Angata T), Shogomori H, Ohtsubo K, Yoshida K, Lepenies B, Taniguchi N  (2013)  Biochemical and biophysical research communications  435(3), 460-465  "Flagellin/Toll-like receptor 5 response was specifically attenuated by keratan sulfate disaccharide via decreased EGFR phosphorylation in normal human bronchial epithelial cells."
    
37. Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, (Angata T), Chiba Y, Ozaki H, Hirabayashi J, Tanaka Y, Mizokami M, Ikehara Y, Narimatsu H  (2013)  Journal of proteome research  12(6), 2630-2640  "Glycoproteomic discovery of serological biomarker candidates for HCV/HBV infection-associated liver fibrosis and hepatocellular carcinoma."
    
38. (Angata T), Ishii T, Motegi T, Oka R, Taylor RE, Soto PC, Chang YC, Secundino I, Gao CX, Ohtsubo K, Kitazume S, Nizet V, Varki A, Gemma A, Kida K, Taniguchi N  (2013)  Cellular and molecular life sciences : CMLS  70(17), 3199-3210  "Loss of Siglec-14 reduces the risk of chronic obstructive pulmonary disease exacerbation."
    
39. Nakajima K, Ito E, Ohtsubo K, Shirato K, Takamiya R, Kitazume S, (Angata T), Taniguchi N  (2013)  Molecular & cellular proteomics : MCP  12(9), 2468-2480  "Mass isotopomer analysis of metabolically labeled nucleotide sugars and N- and O-glycans for tracing nucleotide sugar metabolisms."
    
40. Harada Y, Nakajima K, Masahara-Negishi Y, Freeze HH, (Angata T), Taniguchi N, Suzuki T  (2013)  Proceedings of the National Academy of Sciences of the United States of America  110(48), 19366-193671  "Metabolically programmed quality control system for dolichol-linked oligosaccharides."
    
41. Kameda Y, Takahata M, Komatsu M, Mikuni S, Hatakeyama S, Shimizu T, (Angata T), Kinjo M, Minami A, Iwasaki N  (2013)  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research  28(12), 2463-2475  "Siglec-15 regulates osteoclast differentiation by modulating RANKL-induced phosphatidylinositol 3-kinase/Akt and Erk pathways in association with signaling adaptor DAP12."
    
42. Takamiya R, Ohtsubo K, Takamatsu S, Taniguchi N, (Angata T)  (2013)  Glycobiology  23(2), 178-187  "The interaction between Siglec-15 and tumor-associated sialyl-Tn antigen enhances TGF-beta secretion from monocytes/macrophages through the DAP12-Syk pathway."
    
43. Kamio K, Yoshida T, Gao C, Ishii T, Ota F, Motegi T, Kobayashi S, Fujinawa R, Ohtsubo K, Kitazume S, (Angata T), Azuma A, Gemma A, Nishimura M, Betsuyaku T, Kida K, Taniguchi N  (2012)  Biochemical and biophysical research communications  424(1), 112-117  "alpha1,6-Fucosyltransferase (Fut8) is implicated in vulnerability to elastase-induced emphysema in mice and a possible non-invasive predictive marker for disease progression and exacerbations in chronic obstructive pulmonary disease (COPD)."
    
44. Wang X, Mitra N, Cruz P, Deng L, Varki N, (Angata T), Green ED, Mullikin J, Hayakawa T, Varki A  (2012)  Molecular biology and evolution  29(8), 2073-2086  "Evolution of siglec-11 and siglec-16 genes in hominins."
    
45. (Angata T), Fujinawa R, Kurimoto A, Nakajima K, Kato M, Takamatsu S, Korekane H, Gao CX, Ohtsubo K, Kitazume S, Taniguchi N  (2012)  Annals of the New York Academy of Sciences  1253, 159-169  "Integrated approach toward the discovery of glyco-biomarkers of inflammation-related diseases."
    
46. Yasui K, (Angata T), Matsuyama N, Furuta RA, Kimura T, Okazaki H, Tani Y, Nakano S, Narimatsu H, Hirayama F  (2011)  British journal of haematology  153(6), 794-796  "Detection of anti-Siglec-14 alloantibodies in blood components implicated in nonhaemolytic transfusion reactions."
    
47. Shirato K, Nakajima K, Korekane H, Takamatsu S, Gao C, (Angata T), Ohtsubo K, Taniguchi N  (2011)  Journal of clinical biochemistry and nutrition  48(1), 20-25  "Hypoxic regulation of glycosylation via the N-acetylglucosamine cycle."
    
48. Kuno A, Ikehara Y, Tanaka Y, (Angata T), Unno S, Sogabe M, Ozaki H, Ito K, Hirabayashi J, Mizokami M, Narimatsu H  (2011)  Clinical chemistry  57(1), 48-56  "Multilectin assay for detecting fibrosis-specific glyco-alteration by means of lectin microarray."
    
49. Mitra N, Banda K, Altheide TK, Schaffer L, Johnson-Pais TL, Beuten J, Leach RJ, (Angata T), Varki N, Varki A  (2011)  The Journal of biological chemistry  286(26), 23003-23011  "SIGLEC12, a human-specific segregating (pseudo)gene, encodes a signaling molecule expressed in prostate carcinomas."
    
50. Nakajima K, Kitazume S, (Angata T), Fujinawa R, Ohtsubo K, Miyoshi E, Taniguchi N  (2010)  Glycobiology  20(7), 865-871  "Simultaneous determination of nucleotide sugars with ion-pair reversed-phase HPLC."
    
51. Yamanaka M, Kato Y, (Angata T), Narimatsu H  (2009)  Glycobiology  19(8), 841-846  "Deletion polymorphism of SIGLEC14 and its functional implications."
    
52. Mitsunaga K, Harada-Itadani J, Shikanai T, Tateno H, Ikehara Y, Hirabayashi J, Narimatsu H, (Angata T)  (2009)  Journal of biochemistry  146(3), 369-373  "Human C21orf63 is a heparin-binding protein."
    
53. Ito H, Kuno A, Sawaki H, Sogabe M, Ozaki H, Tanaka Y, Mizokami M, Shoda J, (Angata T), Sato T, Hirabayashi J, Ikehara Y, Narimatsu H  (2009)  Journal of proteome research  8(3), 1358-1367  "Strategy for glycoproteomics: identification of glyco-alteration using multiple glycan profiling tools."
    
54. Tateno H, Mori A, Uchiyama N, Yabe R, Iwaki J, Shikanai T, (Angata T), Narimatsu H, Hirabayashi J  (2008)  Glycobiology  18(10), 789-798  "Glycoconjugate microarray based on an evanescent-field fluorescence-assisted detection principle for investigation of glycan-binding proteins."
    
55. Zhang M, (Angata T), Cho JY, Miller M, Broide DH, Varki A  (2007)  Blood  109(10), 4280-4287  "Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils."
    
56. (Angata T), Tabuchi Y, Nakamura K, Nakamura M  (2007)  Glycobiology  17(8), 838-846  "Siglec-15: an immune system Siglec conserved throughout vertebrate evolution."
    
57. (Angata T), Hayakawa T, Yamanaka M, Varki A, Nakamura M  (2006)  FASEB journal : official publication of the Federation of American Societies for Experimental Biology  20(12), 1964-1973  "Discovery of Siglec-14, a novel sialic acid receptor undergoing concerted evolution with Siglec-5 in primates."
    
58. (Angata T)  (2006)  Molecular diversity  10(4), 555-566  "Molecular diversity and evolution of the Siglec family of cell-surface lectins."
    
59. Varki A, (Angata T)  (2006)  Glycobiology  16(1), 1R-27R  "Siglecs--the major subfamily of I-type lectins."
    
60. Kameyama A, Nakaya S, Ito H, Kikuchi N, (Angata T), Nakamura M, Ishida HK, Narimatsu H  (2006)  Journal of proteome research  5(4), 808-814  "Strategy for simulation of CID spectra of N-linked oligosaccharides toward glycomics."
    
61. Hayakawa T, (Angata T), Lewis AL, Mikkelsen TS, Varki NM, Varki A  (2005)  SCIENCE  309(5741), 1693  "A human-specific gene in microglia."
    
62. (Angata T), Margulies EH, Green ED, Varki A  (2004)  Proceedings of the National Academy of Sciences of the United States of America  101(36), 13251-13256  "Large-scale sequencing of the CD33-related Siglec gene cluster in five mammalian species reveals rapid evolution by multiple mechanisms."
    
63. Brinkman-Van der Linden EC, (Angata T), Reynolds SA, Powell LD, Hedrick SM, Varki A  (2003)  Molecular and cellular biology  23(12), 4199-4206  "CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice."
    
64. (Angata T), Varki A  (2002)  Chemical reviews  102(2), 439-469  "Chemical diversity in the sialic acids and related alpha-keto acids: an evolutionary perspective."
    
65. (Angata T), Kerr SC, Greaves DR, Varki NM, Crocker PR, Varki A  (2002)  The Journal of biological chemistry  277(27), 24466-24474  "Cloning and characterization of human Siglec-11. A recently evolved signaling molecule that can interact with SHP-1 and SHP-2 and is expressed by tissue macrophages, including brain microglia."
    
66. (Angata T), Brinkman-Van der Linden E  (2002)  BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS  1572(2-3), 294-316  "I-type lectins."
    
67. (Angata T), Varki NM, Varki A  (2001)  The Journal of biological chemistry  276(43), 40282-40287  "A second uniquely human mutation affecting sialic acid biology."
    
68. (Angata T), Hingorani R, Varki NM, Varki A  (2001)  The Journal of biological chemistry  276(48), 45128-45136  "Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters."
    
69. (Angata T), Varki A  (2000)  The Journal of biological chemistry  275(29), 22127-22135  "Cloning, characterization, and phylogenetic analysis of siglec-9, a new member of the CD33-related group of siglecs. Evidence for co-evolution with sialic acid synthesis pathways."
    
70. (Angata T), Varki A  (2000)  Glycobiology  10(4), 431-438  "Siglec-7: a sialic acid-binding lectin of the immunoglobulin superfamily."
    
71. (Angata T), Nakata D, Matsuda T, Kitajima K, Troy FA 2nd  (1999)  The Journal of biological chemistry  274(33), 22949-22956  "Biosynthesis of KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid). Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis."
    
72. (Angata T), Nakata D, Matsuda T, Kitajima K  (1999)  Biochemical and biophysical research communications  261(2), 326-331  "Elevated expression of free deaminoneuraminic acid in mammalian cells cultured in mannose-rich media."
    
73. (Angata T), Matsuda T, Kitajima K  (1998)  Glycobiology  8(3), 277-284  "Synthesis of neoglycoconjugates containing deaminated neuraminic acid (KDN) using rat liver alpha2,6-sialyltransferase."
    
74. (Angata T), Kitazume S, Terada T, Kitajima K, Inoue S, Troy FA 2nd, Inoue Y  (1994)  Glycoconjugate journal  11(5), 493-499  "Identification, characterization, and developmental expression of a novel alpha 2-->8-KDN-transferase which terminates elongation of alpha 2-->8-linked oligo-polysialic acid chain synthesis in trout egg polysialoglycoproteins."
    
75. (Angata T), Kitajima K, Inoue S, Chang J, Warner TG, Troy FA 2nd, Inoue Y  (1994)  Glycobiology  4(4), 517-523  "Identification, developmental expression and tissue distribution of deaminoneuraminate hydrolase (KDNase) activity in rainbow trout."