研究領域與專長

個體發育過程中神經網路是否正確的特化及連結是影響神經系統功能是否正常的關鍵,不正常的神經網路特化及連結會導致病變甚至個體的死亡。利用斑馬魚間腦的habenula nuclei (HA) 及其連結至中腦interpeduncular nucleus (IPN) 之迴路為脊椎動物的模式(圖1),我的研究在探討神經元在胚胎發育過程中如何創生及如何與其他神經元作正確的連結。利用如資料檢視、分子操控及雷射蝕損等方法, 我的結果顯示出有表現Neuropilin 1a (Nrp1a) 的HA神經元其軸突在發育過程中會隨著Semaphorin 3D (Sema3D) 分子的引導而伸入IPN 的背部區塊與其中的神經元產生連結 (圖2)。使用化學物誘導突變及原位雜交篩選等實驗策略,我的結果顯示出一個剛發現不久位在Golgi上的蛋白質Wntless (Wls) 是透過控制 neurogenin1 (ngn1) 的表現來影響HA神經元的創生(圖3)。

目前我的研究是進一步的探討在胚胎發育過程中HA神經元的軸突如何正確的長向其目標的機轉, Wls蛋白質如何控制HA神經元創生的機轉, 以及不同特性的HA神經元是如何獲得各自本身獨有的細胞特性。了解控制HA-IPN神經迴路在斑馬魚中發育的機轉將對了解此脊椎動物的腦部結構如何產生有所幫助,進而對了解與神經元創生和連結異常有關的人類病變也將有所幫助。

學經歷
經歷
  • 2018 – 迄今   合聘副研究員, 中央研究院生物化學所
  • 2016 – 迄今   副教授, 臺大生化科學所
  • 2009 – 2018   合聘助研究員, 中央研究院生物化學所
  • 2009 – 2016   助理教授, 臺大生化科學所
  • 2003 – 2008   Post-doctoral fellow, Embryology Dept., Carnegie Inst. for Science.
  • 1994 – 1996   Research assistant, Inst. of Biomedical Sciences, Academia Sinica
學歷
  • 1997 – 2003   博士, 生物系, 美國北卡羅萊納州立大學
  • 1990 – 1994   學士, 生物系, 東海大學
主要著作
Wu BT, Wen SH, Hwang SP, Huang CJ, Kuan YS
Journal of cell science (2015)
Lu CH, Lin KH, Hsu YY, Tsen KT, Kuan YS
Journal of Physics D-Applied Physics (2014)
Liao WH, Cheng CH, Hung KS, Chiu WT, Chen GD, Hwang PP, Hwang SP, Kuan YS, Huang CJ
Cellular and molecular life sciences : CMLS (2013)
Kuan, Y.S., Brewer-Jensen, P., Bai, W.L., Hunter, C., Wilson, C.B., Bass, S., Abernethy, J., Wing, J.S. and Lillie L. Searles.
Molecular and cellular biology (2009)
Kuan YS, Yu HH, Moens CB, Halpern ME
Development (2007)
Kuan YS, Gamse JT, Schreiber AM, Halpern ME
Journal of experimental zoology. Part B. Molecular and developmental evolution (2007)
著作列表
  1. Wu BT, Wen SH, Hwang SP, Huang CJ, (Kuan YS)  (2015-06)  Journal of cell science  128(12), 2328-2339  "Control of Wnt5b secretion by Wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression."
  2. Chieh-Han Lu, Kung-Hsuan Lin, Yung-Yuan Hsu, Kong-Thon Tsen and (Yung-Shu Kuan)  (2014)  Journal of Physics D-Applied Physics  47(31), 315402  "Inhibition of Escherichia coli respiratory enzymes by short visible femtosecond laser irradiation."
  3. Liao WH, Cheng CH, Hung KS, Chiu WT, Chen GD, Hwang PP, Hwang SP, (Kuan YS), Huang CJ  (2013-07)  Cellular and molecular life sciences : CMLS  70(13), 2367-2381  "Protein tyrosine phosphatase receptor type O (Ptpro) regulates cerebellar formation during zebrafish development through modulating Fgf signaling."
  4. Kuan, Y.S., Brewer-Jensen, P., Bai, W.L., Hunter, C., Wilson, C.B., Bass, S., Abernethy, J., Wing, J.S. and Lillie L. Searles.  (2009-10)  Molecular and cellular biology  29(20), 5590-603  "Drosophila Suppressor of sable protein (Su(s)) promotes degradation of aberrant and transposon-derived RNAs."
  5. Kuan YS, Yu HH, Moens CB, Halpern ME  (2007)  Development  134(5), 857-65  "Neuropilin asymmetry mediates a left-right difference in habenular connectivity."
  6. Kuan YS, Gamse JT, Schreiber AM, Halpern ME  (2007)  Journal of experimental zoology. Part B. Molecular and developmental evolution  308(5), 669-78  "Selective asymmetry in a conserved forebrain to midbrain projection."
  7. Gamse JT, Kuan YS, Macurak M, Brosamle C, Thisse B, Thisse C, Halpern ME  (2005)  Development  132(21), 4869-81  "Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target."
  8. Kuan YS, Brewer-Jensen P, Searles LL  (2004)  Molecular and cellular biology  24(9), 3734-46  "Suppressor of sable, a putative RNA-processing protein, functions at the level of transcription."
  9. Lomasney JW, Cheng HF, Wang LP, Kuan Y, Liu S, Fesik SW, King K  (1996)  The Journal of biological chemistry  271(41), 25316-26  "Phosphatidylinositol 4,5-bisphosphate binding to the pleckstrin homology domain of phospholipase C-delta1 enhances enzyme activity."
  10. Wang LP, Lim C, Kuan Y, Chen CL, Chen HF, King K  (1996)  The Journal of biological chemistry  271(40), 24505-16  "Positive charge at position 549 is essential for phosphatidylinositol 4,5-bisphosphate-hydrolyzing but not phosphatidylinositol-hydrolyzing activities of human phospholipase C delta1."

個體發育過程中神經網路是否正確的特化及連結是影響神經系統功能是否正常的關鍵,不正常的神經網路特化及連結會導致病變甚至個體的死亡。利用斑馬魚間腦的habenula nuclei (HA) 及其連結至中腦interpeduncular nucleus (IPN) 之迴路為脊椎動物的模式(圖1),我的研究在探討神經元在胚胎發育過程中如何創生及如何與其他神經元作正確的連結。利用如資料檢視、分子操控及雷射蝕損等方法, 我的結果顯示出有表現Neuropilin 1a (Nrp1a) 的HA神經元其軸突在發育過程中會隨著Semaphorin 3D (Sema3D) 分子的引導而伸入IPN 的背部區塊與其中的神經元產生連結 (圖2)。使用化學物誘導突變及原位雜交篩選等實驗策略,我的結果顯示出一個剛發現不久位在Golgi上的蛋白質Wntless (Wls) 是透過控制 neurogenin1 (ngn1) 的表現來影響HA神經元的創生(圖3)。

目前我的研究是進一步的探討在胚胎發育過程中HA神經元的軸突如何正確的長向其目標的機轉, Wls蛋白質如何控制HA神經元創生的機轉, 以及不同特性的HA神經元是如何獲得各自本身獨有的細胞特性。了解控制HA-IPN神經迴路在斑馬魚中發育的機轉將對了解此脊椎動物的腦部結構如何產生有所幫助,進而對了解與神經元創生和連結異常有關的人類病變也將有所幫助。

經歷
  • 2018 – 迄今   合聘副研究員, 中央研究院生物化學所
  • 2016 – 迄今   副教授, 臺大生化科學所
  • 2009 – 2018   合聘助研究員, 中央研究院生物化學所
  • 2009 – 2016   助理教授, 臺大生化科學所
  • 2003 – 2008   Post-doctoral fellow, Embryology Dept., Carnegie Inst. for Science.
  • 1994 – 1996   Research assistant, Inst. of Biomedical Sciences, Academia Sinica
學歷
  • 1997 – 2003   博士, 生物系, 美國北卡羅萊納州立大學
  • 1990 – 1994   學士, 生物系, 東海大學
Wu BT, Wen SH, Hwang SP, Huang CJ, Kuan YS
Journal of cell science (2015)
Lu CH, Lin KH, Hsu YY, Tsen KT, Kuan YS
Journal of Physics D-Applied Physics (2014)
Liao WH, Cheng CH, Hung KS, Chiu WT, Chen GD, Hwang PP, Hwang SP, Kuan YS, Huang CJ
Cellular and molecular life sciences : CMLS (2013)
Kuan, Y.S., Brewer-Jensen, P., Bai, W.L., Hunter, C., Wilson, C.B., Bass, S., Abernethy, J., Wing, J.S. and Lillie L. Searles.
Molecular and cellular biology (2009)
Kuan YS, Yu HH, Moens CB, Halpern ME
Development (2007)
Kuan YS, Gamse JT, Schreiber AM, Halpern ME
Journal of experimental zoology. Part B. Molecular and developmental evolution (2007)
  1. Wu BT, Wen SH, Hwang SP, Huang CJ, (Kuan YS)  (2015-06)  Journal of cell science  128(12), 2328-2339  "Control of Wnt5b secretion by Wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression."
  2. Chieh-Han Lu, Kung-Hsuan Lin, Yung-Yuan Hsu, Kong-Thon Tsen and (Yung-Shu Kuan)  (2014)  Journal of Physics D-Applied Physics  47(31), 315402  "Inhibition of Escherichia coli respiratory enzymes by short visible femtosecond laser irradiation."
  3. Liao WH, Cheng CH, Hung KS, Chiu WT, Chen GD, Hwang PP, Hwang SP, (Kuan YS), Huang CJ  (2013-07)  Cellular and molecular life sciences : CMLS  70(13), 2367-2381  "Protein tyrosine phosphatase receptor type O (Ptpro) regulates cerebellar formation during zebrafish development through modulating Fgf signaling."
  4. Kuan, Y.S., Brewer-Jensen, P., Bai, W.L., Hunter, C., Wilson, C.B., Bass, S., Abernethy, J., Wing, J.S. and Lillie L. Searles.  (2009-10)  Molecular and cellular biology  29(20), 5590-603  "Drosophila Suppressor of sable protein (Su(s)) promotes degradation of aberrant and transposon-derived RNAs."
  5. Kuan YS, Yu HH, Moens CB, Halpern ME  (2007)  Development  134(5), 857-65  "Neuropilin asymmetry mediates a left-right difference in habenular connectivity."
  6. Kuan YS, Gamse JT, Schreiber AM, Halpern ME  (2007)  Journal of experimental zoology. Part B. Molecular and developmental evolution  308(5), 669-78  "Selective asymmetry in a conserved forebrain to midbrain projection."
  7. Gamse JT, Kuan YS, Macurak M, Brosamle C, Thisse B, Thisse C, Halpern ME  (2005)  Development  132(21), 4869-81  "Directional asymmetry of the zebrafish epithalamus guides dorsoventral innervation of the midbrain target."
  8. Kuan YS, Brewer-Jensen P, Searles LL  (2004)  Molecular and cellular biology  24(9), 3734-46  "Suppressor of sable, a putative RNA-processing protein, functions at the level of transcription."
  9. Lomasney JW, Cheng HF, Wang LP, Kuan Y, Liu S, Fesik SW, King K  (1996)  The Journal of biological chemistry  271(41), 25316-26  "Phosphatidylinositol 4,5-bisphosphate binding to the pleckstrin homology domain of phospholipase C-delta1 enhances enzyme activity."
  10. Wang LP, Lim C, Kuan Y, Chen CL, Chen HF, King K  (1996)  The Journal of biological chemistry  271(40), 24505-16  "Positive charge at position 549 is essential for phosphatidylinositol 4,5-bisphosphate-hydrolyzing but not phosphatidylinositol-hydrolyzing activities of human phospholipase C delta1."