研究領域與專長

人類hPuf-A/KIAA0020蛋白在結構上具有6個類似Pumilio-RNA結合區域(PUF domain),推測可結合在特定基因的3’UTR位置,進而調控特定基因的轉譯。利用抗hPuf-A的單株抗體進行免疫螢光染色去偵測hPuf-A在細胞內的分佈位置,結果發現hPuf-A主要位於細胞核仁內,只有極少量存在細胞核質,利用Actinomycin D、DRB和camptothecin (分別是RNA聚合酶I、聚合酶II以及Topoisomerase I的抑制劑)處理人類癌細胞後,hPuf-A的分佈位置會從核仁轉移至細胞核質,顯示hPuf-A的位置當DNA損傷或RNA不穩定時會呈現動態的轉變;當我們利用siRNA降解hPuf-A蛋白的表現,缺少hPuf-A蛋白表現的細胞在處理基因毒性藥物camptothecin 和UV 後有明顯死亡,大量表現hPuf-A蛋白的細胞在處理camptothecin 和UV後卻能比對照組有明顯存活的情形,顯示hPuf-A的存在可能與基因毒性壓力的細胞反應有關;利用親和性層析與質譜分析以及免疫沉澱分析發現hPuf-A與PARP-1在同一個複合物中,PARP-1的主要功能是維繫DNA的完整性,當DNA受到損傷時PARP-1會對特定蛋白質進行poly(ADPribosyl)ation的轉譯後修飾,提高DNA修補能力,in vitro實驗發現hPuf-A會抑制PARP-1的poly(ADPribosyl)ation活性,進而影響PARP-1在細胞凋亡時被caspase切割的數量,這些結果顯示hPuf-A在RNA與DNA毒性藥物刺激的壓力反應下扮演了重要的角色。

學經歷
經歷
  • 2012 – 迄今   副教授, 臺大生化科學所
  • 2008 – 迄今   合聘助研究員, 中央研究院生物化學所
  • 2008 – 2012   助理教授, 臺大生化科學所
  • 2005 – 2008   研究員, 馬偕紀念醫院醫研部
  • 1999 – 2004   副研究員, 馬偕紀念醫院醫研部
  • 1997 – 1999   Postdoc fellow, Dana-Farber Cancer Institute, Harvard Medical School
學歷
  • 1993 – 1997   博士, 動物學研究所, 臺灣大學
  • 1992 – 1993   碩士, 動物學研究所, 臺灣大學
  • 1985 – 1989   學士, 動物系, 臺灣大學
主要著作
Chen YL, Jiang YW, Su YL, Lee SC, Chang MS, Chang CJ
Mol. Biol. Rep. (2013)
Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, Chang MS
Tumor Biol. (2013)
Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, Chang MS
PloS one (2012)
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research (2011)
Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS
Carcinogenesis (2009)
Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR
The Journal of biological chemistry (2008)
Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.
Blood (2007)
Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS
Biochemical and biophysical research communications (2006)
Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS
Cancer research (2006)
著作列表
  1. Lee JY, Fan CC, Chou NL, Lin HW, (Chang MS) (2020-07) PloS one 15(7), e0236876 “PHRF1 promotes migration and invasion by modulating ZEB1 expression.”
  2. Shih TW, Lee LJ, Chang HC, Lin HW, (Chang MS) (2020-07) Scientific reports 10(1), 10857 “An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-beta signaling.”
  3. Fan JJ, Hsu WH, Lee KH, Chen KC, Lin CW, Lee YA, Ko TP, Lee LT, Lee MT, (Chang MS), Cheng CH (2019-11) Antioxidants 8(11), E557 “Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.”
  4. Lee JY, Lee LJ, Fan CC, Chang HC, Shih HA, Min MY, (Chang MS) (2017-04) Scientific Reports 7, 45646 “Important roles of Vilse in dendritic architecture and synaptic plasticity.”
  5. Su WP, Hsu SH, Chia LC, Lin JY, Chang SB, Jiang ZD, Lin YJ, Shih MY, Chen YC, Chang MS, Yang WB, Hung JJ, Hung PC, Wu WS, Myung K, Liaw H (2016-01) Genetics 202(1), 77-92 “Combined Interactions of Plant Homeodomain and Chromodomain Regulate NuA4 Activity at DNA Double-Strand Breaks.”
  6. Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, (Chang MS)  (2015-04)  Cell death & disease  6, e1716  “PHRF1 promotes genome integrity by modulating non-homologous end-joining.”
  7. Cheng CH, Chou CM, Chu CY, Chen GD, Lien HW, Hwang PP, (Chang MS), Huang CJ  (2014)  Fish & shellfish immunology  38(1), 230-43  “Differential regulation of Tetraodon nigroviridis Mx gene promoter activity by constitutively-active forms of STAT1, STAT2, and IRF9.”
  8. Zhong W, Zhou Y, Li J, Mysore R, Luo W, Li S, (Chang MS), Olkkonen VM, Yan D  (2014)  Experimental cell research  322(2), 227-235  “OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.”
  9. Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, (Chang MS)  (2013-10)  TUMOR BIOLOGY  34(5), 2557-2564  “Upregulated hPuf-A promotes breast cancer tumorigenesis.”
  10. Chen YL, Jiang YW, Su YL, Lee SC, (Chang MS), Chang CJ  (2013-04)  Molecular biology reports  40(4), 2867-2877  “Transcriptional regulation of tristetraprolin by NF-kappaB signaling in LPS-stimulated macrophages.”
  11. Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, (Chang MS)  (2012)  PloS one  7(3), e33538  “Disruption of Murine mp29/Syf2/Ntc31 Gene Results in Embryonic Lethality with Aberrant Checkpoint Response.”
  12. Yeh PA, Yang WH, Chiang PY, Wang SC, (Chang MS), Chang CJ  (2012)  International journal of biological sciences  8(5), 606-19  “Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11.”
  13. Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, (Chang MS)  (2011-02)  Cancer research  71(3), 1126-34  “hPuf-A/KIAA0020 Modulates PARP-1 Cleavage upon Genotoxic Stress.”
  14. Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS  (2009-10)  Carcinogenesis  30(10), 1710-1716  “Involvement of p29 in DNA damage responses and Fanconi anemia pathway.”
  15. Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR  (2008)  The Journal of biological chemistry  283(4), 2454-64  “Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.”
  16. Cheng TS, Hsiao YL, Lin CC, Hsu CM, Chang MS, Lee CI, Yu RC, Huang CY, Howng SL, Hong YR  (2007)  Experimental cell research  313(8), 1710-21  “hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases.”
  17. Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.  (2007)  Blood  110(13), 4526-4534  “I branching formation in erythroid differentiation is regulated by transcription factor C/EBPα”
  18. Ho HT, Chang MS, Wei TY, Hsieh WS, Hung CC, Yang HM, Lu YT  (2006)  Chest  129(1), 95-101  “Colonization of severe acute respiratory syndrome-associated coronavirus among health-care workers screened by nasopharyngeal swab.”
  19. Chan WE, Chuang CK, Yeh SH, Chang MS, Chen SS  (2006)  Journal of virology  80(7), 3225-37  “Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus.”
  20. Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS  (2006)  Biochemical and biophysical research communications  343(2), 428-34  “Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.”
  21. Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS  (2006)  Cancer research  66(17), 8484-91  “Silencing of p29 affects DNA damage responses with UV irradiation.”
  22. Chang MS, Yang YC, Kuo YC, Kuo YH, Chang C, Chen CM, Lee TH  (2005)  Journal of natural products  68(1), 11-3  “Furocoumarin glycosides from the leaves of Ficus ruficaulis Merr. var. antaoensis.”

人類hPuf-A/KIAA0020蛋白在結構上具有6個類似Pumilio-RNA結合區域(PUF domain),推測可結合在特定基因的3’UTR位置,進而調控特定基因的轉譯。利用抗hPuf-A的單株抗體進行免疫螢光染色去偵測hPuf-A在細胞內的分佈位置,結果發現hPuf-A主要位於細胞核仁內,只有極少量存在細胞核質,利用Actinomycin D、DRB和camptothecin (分別是RNA聚合酶I、聚合酶II以及Topoisomerase I的抑制劑)處理人類癌細胞後,hPuf-A的分佈位置會從核仁轉移至細胞核質,顯示hPuf-A的位置當DNA損傷或RNA不穩定時會呈現動態的轉變;當我們利用siRNA降解hPuf-A蛋白的表現,缺少hPuf-A蛋白表現的細胞在處理基因毒性藥物camptothecin 和UV 後有明顯死亡,大量表現hPuf-A蛋白的細胞在處理camptothecin 和UV後卻能比對照組有明顯存活的情形,顯示hPuf-A的存在可能與基因毒性壓力的細胞反應有關;利用親和性層析與質譜分析以及免疫沉澱分析發現hPuf-A與PARP-1在同一個複合物中,PARP-1的主要功能是維繫DNA的完整性,當DNA受到損傷時PARP-1會對特定蛋白質進行poly(ADPribosyl)ation的轉譯後修飾,提高DNA修補能力,in vitro實驗發現hPuf-A會抑制PARP-1的poly(ADPribosyl)ation活性,進而影響PARP-1在細胞凋亡時被caspase切割的數量,這些結果顯示hPuf-A在RNA與DNA毒性藥物刺激的壓力反應下扮演了重要的角色。

經歷
  • 2012 – 迄今   副教授, 臺大生化科學所
  • 2008 – 迄今   合聘助研究員, 中央研究院生物化學所
  • 2008 – 2012   助理教授, 臺大生化科學所
  • 2005 – 2008   研究員, 馬偕紀念醫院醫研部
  • 1999 – 2004   副研究員, 馬偕紀念醫院醫研部
  • 1997 – 1999   Postdoc fellow, Dana-Farber Cancer Institute, Harvard Medical School
學歷
  • 1993 – 1997   博士, 動物學研究所, 臺灣大學
  • 1992 – 1993   碩士, 動物學研究所, 臺灣大學
  • 1985 – 1989   學士, 動物系, 臺灣大學
Chen YL, Jiang YW, Su YL, Lee SC, Chang MS, Chang CJ
Mol. Biol. Rep. (2013)
Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, Chang MS
Tumor Biol. (2013)
Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, Chang MS
PloS one (2012)
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research (2011)
Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS
Carcinogenesis (2009)
Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR
The Journal of biological chemistry (2008)
Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.
Blood (2007)
Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS
Biochemical and biophysical research communications (2006)
Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS
Cancer research (2006)
  1. Lee JY, Fan CC, Chou NL, Lin HW, (Chang MS) (2020-07) PloS one 15(7), e0236876 “PHRF1 promotes migration and invasion by modulating ZEB1 expression.”
  2. Shih TW, Lee LJ, Chang HC, Lin HW, (Chang MS) (2020-07) Scientific reports 10(1), 10857 “An important role of PHRF1 in dendritic architecture and memory formation by modulating TGF-beta signaling.”
  3. Fan JJ, Hsu WH, Lee KH, Chen KC, Lin CW, Lee YA, Ko TP, Lee LT, Lee MT, (Chang MS), Cheng CH (2019-11) Antioxidants 8(11), E557 “Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.”
  4. Lee JY, Lee LJ, Fan CC, Chang HC, Shih HA, Min MY, (Chang MS) (2017-04) Scientific Reports 7, 45646 “Important roles of Vilse in dendritic architecture and synaptic plasticity.”
  5. Su WP, Hsu SH, Chia LC, Lin JY, Chang SB, Jiang ZD, Lin YJ, Shih MY, Chen YC, Chang MS, Yang WB, Hung JJ, Hung PC, Wu WS, Myung K, Liaw H (2016-01) Genetics 202(1), 77-92 “Combined Interactions of Plant Homeodomain and Chromodomain Regulate NuA4 Activity at DNA Double-Strand Breaks.”
  6. Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, (Chang MS)  (2015-04)  Cell death & disease  6, e1716  “PHRF1 promotes genome integrity by modulating non-homologous end-joining.”
  7. Cheng CH, Chou CM, Chu CY, Chen GD, Lien HW, Hwang PP, (Chang MS), Huang CJ  (2014)  Fish & shellfish immunology  38(1), 230-43  “Differential regulation of Tetraodon nigroviridis Mx gene promoter activity by constitutively-active forms of STAT1, STAT2, and IRF9.”
  8. Zhong W, Zhou Y, Li J, Mysore R, Luo W, Li S, (Chang MS), Olkkonen VM, Yan D  (2014)  Experimental cell research  322(2), 227-235  “OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.”
  9. Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, (Chang MS)  (2013-10)  TUMOR BIOLOGY  34(5), 2557-2564  “Upregulated hPuf-A promotes breast cancer tumorigenesis.”
  10. Chen YL, Jiang YW, Su YL, Lee SC, (Chang MS), Chang CJ  (2013-04)  Molecular biology reports  40(4), 2867-2877  “Transcriptional regulation of tristetraprolin by NF-kappaB signaling in LPS-stimulated macrophages.”
  11. Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, (Chang MS)  (2012)  PloS one  7(3), e33538  “Disruption of Murine mp29/Syf2/Ntc31 Gene Results in Embryonic Lethality with Aberrant Checkpoint Response.”
  12. Yeh PA, Yang WH, Chiang PY, Wang SC, (Chang MS), Chang CJ  (2012)  International journal of biological sciences  8(5), 606-19  “Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11.”
  13. Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, (Chang MS)  (2011-02)  Cancer research  71(3), 1126-34  “hPuf-A/KIAA0020 Modulates PARP-1 Cleavage upon Genotoxic Stress.”
  14. Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS  (2009-10)  Carcinogenesis  30(10), 1710-1716  “Involvement of p29 in DNA damage responses and Fanconi anemia pathway.”
  15. Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR  (2008)  The Journal of biological chemistry  283(4), 2454-64  “Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.”
  16. Cheng TS, Hsiao YL, Lin CC, Hsu CM, Chang MS, Lee CI, Yu RC, Huang CY, Howng SL, Hong YR  (2007)  Experimental cell research  313(8), 1710-21  “hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases.”
  17. Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.  (2007)  Blood  110(13), 4526-4534  “I branching formation in erythroid differentiation is regulated by transcription factor C/EBPα”
  18. Ho HT, Chang MS, Wei TY, Hsieh WS, Hung CC, Yang HM, Lu YT  (2006)  Chest  129(1), 95-101  “Colonization of severe acute respiratory syndrome-associated coronavirus among health-care workers screened by nasopharyngeal swab.”
  19. Chan WE, Chuang CK, Yeh SH, Chang MS, Chen SS  (2006)  Journal of virology  80(7), 3225-37  “Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus.”
  20. Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS  (2006)  Biochemical and biophysical research communications  343(2), 428-34  “Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.”
  21. Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS  (2006)  Cancer research  66(17), 8484-91  “Silencing of p29 affects DNA damage responses with UV irradiation.”
  22. Chang MS, Yang YC, Kuo YC, Kuo YH, Chang C, Chen CM, Lee TH  (2005)  Journal of natural products  68(1), 11-3  “Furocoumarin glycosides from the leaves of Ficus ruficaulis Merr. var. antaoensis.”