Research

Glycobiology and Immunology

My role at IBC is to manage the Glycobiology Core Facility (GCF), which supports PIs to conduct glycobiology research by application of the existing technologies, reagents and tools. My expertise also helps facilitate multi-disciplinary collaborations with the initial focus on galectins. The future direction of GCF will be guided by the research projects of IBC PIs. To further promote the utilization of this core, more advanced technologies and platforms will be established per request by PIs.

The Galectin Core Facility is dedicated to services involving:
– Production of glycan-binding proteins (GBPs) and their antibodies; Production and maintenance GBP- and glycan-engineered mammalian cell lines.
– Establishment of analytical platform, using GBPs as glycan sensors (intra- and extra-cellular) and applying GBP engineered reporter cells and GBP array; performance of time-lapse live cell imaging and biochemical analysis of cell signaling.

Metagenomic Data analysis

My personal research interest is to perform microbiome metadata analysis and data visualization. Collaborations with IBC PIs in this emerging research field is also highly welcomed.

Degrees and Positions Held
Positions Held
  • 2022 – present   Assistant Research Specialist, Glycobiology Core Facility, Institute of Biological Chemistry, Academia Sinica
  • 2017 – 2021   Adjunct Assistant Professor, National Defense University
  • 2016 – 2022   Project Scientist, Institute of Biomedical Sciences, Academia Sinica
  • 2011 – 2015   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica
  • 2008 – 2010   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica
Degrees
  • 2003 – 2007   Ph.D., Department of Microbiology and Molecular genetics, University of California, Irvine, CA, USA
  • 2000 – 2002   M.S., Institute of Biopharmaceutical Sciences, National Yang-Ming University
  • 1996 – 2000   B.S., Department of Biology, National Cheng-Kung University
Selected Publications
Lo TH, Chen HL, Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT*
Proc Natl Acad Sci USA. (2021)
Chen HL, Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT*
The Journal of clinical investigation (2021)
Hsu DK, Fung MA, Chen HL*
Medicine in Microecology (2020)
Huang JY, Chen HL, Shih C*
Scientific Rep. (2016)
Chen HL, Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT*
J. Invest. Dermatol. (2016)
Chen HL, Liao F, Lin TN, Liu FT*
Advances in neurobiology (2014)
Chen HL, Huang JY, Chen CM, Chu TH, Shih C*
PLoS one (2012)
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M*
Nature communications (2011)
Chen HL, Li CF, Grigorian A, Tian W, Demetriou M*
The Journal of biological chemistry (2009)
Chen IJ, Chen HL, Demetriou M*
The Journal of biological chemistry (2007)
Publications List
  1. Lo TH, (Chen HL), Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT  (2021-07)  Proceedings of the National Academy of Sciences of the United States of America  118(30), e2026246118  "Galectin-3 promotes noncanonical inflammasome activation through intracellular binding to lipopolysaccharide glycans."
  2. Lei T, Blois SM, Freitag N, Bergmann M, Bhushan S, Wahle E, Huang AC, (Chen HL), Hartmann MF, Wudy SA, Liu FT, Meinhardt A, Fijak M  (2021-04)  Cellular and molecular life sciences  78(7), 3621-3635  "Targeted disruption of galectin 3 in mice delays the first wave of spermatogenesis and increases germ cell apoptosis."
  3. Lo YH, Li CS, (Chen HL), Chiang CY, Huang CC, Tu TJ, Lo TH, Choy DF, Arron JR, Chen HY, Liu FT  (2021-03)  The Journal of investigative dermatology  141(3), 503-511.e9  "Galectin-8 Is Upregulated in Keratinocytes by IL-17A and Promotes Proliferation by Regulating Mitosis in Psoriasis."
  4. (Chen HL), Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT  (2021-01)  The Journal of clinical investigation  131(1), 130740  "Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis."
  5. Hsu DK, Fung MA, (Chen HL)*  (2020-06)  Medicine in Microecology  4, 100016  "Role of skin and gut microbiota in the pathogenesis of psoriasis, an inflammatory skin disease."
  6. Hong MH, Lin WH, Weng IC, Hung YH, (Chen HL), Chen HY, Chen P, Lin CH, Yang WY, Liu FT  (2020)  Glycobiology  30(1), 46-48  "Intracellular galectins control cellular responses commensurate with cell surface carbohydrate composition."
  7. Siew JJ, Chen HM, Chen HY, (Chen HL), Chen CM, Soong BW, Wu YR, Chang CP, Chan YC, Lin CH, Liu FT, Chern Y  (2019-08)  Nature communications  10(1), 3473  "Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington\'s disease."
  8. Weng IC, (Chen HL), Lo TH, Lin WH, Chen HY, Hsu DK, Liu FT  (2018-06)  Glycobiology  28(6), 392-405  "Cytosolic galectin-3 and -8 regulate antibacterial autophagy through differential recognition of host glycans on damaged phagosomes."
  9. Huang WC, (Chen HL), Chen HY, Peng KP, Lee Y, Huang LM, Chang LY, Liu FT  (2016-12)  PloS one  11(12), e0168627  "Galectin-3 and Its Genetic Variation rs4644 Modulate Enterovirus 71 Infection."
  10. Huang JY, (Chen HL), Shih C  (2016-10)  Scientific reports  6, 34740  "MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms."
  11. (Chen HL), Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT  (2016-01)  The Journal of investigative dermatology  136(1), 182-191  "Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling."
  12. Huang JY, Chou SF, Lee JW, (Chen HL), Chen CM, Tao MH, Shih C  (2015-01)  RNA  21(3), 385-400  "MicroRNA-130a can inhibit hepatitis B virus replication via targeting PGC1alpha and PPARgamma."
  13. (Chen HL), Huang JY, Chen CM, Chu TH, Shih C  (2012-04)  PloS one  7(4), e34116  "MicroRNA-22 can reduce parathymosin expression in transdifferentiated hepatocytes."
  14. Mkhikian H, Grigorian A, Li CF, (Chen HL), Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M  (2011-05)  Nature communications  2, 334  "Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis."
  15. (Chen HL), Li CF, Grigorian A, Tian W, Demetriou M  (2009-11)  The Journal of biological chemistry  284(47), 32454-32461  "T cell receptor signaling co-regulates multiple Golgi genes to enhance N-glycan branching."
  16. Chen IJ, (Chen HL), Demetriou M  (2007-11)  The Journal of biological chemistry  282(48), 35361-35372  "Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling."
  17. (Chen HL), Lu CY, Hsu YH, Lin JJ  (2004-01)  Biochemical and biophysical research communications  313(3), 576-586  "Chromosome positional effects of gene expressions after cellular senescence."
  18. Teng SC, Epstein C, Tsai YL, Cheng HW, (Chen HL), Lin JJ  (2002-03)  Biochemical and biophysical research communications  291(3), 714-721  "Induction of global stress response in Saccharomyces cerevisiae cells lacking telomerase."

Glycobiology and Immunology

My role at IBC is to manage the Glycobiology Core Facility (GCF), which supports PIs to conduct glycobiology research by application of the existing technologies, reagents and tools. My expertise also helps facilitate multi-disciplinary collaborations with the initial focus on galectins. The future direction of GCF will be guided by the research projects of IBC PIs. To further promote the utilization of this core, more advanced technologies and platforms will be established per request by PIs.

The Galectin Core Facility is dedicated to services involving:
– Production of glycan-binding proteins (GBPs) and their antibodies; Production and maintenance GBP- and glycan-engineered mammalian cell lines.
– Establishment of analytical platform, using GBPs as glycan sensors (intra- and extra-cellular) and applying GBP engineered reporter cells and GBP array; performance of time-lapse live cell imaging and biochemical analysis of cell signaling.

Metagenomic Data analysis

My personal research interest is to perform microbiome metadata analysis and data visualization. Collaborations with IBC PIs in this emerging research field is also highly welcomed.

Positions Held
  • 2022 – present   Assistant Research Specialist, Glycobiology Core Facility, Institute of Biological Chemistry, Academia Sinica
  • 2017 – 2021   Adjunct Assistant Professor, National Defense University
  • 2016 – 2022   Project Scientist, Institute of Biomedical Sciences, Academia Sinica
  • 2011 – 2015   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica
  • 2008 – 2010   Postdoctoral Research Fellow, Institute of Biomedical Sciences, Academia Sinica
Degrees
  • 2003 – 2007   Ph.D., Department of Microbiology and Molecular genetics, University of California, Irvine, CA, USA
  • 2000 – 2002   M.S., Institute of Biopharmaceutical Sciences, National Yang-Ming University
  • 1996 – 2000   B.S., Department of Biology, National Cheng-Kung University
Lo TH, Chen HL, Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT*
Proc Natl Acad Sci USA. (2021)
Chen HL, Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT*
The Journal of clinical investigation (2021)
Hsu DK, Fung MA, Chen HL*
Medicine in Microecology (2020)
Huang JY, Chen HL, Shih C*
Scientific Rep. (2016)
Chen HL, Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT*
J. Invest. Dermatol. (2016)
Chen HL, Liao F, Lin TN, Liu FT*
Advances in neurobiology (2014)
Chen HL, Huang JY, Chen CM, Chu TH, Shih C*
PLoS one (2012)
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M*
Nature communications (2011)
Chen HL, Li CF, Grigorian A, Tian W, Demetriou M*
The Journal of biological chemistry (2009)
Chen IJ, Chen HL, Demetriou M*
The Journal of biological chemistry (2007)
  1. Lo TH, (Chen HL), Yao CI, Weng IC, Li CS, Huang CC, Chen NJ, Lin CH, Liu FT  (2021-07)  Proceedings of the National Academy of Sciences of the United States of America  118(30), e2026246118  "Galectin-3 promotes noncanonical inflammasome activation through intracellular binding to lipopolysaccharide glycans."
  2. Lei T, Blois SM, Freitag N, Bergmann M, Bhushan S, Wahle E, Huang AC, (Chen HL), Hartmann MF, Wudy SA, Liu FT, Meinhardt A, Fijak M  (2021-04)  Cellular and molecular life sciences  78(7), 3621-3635  "Targeted disruption of galectin 3 in mice delays the first wave of spermatogenesis and increases germ cell apoptosis."
  3. Lo YH, Li CS, (Chen HL), Chiang CY, Huang CC, Tu TJ, Lo TH, Choy DF, Arron JR, Chen HY, Liu FT  (2021-03)  The Journal of investigative dermatology  141(3), 503-511.e9  "Galectin-8 Is Upregulated in Keratinocytes by IL-17A and Promotes Proliferation by Regulating Mitosis in Psoriasis."
  4. (Chen HL), Lo CH, Huang CC, Lu MP, Hu PY, Chen CS, Chueh DY, Chen P, Lin TN, Lo YH, Hsiao YP, Hsu DK, Liu FT  (2021-01)  The Journal of clinical investigation  131(1), 130740  "Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis."
  5. Hsu DK, Fung MA, (Chen HL)*  (2020-06)  Medicine in Microecology  4, 100016  "Role of skin and gut microbiota in the pathogenesis of psoriasis, an inflammatory skin disease."
  6. Hong MH, Lin WH, Weng IC, Hung YH, (Chen HL), Chen HY, Chen P, Lin CH, Yang WY, Liu FT  (2020)  Glycobiology  30(1), 46-48  "Intracellular galectins control cellular responses commensurate with cell surface carbohydrate composition."
  7. Siew JJ, Chen HM, Chen HY, (Chen HL), Chen CM, Soong BW, Wu YR, Chang CP, Chan YC, Lin CH, Liu FT, Chern Y  (2019-08)  Nature communications  10(1), 3473  "Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington\'s disease."
  8. Weng IC, (Chen HL), Lo TH, Lin WH, Chen HY, Hsu DK, Liu FT  (2018-06)  Glycobiology  28(6), 392-405  "Cytosolic galectin-3 and -8 regulate antibacterial autophagy through differential recognition of host glycans on damaged phagosomes."
  9. Huang WC, (Chen HL), Chen HY, Peng KP, Lee Y, Huang LM, Chang LY, Liu FT  (2016-12)  PloS one  11(12), e0168627  "Galectin-3 and Its Genetic Variation rs4644 Modulate Enterovirus 71 Infection."
  10. Huang JY, (Chen HL), Shih C  (2016-10)  Scientific reports  6, 34740  "MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms."
  11. (Chen HL), Chiang PC, Lo CH, Lo YH, Hsu DK, Chen HY, Liu FT  (2016-01)  The Journal of investigative dermatology  136(1), 182-191  "Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling."
  12. Huang JY, Chou SF, Lee JW, (Chen HL), Chen CM, Tao MH, Shih C  (2015-01)  RNA  21(3), 385-400  "MicroRNA-130a can inhibit hepatitis B virus replication via targeting PGC1alpha and PPARgamma."
  13. (Chen HL), Huang JY, Chen CM, Chu TH, Shih C  (2012-04)  PloS one  7(4), e34116  "MicroRNA-22 can reduce parathymosin expression in transdifferentiated hepatocytes."
  14. Mkhikian H, Grigorian A, Li CF, (Chen HL), Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M  (2011-05)  Nature communications  2, 334  "Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis."
  15. (Chen HL), Li CF, Grigorian A, Tian W, Demetriou M  (2009-11)  The Journal of biological chemistry  284(47), 32454-32461  "T cell receptor signaling co-regulates multiple Golgi genes to enhance N-glycan branching."
  16. Chen IJ, (Chen HL), Demetriou M  (2007-11)  The Journal of biological chemistry  282(48), 35361-35372  "Lateral compartmentalization of T cell receptor versus CD45 by galectin-N-glycan binding and microfilaments coordinate basal and activation signaling."
  17. (Chen HL), Lu CY, Hsu YH, Lin JJ  (2004-01)  Biochemical and biophysical research communications  313(3), 576-586  "Chromosome positional effects of gene expressions after cellular senescence."
  18. Teng SC, Epstein C, Tsai YL, Cheng HW, (Chen HL), Lin JJ  (2002-03)  Biochemical and biophysical research communications  291(3), 714-721  "Induction of global stress response in Saccharomyces cerevisiae cells lacking telomerase."