Research

Structure-based Protein Functions

This research is focused on developing structure-based system biological science, under the Taiwan Protein Project, since January 2016. We have been engaged in the technical development of SACLA, the Japanese X-ray Free Electron Laser, as well as protein microcrystallography using the SPring-8 beamline. These technological resources will enable the determination of huge complex structures and the time series structures of protein and nucleic acid molecules.

The biological aim of the research is to accomplish the complete elucidation of the intracellular subsystems of life, through structural and functional analyses. We are focusing on the “protein-synthesis (translation)” and “DNA-repair” systems, as essential cellular processes in the central dogma of life. We will also elucidate the mechanisms of disease-relevant proteins, from a structural chemistry viewpoint. Mitochondrial diseases are particularly advanced in the project, since heteroplasmic defects of mitochondrial protein synthesis are their underlying causes in many cases.

This is an intimate research collaboration between the RIKEN SPring-8 Center, Japan, and the Academia Sinica, Taiwan.

Degrees and Positions Held
Positions Held
  • 2016 – Present   Visiting Professor, Institute of Biological Chemistry, Academia Sinica, Taiwan
  • 2013 – 2015   Visiting Expert, Institute of Physics, Academia Sinica, Taiwan
  • 2009 – 2013   Team Leader, Functomics Biology II Research Team, SR System Biology Research Group, RIKEN SPring-8 Center.
  • 2008 – 2009   Senior Scientist, Systems and Structural Biology Center, RIKEN Yokohama Institute.
  • 2004 – 2007   Senior Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2001 – 2003   Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2000 – 2001   Post-doctoral Researcher, New York State University.
  • 1996 – 1999   Assistant Professor (Research Associate), Division of Biological Science, Graduate School of Science, Nagoya University.
  • 1992 – 1996   Research Associate, Division of Biological Science, Nagoya University.
Degrees
  • 1997   Ph. D., Biology, Nagoya University, Japan
  • 1991   M. Sc., Biology, Nagoya University, Japan
  • 1989   B. Sc., Biology, Shizuoka University, Japan
Selected Publications
Maestre-Reyna M, Yamamoto J, Huang WC, Tsai MD, Essen LO, Bessho Y
IUCrJ. (2018)
Kimura T, Joti Y, Shibuya A, Song C, Kim S, Tono K, Yabashi M, Tamakoshi M, Moriya T, Oshima T, Ishikawa T, Bessho Y, and Nishino Y
Nat Commun. (2014)
Padmanabhan B, Yokoyama S, and Bessho Y
BMC Struct Biol. (2013)
Yokobori S, Kitamura A, Grosjean H, and Bessho Y
Nucleic Acids Res. (2013)
Kitamura A, Nishimoto M, Sengoku T, Shibata R, Jäger G, Björk GR, Grosjean H, Yokoyama S, and Bessho Y
J Biol Chem. (2012)
Kitamura A, Sengoku T, Nishimoto M, Yokoyama S, and Bessho Y
Protein Sci. (2011)
Bessho Y, Shibata R, Sekine SI, Murayama K, Higashijima K, Hori-Takemoto C, Shirouzu M, Kuramitsu S, and Yokoyama S
Proc Natl Acad Sci U S A. (2007)
Bessho Y, Hodgson DR, and Suga H
Nat Biotechnol. (2002)
Bessho Y, Tamura S, Hori H, Tanaka H, Ohama T, and Osawa S
Mol Ecol. (1997)
Jukes TH, Bessho Y, Ohama T, and Osawa S
Nature (1991)

Structure-based Protein Functions

This research is focused on developing structure-based system biological science, under the Taiwan Protein Project, since January 2016. We have been engaged in the technical development of SACLA, the Japanese X-ray Free Electron Laser, as well as protein microcrystallography using the SPring-8 beamline. These technological resources will enable the determination of huge complex structures and the time series structures of protein and nucleic acid molecules.

The biological aim of the research is to accomplish the complete elucidation of the intracellular subsystems of life, through structural and functional analyses. We are focusing on the “protein-synthesis (translation)” and “DNA-repair” systems, as essential cellular processes in the central dogma of life. We will also elucidate the mechanisms of disease-relevant proteins, from a structural chemistry viewpoint. Mitochondrial diseases are particularly advanced in the project, since heteroplasmic defects of mitochondrial protein synthesis are their underlying causes in many cases.

This is an intimate research collaboration between the RIKEN SPring-8 Center, Japan, and the Academia Sinica, Taiwan.

Positions Held
  • 2016 – Present   Visiting Professor, Institute of Biological Chemistry, Academia Sinica, Taiwan
  • 2013 – 2015   Visiting Expert, Institute of Physics, Academia Sinica, Taiwan
  • 2009 – 2013   Team Leader, Functomics Biology II Research Team, SR System Biology Research Group, RIKEN SPring-8 Center.
  • 2008 – 2009   Senior Scientist, Systems and Structural Biology Center, RIKEN Yokohama Institute.
  • 2004 – 2007   Senior Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2001 – 2003   Scientist, Genomic Sciences Center, RIKEN Yokohama Institute.
  • 2000 – 2001   Post-doctoral Researcher, New York State University.
  • 1996 – 1999   Assistant Professor (Research Associate), Division of Biological Science, Graduate School of Science, Nagoya University.
  • 1992 – 1996   Research Associate, Division of Biological Science, Nagoya University.
Degrees
  • 1997   Ph. D., Biology, Nagoya University, Japan
  • 1991   M. Sc., Biology, Nagoya University, Japan
  • 1989   B. Sc., Biology, Shizuoka University, Japan
Maestre-Reyna M, Yamamoto J, Huang WC, Tsai MD, Essen LO, Bessho Y
IUCrJ. (2018)
Kimura T, Joti Y, Shibuya A, Song C, Kim S, Tono K, Yabashi M, Tamakoshi M, Moriya T, Oshima T, Ishikawa T, Bessho Y, and Nishino Y
Nat Commun. (2014)
Padmanabhan B, Yokoyama S, and Bessho Y
BMC Struct Biol. (2013)
Yokobori S, Kitamura A, Grosjean H, and Bessho Y
Nucleic Acids Res. (2013)
Kitamura A, Nishimoto M, Sengoku T, Shibata R, Jäger G, Björk GR, Grosjean H, Yokoyama S, and Bessho Y
J Biol Chem. (2012)
Kitamura A, Sengoku T, Nishimoto M, Yokoyama S, and Bessho Y
Protein Sci. (2011)
Bessho Y, Shibata R, Sekine SI, Murayama K, Higashijima K, Hori-Takemoto C, Shirouzu M, Kuramitsu S, and Yokoyama S
Proc Natl Acad Sci U S A. (2007)
Bessho Y, Hodgson DR, and Suga H
Nat Biotechnol. (2002)
Bessho Y, Tamura S, Hori H, Tanaka H, Ohama T, and Osawa S
Mol Ecol. (1997)
Jukes TH, Bessho Y, Ohama T, and Osawa S
Nature (1991)