The CRISPR/Cas9 system is a robust genome editing tool that has revolutionized genetic studies in human cells, animals and plants. The core of technology is the enzyme Cas9, an RNA-guided DNA endonuclease that induces a double-strand break at the guide RNA-specified chromosomal locus of interest. My research focuses on developing methods to enable robust, precise and safe genome manipulations in human genome. Current projects include:
- Understanding DNA repair mechanism to enable efficient Cas9-mediated homologous recombination.
- Discovering and characterizing orthogonal CRISPR/Cas systems.
- Expanding Cas9 ribonucleoprotein nucleofection for transcription and epigenetic regulations.