The CRISPR/Cas9 system is a robust genome editing tool that has revolutionized genetic studies in human cells, animals and plants. The core of technology is the enzyme Cas9, an RNA-guided DNA endonuclease that induces a double-strand break at the guide RNA-specified chromosomal locus of interest. Our research focuses on developing methods to enable robust, precise and safe genome manipulations in human genome. Current projects include:
- Genome engineering to enhance the immune functions of human natural killer cells for immunotherapy.
- Engineering of immune cell-specific adeno-associated virus to improve DNA delivery and CRISPR gene knock-in.
- Elucidating and alleviating the cellular stresses induced by CRISPR genome editing to improve editing precision and safety.