Our laboratory has been focusing on (i) mechanistic and structural studies of prenyltransferases catalyzing chain elongation, such as undecaprenyl diphosphate synthase, octaprenyl diphosphate synthase, and geranylgeranyl diphosphate synthase; (ii) antivirus and anti-cancer drug discovery targeting 3C protease from Enterovirus 71, 3C-like proteases from SARS-CoV and MERS-CoV, neuraminidase from avian flu virus, and protein-protein interactions; (iii) multi-functional cellulose/hemicellulose degrading enzymes for biofuel production.
Our laboratory has been focusing on (i) mechanistic and structural studies of prenyltransferases catalyzing chain elongation, such as undecaprenyl diphosphate synthase, octaprenyl diphosphate synthase, and geranylgeranyl diphosphate synthase; (ii) antivirus and anti-cancer drug discovery targeting 3C protease from Enterovirus 71, 3C-like proteases from SARS-CoV and MERS-CoV, neuraminidase from avian flu virus, and protein-protein interactions; (iii) multi-functional cellulose/hemicellulose degrading enzymes for biofuel production.
Positions Held
- 2009 – present Council Member, The Taiwan society for Biochemistry and Molecular Biology
- 2007 – present Joint Professor, Insti. of Biochemical Sciences, National Taiwan University
- 2007 – present Editorial Board, Bioorganic Chemistry (Elsevier) and JCCS
- 2006 – present Research fellow, Institute of Biological Chemistry, Academia Sinica
- 2017 – 2019 Acting Deputy Director, Institute of Biological Chemistry, Academia Sinica
- 2017 – 2017 Deputy Director, Institute of Biological Chemistry, Academia Sinica
- 2010 – 2015 Council Member, Asia Pacific Protein Association (APPA)
- 2007 – 2016 Council Member, Biophysics society of ROC
- 2006 – 2011 Deputy Director, Institute of Biological Chemistry, Academia Sinica
- 2005 – 2008 PI, Core Facility of Recombinant Protein Production
- 2004 – 2011 Chem Biology & Mol Biophys Coordinator, TIGP
- 2002 – 2006 Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
- 1999 – 2002 Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica.
- 1996 – 1999 Investigator, SmithKline Beecham Pharmaceuticals.
- 1995 – 1996 Associate Research Scientist, Yale Medical School.
- 1994 – 1995 Postdoc. Pharmacology, Yale Medical School.
Degrees
- 1989 – 1994 Ph.D., Biochemistry, University of Maryland.
- 1982 – 1986 B. S., Chemistry, National Taiwan University.
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Kuo CJ, Chao TL, Kao HC, Tsai YM, Liu YK, Wang LH, Hsieh MC, Chang SY*, (Liang PH)* (2021-03) Antimicrobial Agents and Chemotherapy 65(4), e02577-20 "Kinetic characterization and inhibitor screening for the proteases leading to identification of drugs against SARS-CoV-2."
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Pathak N, Chen YT, Hsu YC, Hsu NY, Kuo CJ, Tsai HP, Kang JJ, Huang CH, Chang SY, Chang YH, (Liang PH), Yang JM (2021-01) ACS Nano 15(1), 857-872 "Uncovering Flexible Active Site Conformations of SARS-CoV-2 3CL Proteases through Protease Pharmacophore Clusters and COVID-19 Drug Repurposing."
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Chang YX, Lin YF, Chen CL, Huang MS, Hsiao M, (Liang PH)* (2020-12) Cancers 12(12), 3865 "Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways."
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Liu YJ, Chang YJ, Kuo YT, (Liang PH) (2020-05) Carcinogenesis 41(5), 699-710 "Targeting beta-tubulin/CCT-beta complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma."
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Yao JY, Teng KH, Liu MC, Wang CS, (Liang PH)* (2019-07) Molecules 24(15), 2728 "Characterization of a Cis-Prenyltransferase from Lilium longiflorum Anther"
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Chen CH, Yao JY, Yang B, Lee HL, Yuan SF, Hsieh HY, (Liang PH) (2019-02) Bioresource Technology Reports 5, 170-177 "Engineer multi-functional cellulase/xylanase/β-glucosidase with improved efficacy to degrade rice straw."
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Hsieh MH, Huang PT, Liou HH, (Liang PH), Chen PM, Holt SA, Yu IF, James M, Shiau YS, Lee MT, Lin TL, Lou KL (2018-07) Langmuir 34(30), 9036-9046 "The Penetration Depth for Hanatoxin Partitioning into the Membrane Hydrocarbon Core Measured with Neutron Reflectivity."
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Huang KH, Fang WL, Li AF, (Liang PH), Wu CW, Shyr YM, Yang MH (2018-04) International journal of surgery 52, 258-263 "Caspase-3, a key apoptotic protein, as a prognostic marker in gastric cancer after curative surgery."
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Thirumalraj B, Dhenadhayalan N, Chen SM*, Liu YJ, Chen TW, (Liang PH), Lin KC* (2018-04) Sensors & Actuators B - Chemical 259, 339–346 "Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets."
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(Liang PH), Lin WL, Hsieh HY, Lin TY, Chen CH, Tewary SK, Lee HL, Yuan SF, Yang B, Yao JY, Ho MC (2018-03) Biochimica et Biophysica Acta - General Subjects 1862(3), 513-521 "A flexible loop for mannan recognition and activity enhancement in a bifunctional glycoside hydrolase family 5."
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Liu YJ, Kumar V, Lin YF, (Liang PH) (2017-09) Cell death & disease 8(9), e3052 "Disrupting CCT-beta : beta-tubulin selectively kills CCT-beta overexpressed cancer cells through MAPKs activation."
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Kumar V, Shin JS, Shie JJ, Ku KB, Kim C, Go YY, Huang KF, Kim M, (Liang PH) (2017-05) Antiviral research 141, 101-106 "Identification and evaluation of potent Middle East respiratory syndrome coronavirus (MERS-CoV) 3CLPro inhibitors."
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Teng KH, Hsu ET, Chang YH, Lin SW, (Liang PH) (2016-08) BIOCHEMISTRY 55(31), 4366-4374 "Fluorescent Farnesyl Diphosphate Analogue: A Probe To Validate trans-Prenyltransferase Inhibitors."
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Kumar V, Tan KP, Wang YM, Lin SW, (Liang PH) (2016-07) Bioorganic & medicinal chemistry 24(13), 3035-3042 "Identification, synthesis and evaluation of SARS-CoV and MERS-CoV 3C-like protease inhibitors."
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Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, (Liang PH), Liaw YC (2015-10) Acta Crystallographica Section F-Structural Biology Communications 71(Pt 10), 1264-1272 "Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage."
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Kuo CJ, (Liang PH) (2015-04) CHEMBIOENG REVIEWS 2(2), 118–132 "Characterization and Inhibition of the Main Protease of Severe Acute Respiratory Syndrome Coronavirus."
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Yuan SF, Wu TH, Lee HL, Hsieh HY, Lin WL, Yang B, Chang CK, Li Q, Gao J, Huang CH, Ho MC, Guo RT, (Liang PH) (2015-02) The Journal of biological chemistry 290(9), 5739-5748 "Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum."
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Han X, Chen CC, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield E, Wang AH, (Liang PH), Guo RT, Ma Y (2015-01) Proteins 83(1), 37-45 "Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms."
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Kumar V, Chang CK, Tan KP, Jung YS, Chen SH, Cheng YS, (Liang PH) (2014-10) Organic letters 16(19), 5060-5063 "Identification, Synthesis, and Evaluation of New Neuraminidase Inhibitors."
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Chan HC, Feng X, Ko TP, Huang CH, Hu Y, Zheng Y, Bogue S, Nakano C, Hoshino T, Zhang L, Lv P, Liu W, Crick DC, (Liang PH), Wang AH, Oldfield E, Guo RT (2014-02) Journal of the American Chemical Society 136(7), 2892-2896 "Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis."
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Al-Mudaris ZA, Majid AS, Ji D, Al-Mudarris BA, Chen SH, (Liang PH), Osman H, Jamal Din SK, Abdul Majid AM (2013-11) PloS one 8(11), e80983 "Conjugation of benzylvanillin and benzimidazole structure improves DNA binding with enhanced antileukemic properties."
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Kumar V, Jung YS, (Liang PH) (2013-10) Expert opinion on therapeutic patents 23(10), 1337-1348 "Anti-SARS coronavirus agents: a patent review (2008 - present)."
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Chen SH, Lin SW, Lin SR, (Liang PH), Yang JM (2013-09) Journal of chemical information and modeling 53(9), 2299-2311 "Moiety-Linkage Map Reveals Selective Nonbisphosphonate Inhibitors of Human Geranylgeranyl Diphosphate Synthase."
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Lin YF, Lai TC, Chang CK, Chen CL, Huang MS, Yang CJ, Liu HG, Dong JJ, Chou YA, Teng KH, Chen SH, Tian WT, Jan YH, Hsiao M, (Liang PH) (2013-09) The Journal of clinical investigation 123(9), 3861-3875 "Targeting the XIAP/caspase-7 complex selectively kills caspase-3-deficient malignancies."
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Hwang CC, Chang YH, Lee HJ, Wang TP, Su YM, Chen HW, (Liang PH) (2013-05) PloS one 8(5), e63594 "The catalytic roles of P185 and T188 and substrate-binding loop flexibility in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni."
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Chang CK, Teng KH, Lin SW, Chang TH, (Liang PH) (2013-04) Biochemistry 52(16), 2783-2792 "Control activity of yeast geranylgeranyl diphosphate synthase from dimer interface through H-bonds and hydrophobic interaction."
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Lee HL, Chang CK, Jeng WY, Wang AH, (Liang PH) (2012-11) Protein engineering design & selection 25(11), 733-740 "Mutations in the substrate entrance region of beta-glucosidase from Trichoderma reesei improve enzyme activity and thermostability."
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Lin YF, Lee YF, (Liang PH) (2012-11) Cell Death & Disease 3, e434 "Targeting β-tubulin:CCT-β complexes incurs Hsp90 and VCP-related protein degradation and induces ER stress-associated apoptosis by triggering capacitative Ca2+ entry, mitochondrial perturbation and caspase overactivation. "
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Kuo-Hsun Teng, (Po-Huang Liang)* (2012-08) BIOORGANIC CHEMISTRY 43, 51-57 "Structures, mechanisms and inhibitors of undecaprenyl diphosphate synthase: A cis-prenyltransferase for bacterial peptidoglycan biosynthesis "
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Ren F, Ko TP, Feng X, Huang CH, Chan HC, Hu Y, Wang K, Ma Y, (Liang PH), Wang AH, Oldfield E, Guo RT. (2012-04) ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 51(17), 4157-4160 "Insights into the Mechanism of the Antibiotic-Synthesizing Enzyme MoeO5 from Crystal Structures of Different Complexes."
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Chang KM, Chen SH, Kuo CJ, Chang CK, Guo RT, Yang JM, (Liang PH). (2012-04) BIOCHEMISTRY 51(6), 3412-3419 "Roles of Amino Acids in the Escherichia coli Octaprenyl Diphosphate Synthase Active Site Probed by Structure-Guided Site-Directed Mutagenesis."
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Kesavulu MM, Tsai JY, Lee HL, (Liang PH), Hsiao CD. (2012-03) ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 68, 310-320 "Structure of the catalytic domain of the Clostridium thermocellum cellulase CelT."
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Teng KH, (Liang PH) (2012) Molecular membrane biology 29(7), 267-273 "Undecaprenyl diphosphate synthase, a cis-prenyltransferase synthesizing lipid carrier for bacterial cell wall biosynthesis."
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Teng KH, Chen AP, Kuo CJ, Li YC, Liu HG, Chen CT, (Liang PH) (2011-10) Analytical biochemistry 417(1), 136-141 "Fluorescent substrate analog for monitoring chain elongation by undecaprenyl pyrophosphate synthase in real time."
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R. Ramajayam, Kian-Pin Tan, (Po-Huang Liang)* (2011-10) BIOCHEMICAL SOCIETY TRANSACTIONS 39(5), 1371-1375 "Recent development of 3C and 3CL protease inhibitors for anti-coronavirus and anti-picornavirus drug discovery"
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Lee HL, Chang CK, Teng KH, (Liang PH) (2011-02) BIORESOURCE TECHNOLOGY 102(4), 3973-3976 "Construction and characterization of different fusion proteins between cellulases and β-glucosidase to improve glucose production and thermostability."
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Lei GS, Syu WJ, (Liang PH), Chak KF, Hu WS, Hu ST (2011-02) BMC MICROBIOLOGY 11(1), 33 "Repression of btuB gene transcription in Escherichia coli by the GadX protein"
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Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, (Liang PH), Wang AH (2011-01) Journal of structural biology 173(1), 46-56 "Structural and functional analysis of three beta-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis."
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(Po-Huang Liang) (2011) CHEMISTRY AND PHYSICS OF LIPIDS 164(S1), S12-S14 "Kinetic, mechanistic, structural and inhibitory aspects of the cis-prenyltransferase catalyzed lipid chain elongation."
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Wen, C. C., Shyur, L. F., Jan, J. T., (Po-Huang Liang), Kuo, C. J., Arulselvan, P., Wu, J. B., Kuo, S. C. And Yang, N. S. (2011) Journal of Traditional and Complementary Medicine 1, 41-50 "Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication"
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Ramajayam R, Tan KP, Liu HG, (Liang PH) (2010-11) BIOORGANIC & MEDICINAL CHEMISTRY 18(22), 7849-7854 "Synthesis and evaluation of pyrazolone compounds as SARS-coronavirus 3C-like protease nhibitors"
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Lu YP, Liu HG, Teng KH, (Liang PH) (2010-10) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 400(4), 758-762 "Mechanism of cis-prenyltransferase reaction probed by substrate analogues"
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Ramajayam R, Tan KP, Liu HG, (Liang PH) (2010-06) Bioorganic & medicinal chemistry letters 20(12), 3569-3572 "Synthesis, docking studies, and evaluation of pyrimidines as inhibitors of SARS-CoV 3CL protease"
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Chang TH, Hsieh FL, Ko TP, Teng KH, (Liang PH), Wang AH (2010-02) PLANT CELL 22(2), 454-467 "Structure of a Heterotetrameric Geranyl Pyrophosphate Synthase from Mint (Mentha piperita) Reveals Intersubunit Regulation."
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Chan WC, (Liang PH), Shih YP, Yang UC, Lin WC, Hsu CN.* (2010-01) BMC Bioinformatics 11(Suppl 1), S21 "Learning to predict expression efficacy of vectors in recombinant protein production"
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Wang HM, (Liang PH) (2010-01) Expert opinion on therapeutic patents 20(1), 59-71 "Picornaviral 3C protease inhibitors and the dual 3C protease/coronaviral 3C-like protease inhibitors."
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Ahn TY, Kuo CJ, Liu HG, Ha DC, (Liang PH), Jung YS (2010-01) BULLETIN OF THE KOREAN CHEMICAL SOCIETY 31(01), 87-91 "Synthesis and evaluation of benzoquinolinone derivatives as SARS-CoV 3CL protease inhibitors"
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Kuo CJ, Shih YP, Kan D, Liang PH (2009-12) BioTechniques 47(6), 1029–1032 "Engineering a novel endopeptidase based on SARS 3CLpro"
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Hui-Min Wang, Yan-Ping Shih, Su-Ming Hu, Wen-Tsung Lo, Hui-Min Lin, Shih-Shiu Ding, Hsin-Chi Liao, and (Po-Huang Liang)* (2009-11) Biotechnology Progress 25(6), 1582-1586 "Parallel gene cloning and protein production in multiple expression systems"
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Chia-Hsiang Lo, Ying-Hsuan Chang, Jon D. Wright, Shih-Hsun Chen, Daphne Kan, Carmay Lim, and (Po-Huang Liang)* (2009) Journal of the American Chemical Society 131, 4051-4062 "Combined experimental and theoretical study of long-range interactions modulating dimerization and activity of yeast geranylgeranyl diphosphate synthase"
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Lu YP, Liu HG, Liang PH (2009) Biochemical and biophysical research communications 379(2), 351-355 "Different reaction mechanisms for cis- and trans-prenyltransferases."
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Kuo CJ, Liu HG, Lo YK, Seong CM, Lee KI, Jung YS, Liang PH (2009) FEBS letters 583(3), 549-555 "Individual and common inhibitors of coronavirus and picornavirus main proteases."
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Yuan-Feng Lin, Wen-Ping Tsai, Hon-Ge Liu, and (Po-Huang Liang)* (2009) Cancer Research 69, 6879-6888 "Intracellular beta-tubulin/Chaperonin containing TCP1-beta complex serves as a novel chemotherapeutic target against drug-resistant tumors"
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(Po-Huang Liang)* (2009) Biochemistry 48, 6562-6570 "Reaction kinetics, catalytic mechanisms, conformational changes, and inhibitor design for prenyltransferases"
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Lee CC, Kuo CJ, Ko TP, Hsu MF, Tsui YC, Chang SC, Yang S, Chen SJ, Chen HC, Hsu MC, Shih SR, (Liang PH), Wang AH (2009) The Journal of biological chemistry 284(12), 7646-7655 "Structural basis of inhibition specificities of 3C and 3C-like proteases by zinc-coordinating and peptidomimetic compounds."
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Lee JC, Shih SR, Chang TY, Tseng HY, Shih YF, Yen KJ, Chen WC, Shie JJ, Fang JM, Liang PH, Chao YS, Hsu JT. (2008) Anal Biochemistry 375, 115-123 "A mammalian cell-based reverse two-hybrid system for functional analysis of 3C viral protease of human enterovirus 71"
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C. J. Kuo, J. J. Shie, J. M. Fang, G. R. Yen, J. T. Hsu, H. G. Liu, S. N. Tseng, S. C. Chang, C. Y. Lee, S. R. Shih and P. H. Liang (2008) BIOORGANIC & MEDICINAL CHEMISTRY 16, 7388-7398 "Design, synthesis, and evaluation of 3C protease inhibitors as anti-enterovirus 71 agents"
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Y. M. Shao, W. B. Yang, T. H. Kuo, K. C. Tsai, C. H. Lin, A. S. Yang, P. H. Liang and C. H. Wong (2008) BIOORGANIC & MEDICINAL CHEMISTRY 16, 4652-4660 "Design, Synthesis, and evaluation of trifluoromethyl ketones as inhibitors of SARS-CoV 3CL protease"
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K. M. Chen et al. (2008) J. Med. Chem 51, 5594-5607 "Inhibition of geranylgeranyl diphosphate synthase by bisphosphonates: a crystallographic and computational investigation"
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C. J. Kuo, R. T. Guo, I. L. Lu, H. G. Liu, S. Y. Wu, T. P. Ko, A. H. J. Wang and P. H. Liang* (2008) J Biomed Biotechnol 841312 "Structure-based inhibitors exhibited differential activities against H. pylori and E. coli undecaprenyl pyrophosphate synthases"
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R. T. Guo, R. Cao, P. H. Liang, T. P. Ko, T. H. Chang, M. P. Hudock, W. Y. Jeng, C. K. Chen, Y. Zhang, Y. Song, C. J. Kuo, F. Yin, E. Oldfield and A. H. Wang (2007) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104, 10022-10027 "Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases"
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P. H. Liang (2007) Drugs of the Future 32, 29-29 "Drug discovery for SARS and other human coronaviruses targeting 3C proteases"
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C. W. Yang, Y. N. Yang, P. H. Liang, C. M. Chen, W. L. Chen, H. Y. Chang, Y. S. Chao and S. J. Lee (2007) Antimicrob Agents Chemother 51(11), 3924-3931 "Novel small-molecule inhibitors of transmissible gastroenteritis virus"
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H. M. Wang and P. H. Liang (2007) Expert Opinion on Therapeutic Patents 17, 533-546 "Pharmacophores and biological activities of severe respiratory syndrome viral protease inhibitors"
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C. C. Wen, Y. H. Kuo, J. T. Jan, P. H. Liang, S. Y. Wang, H. G. Liu, C. K. Lee, S. T. Chang, C. J. Kuo, S. S. Lee, C. C. Hou, P. W. Hsiao, S. C. Chien, L. F. Shyur and N. S. Yang (2007) J Med Chem 50, 4087-4095 "Specific Plant Terpenoids and Lignoids Possess Potent Antiviral Activities against Severe Acute Respiratory Syndrome Coronavirus"
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C. C. Lee, C. J. Kuo, M. F. Hsu, P. H. Liang, J. M. Feng, J. J. Shie and A. H. J. Wang (2007) FEBS Lett 581, 5454-5458 "Structural basis of mercury- and zinc-conjugated complexes as SARS-CoV 3C-like protease inhibitors"
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Y. M. Shao, W. B. Yang, H. P. Peng, M. F. Hsu, K. C. Tsai, T. H. Kuo, A. H. Wang, P. H. Liang, C. H. Lin, A. S. Yang and C. H. Wong (2007) Chembiochem 8, 1654-1657 "Structure-Based Design and Synthesis of Highly Potent SARS-CoV 3CL Protease Inhibitors"
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Y. Y. Lee, H. T. Hu, P. H. Liang and C. F. Chak (2006) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 345, 1579-1585 "An E. coli lon mutant conferring partial resistance to colicin may reveal a novel role in regulating proteins involved in the translocation of colicin"
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P. H. Liang (2006) Current Topics in Medicinal Chemistry 6, 361-376 "Characterization and inhibition of SARS-coronavirus main protease"
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D. H. Chang, R. T. Guo, T. P. Ko, A. H.-J. Wang and P. H. Liang (2006) J Biol Chem 281, 14991-15000 "Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination"
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K. C. Tsai, S. Y. Chen, P. H. Liang, I. L. Luo, N. Mahindroo, S. P. Hsieh, Y. S. Chao, W. Lien, T. H. Lin and S. Y. Wu (2006) J Med Chem 49, 3485-3495 "Discovery of a novel family of SARS-CoV protease inhibitors by virtual screening and 3D-QSAR studies"
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T. H. Kuo, S. C. Chuang, S. Y. Chang and P. H. Liang (2006) Biochem J 393, 757-766 "Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping"
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C. Y. Wu, K. Y. King, C. J. Kuo, Y. T. Wu, M. Y. Ho, C. L. Liao, J. M. Fang, P. H. Liang and C. H. Wong (2006) Chem Biol 13, 261-268 "Stable benzotriazole esters as mechanism-based inhibitors of the severe acute respiratory syndrome 3CL protease"
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I. L. Lu, N. Mahindroo, P. H. Liang, C. J. Kuo, H. P. Hsieh, Y. S. Chao and S. Y. Wu (2006) J Med Chem 49, 5154-5161 "Structure-Based Drug Design and Structural Biology Study of Novel Nonpeptide Inhibitors of SARS-CoV Main Protease"
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Y. H. Pan, C. C. Liao, C. C. Kuo, K. J. Duan, P. H. Liang, H. S. Yuan, S. T. Hu and K. F Chak (2006) J Biol Chem 281, 13083-13091 "The critical roles of polyamines in regulating ColE7 production and restricting ColE7 uptake of the colicin producing Escherichia coli"
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R. T. Guo, T. P. Ko, A. P. C. Chen, C. J. Kuo, A. H. J. Wang and P. H. Liang (2005) J Biol Chem 280, 20762-20774 "Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: Roles of the metal ion and conserved residues in catalysis"
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J. J. Shie, J. M., Fang, C. J. Kuo, T. H. Kuo, P. H. Liang, H. J. Huang, W. B. Yang, C. H. Lin, J. L. Chen, Y. T. Wu and C. H. Wong (2005) J Med Chem 48, 4469-4473 "Discovery of potent anilide inhibitors against the severe acute respiratory syndrome 3CL protease"
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Han-Yu Sun, Tzu-Ping Ko, Chih-Jung Kuo, Rey-Ting Guo, Chia-Cheng Chou, Po-Huang Liang and Andrew H.-J. Wang* (2005) J Bacteriol 187(23), 8137-8148 "Homodimeric Hexaprenyl Pyrophosphate Synthase from the Thermoacidophilic Crenarchaeon Sulfolobus solfataricus Displays Asymmetric Subunit Structures"
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Chia-Nan Chen, Coney P. C. Lin, Kuo-Kuei Huang, Wei-Cheng Chen, Hsin-Pang Hsieh, Po-Huang Liang and John T.-A. Hsu* (2005) Evid Based Complement Alternat Med 2(2), 209-215 "Inhibition of SARS-CoV 3C-like Protease Activity by Theaflavin-3,3′-digallate (TF3)"
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J. J. Shie, J. M. Fang, T. H. Kuo, C. J. Kuo, P. H. Liang, H. J. Huang, Y. T. Wu, J. T. Jan, Y. S. Cheng,and C. H. Wong (2005) BIOORGANIC & MEDICINAL CHEMISTRY 13, 5240-5252 "Inhibition of the severe acute respiratory syndrome 3CL protease by peptidomimetic alpha,beta-unsaturated esters."
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M. F. Hsu, C. J. Kuo, K. T. Chang, H. C. Chang, C. C., Chou, T. P. Ko, H. L. Shr, G. G. Chang, A, H.-J. Wang and P. H. Liang (2005) J Biol Chem 280, 31257-31266 "Mechanism of the maturation process of SARS-CoV 3CL protease"
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A. P. Chen, S. Y. Chang, Y. C. Lin, Y. S. Sun, C. T. Chen, A. H. Wang and P. H. Liang (2005) Biochem J 386, 169-176 "Substrate and product specificities of cis-type undecaprenyl pyrophosphate synthase"
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R. T. Guo, C. J. Kuo, T. P. Ko, C. C. Chou, P. H. Liang and A. H. Wang (2004) Biochemistry 43, 7678-7686 "A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products"
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C. J. Kuo, Y. H. Chi, J. T.A. Hsu and P. H. Liang (2004) Biochem Biophys Res Commun 318, 862-867 "Characterization of SARS main protease and inhibitor assay using a fluorogenic substrate"
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R. T. Guo, C. J. Kuo, C. C. Chou, T. P. Ko, H. L. Shr, P. H. Liang and A. H. Wang (2004) J Biol Chem 279, 4903-4912 "Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination"
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K. C. Hsia, K. F. Chak, P. H. Liang, Y. S. Cheng, W. Y. Ku and H. S. Yuan (2004) Structure (Camb) 12, 205-214 "DNA binding and degradation by the HNH protein ColE7"
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J. T. Hsu, C. J. Kuo, H. P. Hsieh, Y. C. Wang, K. K. Huang, C. P. Lin, P. F. Huang, X. Chen and P. H. Liang (2004) FEBS Lett 574, 116-120 "Evaluation of metal-conjugated compounds as inhibitors of 3CL protease of SARS-CoV"
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T. L. Chung, H. H. Hsiao, Y. Y. Yeh, H. L. Shia, Y. L. Chen, P. H. Liang, A. H. Wang, K. H. Khoo and S. L. Li (2004) J Biol Chem 279, 39653-39662 "In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive identification of the modification sites by tandem mass spectrometry analysis of the isopeptides"
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Y. H. Lin, C. C. Liao, P. H. Liang, H. S. Yuan and K. F. Chak (2004) Biochem Biophys Res Commun 318, 81-87 "Involvement of colicin in the limited protection of the colicin producing cells against bacteriophage"
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C. H. Chien, L. H. Huang, C. Y. Chou, Y. S. Chen, Y. S. Han, G. G. Chang, P. H. Liang and X. Chen (2004) J Biol Chem 279, 52338-52345 "One site mutation disrupts dimer formation in human DPP-IV proteins"
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Y. S. Chen, C. H. Chien, C. M. Goparaju, J. T. Hsu, P. H. Liang and X. Chen (2004) Protein Expr Purif 35, 142-146 "Purification and characterization of human prolyl dipeptidase DPP8 in Sf9 insect cells"
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C. Y. Wu, J. T. Jan, S. H. Ma, C. J. Kuo, H. F. Juan, Y. S. Cheng, H. H. Hsu, H. C. Huang, D. Wu, A. Brik, F. S. Liang, R. S. Liu, J. M. Fang, S. T. Chen, P. H. Liang and C. H. Wong (2004) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101, 10012-10017 "Small molecules targeting severe acute respiratory syndrome human coronavirus"
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S. Y. Chang, T. P. Ko, A. P. Chen, A. H. Wang and P. H. Liang (2004) Protein Sci 13, 971-978 "Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies"
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S. Y. Chang, T. P. Ko, P. H. Liang and A. H. Wang (2003) J Biol Chem 278, 29298-29307 "Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and triton"
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S. Y. Chang, Y. K. Chen, A. H. Wang and P. H. Liang (2003) Biochemistry 42, 14452-14459 "Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase"
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R. T. Guo, T. P. Ko, C. C. Chou, H. L. Shr, H. M. Chu, Y. H. Tsai, P. H. Liang and A. H. Wang (2003) ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 59, 2265-2268 "Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli"
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C. E. Atreya, E. F. Johnson, J. Williamson, S. Y. Chang, P. H. Liang and K. S. Anderson (2003) J Biol Chem 278, 28901-28911 "Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis"
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S. M. Hu, P. H. Liang, C. D. Hsiao and C. Wang (2002) Arch Biochem Biophys 407, 135-141 "Characterization of the L399P and R447G mutants of hsc70: the decrease in refolding activity is correlated with an increase in the rate of substrate dissociation"
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J. J. Pan, T. H. Kuo, Y. K. Chen, L. W. Yang and P. H. Liang (2002) BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1594, 64-73 "Insight into the activation mechanism of Escherichia coli octaprenyl pyrophosphate synthase derived from pre-steady-state kinetic analysis"
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Y. H. Chen, A. P. Chen, C. T. Chen, A. H. Wang and P. H. Liang (2002) J Biol Chem 277, 7369-7376 "Probing the conformational change of Escherichia coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants"
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T. H. Kuo, P. H. Liang (2002) Biochimica et Biophysica Acta-Proteins and Proteomics 1599, 125-133 "Reaction kinetic pathway of the recombinant octaprenyl pyrophosphate synthase from Thermotoga maritima: how is it different from that of the mesophilic enzyme"
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P. H. Liang, T. P. Ko and A. H. Wang (2002) Eur J Biochem 269, 3339-3354 "Structure, mechanism and function of prenyltransferases"
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A. P. Chen, Y. H. Chen, H. P. Liu, Y. C. Li, C. T. Chen and P. H. Liang (2002) J Am Chem Soc 124, 15217-15224 "Synthesis and application of a fluorescent substrate analogue to study ligand interactions for undecaprenyl pyrophosphate synthase"
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W. Y. Ku, Y. W. Liu, Y. C. Hsu, C. C. Liao, P. H. Liang, H. S. Yuan and K. F. Chak (2002) Nucleic Acids Res 30, 1670-1678 "The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis"
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T. P. Ko, Y. K. Chen, H. Robinson, P. C. Tsai, Y. G. Gao, A. P. Chen, A. H. Wang and P. H. Liang (2001) J Biol Chem 276, 47474-47482 "Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis"
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C. C. Liao, K. C. Hsiao, Y. W. Liu, P. H. Leng, H. S. Yuen and K. F. Chak (2001) Biochem Biophys Res Commun 284, 556-562 "Processing of DNase domain during translocation of colicin E7 across the membrane of Escherichia coli"
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S. Y. Chang, P. C. Tsai, C. S. Tseng and P. H. Liang (2001) Protein Expr Purif 23, 432-439 "Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli"
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G. Yang, R. B. Kirkpatrick, T. Ho, G. F. Zhang, P. H. Liang, K. O. Johanson, D. J. Casper, M. L. Doyle, J. P. Marino Jr, S. K. Thompson, W. Chen, D. G. Tew and T. D. Meek (2001) Biochemistry 40, 10645-10654 "Steady-state kinetic characterization of substrates and metal-ion specificities of the full-length and N-terminally truncated recombinant human methionine aminopeptidases (type 2)"
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J. J. Pan, L. W. Yang and P. H. Liang (2000) Biochemistry 39, 13856-13861 "Effect of site-directed mutagenesis of the conserved aspartate and glutamate on E. coli undecaprenyl pyrophosphate synthase catalysis"
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D. Lee, S. A. Long, J. L. Adams, G. Chan, K. S. Vaidya, T. A. Francis, K. Kikly, J. D. Winkler, C. M. Sung, C. Debouck, S. Richardson, M. A. Levy, W. E. DeWolf, Jr., P. M. Keller, T. Tomaszek, M. S. Head, M. D. Ryan, R. C. Haltiwanger, P. H. Liang, C. A. Janson, P. J. McDevitt, K. Johanson, N. O. Concha, W. Chan, S. S. Abdel-Meguid, A. M. Badger, M. W. Lark, D. P. Nadeau, L. J. Suva, M. Gowen and M. E. Nuttall (2000) J Biol Chem 275, 16007-16014 "Potent and selective nonpeptide inhibitors of caspases 3 and 7 inhibit apoptosis and maintain cell functionality"
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J. J. Pan, S. T. Chiou and P. H. Liang (2000) Biochemistry 39, 10936-10942 "Product distribution and pre-steady-state kinetic analysis of Escherichia coli undecaprenyl pyrophosphate synthase reaction"
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Jian Dong , Lusong Luo , Po-Huang Liang , Debra Dunaway-Mariano , Paul R. Carey * (2000) JOURNAL OF RAMAN SPECTROSCOPY 31, 365-371 "Raman difference spectroscopic studies of dithiobenzoyl substrate and product analogs binding to the enzyme dehalogenase:ppi-electron polarization is prevented by the C=O to C=S substitution."
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A. Sachpatzidis, C. Dealwis, J. B. Lubetsky, P. H. Liang, K. S. Anderson and E. Lolis (1999) Biochemistry 38, 12665-12674 "Crystallographic Studies of Phosphonat-Based alpha-reaction transition-State Analogues Complexed to Tryptophan Synthase"
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P. H. Liang, J. Lewis, K. S. Anderson, A. Kohen, F. W. D’Souza, Y. Benenson and T. Bassov (1998) Biochemistry 37, 16390-16399 "Catalytic Mechanism of Kdo8P Synthase: Transient Kinetic Studies and Evaluation of a Putative Reaction Intermediate"
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P. H. Liang and K. S. Anderson (1998) Biochemistry 37, 12206-12212 "Kinetic Reaction Scheme for the Dihydrofolate Reductase Domain of the Bifunctional Thymidylate Synthase - Dihydrofolate Reductase from Leishmania Major"
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J. Luba, B. Nare, P. H. Liang, K. S. Anderson, S. M. Beverley and L. W. Hardy (1998) Biochemistry 37, 4093-4104 "Leishmania Major Pteridine Reductase 1 Belongs to the Short Chain Dehydrogenase Family: Stereochemical and Kinetic Evidence"
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T. R. Schneider, E. Gerhardt, M. Lee, P. H. Liang, K. S. Anderson and I. Schlichting (1998) Biochemistry 37, 5394-5406 "Loop Closure and Intersubunit Communication in Tryptophan Synthase"
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P. H. Liang, K. A. Brun, J. A. Field, K. O’Donnell, M. L. Doyle, S. M. Green, A. E. Baker, M. N. Blackburn and S. S. Abdel-Meguid (1998) Biochemistry 37, 5923-5929 "Site-Directed Mutagenesis Probing the Catalytic Role of Arginines 165 and 166 of Human Cytomegalovirus Protease"
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P. H. Liang and K. S. Anderson (1998) Biochemistry 37, 12195-12205 "Substrate Channeling and Domain-Domain Interactions in Bifunctional Thymidylate Synthase - Dihydrofolate Reductase"
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P. H. Liang, A. Kohen, T. Baasov T and K. S. Anderson (1997) Bioorg Med Chem Lett 7, 2463-2468 "Catalytic Mechanism of KDO8P Synthase, Pre-Steady-State Kinetic Analysis Using Rapid Chemical Quench Flow Methods"
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D. E. Cane, H. T. Chiu, P. H. Liang and K. S. Anderson (1997) Biochemistry 36, 8332-8339 "Pre-Steady-State Kinetic Analysis of the Trichodiene Synthase Reaction Pathway"
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K. L. Taylor, R. Q. Liu, P. H. Liang, J. Price, D. Dunaway-Mariano, P. J. Tonge, J. Clarkson and P. R. Carey (1995) Biochemistry 34, 13881-13888 "Evidence for Electrophilic Catalysis in the 4-Chlorobenzoyl-CoA Dehalogenase Reaction: UV, Raman and C-13 NMR Spectral Studies of Dehalogenase Complexes of Benzoyl-CoA Adducts"
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R. Q. Liu, P. H. Liang, J. D. Scholten and D. Dunaway-Mariano (1995) J Am Chem Soc 117, 5003-5004 "Transient State Kinetic Analysis of the Chemical Intermediate Formed in the Enzymatic Dehalogenation of 4-Chlorobenzoyl Coenzyme A"
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G. Yang, P.H. Liang and D. Dunaway-Mariano (1994) Biochemistry 33, 8527-8531 "Evidence for Nucleophilic Catalysis in the Aromatic Substitution Reaction Catalyzed by (4-Chlorobenzoyl) coenzyme A dehalogenase"
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P. H. Liang, G. Yang and D. Dunaway-Mariano (1993) Biochemistry 32, 12245-12250 "Specificity of 4-chlorobenzoate-Coenzyme A dehalogenase catalyzed dehalogenation of halogenated aromatics"
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P. C. Babbitt, G. L. Kenyon, B. M. Martin, H. Charest, M. Sylvester, J. D. Scholten, K. H. Chang, P. H. Liang and D. Dunaway-Mariano (1992) Biochemistry 31, 5594-5604 "Ancestry of the 4-chlorobenzoate dehalogenase: Analysis of amino acid sequence identities among families of acyl: adenyl ligase, enoyl-CoA hydratase/isomerase, and acyl-CoA thioesterase"
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K. H. Chang, P. H. Liang, W. Beck, J. D. Scholten and D. Dunaway-Mariano (1992) Biochemistry 31, 5605-5610 "Isolation and characterization of the three polypeptide components of 4-chlorobenzoate dehalogenase from Pseudomonas sp. Strain CBS-3"