Research

Our research interests are mainly focused on the structure, function, and biophysical properties of proteins. The information we discovered can further guide the inhibitor design to aid in cancer therapy or enzyme engineering for industrial uses. We conduct our studies primarily using X-ray crystallography, spectroscopy and enzymology and routinely collaborates with other laboratories worldwide. Your can find more about us at http://ho.ibc.sinica.edu.tw/

Our current studies involves three areas: (1) reconstruct the rice survival strategy under submergence (2) protein-protein interaction related to cancer (3) enzyme engineering for industrial use.

  1. Reconstruct the rice survival strategy under submergence:
    • Most rice cultivars die within a week of complete submergence, but some rice cultivars show high tolerance and survive up to two weeks under complete submergence. It’s believed that Sub1A is the key transcription factor that regulates quiescence responses enabling prolonged submergence. We aim to understand Sub1A1 regulatory networks. We will study the DNA binding specificity of Sub1A-1 to reveal the downstream signaling pathway via biochemical and biophysical approaches combined with our microarray data and the functions of other domains by structural approaches.
  2. Protein-protein interaction related to cancer:
    • The receptor-like protein tyrosine phosphatase-α (rPTPα) is a crucial player in mediating RTK/Src signal transduction pathways. Because rPTPα can activate Src and is overexpressed in various cancers, rPTPα is considered as proto-oncogene. The rPTPα can be phosphorylated by RTK to become the active form, and is also subject to oxidation by ROS. However, detailed information at the molecular level is missing largely due to lack of structures of true protein-phosphoprotein complexes. The major challenges in such structural studies include 1) to produce a large quantity of protein-phosphoprotein complexes involving specifically modified rPTPα; 2) to crystallize the protein complexes. We would like to address a) how rPTPα activates the activity of Src b) how oxidation of rPTPα affects its activity to activate Src.
    • Protein arginine methyltransferases (PRMTs) play roles in cancer progression by methylating many cancer related proteins. However, the question remains which protein methylation leads to oncogenic activation. Our structural approaches focusing on PRMT-protein complex provide a way to answer this question and can assist inhibitor design to prevent methylation for cancer therapy.
    • KLHL20, a protein adaptor, interacts with the tumor suppressor, PML. This interaction induces the degradation of PML which leads to tumor progression. Inhibitors that prevent KLHL20-PML interaction may be useful in cancer therapy. We are now working on the KLHL20-PML complex structure which can provide a blueprint for inhibitor design.
  3. Enzyme engineering for industrial use:
    • Many enzymes that catalyze specific reactions have industrial applications. The biochemical/biophysical modifications of enzymes by protein engineering can broaden the uses in the industry. Based on structural analyses, we aim to improve chemical properties of cellulase (substrate variety) for biofuel application and physical properties (heat and pH tolerance) of our patented keratinase for animal feed application.
Degrees and Positions Held
Positions Held
  • 2019 – present   Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2011 – 2019   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2007 – 2011   postdoctoral researcher, Albert Einstein College of Medicine of Yeshiva University
Degrees
  • 2001 – 2007   Ph.D., Physiology & Biophysics, Boston University
  • 1995 – 1999   B.S., Chemistry, National Tsing-Hua University
Selected Publications
Yan L, Yan C, Qian K, Su H, Kofsky-Wofford SA, Lee WC, Zhao X, Ho MC, Ivanov I, Zheng YG
Journal of medicinal chemistry (2014)
Suarez J, Haapalainen AM, Cahill SM, Ho MC, Yan F, Almo SC, Schramm VL
Chemistry & biology (2013)
Meng-Chiao Ho, Carola Wilczek, Jeffrey B. Bonanno, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G. Carter, Takashi Onikubo, P. Rajesh Kumar, Man K. Chan, Michael Brenowitz, R. Holland Cheng, Ulf Reimer, Steven C. Almo, David Shechter
PLoS ONE (2013)
Hemeon I, Gutierrez J.A., Ho MC., Schramm V.L.
Anal Chem. (2011)
Guan R, Ho MC, Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL
Biochemistry (2011)
Guan R, Ho MC, Almo SC, Schramm VL
Biochemistry (2011)
Ho MC, Shi W, Rinaldo-Matthis A, Tyler PC, Evans GB, Clinch K, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2010)
Burgos ES, Ho MC, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2009)
Ho MC, Menetret JF, Tsuruta H, Allen KN
Nature (2009)
Ho MC, Sturm MB, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2009)
Publications List
  1. Anandharaj M, Lin YJ, Rani RP, Nadendla EK, (Ho MC), Huang CC, Cheng JF, Chang JJ, Li WH  (2020-02)  Proceedings of the National Academy of Sciences of the United States of America  117(5), 2385-2394  "Constructing a yeast to express the largest cellulosome complex on the cell surface."
  2. Tewary SK, Zheng YG, (Ho MC*)  (2019-08)  Cellular and Molecular Life Sciences  76(15), 2917-2932  "Protein arginine methyltransferases: insights into the enzyme structure and mechanism at the atomic level."
  3. Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, Wu WJ, Wu JL, Gibbs DJ, (Ho MC*), Shih MC  (2019-02)  Proceedings of the National Academy of Sciences of the United States of America  116(8), 3300-3309  "Regulatory cascade involving transcriptional and N-end rule pathways in rice under submergence."
  4. Liang PH, Lin WL, Hsieh HY, Lin TY, Chen CH, Tewary SK, Lee HL, Yuan SF, Yang B, Yao JY, (Ho MC)  (2018-03)  Biochimica et Biophysica Acta - General Subjects  1862(3), 513-521  "A flexible loop for mannan recognition and activity enhancement in a bifunctional glycoside hydrolase family 5."
  5. Fulton MD, Zhang J, He M, (Ho MC), Zheng YG  (2017-07)  Biochemistry  56(28), 3539-3548  "Intricate Effects of alpha-Amino and Lysine Modifications on Arginine Methylation of the N-Terminal Tail of Histone H4."
  6. Wu WL, Chen MY, Tu IF, Lin YC, EswarKumar N, Chen MY, (Ho MC*), Wu SH  (2017-07)  Scientific reports  7(1), 4658  "The discovery of novel heat-stable keratinases from Meiothermus taiwanensis WR-220 and other extremophiles."
  7. Hu H, Qian K, (Ho MC), Zheng YG  (2016-03)  Expert opinion on investigational drugs  25(3), 335-358  "Small Molecule Inhibitors of Protein Arginine Methyltransferases."
  8. Liu MS, Tsai HY, Liu XX, (Ho MC), Wu WJ*, Tsai MD*  (2016-02)  Journal of the American Chemical Society  138(7), 2389-2398  "Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ"
  9. Lee WC, Lin WL, Matsui T, Chen ES, Wei TW, Lin WH, Hu H, Zheng YG, Tsai MD, (Ho MC)  (2015-12)  Biochemistry  54(51), 7514-7523  "PROTEIN ARGININE METHYLTRANSFERASE 8: tetrameric structure and protein substrate specificity."
  10. Yuan SF, Wu TH, Lee HL, Hsieh HY, Lin WL, Yang B, Chang CK, Li Q, Gao J, Huang CH, (Ho MC)*, Guo RT, Liang PH  (2015-02)  The Journal of biological chemistry  290(9), 5739-5748  "Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum."
  11. Yan L, Yan C, Qian K, Su H, Kofsky-Wofford SA, Lee WC, Zhao X, (Ho MC), Ivanov I, Zheng YG  (2014)  Journal of medicinal chemistry  57(6), 2611-2622  "Diamidine compounds for selective inhibition of protein arginine methyltransferase 1."
  12. Donaldson TM, Cassera MB, (Ho MC), Zhan C, Merino EF, Evans GB, Tyler PC, Almo SC, Schramm VL, Kim K  (2014)  Eukaryotic cell  13(5), 572-579  "INHIBITION AND STRUCTURE OF TOXOPLASMA GONDII PURINE NUCLEOSIDE PHOSPHORYLASE."
  13. (Meng-Chiao Ho)*, Carola Wilczek, Jeffrey B. Bonanno, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G. Carter, Takashi Onikubo, P. Rajesh Kumar, Man K. Chan, Michael Brenowitz, R. Holland Cheng, Ulf Reimer, Steven C. Almo, David Shechter*  (2013-02)  PLoS ONE  8(2), e57008  "Structure of the Arginine Methyltransferase PRMT5-MEP50 Reveals a Mechanism for Substrate Specificity."
  14. Suarez J, Haapalainen AM, Cahill SM, (Ho MC), Yan F, Almo SC, Schramm VL  (2013)  Chemistry & biology  20(2), 212-222  "Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography."
  15. Guan R, (Ho MC), Frohlich RF, Tyler PC, Almo SC, Schramm VL*  (2012-11)  Biochemistry  51(45), 9094-9103  "Methylthioadenosine Deaminase in an Alternative Quorum Sensing Pathway in Pseudomonas aeruginosa."
  16. Ke S, (Ho MC), Zhadin N, Deng H, Callender R.*  (2012-05)  J Phys Chem B  116(21), 6166-6176  "Investigation of catalytic loop structure, dynamics, and function relationship of Yersinia protein tyrosine phosphatase by temperature-jump relaxation spectroscopy and X-ray structural determination."
  17. Cassera MB, (Ho MC), Merino EF, Burgos ES, Rinaldo-Matthis A, Almo SC, Schramm VL  (2011)  Biochemistry  50(11), 1885-1893  "A high-affinity adenosine kinase from Anopheles gambiae."
  18. Hemeon I, Gutierrez J.A., (Ho MC.), Schramm V.L.  (2011)  Anal Chem.  83(12), 4996-5004  "Characterizing DNA methyltransferases with an ultrasensitive luciferase-linked continuous assay."
  19. Guan R, (Ho MC), Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL  (2011)  Biochemistry  50(47), 10408-10417  "Entropy-driven binding of picomolar transition state analogue inhibitors to human 5'-methylthioadenosine phosphorylase."
  20. Guan R, (Ho MC), Almo SC, Schramm VL  (2011)  Biochemistry  50(7), 1247-1254  "Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing."
  21. Cassera MB, Hazleton KZ, Merino EF, Obaldia N 3rd, (Ho MC), Murkin AS, Depinto R, Gutierrez JA, Almo SC, Evans GB, Babu YS, Schramm VL  (2011)  PloS one  6(11), e26916  "Plasmodium falciparum Parasites Are Killed by a Transition State Analogue of Purine Nucleoside Phosphorylase in a Primate Animal Model."
  22. (Ho MC), Shi W, Rinaldo-Matthis A, Tyler PC, Evans GB, Clinch K, Almo SC, Schramm VL  (2010)  Proceedings of the National Academy of Sciences of the United States of America  107(11), 4805-4812  "Four generations of transition-state analogues for human purine nucleoside phosphorylase."
  23. Burgos ES, (Ho MC), Almo SC, Schramm VL  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(33), 13748-13753  "A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT."
  24. (Ho MC), Cassera MB, Madrid DC, Ting LM, Tyler PC, Kim K, Almo SC, Schramm VL  (2009)  Biochemistry  48(40), 9618-9626  "Structural and metabolic specificity of methylthiocoformycin for malarial adenosine deaminases."
  25. (Ho MC), Menetret JF, Tsuruta H, Allen KN  (2009)  Nature  459(7245), 393-397  "The origin of the electrostatic perturbation in acetoacetate decarboxylase."
  26. Gutierrez JA, Crowder T, Rinaldo-Matthis A, (Ho MC), Almo SC, Schramm VL  (2009)  Nature chemical biology  5(4), 251-257  "Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing."
  27. (Ho MC), Sturm MB, Almo SC, Schramm VL  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(48), 20276-20281  "Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins."
  28. Chi Y, Kumar TK, Wang HM, (Ho MC), Chiu IM, Yu C  (2001)  Biochemistry  40(25), 7746-7753  "Thermodynamic characterization of the human acidic fibroblast growth factor: evidence for cold denaturation."

Our research interests are mainly focused on the structure, function, and biophysical properties of proteins. The information we discovered can further guide the inhibitor design to aid in cancer therapy or enzyme engineering for industrial uses. We conduct our studies primarily using X-ray crystallography, spectroscopy and enzymology and routinely collaborates with other laboratories worldwide. Your can find more about us at http://ho.ibc.sinica.edu.tw/

Our current studies involves three areas: (1) reconstruct the rice survival strategy under submergence (2) protein-protein interaction related to cancer (3) enzyme engineering for industrial use.

  1. Reconstruct the rice survival strategy under submergence:
    • Most rice cultivars die within a week of complete submergence, but some rice cultivars show high tolerance and survive up to two weeks under complete submergence. It’s believed that Sub1A is the key transcription factor that regulates quiescence responses enabling prolonged submergence. We aim to understand Sub1A1 regulatory networks. We will study the DNA binding specificity of Sub1A-1 to reveal the downstream signaling pathway via biochemical and biophysical approaches combined with our microarray data and the functions of other domains by structural approaches.
  2. Protein-protein interaction related to cancer:
    • The receptor-like protein tyrosine phosphatase-α (rPTPα) is a crucial player in mediating RTK/Src signal transduction pathways. Because rPTPα can activate Src and is overexpressed in various cancers, rPTPα is considered as proto-oncogene. The rPTPα can be phosphorylated by RTK to become the active form, and is also subject to oxidation by ROS. However, detailed information at the molecular level is missing largely due to lack of structures of true protein-phosphoprotein complexes. The major challenges in such structural studies include 1) to produce a large quantity of protein-phosphoprotein complexes involving specifically modified rPTPα; 2) to crystallize the protein complexes. We would like to address a) how rPTPα activates the activity of Src b) how oxidation of rPTPα affects its activity to activate Src.
    • Protein arginine methyltransferases (PRMTs) play roles in cancer progression by methylating many cancer related proteins. However, the question remains which protein methylation leads to oncogenic activation. Our structural approaches focusing on PRMT-protein complex provide a way to answer this question and can assist inhibitor design to prevent methylation for cancer therapy.
    • KLHL20, a protein adaptor, interacts with the tumor suppressor, PML. This interaction induces the degradation of PML which leads to tumor progression. Inhibitors that prevent KLHL20-PML interaction may be useful in cancer therapy. We are now working on the KLHL20-PML complex structure which can provide a blueprint for inhibitor design.
  3. Enzyme engineering for industrial use:
    • Many enzymes that catalyze specific reactions have industrial applications. The biochemical/biophysical modifications of enzymes by protein engineering can broaden the uses in the industry. Based on structural analyses, we aim to improve chemical properties of cellulase (substrate variety) for biofuel application and physical properties (heat and pH tolerance) of our patented keratinase for animal feed application.
Positions Held
  • 2019 – present   Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2011 – 2019   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2007 – 2011   postdoctoral researcher, Albert Einstein College of Medicine of Yeshiva University
Degrees
  • 2001 – 2007   Ph.D., Physiology & Biophysics, Boston University
  • 1995 – 1999   B.S., Chemistry, National Tsing-Hua University
Yan L, Yan C, Qian K, Su H, Kofsky-Wofford SA, Lee WC, Zhao X, Ho MC, Ivanov I, Zheng YG
Journal of medicinal chemistry (2014)
Suarez J, Haapalainen AM, Cahill SM, Ho MC, Yan F, Almo SC, Schramm VL
Chemistry & biology (2013)
Meng-Chiao Ho, Carola Wilczek, Jeffrey B. Bonanno, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G. Carter, Takashi Onikubo, P. Rajesh Kumar, Man K. Chan, Michael Brenowitz, R. Holland Cheng, Ulf Reimer, Steven C. Almo, David Shechter
PLoS ONE (2013)
Hemeon I, Gutierrez J.A., Ho MC., Schramm V.L.
Anal Chem. (2011)
Guan R, Ho MC, Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL
Biochemistry (2011)
Guan R, Ho MC, Almo SC, Schramm VL
Biochemistry (2011)
Ho MC, Shi W, Rinaldo-Matthis A, Tyler PC, Evans GB, Clinch K, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2010)
Burgos ES, Ho MC, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2009)
Ho MC, Menetret JF, Tsuruta H, Allen KN
Nature (2009)
Ho MC, Sturm MB, Almo SC, Schramm VL
Proceedings of the National Academy of Sciences of the United States of America (2009)
  1. Anandharaj M, Lin YJ, Rani RP, Nadendla EK, (Ho MC), Huang CC, Cheng JF, Chang JJ, Li WH  (2020-02)  Proceedings of the National Academy of Sciences of the United States of America  117(5), 2385-2394  "Constructing a yeast to express the largest cellulosome complex on the cell surface."
  2. Tewary SK, Zheng YG, (Ho MC*)  (2019-08)  Cellular and Molecular Life Sciences  76(15), 2917-2932  "Protein arginine methyltransferases: insights into the enzyme structure and mechanism at the atomic level."
  3. Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, Wu WJ, Wu JL, Gibbs DJ, (Ho MC*), Shih MC  (2019-02)  Proceedings of the National Academy of Sciences of the United States of America  116(8), 3300-3309  "Regulatory cascade involving transcriptional and N-end rule pathways in rice under submergence."
  4. Liang PH, Lin WL, Hsieh HY, Lin TY, Chen CH, Tewary SK, Lee HL, Yuan SF, Yang B, Yao JY, (Ho MC)  (2018-03)  Biochimica et Biophysica Acta - General Subjects  1862(3), 513-521  "A flexible loop for mannan recognition and activity enhancement in a bifunctional glycoside hydrolase family 5."
  5. Fulton MD, Zhang J, He M, (Ho MC), Zheng YG  (2017-07)  Biochemistry  56(28), 3539-3548  "Intricate Effects of alpha-Amino and Lysine Modifications on Arginine Methylation of the N-Terminal Tail of Histone H4."
  6. Wu WL, Chen MY, Tu IF, Lin YC, EswarKumar N, Chen MY, (Ho MC*), Wu SH  (2017-07)  Scientific reports  7(1), 4658  "The discovery of novel heat-stable keratinases from Meiothermus taiwanensis WR-220 and other extremophiles."
  7. Hu H, Qian K, (Ho MC), Zheng YG  (2016-03)  Expert opinion on investigational drugs  25(3), 335-358  "Small Molecule Inhibitors of Protein Arginine Methyltransferases."
  8. Liu MS, Tsai HY, Liu XX, (Ho MC), Wu WJ*, Tsai MD*  (2016-02)  Journal of the American Chemical Society  138(7), 2389-2398  "Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ"
  9. Lee WC, Lin WL, Matsui T, Chen ES, Wei TW, Lin WH, Hu H, Zheng YG, Tsai MD, (Ho MC)  (2015-12)  Biochemistry  54(51), 7514-7523  "PROTEIN ARGININE METHYLTRANSFERASE 8: tetrameric structure and protein substrate specificity."
  10. Yuan SF, Wu TH, Lee HL, Hsieh HY, Lin WL, Yang B, Chang CK, Li Q, Gao J, Huang CH, (Ho MC)*, Guo RT, Liang PH  (2015-02)  The Journal of biological chemistry  290(9), 5739-5748  "Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum."
  11. Yan L, Yan C, Qian K, Su H, Kofsky-Wofford SA, Lee WC, Zhao X, (Ho MC), Ivanov I, Zheng YG  (2014)  Journal of medicinal chemistry  57(6), 2611-2622  "Diamidine compounds for selective inhibition of protein arginine methyltransferase 1."
  12. Donaldson TM, Cassera MB, (Ho MC), Zhan C, Merino EF, Evans GB, Tyler PC, Almo SC, Schramm VL, Kim K  (2014)  Eukaryotic cell  13(5), 572-579  "INHIBITION AND STRUCTURE OF TOXOPLASMA GONDII PURINE NUCLEOSIDE PHOSPHORYLASE."
  13. (Meng-Chiao Ho)*, Carola Wilczek, Jeffrey B. Bonanno, Li Xing, Janina Seznec, Tsutomu Matsui, Lester G. Carter, Takashi Onikubo, P. Rajesh Kumar, Man K. Chan, Michael Brenowitz, R. Holland Cheng, Ulf Reimer, Steven C. Almo, David Shechter*  (2013-02)  PLoS ONE  8(2), e57008  "Structure of the Arginine Methyltransferase PRMT5-MEP50 Reveals a Mechanism for Substrate Specificity."
  14. Suarez J, Haapalainen AM, Cahill SM, (Ho MC), Yan F, Almo SC, Schramm VL  (2013)  Chemistry & biology  20(2), 212-222  "Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography."
  15. Guan R, (Ho MC), Frohlich RF, Tyler PC, Almo SC, Schramm VL*  (2012-11)  Biochemistry  51(45), 9094-9103  "Methylthioadenosine Deaminase in an Alternative Quorum Sensing Pathway in Pseudomonas aeruginosa."
  16. Ke S, (Ho MC), Zhadin N, Deng H, Callender R.*  (2012-05)  J Phys Chem B  116(21), 6166-6176  "Investigation of catalytic loop structure, dynamics, and function relationship of Yersinia protein tyrosine phosphatase by temperature-jump relaxation spectroscopy and X-ray structural determination."
  17. Cassera MB, (Ho MC), Merino EF, Burgos ES, Rinaldo-Matthis A, Almo SC, Schramm VL  (2011)  Biochemistry  50(11), 1885-1893  "A high-affinity adenosine kinase from Anopheles gambiae."
  18. Hemeon I, Gutierrez J.A., (Ho MC.), Schramm V.L.  (2011)  Anal Chem.  83(12), 4996-5004  "Characterizing DNA methyltransferases with an ultrasensitive luciferase-linked continuous assay."
  19. Guan R, (Ho MC), Brenowitz M, Tyler PC, Evans GB, Almo SC, Schramm VL  (2011)  Biochemistry  50(47), 10408-10417  "Entropy-driven binding of picomolar transition state analogue inhibitors to human 5'-methylthioadenosine phosphorylase."
  20. Guan R, (Ho MC), Almo SC, Schramm VL  (2011)  Biochemistry  50(7), 1247-1254  "Methylthioinosine phosphorylase from Pseudomonas aeruginosa. Structure and annotation of a novel enzyme in quorum sensing."
  21. Cassera MB, Hazleton KZ, Merino EF, Obaldia N 3rd, (Ho MC), Murkin AS, Depinto R, Gutierrez JA, Almo SC, Evans GB, Babu YS, Schramm VL  (2011)  PloS one  6(11), e26916  "Plasmodium falciparum Parasites Are Killed by a Transition State Analogue of Purine Nucleoside Phosphorylase in a Primate Animal Model."
  22. (Ho MC), Shi W, Rinaldo-Matthis A, Tyler PC, Evans GB, Clinch K, Almo SC, Schramm VL  (2010)  Proceedings of the National Academy of Sciences of the United States of America  107(11), 4805-4812  "Four generations of transition-state analogues for human purine nucleoside phosphorylase."
  23. Burgos ES, (Ho MC), Almo SC, Schramm VL  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(33), 13748-13753  "A phosphoenzyme mimic, overlapping catalytic sites and reaction coordinate motion for human NAMPT."
  24. (Ho MC), Cassera MB, Madrid DC, Ting LM, Tyler PC, Kim K, Almo SC, Schramm VL  (2009)  Biochemistry  48(40), 9618-9626  "Structural and metabolic specificity of methylthiocoformycin for malarial adenosine deaminases."
  25. (Ho MC), Menetret JF, Tsuruta H, Allen KN  (2009)  Nature  459(7245), 393-397  "The origin of the electrostatic perturbation in acetoacetate decarboxylase."
  26. Gutierrez JA, Crowder T, Rinaldo-Matthis A, (Ho MC), Almo SC, Schramm VL  (2009)  Nature chemical biology  5(4), 251-257  "Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing."
  27. (Ho MC), Sturm MB, Almo SC, Schramm VL  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(48), 20276-20281  "Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins."
  28. Chi Y, Kumar TK, Wang HM, (Ho MC), Chiu IM, Yu C  (2001)  Biochemistry  40(25), 7746-7753  "Thermodynamic characterization of the human acidic fibroblast growth factor: evidence for cold denaturation."