Research

The research goal of my laboratory aims at unraveling the molecular mechanism underlying the differentiation of placental trophoblast cell, which is the first differentiated cell lineage during embryogenesis and is essential for implantation and placentation. Specifically, we study how trophoblast cells undergo cell fusion to form a multinucleated cell layer, the syncytiotrophoblast layer, which mediates gas and nutrient exchange between fetus and mother and produces growth factors and hormones essential for pregnancy maintenance. Recently, we have demonstrated that the placental transcription factor GCMa mediates trophoblastic fusion by upregulating expression of the placental fusogenic protein syncytin and that a decreased expression of GCMa gene is associated with the common pregnancy disorder pre-eclampsia. We have further demonstrated that GCMa activity is controlled by different post-translational modifications. The F-box protein FBW2 recognizes and promotes GCMa ubiquitination for proteasome degradation. On the contrary, the cAMP/PKA signaling pathway stabilizes GCMa protein via its downstream factor CBP, which catalyze GCMa acetylation and protects GCMa from ubiquitination. In addition, GCMa undergoes sumoylation, which decreases the DNA-binding activity of GCMa. Overall, syncytiotrophoblast differentiation can be regulated by post-translational modification of GCMa. The undergoing projects are to study additional signaling pathways and protein modifications that regulate placental cell differentiation.

Degrees and Positions Held
Positions Held
  • 2008 – present   Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2003 – 2008   Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 1996 – 2003   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica.
  • 1994 – 1996   Visiting Fellow , Human Genetics Branch, NICHD, NIH.
  • 1991 – 1994   Adjunct Scientist, Human Genetics Branch, NICHD, NIH.
Degrees
  • 1994   Ph.D., Physiology, University of Florida.
  • 1986   B.S., Zoology, National Taiwan University.
Selected Publications
A novel cAMP/Epac1/CaMKI signaling cascade promotes GCM1 desumoylation and placental cell fusion.
Chang, C.W.; Chang, G.D.; Chen, H.
Mol. Cell. Biol. (2011)
Dual-specificity phosphatase 23 mediates GCM1 dephosphorylation and activation.
Lin, F.Y.; Chang, C.W.; Cheong, M.L.; Chen, H.C.; Lee, D.Y.; Chang, G.D.; Chen, H.
Nucleic Acids Res. (2011)
GCM1 Regulation of the Expression of Syncytin-2 and Its Cognate Receptor MFSD2A in Placenta.
Liang, C.Y.; Wang, L.J.; Chen, C.P.; Chen, L.F.; Chen, Y.H.; Chen, H.
Biol. Reprod. (2010)
Gcm Protein Degradation Suppresses Proliferation of Glial Progenitors.
Ho, M.; Chen, H.; Chen, M.; Jacques, C.; Giangrande, A.; and Chien, C.T.
Proc. Natl. Acad. Sci. USA (2009)
Mechanism of Hypoxia-Induced GCM1 Degradation: Implications for the Pathogenesis of Preeclampsia.
J. Biol. Chem. (2009)
Functional Characterization of the Human Placental Fusogenic Membrane Protein Syncytin 2.
Chen, C.P.; Chen, L.F.; Yang, S.R.; Chen, C.Y.; Ko, C.C.; Chang, G.D.; Chen, H.
Biol. Reprod. (2008)
Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for F-Box and WD Repeat Domain Containing 2-Mediated Human Glial Cell Missing Homolog 1 Ubiquitination and Degradation.
Chiang, M.H.; Chen, L.F.; Chen, H.
Biol. Reprod. (2008)
Small Ubiquitin-like Modifier Modification Regulates the DNA Binding Activity of Glial Cell Missing Drosophila Homolog a.
Chou, C.C.; Chang, C.W.; Liu J.H.; Hsiao, C.D.; Chen, H.
J. Biol. Chem. (2007)
Thiazolidinediones modulate the expression of beta-catenin and other cell-cycle regulatoryproteins by targeting the F-box proteins of Skp1-Cul1-F-box protein E3 ubiquitin ligase independently of peroxisome proliferator-activated receptor gamma.
Wei, S.; Lin, L.F.; Yang, C.C.; Wang, Y.C.; Chang, G.D.; Chen, H.; Chen, C.S.
Mol. Pharm. (2007)
Histone Deacetylase 3 Binds to and Regulates the GCMa Transcription Factor.
Chuang, H.C.; Chang, C.W.; Chang, G.D.; Yao, T.P.; Chen, H.
Nucleic Acids Res. (2006)
Publications List
  1. Wang LJ, Lo HF, Lin CF, Ng PS, Wu YH, Lee YS, Cheong ML, and (Chen H)*  (2019-01)  FASEB JOURNAL  33(1), 314-326  "SFRP3 Negatively Regulates Placental Extravillous Trophoblast Cell Migration Mediated by the GCM1-WNT10B-FZD7 Axis."
  2. Chiu YH, Yang MR, Wang LJ, Chen MH, Chang GD, (Chen H)*  (2018-06)  JOURNAL OF BIOLOGICAL CHEMISTRY  293(25), 9801-9811  "New Insights into the Regulation of Placental Growth Factor Gene Expression by the Transcription Factors GCM1 and DLX3 in Human Placenta."
  3. Lo, H.-F., Tsai, C.-Y., Chen, C.-P., Wang, L.-J., Lee, Y.-S., Chen, C.-Y., Liang, C.-T., Cheong, M.-L., (Chen, H.*)  (2017-02)  The Journal of pathology  241, 324-336  "Association of Dysfunctional Synapse Defective 1 (SYDE1) with Restricted Fetal Growth – SYDE1 Regulates Placental Cell Migration and Invasion"
  4. Chiu, Y. H. and (Chen, H.)  (2016-02)  Scientific Reports  6, 21630  "GATA3 Inhibits GCM1 Activity and Trophoblast Cell Invasion"
  5. Cheong ML, Wang LJ, Chuang PY, Chang CW, Lee YS, Lo HF, Tsai MS, (Chen H)  (2016-01)  Mol Cell Biol  36, 197-209  "A Positive Feedback Loop between Glial Cells Missing 1 (GCM1) and Human Chorionic Gonadotropin (hCG) Regulates Placental hCGbeta Expression and Cell Differentiation"
  6. Chen, Y.-Y., Chuang, P.-Y., Chen, C.-P., Chiu, Y.-H., Lo, H.-F., Cheong, M.-L., Huang, J.-Y., Kuo, P.-L., and (Chen, H.*)  (2014)  J Biol Chem  289(33), 22958-22968  "Functional Antagonism between High-temperature Requirement Protein A (HtrA) Family Members Regulates Trophoblast Invasion"
  7. Chang, C.-W., Cheong, M.-L., Chang, G.-D., Tsai, M.-S., and (Chen, H.*)  (2013-11)  Mol Hum Reprod  19(11), 745-755  "Involvement of Epac1/Rap1/CaMKI/HDAC5 Signaling Cascade in the Regulation of Placental Cell Fusion"
  8. Wu, Y.-H., Lo, H.-F., Chen, S.-H., and (Chen, H.)*  (2013-07)  FASEB J  27(7), 2818-2828  "Caspase-14 Suppresses GCM1 Acetylation and Inhibits Placental Cell Differentiation"
  9. Wang, C.-C., Lo, H.-F., Lin, S.-Y., and (Chen, H.*)  (2013)  Biochem J  453, 201-208  "RACK1 (Receptor for Activated C-kinase 1) Interacts with FBW2 (F-box and WD-repeat domain-containing 2) to Up-regulate GCM1 (Glial Cell Missing 1) Stability and Placental Cell Migration and Invasion"
  10. Wang, L.-J., Cheong, M.-L., Lee, Y.-S., Lee, M.-T., and (Chen, H.*)  (2012-09)  Mol Cell Biol  32(18), 3707-3717  "High-temperature Requirement A4 (HtrA4) Suppresses the Fusogenic Activity of Syncytin-1 and Promotes Trophoblast Invasion"
  11. Chang, C.-W., Chang, G.-D, and (Chen, H*)   (2011-09)  Mol Cell Biol  31(18), 3820-3831  "A Novel cAMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion"
  12. Lin, F.-Y., Chang, C.-W., Cheong, M.-L., Chen, H.-C., Lee, D.-Y., Chang, G.-D., and (Chen, H*)  (2011-02)  Nucleic Acids Res  39(3), 848-861  "Dual-specificity Phosphatase 23 Mediates GCM1 Dephosphorylation and Activation"
  13. Liang, C.-Y., Wang, L.-J., Chen, C.-P., Chen, L.-F., Chen, Y.-H., and (Chen, H*)  (2010)  Biol Reprod  83(3), 387-395  "GCM1 Regulation of the Expression of Syncytin 2 and Its Cognate Receptor MFSD2A in Human Placenta"
  14. Ho MS, (Chen H), Chen M, Jacques C, Giangrande A, Chien CT  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(16), 6778-6783  "Gcm Protein Degradation Suppresses Proliferation of Glial Progenitors"
  15. Chiang MH, Liang FY, Chen CP, Chang CW, Cheong ML, Wang LJ, Liang CY, Lin FY, Chou CC, (Chen H)  (2009)  J Biol Chem  284(26), 17411-17419  "Mechanism of Hypoxia-Induced GCM1 Degradation: Implications for the Pathogenesis of Preeclampsia"
  16. C. P. Chen, L. F. Chen, S. R. Yang, C. Y. Chen, C. C. Ko, G. D. Chang and H. Chen  (2008)  Biol Reprod  79, 815-823  "Functional Characterization of the Human Placental Fusogenic Membrane Protein Syncytin 2"
  17. M. H. Chiang, L. F. Chen and H. Chen  (2008)  Biol Reprod  79, 914-920  "Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for F-Box and WD Repeat Domain Containing 2-Mediated Human Glial Cell Missing Homolog 1 Ubiquitination and Degradation"
  18. C. C. Chou, C. W. Chang, J. H. Liu, C. D. Hsiao and H. Chen  (2007)  J Biol Chem  282, 27239-27249  "Small Ubiquitin-like Modifier Modification Regulates the DNA Binding Activity of Glial Cell Missing Drosophila Homolog a"
  19. S. Wei, L. F. Lin, C. C. Yang, Y. C. Wang, G. D. Chang, H. Chen and C. S. Chen  (2007)  Molecular Pharmaceutics  72, 725-733  "Thiazolidinediones modulate the expression of beta-catenin and other cell-cycle regulatoryproteins by targeting the F-box proteins of Skp1-Cul1-F-box protein E3 ubiquitin ligase independently of peroxisome proliferator-activated receptor gamma."
  20. C. P. Chen, K. G. Wang, C. Y. Chen, C. Yu, H. C. Chuang and (H. Chen)  (2006)  BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY  113, 152-158  "Altered Placental Syncytin and Its Receptor ASCT2 Expression in Placental Development and Pre-eclampsia"
  21. H. C. Chuang, C. W. Chang, G. D. Chang, T. P. Yao and H. Chen  (2006)  Nucleic Acids Res  35, 1459-1469  "Histone Deacetylase 3 Binds to and Regulates the GCMa Transcription Factor"
  22. C. Lin, M. Lin and H. Chen  (2005)  Biochem Cell Biol  83, 188-195  "Biochemical Characterization of the Human Placental Transcription Factor GCMa/1"
  23. C. S. Yang, C. Yu, H. C. Chuang, C. W. Chang, G. D. Chang, T. P. Yao and H. Chen  (2005)  J Biol Chem  280, 10083-10090  "FBW2 Targets GCMa to the Ubiquitin-Proteasome Degradation System"
  24. C. W. Chang, H. C. Chuang, C. Yu, T. P. Yao and H. Chen  (2005)  Mol Cell Biol  25, 8401-8414  "Stimulation of GCMa Transcriptional Activity by Cyclic AMP/Protein Kinase A Signaling Is Attributed to CBP-Mediated Acetylation of GCMa"
  25. C. P. Chen, C. Y. Chen, Y. C. Yang, T. H. Su and H. Chen  (2004)  Placenta  25, 413-421  "Decreased placental GCM1 (glial cells missing) gene expression in pre-eclampsia"
  26. C. Chang, P. T. Chen, G. D. Chang, C. J. Huang and H. Chen  (2004)  Biol Reprod  71, 1956-1962  "Functional characterization of the placental fusogenic membrane protein syncytin"
  27. G. D. Chang, C. J. Chen, C. Y. Lin, H. C. Chen and H. Chen  (2003)  J Biotechnol  102, 61-71  "Improvement of glycosylation in insect cells with mammalian glycosyltransferases"
  28. C. Yu, K. Shen, M. Lin, P. Chen, C. Lin, G. D. Chang and H. Chen  (2002)  J Biol Chem  277, 50062-50068  "GCMa regulates the syncytin-mediated trophoblastic fusion"
  29. C. D. Wang, G. D. Chang, Y. K. Lee and H. Chen  (2001)  Biol Reprod  65, 1437-1443  "A functional composite cis-element for NF kappa b and RBJ kappa in the rat pregnancy-specific glycoprotein gene"
  30. H. Chen, G. D. Chang  (2001)  Electrophoresis  22, 1894-1899  "Simultaneous immunoblotting analysis with activity gel electrophoresis in a single polyacrylamide gel"
  31. H. Chen, Y. Chong and C. L. Liu  (2000)  Biochemistry  39, 1675-1682  "Active intracellular domain of Notch enhances transcriptional activation of CCAAT/enhancer binding protein beta on a rat pregnancy-specific glycoprotein gene"
  32. A. Zingone, H. Hiraiwa, C. J. Pan, B. Lin, H. Chen, J. M. Ward and J. Y. Chou  (2000)  J Biol Chem  275, 828-832  "Correction of glycogen storage disease type 1a in a mouse model by gene therapy"
  33. Y. Chong, H. Chen  (2000)  Biotechniques  29, 1166-1167  "Preparation of functional recombinant protein from E. coli using a nondetergent sulfobetaine"
  34. S. H. Lin, H. Cheng, K. Earley, W. Luo and J. Chou  (1998)  Biochemical and Biophysical Research Communications  245, 472-477  "Demonstration of adhesion activity of the soluble Ig-domain protein C-CAM4 by attachment to the plasma membrane"
  35. C. J. Pan, K. J. Lei, H. Chen, J. M. Ward and J. Y. Chou  (1998)  Arch Biochem Biophys  358, 17-24  "Ontogeny of the murine glucose-6-phosphatase system"
  36. K. J. Lei, H. Chen, C. J. Pan, J. M. Ward, B. Mosinger, Jr., E. J. Lee, H. Westphal, B. C. Mansfield and J. Y. Chou  (1996)  Nat Genet  13, 203-209  "Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse"
  37. G. Chen, C. T. Guy, H. W. Chen, N. Hu, E. Y. Lee and W. H. Lee  (1996)  J Biol Chem  271, 9567-9572  "Molecular cloning and developmental expression of mouse p130, a member of the retinoblastoma gene family"

The research goal of my laboratory aims at unraveling the molecular mechanism underlying the differentiation of placental trophoblast cell, which is the first differentiated cell lineage during embryogenesis and is essential for implantation and placentation. Specifically, we study how trophoblast cells undergo cell fusion to form a multinucleated cell layer, the syncytiotrophoblast layer, which mediates gas and nutrient exchange between fetus and mother and produces growth factors and hormones essential for pregnancy maintenance. Recently, we have demonstrated that the placental transcription factor GCMa mediates trophoblastic fusion by upregulating expression of the placental fusogenic protein syncytin and that a decreased expression of GCMa gene is associated with the common pregnancy disorder pre-eclampsia. We have further demonstrated that GCMa activity is controlled by different post-translational modifications. The F-box protein FBW2 recognizes and promotes GCMa ubiquitination for proteasome degradation. On the contrary, the cAMP/PKA signaling pathway stabilizes GCMa protein via its downstream factor CBP, which catalyze GCMa acetylation and protects GCMa from ubiquitination. In addition, GCMa undergoes sumoylation, which decreases the DNA-binding activity of GCMa. Overall, syncytiotrophoblast differentiation can be regulated by post-translational modification of GCMa. The undergoing projects are to study additional signaling pathways and protein modifications that regulate placental cell differentiation.

Positions Held
  • 2008 – present   Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2003 – 2008   Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 1996 – 2003   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica.
  • 1994 – 1996   Visiting Fellow , Human Genetics Branch, NICHD, NIH.
  • 1991 – 1994   Adjunct Scientist, Human Genetics Branch, NICHD, NIH.
Degrees
  • 1994   Ph.D., Physiology, University of Florida.
  • 1986   B.S., Zoology, National Taiwan University.
A novel cAMP/Epac1/CaMKI signaling cascade promotes GCM1 desumoylation and placental cell fusion.
Chang, C.W.; Chang, G.D.; Chen, H.
Mol. Cell. Biol. (2011)
Dual-specificity phosphatase 23 mediates GCM1 dephosphorylation and activation.
Lin, F.Y.; Chang, C.W.; Cheong, M.L.; Chen, H.C.; Lee, D.Y.; Chang, G.D.; Chen, H.
Nucleic Acids Res. (2011)
GCM1 Regulation of the Expression of Syncytin-2 and Its Cognate Receptor MFSD2A in Placenta.
Liang, C.Y.; Wang, L.J.; Chen, C.P.; Chen, L.F.; Chen, Y.H.; Chen, H.
Biol. Reprod. (2010)
Gcm Protein Degradation Suppresses Proliferation of Glial Progenitors.
Ho, M.; Chen, H.; Chen, M.; Jacques, C.; Giangrande, A.; and Chien, C.T.
Proc. Natl. Acad. Sci. USA (2009)
Mechanism of Hypoxia-Induced GCM1 Degradation: Implications for the Pathogenesis of Preeclampsia.
J. Biol. Chem. (2009)
Functional Characterization of the Human Placental Fusogenic Membrane Protein Syncytin 2.
Chen, C.P.; Chen, L.F.; Yang, S.R.; Chen, C.Y.; Ko, C.C.; Chang, G.D.; Chen, H.
Biol. Reprod. (2008)
Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for F-Box and WD Repeat Domain Containing 2-Mediated Human Glial Cell Missing Homolog 1 Ubiquitination and Degradation.
Chiang, M.H.; Chen, L.F.; Chen, H.
Biol. Reprod. (2008)
Small Ubiquitin-like Modifier Modification Regulates the DNA Binding Activity of Glial Cell Missing Drosophila Homolog a.
Chou, C.C.; Chang, C.W.; Liu J.H.; Hsiao, C.D.; Chen, H.
J. Biol. Chem. (2007)
Thiazolidinediones modulate the expression of beta-catenin and other cell-cycle regulatoryproteins by targeting the F-box proteins of Skp1-Cul1-F-box protein E3 ubiquitin ligase independently of peroxisome proliferator-activated receptor gamma.
Wei, S.; Lin, L.F.; Yang, C.C.; Wang, Y.C.; Chang, G.D.; Chen, H.; Chen, C.S.
Mol. Pharm. (2007)
Histone Deacetylase 3 Binds to and Regulates the GCMa Transcription Factor.
Chuang, H.C.; Chang, C.W.; Chang, G.D.; Yao, T.P.; Chen, H.
Nucleic Acids Res. (2006)
  1. Wang LJ, Lo HF, Lin CF, Ng PS, Wu YH, Lee YS, Cheong ML, and (Chen H)*  (2019-01)  FASEB JOURNAL  33(1), 314-326  "SFRP3 Negatively Regulates Placental Extravillous Trophoblast Cell Migration Mediated by the GCM1-WNT10B-FZD7 Axis."
  2. Chiu YH, Yang MR, Wang LJ, Chen MH, Chang GD, (Chen H)*  (2018-06)  JOURNAL OF BIOLOGICAL CHEMISTRY  293(25), 9801-9811  "New Insights into the Regulation of Placental Growth Factor Gene Expression by the Transcription Factors GCM1 and DLX3 in Human Placenta."
  3. Lo, H.-F., Tsai, C.-Y., Chen, C.-P., Wang, L.-J., Lee, Y.-S., Chen, C.-Y., Liang, C.-T., Cheong, M.-L., (Chen, H.*)  (2017-02)  The Journal of pathology  241, 324-336  "Association of Dysfunctional Synapse Defective 1 (SYDE1) with Restricted Fetal Growth – SYDE1 Regulates Placental Cell Migration and Invasion"
  4. Chiu, Y. H. and (Chen, H.)  (2016-02)  Scientific Reports  6, 21630  "GATA3 Inhibits GCM1 Activity and Trophoblast Cell Invasion"
  5. Cheong ML, Wang LJ, Chuang PY, Chang CW, Lee YS, Lo HF, Tsai MS, (Chen H)  (2016-01)  Mol Cell Biol  36, 197-209  "A Positive Feedback Loop between Glial Cells Missing 1 (GCM1) and Human Chorionic Gonadotropin (hCG) Regulates Placental hCGbeta Expression and Cell Differentiation"
  6. Chen, Y.-Y., Chuang, P.-Y., Chen, C.-P., Chiu, Y.-H., Lo, H.-F., Cheong, M.-L., Huang, J.-Y., Kuo, P.-L., and (Chen, H.*)  (2014)  J Biol Chem  289(33), 22958-22968  "Functional Antagonism between High-temperature Requirement Protein A (HtrA) Family Members Regulates Trophoblast Invasion"
  7. Chang, C.-W., Cheong, M.-L., Chang, G.-D., Tsai, M.-S., and (Chen, H.*)  (2013-11)  Mol Hum Reprod  19(11), 745-755  "Involvement of Epac1/Rap1/CaMKI/HDAC5 Signaling Cascade in the Regulation of Placental Cell Fusion"
  8. Wu, Y.-H., Lo, H.-F., Chen, S.-H., and (Chen, H.)*  (2013-07)  FASEB J  27(7), 2818-2828  "Caspase-14 Suppresses GCM1 Acetylation and Inhibits Placental Cell Differentiation"
  9. Wang, C.-C., Lo, H.-F., Lin, S.-Y., and (Chen, H.*)  (2013)  Biochem J  453, 201-208  "RACK1 (Receptor for Activated C-kinase 1) Interacts with FBW2 (F-box and WD-repeat domain-containing 2) to Up-regulate GCM1 (Glial Cell Missing 1) Stability and Placental Cell Migration and Invasion"
  10. Wang, L.-J., Cheong, M.-L., Lee, Y.-S., Lee, M.-T., and (Chen, H.*)  (2012-09)  Mol Cell Biol  32(18), 3707-3717  "High-temperature Requirement A4 (HtrA4) Suppresses the Fusogenic Activity of Syncytin-1 and Promotes Trophoblast Invasion"
  11. Chang, C.-W., Chang, G.-D, and (Chen, H*)   (2011-09)  Mol Cell Biol  31(18), 3820-3831  "A Novel cAMP/Epac1/CaMKI Signaling Cascade Promotes GCM1 Desumoylation and Placental Cell Fusion"
  12. Lin, F.-Y., Chang, C.-W., Cheong, M.-L., Chen, H.-C., Lee, D.-Y., Chang, G.-D., and (Chen, H*)  (2011-02)  Nucleic Acids Res  39(3), 848-861  "Dual-specificity Phosphatase 23 Mediates GCM1 Dephosphorylation and Activation"
  13. Liang, C.-Y., Wang, L.-J., Chen, C.-P., Chen, L.-F., Chen, Y.-H., and (Chen, H*)  (2010)  Biol Reprod  83(3), 387-395  "GCM1 Regulation of the Expression of Syncytin 2 and Its Cognate Receptor MFSD2A in Human Placenta"
  14. Ho MS, (Chen H), Chen M, Jacques C, Giangrande A, Chien CT  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(16), 6778-6783  "Gcm Protein Degradation Suppresses Proliferation of Glial Progenitors"
  15. Chiang MH, Liang FY, Chen CP, Chang CW, Cheong ML, Wang LJ, Liang CY, Lin FY, Chou CC, (Chen H)  (2009)  J Biol Chem  284(26), 17411-17419  "Mechanism of Hypoxia-Induced GCM1 Degradation: Implications for the Pathogenesis of Preeclampsia"
  16. C. P. Chen, L. F. Chen, S. R. Yang, C. Y. Chen, C. C. Ko, G. D. Chang and H. Chen  (2008)  Biol Reprod  79, 815-823  "Functional Characterization of the Human Placental Fusogenic Membrane Protein Syncytin 2"
  17. M. H. Chiang, L. F. Chen and H. Chen  (2008)  Biol Reprod  79, 914-920  "Ubiquitin-Conjugating Enzyme UBE2D2 Is Responsible for F-Box and WD Repeat Domain Containing 2-Mediated Human Glial Cell Missing Homolog 1 Ubiquitination and Degradation"
  18. C. C. Chou, C. W. Chang, J. H. Liu, C. D. Hsiao and H. Chen  (2007)  J Biol Chem  282, 27239-27249  "Small Ubiquitin-like Modifier Modification Regulates the DNA Binding Activity of Glial Cell Missing Drosophila Homolog a"
  19. S. Wei, L. F. Lin, C. C. Yang, Y. C. Wang, G. D. Chang, H. Chen and C. S. Chen  (2007)  Molecular Pharmaceutics  72, 725-733  "Thiazolidinediones modulate the expression of beta-catenin and other cell-cycle regulatoryproteins by targeting the F-box proteins of Skp1-Cul1-F-box protein E3 ubiquitin ligase independently of peroxisome proliferator-activated receptor gamma."
  20. C. P. Chen, K. G. Wang, C. Y. Chen, C. Yu, H. C. Chuang and (H. Chen)  (2006)  BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY  113, 152-158  "Altered Placental Syncytin and Its Receptor ASCT2 Expression in Placental Development and Pre-eclampsia"
  21. H. C. Chuang, C. W. Chang, G. D. Chang, T. P. Yao and H. Chen  (2006)  Nucleic Acids Res  35, 1459-1469  "Histone Deacetylase 3 Binds to and Regulates the GCMa Transcription Factor"
  22. C. Lin, M. Lin and H. Chen  (2005)  Biochem Cell Biol  83, 188-195  "Biochemical Characterization of the Human Placental Transcription Factor GCMa/1"
  23. C. S. Yang, C. Yu, H. C. Chuang, C. W. Chang, G. D. Chang, T. P. Yao and H. Chen  (2005)  J Biol Chem  280, 10083-10090  "FBW2 Targets GCMa to the Ubiquitin-Proteasome Degradation System"
  24. C. W. Chang, H. C. Chuang, C. Yu, T. P. Yao and H. Chen  (2005)  Mol Cell Biol  25, 8401-8414  "Stimulation of GCMa Transcriptional Activity by Cyclic AMP/Protein Kinase A Signaling Is Attributed to CBP-Mediated Acetylation of GCMa"
  25. C. P. Chen, C. Y. Chen, Y. C. Yang, T. H. Su and H. Chen  (2004)  Placenta  25, 413-421  "Decreased placental GCM1 (glial cells missing) gene expression in pre-eclampsia"
  26. C. Chang, P. T. Chen, G. D. Chang, C. J. Huang and H. Chen  (2004)  Biol Reprod  71, 1956-1962  "Functional characterization of the placental fusogenic membrane protein syncytin"
  27. G. D. Chang, C. J. Chen, C. Y. Lin, H. C. Chen and H. Chen  (2003)  J Biotechnol  102, 61-71  "Improvement of glycosylation in insect cells with mammalian glycosyltransferases"
  28. C. Yu, K. Shen, M. Lin, P. Chen, C. Lin, G. D. Chang and H. Chen  (2002)  J Biol Chem  277, 50062-50068  "GCMa regulates the syncytin-mediated trophoblastic fusion"
  29. C. D. Wang, G. D. Chang, Y. K. Lee and H. Chen  (2001)  Biol Reprod  65, 1437-1443  "A functional composite cis-element for NF kappa b and RBJ kappa in the rat pregnancy-specific glycoprotein gene"
  30. H. Chen, G. D. Chang  (2001)  Electrophoresis  22, 1894-1899  "Simultaneous immunoblotting analysis with activity gel electrophoresis in a single polyacrylamide gel"
  31. H. Chen, Y. Chong and C. L. Liu  (2000)  Biochemistry  39, 1675-1682  "Active intracellular domain of Notch enhances transcriptional activation of CCAAT/enhancer binding protein beta on a rat pregnancy-specific glycoprotein gene"
  32. A. Zingone, H. Hiraiwa, C. J. Pan, B. Lin, H. Chen, J. M. Ward and J. Y. Chou  (2000)  J Biol Chem  275, 828-832  "Correction of glycogen storage disease type 1a in a mouse model by gene therapy"
  33. Y. Chong, H. Chen  (2000)  Biotechniques  29, 1166-1167  "Preparation of functional recombinant protein from E. coli using a nondetergent sulfobetaine"
  34. S. H. Lin, H. Cheng, K. Earley, W. Luo and J. Chou  (1998)  Biochemical and Biophysical Research Communications  245, 472-477  "Demonstration of adhesion activity of the soluble Ig-domain protein C-CAM4 by attachment to the plasma membrane"
  35. C. J. Pan, K. J. Lei, H. Chen, J. M. Ward and J. Y. Chou  (1998)  Arch Biochem Biophys  358, 17-24  "Ontogeny of the murine glucose-6-phosphatase system"
  36. K. J. Lei, H. Chen, C. J. Pan, J. M. Ward, B. Mosinger, Jr., E. J. Lee, H. Westphal, B. C. Mansfield and J. Y. Chou  (1996)  Nat Genet  13, 203-209  "Glucose-6-phosphatase dependent substrate transport in the glycogen storage disease type-1a mouse"
  37. G. Chen, C. T. Guy, H. W. Chen, N. Hu, E. Y. Lee and W. H. Lee  (1996)  J Biol Chem  271, 9567-9572  "Molecular cloning and developmental expression of mouse p130, a member of the retinoblastoma gene family"