Research

Post-translational modification (PTM) on histones plays important roles in regulating all DNA-dependent processes, including transcription and replication, by modulating the accessibility of DNA and recruiting protein factors to chromatin. PTMs on histones include phosphorylation, ubiquitination, ADP-ribosylation, O-GlcNAcylation, methylation and acetylation. The acetylation of lysine residues in histones is catalyzed by enzymes called histone acetyltransferases (HATs). Other than histones, many non-histone substrates have also been identified for HATs, adding another layer of complexity to their biological roles. Our lab is interested in understanding the functions of HATs and the mechanisms underlying their roles, and we are currently focusing on the Drosophila HAT Enok. Enok is a homolog of human MOZ, a key regulator of hematopoiesis and involved in leukemia, and is important for neuroblast proliferation and stem cell maintenance. Given that the protein complexes formed by Enok/MOZ are highly conserved between Drosophila and human, we are interested in using Drosophila as a simple and great genetic model to dissect the regulations and roles of Enok/MOZ.

Currently, we are pursuing two topics:

  1. The roles of Enok in cell cycle regulation through acetylating non-histone targets.
  2. The transcriptional roles of Enok in germline stem cell maintenance.
Degrees and Positions Held
Positions Held
  • 2015 – present   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2011 – 2015   Postdoctoral Research Associate, Stowers Institute for Medical Research
Degrees
  • 2005 – 2011   Ph.D., Biochemistry, Vanderbilt University School of Medicine
  • 2001 – 2005   B.S., Life Science, National Tsing Hua University
Selected Publications
Huang F, Abmayr SM, Workman JL
Mol Cell Biol (2016)
Huang F, Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL
Genes Dev (2016)
Huang F, Ramakrishnan S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Bhaskara S, Cairns BR, Chandrasekharan MB
J Biol Chem (2015)
Huang F, Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL
Genes Dev (2014)
Huang F, Workman JL
Cell Res (2013)
Chandrasekharan MB, Huang F, Sun ZW
Methods (2011)
Chandrasekharan MB, Huang F, Chen YC, Sun ZW
Mol Cell Biol (2010)
Chandrasekharan MB, Huang F, Sun ZW
Epigenetics (2010)
Huang F, Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW
J Biol Chem (2010)
Chandrasekharan MB, Huang F, Sun ZW
Proc Natl Acad Sci U S A (2009)
Publications List
  1. Rastegari E, Kajal K, Tan BS, (Huang F), Chen RH, Hsieh TS, Hsu HJ  (2020-01)  Development  147(2), dev182063  "WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila."
  2. Lai CM, Lin KY, Kao SH, Chen YN, (Huang F), Hsu HJ  (2017-05)  The Journal of cell biology  216(5), 1439-1453  "Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion."
  3. (Huang F), Abmayr SM, Workman JL  (2016-06)  Molecular and cellular biology  36(14), 1900-1907  "Regulation of KAT6 Acetyltransferases and Their Roles in Cell Cycle Progression, Stem Cell Maintenance, and Human Disease."
  4. (Huang F), Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL  (2016-05)  Genes & development  30(10), 1198-1210  "The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition."
  5. (Huang F), Abmayr SM, Workman JL  (2016)  Cell Cycle  15(22), 3001-3002  "Limiting PCNA-unloading at the G1/S transition."
  6. (Huang F), Ramakrishnan S, Pokhrel S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Cairns BR, Bhaskara S, Chandrasekharan MB  (2015)  The Journal of biological chemistry  290(48), 28760-28777  "Interaction of the Jhd2 Histone H3 Lys-4 Demethylase with Chromatin Is Controlled by Histone H2A Surfaces and Restricted by H2B Ubiquitination."
  7. (Huang F), Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL  (2014)  Genes & development  28(24), 2750-2763  "Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire."
  8. (Huang F), Workman JL  (2013)  Cell research  23(10), 1153-1154  "Directing transcription to the right way."
  9. Chandrasekharan MB, (Huang F), Sun ZW  (2011)  Methods  54(3), 304-314  "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors."
  10. Chandrasekharan MB, (Huang F), Chen YC, Sun ZW  (2010)  Molecular and cellular biology  30(13), 3216-3232  "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination."
  11. Chandrasekharan MB, (Huang F), Sun ZW  (2010)  Epigenetics  5(6), 460-468  "Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation."
  12. (Huang F), Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW  (2010)  The Journal of biological chemistry  285(32), 24548-24561  "The JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation."
  13. Chandrasekharan MB, (Huang F), Sun ZW  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(39), 16686-16691  "Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability."
  14. Chen WY, Lee WC, Hsu NC, (Huang F), Chung BC  (2004)  The Journal of biological chemistry  279(37), 38730-38735  "SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1)."

Post-translational modification (PTM) on histones plays important roles in regulating all DNA-dependent processes, including transcription and replication, by modulating the accessibility of DNA and recruiting protein factors to chromatin. PTMs on histones include phosphorylation, ubiquitination, ADP-ribosylation, O-GlcNAcylation, methylation and acetylation. The acetylation of lysine residues in histones is catalyzed by enzymes called histone acetyltransferases (HATs). Other than histones, many non-histone substrates have also been identified for HATs, adding another layer of complexity to their biological roles. Our lab is interested in understanding the functions of HATs and the mechanisms underlying their roles, and we are currently focusing on the Drosophila HAT Enok. Enok is a homolog of human MOZ, a key regulator of hematopoiesis and involved in leukemia, and is important for neuroblast proliferation and stem cell maintenance. Given that the protein complexes formed by Enok/MOZ are highly conserved between Drosophila and human, we are interested in using Drosophila as a simple and great genetic model to dissect the regulations and roles of Enok/MOZ.

Currently, we are pursuing two topics:

  1. The roles of Enok in cell cycle regulation through acetylating non-histone targets.
  2. The transcriptional roles of Enok in germline stem cell maintenance.
Positions Held
  • 2015 – present   Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2011 – 2015   Postdoctoral Research Associate, Stowers Institute for Medical Research
Degrees
  • 2005 – 2011   Ph.D., Biochemistry, Vanderbilt University School of Medicine
  • 2001 – 2005   B.S., Life Science, National Tsing Hua University
Huang F, Abmayr SM, Workman JL
Mol Cell Biol (2016)
Huang F, Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL
Genes Dev (2016)
Huang F, Ramakrishnan S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Bhaskara S, Cairns BR, Chandrasekharan MB
J Biol Chem (2015)
Huang F, Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL
Genes Dev (2014)
Huang F, Workman JL
Cell Res (2013)
Chandrasekharan MB, Huang F, Sun ZW
Methods (2011)
Chandrasekharan MB, Huang F, Chen YC, Sun ZW
Mol Cell Biol (2010)
Chandrasekharan MB, Huang F, Sun ZW
Epigenetics (2010)
Huang F, Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW
J Biol Chem (2010)
Chandrasekharan MB, Huang F, Sun ZW
Proc Natl Acad Sci U S A (2009)
  1. Rastegari E, Kajal K, Tan BS, (Huang F), Chen RH, Hsieh TS, Hsu HJ  (2020-01)  Development  147(2), dev182063  "WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila."
  2. Lai CM, Lin KY, Kao SH, Chen YN, (Huang F), Hsu HJ  (2017-05)  The Journal of cell biology  216(5), 1439-1453  "Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion."
  3. (Huang F), Abmayr SM, Workman JL  (2016-06)  Molecular and cellular biology  36(14), 1900-1907  "Regulation of KAT6 Acetyltransferases and Their Roles in Cell Cycle Progression, Stem Cell Maintenance, and Human Disease."
  4. (Huang F), Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM, Workman JL  (2016-05)  Genes & development  30(10), 1198-1210  "The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition."
  5. (Huang F), Abmayr SM, Workman JL  (2016)  Cell Cycle  15(22), 3001-3002  "Limiting PCNA-unloading at the G1/S transition."
  6. (Huang F), Ramakrishnan S, Pokhrel S, Pflueger C, Parnell TJ, Kasten MM, Currie SL, Bhachech N, Horikoshi M, Graves BJ, Cairns BR, Bhaskara S, Chandrasekharan MB  (2015)  The Journal of biological chemistry  290(48), 28760-28777  "Interaction of the Jhd2 Histone H3 Lys-4 Demethylase with Chromatin Is Controlled by Histone H2A Surfaces and Restricted by H2B Ubiquitination."
  7. (Huang F), Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM, Workman JL  (2014)  Genes & development  28(24), 2750-2763  "Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire."
  8. (Huang F), Workman JL  (2013)  Cell research  23(10), 1153-1154  "Directing transcription to the right way."
  9. Chandrasekharan MB, (Huang F), Sun ZW  (2011)  Methods  54(3), 304-314  "Decoding the trans-histone crosstalk: methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors."
  10. Chandrasekharan MB, (Huang F), Chen YC, Sun ZW  (2010)  Molecular and cellular biology  30(13), 3216-3232  "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination."
  11. Chandrasekharan MB, (Huang F), Sun ZW  (2010)  Epigenetics  5(6), 460-468  "Histone H2B ubiquitination and beyond: Regulation of nucleosome stability, chromatin dynamics and the trans-histone H3 methylation."
  12. (Huang F), Chandrasekharan MB, Chen YC, Bhaskara S, Hiebert SW, Sun ZW  (2010)  The Journal of biological chemistry  285(32), 24548-24561  "The JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation."
  13. Chandrasekharan MB, (Huang F), Sun ZW  (2009)  Proceedings of the National Academy of Sciences of the United States of America  106(39), 16686-16691  "Ubiquitination of histone H2B regulates chromatin dynamics by enhancing nucleosome stability."
  14. Chen WY, Lee WC, Hsu NC, (Huang F), Chung BC  (2004)  The Journal of biological chemistry  279(37), 38730-38735  "SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1)."