Research

Human hPuf-A/KIAA0020 was first identified as a new minor histocompatibility antigen in 2001. Its zebrafish ortholog contains six Pumilio-homology RNA-binding domains and has been shown to participate in the development of eyes and primordial germ cells, but the cellular function of hPuf-A remains unclear. In this report, we showed that hPuf-A predominantly localized in the nucleoli with minor punctate signals in the nucleoplasm. The nucleolar localization of hPuf-A would redistribute to the nucleoplasm after the treatment of RNA polymerase inhibitors (actinomycin D and 5,6-dichlorobenzimidazole riboside) and topoisomerase inhibitors [camptothecin (CPT) and etoposide]. Interestingly, knockdown of hPuf-A sensitized cells to CPT and UV treatment and cells constitutively overexpressing hPuf-A became more resistant to genotoxic exposure. Affinity gel pull-down coupled with mass spectrometric analysis identified PARP-1 as an hPuf-A-interacting protein. hPuf-A specifically interacts with the catalytic domain of PARP-1 and inhibits poly(ADPribosyl)ation of PARP-1 in vitro. Depletion of hPuf-A increased the cleaved PARP-1 and overexpression of hPuf-A lessened PARP-1 cleavage when cells were exposed to CPT and UV light. Collectively, hPuf-A may regulate cellular response to genotoxic stress by inhibiting PARP-1 activity and thus preventing PARP-1 degradation by caspase-3.

Degrees and Positions Held
Positions Held
  • 2012 – present   Associate Professor, Inst. Biochem. Sciences, National Taiwan University
  • 2008 – present   Joint Appointment Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2008 – 2012   Assistant Professor, Inst. Biochem. Sciences, National Taiwan University
  • 2005 – 2008   Investigator, Dept. of Medical Research, Mackay Memorial Hospita
  • 1999 – 2004   Associate Investigator, Dept. of Medical Research, Mackay Memorial Hospital
  • 1997 – 1999   Postdoc fellow, Dana-Farber Cancer Institute, Harvard Medical School
Degrees
  • 1993 – 1997   Ph.D., Department of Zoology, National Taiwan University
  • 1992 – 1993   M.S., Department of Zoology, National Taiwan University
  • 1985 – 1989   B.S., Department of Zoology, National Taiwan University
Selected Publications
Chen YL, Jiang YW, Su YL, Lee SC, Chang MS, Chang CJ
Mol. Biol. Rep. (2013)
Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, Chang MS
Tumor Biol. (2013)
Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, Chang MS
PloS one (2012)
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research (2011)
Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS
Carcinogenesis (2009)
Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR
The Journal of biological chemistry (2008)
Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.
Blood (2007)
Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS
Biochemical and biophysical research communications (2006)
Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS
Cancer research (2006)
Publications List
  1. Fan JJ, Hsu WH, Lee KH, Chen KC, Lin CW, Lee YA, Ko TP, Lee LT, Lee MT, (Chang MS), Cheng CH (2019-11) Antioxidants 8(11), E557 “”Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.”
  2. Lee JY, Lee LJ, Fan CC, Chang HC, Shih HA, Min MY, (Chang MS) (2017-04) Scientific Reports 7, 45646 “Important roles of Vilse in dendritic architecture and synaptic plasticity.”
  3. Su WP, Hsu SH, Chia LC, Lin JY, Chang SB, Jiang ZD, Lin YJ, Shih MY, Chen YC, Chang MS, Yang WB, Hung JJ, Hung PC, Wu WS, Myung K, Liaw H (2016-01) Genetics 202(1), 77-92 “Combined Interactions of Plant Homeodomain and Chromodomain Regulate NuA4 Activity at DNA Double-Strand Breaks.”
  4. Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, (Chang MS)  (2015-04)  Cell death & disease  6, e1716  “PHRF1 promotes genome integrity by modulating non-homologous end-joining.”
  5. Cheng CH, Chou CM, Chu CY, Chen GD, Lien HW, Hwang PP, (Chang MS), Huang CJ  (2014)  Fish & shellfish immunology  38(1), 230-43  “Differential regulation of Tetraodon nigroviridis Mx gene promoter activity by constitutively-active forms of STAT1, STAT2, and IRF9.”
  6. Zhong W, Zhou Y, Li J, Mysore R, Luo W, Li S, (Chang MS), Olkkonen VM, Yan D  (2014)  Experimental cell research  322(2), 227-235  “OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.”
  7. Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, (Chang MS)  (2013-10)  TUMOR BIOLOGY  34(5), 2557-2564  “Upregulated hPuf-A promotes breast cancer tumorigenesis.”
  8. Chen YL, Jiang YW, Su YL, Lee SC, (Chang MS), Chang CJ  (2013-04)  Molecular biology reports  40(4), 2867-2877  “Transcriptional regulation of tristetraprolin by NF-kappaB signaling in LPS-stimulated macrophages.”
  9. Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, (Chang MS)  (2012)  PloS one  7(3), e33538  “Disruption of Murine mp29/Syf2/Ntc31 Gene Results in Embryonic Lethality with Aberrant Checkpoint Response.”
  10. Yeh PA, Yang WH, Chiang PY, Wang SC, (Chang MS), Chang CJ  (2012)  International journal of biological sciences  8(5), 606-19  “Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11.”
  11. Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, (Chang MS)  (2011-02)  Cancer research  71(3), 1126-34  “hPuf-A/KIAA0020 Modulates PARP-1 Cleavage upon Genotoxic Stress.”
  12. Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS  (2009-10)  Carcinogenesis  30(10), 1710-1716  “Involvement of p29 in DNA damage responses and Fanconi anemia pathway.”
  13. Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR  (2008)  The Journal of biological chemistry  283(4), 2454-64  “Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.”
  14. Cheng TS, Hsiao YL, Lin CC, Hsu CM, Chang MS, Lee CI, Yu RC, Huang CY, Howng SL, Hong YR  (2007)  Experimental cell research  313(8), 1710-21  “hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases.”
  15. Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.  (2007)  Blood  110(13), 4526-4534  “I branching formation in erythroid differentiation is regulated by transcription factor C/EBPα”
  16. Ho HT, Chang MS, Wei TY, Hsieh WS, Hung CC, Yang HM, Lu YT  (2006)  Chest  129(1), 95-101  “Colonization of severe acute respiratory syndrome-associated coronavirus among health-care workers screened by nasopharyngeal swab.”
  17. Chan WE, Chuang CK, Yeh SH, Chang MS, Chen SS  (2006)  Journal of virology  80(7), 3225-37  “Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus.”
  18. Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS  (2006)  Biochemical and biophysical research communications  343(2), 428-34  “Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.”
  19. Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS  (2006)  Cancer research  66(17), 8484-91  “Silencing of p29 affects DNA damage responses with UV irradiation.”
  20. Chang MS, Yang YC, Kuo YC, Kuo YH, Chang C, Chen CM, Lee TH  (2005)  Journal of natural products  68(1), 11-3  “Furocoumarin glycosides from the leaves of Ficus ruficaulis Merr. var. antaoensis.”

Human hPuf-A/KIAA0020 was first identified as a new minor histocompatibility antigen in 2001. Its zebrafish ortholog contains six Pumilio-homology RNA-binding domains and has been shown to participate in the development of eyes and primordial germ cells, but the cellular function of hPuf-A remains unclear. In this report, we showed that hPuf-A predominantly localized in the nucleoli with minor punctate signals in the nucleoplasm. The nucleolar localization of hPuf-A would redistribute to the nucleoplasm after the treatment of RNA polymerase inhibitors (actinomycin D and 5,6-dichlorobenzimidazole riboside) and topoisomerase inhibitors [camptothecin (CPT) and etoposide]. Interestingly, knockdown of hPuf-A sensitized cells to CPT and UV treatment and cells constitutively overexpressing hPuf-A became more resistant to genotoxic exposure. Affinity gel pull-down coupled with mass spectrometric analysis identified PARP-1 as an hPuf-A-interacting protein. hPuf-A specifically interacts with the catalytic domain of PARP-1 and inhibits poly(ADPribosyl)ation of PARP-1 in vitro. Depletion of hPuf-A increased the cleaved PARP-1 and overexpression of hPuf-A lessened PARP-1 cleavage when cells were exposed to CPT and UV light. Collectively, hPuf-A may regulate cellular response to genotoxic stress by inhibiting PARP-1 activity and thus preventing PARP-1 degradation by caspase-3.

Positions Held
  • 2012 – present   Associate Professor, Inst. Biochem. Sciences, National Taiwan University
  • 2008 – present   Joint Appointment Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica
  • 2008 – 2012   Assistant Professor, Inst. Biochem. Sciences, National Taiwan University
  • 2005 – 2008   Investigator, Dept. of Medical Research, Mackay Memorial Hospita
  • 1999 – 2004   Associate Investigator, Dept. of Medical Research, Mackay Memorial Hospital
  • 1997 – 1999   Postdoc fellow, Dana-Farber Cancer Institute, Harvard Medical School
Degrees
  • 1993 – 1997   Ph.D., Department of Zoology, National Taiwan University
  • 1992 – 1993   M.S., Department of Zoology, National Taiwan University
  • 1985 – 1989   B.S., Department of Zoology, National Taiwan University
Chen YL, Jiang YW, Su YL, Lee SC, Chang MS, Chang CJ
Mol. Biol. Rep. (2013)
Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, Chang MS
Tumor Biol. (2013)
Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, Chang MS
PloS one (2012)
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research (2011)
Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS
Carcinogenesis (2009)
Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR
The Journal of biological chemistry (2008)
Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.
Blood (2007)
Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS
Biochemical and biophysical research communications (2006)
Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS
Cancer research (2006)
  1. Fan JJ, Hsu WH, Lee KH, Chen KC, Lin CW, Lee YA, Ko TP, Lee LT, Lee MT, (Chang MS), Cheng CH (2019-11) Antioxidants 8(11), E557 “”Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.”
  2. Lee JY, Lee LJ, Fan CC, Chang HC, Shih HA, Min MY, (Chang MS) (2017-04) Scientific Reports 7, 45646 “Important roles of Vilse in dendritic architecture and synaptic plasticity.”
  3. Su WP, Hsu SH, Chia LC, Lin JY, Chang SB, Jiang ZD, Lin YJ, Shih MY, Chen YC, Chang MS, Yang WB, Hung JJ, Hung PC, Wu WS, Myung K, Liaw H (2016-01) Genetics 202(1), 77-92 “Combined Interactions of Plant Homeodomain and Chromodomain Regulate NuA4 Activity at DNA Double-Strand Breaks.”
  4. Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, (Chang MS)  (2015-04)  Cell death & disease  6, e1716  “PHRF1 promotes genome integrity by modulating non-homologous end-joining.”
  5. Cheng CH, Chou CM, Chu CY, Chen GD, Lien HW, Hwang PP, (Chang MS), Huang CJ  (2014)  Fish & shellfish immunology  38(1), 230-43  “Differential regulation of Tetraodon nigroviridis Mx gene promoter activity by constitutively-active forms of STAT1, STAT2, and IRF9.”
  6. Zhong W, Zhou Y, Li J, Mysore R, Luo W, Li S, (Chang MS), Olkkonen VM, Yan D  (2014)  Experimental cell research  322(2), 227-235  “OSBP-related protein 8 (ORP8) interacts with Homo sapiens sperm associated antigen 5 (SPAG5) and mediates oxysterol interference of HepG2 cell cycle.”
  7. Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, (Chang MS)  (2013-10)  TUMOR BIOLOGY  34(5), 2557-2564  “Upregulated hPuf-A promotes breast cancer tumorigenesis.”
  8. Chen YL, Jiang YW, Su YL, Lee SC, (Chang MS), Chang CJ  (2013-04)  Molecular biology reports  40(4), 2867-2877  “Transcriptional regulation of tristetraprolin by NF-kappaB signaling in LPS-stimulated macrophages.”
  9. Chen CH, Chu PC, Lee L, Lien HW, Lin TL, Fan CC, Chi P, Huang CJ, (Chang MS)  (2012)  PloS one  7(3), e33538  “Disruption of Murine mp29/Syf2/Ntc31 Gene Results in Embryonic Lethality with Aberrant Checkpoint Response.”
  10. Yeh PA, Yang WH, Chiang PY, Wang SC, (Chang MS), Chang CJ  (2012)  International journal of biological sciences  8(5), 606-19  “Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11.”
  11. Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, (Chang MS)  (2011-02)  Cancer research  71(3), 1126-34  “hPuf-A/KIAA0020 Modulates PARP-1 Cleavage upon Genotoxic Stress.”
  12. Chu PC, Wang TY, Lu YT, Chou CK, Yang YC, Chang MS  (2009-10)  Carcinogenesis  30(10), 1710-1716  “Involvement of p29 in DNA damage responses and Fanconi anemia pathway.”
  13. Cheng TS, Hsiao YL, Lin CC, Yu CT, Hsu CM, Chang MS, Lee CI, Huang CY, Howng SL, Hong YR  (2008)  The Journal of biological chemistry  283(4), 2454-64  “Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.”
  14. Cheng TS, Hsiao YL, Lin CC, Hsu CM, Chang MS, Lee CI, Yu RC, Huang CY, Howng SL, Hong YR  (2007)  Experimental cell research  313(8), 1710-21  “hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases.”
  15. Twu YC, Chen CP, Hsieh CY, Tzeng CH, Sun CF, Wang SF, Chang MS, Yu LC.  (2007)  Blood  110(13), 4526-4534  “I branching formation in erythroid differentiation is regulated by transcription factor C/EBPα”
  16. Ho HT, Chang MS, Wei TY, Hsieh WS, Hung CC, Yang HM, Lu YT  (2006)  Chest  129(1), 95-101  “Colonization of severe acute respiratory syndrome-associated coronavirus among health-care workers screened by nasopharyngeal swab.”
  17. Chan WE, Chuang CK, Yeh SH, Chang MS, Chen SS  (2006)  Journal of virology  80(7), 3225-37  “Functional characterization of heptad repeat 1 and 2 mutants of the spike protein of severe acute respiratory syndrome coronavirus.”
  18. Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS  (2006)  Biochemical and biophysical research communications  343(2), 428-34  “Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.”
  19. Chu PC, Yang YC, Lu YT, Chen HT, Yu LC, Chang MS  (2006)  Cancer research  66(17), 8484-91  “Silencing of p29 affects DNA damage responses with UV irradiation.”
  20. Chang MS, Yang YC, Kuo YC, Kuo YH, Chang C, Chen CM, Lee TH  (2005)  Journal of natural products  68(1), 11-3  “Furocoumarin glycosides from the leaves of Ficus ruficaulis Merr. var. antaoensis.”