Our research program is focused in the areas of biosynthetic chemistry (with a particular emphasis in enzymatic carbohydrate synthesis), the structure determination/analysis of oligosaccharides and the discovery of novel carbohydrate-active enzymes that can be used in complex oligosaccharide synthesis. Students and postdoctoral fellows in the group use synthetic chemistry and enzymatic strategy to prepare compounds with interesting biological activities and then have the opportunity to use them in either biochemical or conformational studies.

The long-term goals of our research program are to develop state-of-the-art methods for assembling complex, biologically-relevant carbohydrates (glycans) that can be used to probe their function in nature. My underlying research philosophy is to work on problems in which developing methods for preparing novel, structurally-interesting glycans leads to tools for studying vital biological problems. The major area of focus is human milk glycans. Specific systems of interest are: 1) Discovering new enzymes as biocatalysts for human milk-type glycan synthesis; 2) Chemoenzymatic synthesis of the human milk oligosaccharide library; and 3) The function of human milk oligosaccharides in human gut microbiota and to investigate their roles in preventing the pathogenic infections.

Positions Held

• 2022 – present   Associate Professor, Department of Chemistry, National Tsing Hua University
• 2021 – present   Joint Appointment Associate Research Fellow, Institute of Biological Chemistry, Academia Sinica
• 2020 – 2022   Associate Professor, Department of Chemistry and Biochemistry, National Chung Cheng University
• 2015 – 2020   Assistant Professor, Department of Chemistry and Biochemistry, National Chung Cheng University
• 2014 – 2015   Postdoc Research Fellow, Department of Chemistry, University of British Columbia, BC, Canada
• 2013 – 2014   Postdoc Research Fellow, Department of Chemistry, National Tsing Hua University

Degrees

• 2007 – 2012   Ph.D., Department of Chemistry, National Tsing Hua University
• 2005 – 2007   M.S., Department of Chemistry, National Tsing Hua University
• 2001 – 2005   B.S., Department of Chemistry, National Changhua University of Education

1. Ooi KE, Zhang XW, Kuo CY, Liu YJ, (Yu CC)* (2022-05) Frontiers in Chemistry 10, 905105 “Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.”
2. Pao YP, (Yu CC), Lin YZ, Chatterjee S, Saha S, Tiwari N, Huang YT, Wu CC*, Choi D*, Lin ZH* (2021-07) Nano Energy 85, 106008 “Carbohydrate-Protein Interactions Studied by Solid-Liquid Contact Electrification and its Use for Label-Free Bacterial Detection.”
3. Huang YT, Su YC, Wu HR, Huang HH, Lin EC, Tsai TW, Tseng HW, Fang JL, (Yu CC)* (2021-03) ACS Catalysis 11(5), 2631−2643 “Sulfo-Fluorous Tagging Strategy for Site-Selective Enzymatic Glycosylation of para-Human Milk Oligosaccharides.”
4. Liou SW, Fang JL, Lin HW, Tsai TW, Huang HH, Liang CY, Yang, CR, Wei GT*, (Yu CC)* (2021-03) Chemistry – An Asian Journal 16(5), 492–497 “Effective separation of human milk glycosides using carbon dioxide supercritical fluid chromatography.”
5. Huang HH, Fang JL, Wang HK, Sun CY, Tsai TW, Huang YT, Kuo CY, Wang YJ, Liao CC, (Yu CC)* (2019-12) ACS Catalysis 9(12), 11794−11800 “Substrate Characterization of Bacteroides fragilis α1,3/4-Fucosyltransferase Enabling Access to Programmable One-Pot Enzymatic Synthesis of KH 1 Antigen.”
6. Tsai TW, Fang JL, Liang CY, Wang CJ, Huang YT, Wang YJ, Li JY, (Yu CC)* (2019-12) ACS Catalysis 9(12) 10712−10720 “Exploring the Synthetic Application of Helicobacter pylori α1,3/4-Fucosyltransferase FucTIII toward the Syntheses of Fucosylated Human Milk Glycans and Lewis Antigens.”
7. Zhang X, Chen F, Petrella A, Chacón-Huete F, Covone J, Tsai TW, (Yu CC), Forgione P, Kwan DH* (2019-04) ACS Chemical Biology 14(4), 715−724 “A High-Throughput Glycosyltransferase Inhibition Assay for Identifying Molecules Targeting Fucosylation in Cancer Cell-Surface Modification.”
8. Fang JL, Tsai TW, Liang CY, Li JY, (Yu CC)* (2018-09) Advanced Synthesis & Catalysis 360(17), 3213–3219 “Enzymatic Synthesis of Human Milk Fucosides α1,2‐Fucosyl para‐Lacto‐N‐Hexaose and its Isomeric Derivatives.”
9. Kuo CS, Kao CR, Chen WJ, Lu MY, Cullen DA, Sneed BT, Chuang YC, (Yu CC), Kuo CH* (2018-07) Chemistry of Materials 30(13), 4448–4458 “Aqueous Synthesis of Concave Rh Nanotetrahedra with Defect-Rich Surfaces: Insights into Growth-, Defect-, and Plasmon-Enhanced Catalytic Energy Conversion.”
10. Hou KL, Chiang PY, Lin CH, Li BY, Chien WT, Huang YT, (Yu CC)*, Lin CC* (2018-06) Advanced Synthesis & Catalysis 360(12), 2313–2323 “Water-soluble Sulfo-Fluorous Affinity (SOFA) Tag-Assisted Enzymatic Synthesis of Oligosaccharides.”
11. 方嘉琳 (Jia-Lin Fang); 蔡騰緯 (Teng-Wei Tsai); 游景晴* (Ching-Ching Yu), 化學季刊, 75 卷 3 期 (2017/09/01), 219–228. “岩藻糖化人類母乳寡糖的功能、合成到應用 (Fucosylated Human Milk Oligosaccharides: Function, Synthesis and Applications)”
12. Adak AK, (Yu CC), Lin CC*, (2016) Glycochemical Synthesis: Strategies and Applications Chapter 16 “Synthesis and Applications of Glyconanoparticles, Glycodendrimers, and Glycoclusters in Biological Systems” Hung SC and Zulueta MML Ed., John Wiley & Sons, Inc.
13. Volkers G, Worrall LJ, Kwan DH, (Yu CC), Baumann L, Lameignere E, Wasney GA, Scott NE, Wakarchuk W, Foster LJ, Withers SG, Strynadka NCJ* (2015-08) Nature Structural & Molecular Biology 22(8), 627–635 “Structure of Human ST8SiaIII Sialyltransferase Provides Insight into Cell-Surface Polysialylation.”
14. (Yu CC), Withers SG* (2015-05) Advanced Synthesis & Catalysis 357(8), 1633–1654 “Recent Developments in Enzymatic Synthesis of Modified Sialic Acid Derivatives.”
15. Huang LD, Adak AK, (Yu CC), Hsiao WC, Lin HJ, Chen ML, Lin CC* (2015-01) Chemistry – An European Journal 21(10), 3956–3967 “Fabrication of Highly Stable Glyco-Gold Nanoparticles and Development of a Glyco-Gold Nanoparticle-Based Oriented-Immobilized Antibody Microarray for Lectin (GOAL) Assay.”
16. Li SP, Hsiao WC, (Yu CC), Chien WT, Lin HJ, Huang LD, Lin CH, Wu WL, Wu SH, Lin CC* (2014-10) Advanced Synthesis & Catalysis 356(14-15) 3199–3213 “Characterization of Meiothermus Taiwanensis Galactokinase and its Uses in the One-Pot Enzymatic Synthesis of Uridine Diphosphate-Galactose and The Chemoenzymatic Synthesis of the Carbohydrate Antigen Stage Specific Embryonic Antigen-3.”
17. Chien WT, Liang CF, (Yu CC), Lin CH, Li SP, Primadona I, Chen YJ, Mong KKT, Lin CC* (2014-04) Chemical Communications 50(43), 5786–5789 “Sequential One-Pot Enzymatic Synthesis of Oligo-N-Acetyllactosamine and its Multi-Sialylated Extensions.”
18. Wang HC, (Yu CC), Liang CF, Huang LD, Hwu JR, Lin CC* (2014-04) ChemBioChem 15(6), 829–835 “Site-Selective Protein Immobilization via 2-Cyanobenzothiazole-Cysteine Condensation.”
19. (Yu CC), Hill T, Kwan DH, Chen HM, Lin CC, Wakarchuk W, Withers SG* (2014-01) Analytical Biochemistry 444, 67–74 “A Plate Based High-Throughput Activity Assay for Polysialyltransferase from Neisseria meningitides.”
20. Adak AK, (Yu CC), Liang CF, Lin CC* (2013-12) Current Opinion in Chemistry Biology 17(6), 1030–1038 “Synthesis of Sialic Acid-Containing Saccharides.”
21. (Yu CC), Huang LD, Kwan D, Wakarchuk WW, Withers SG*, Lin CC* (2013-09) Chemical Communications 49(86), 10166–10168 “A Glyco-Gold Nanoparticle Based Assay for α-2,8-Polysialyltransferase from Neisseria meningitides.”
22. Lin PC*, (Yu CC), Wu HT, Lu YW, Han CL, Su AK, Chen YJ*, Lin CC* (2013-01) Biomacromolecules 14(1), 160–168 “A Chemically Functionalized Magnetic Nanoplatform for Rapid and Specific Biomolecular Recognition and Separation.”
23. (Yu CC), Kuo YY, Liang CF, Chien WT, Wu HT, Chang TC, Jan FD, Lin CC* (2012-04) Bioconjugate Chemistry 23(4), 714–723 “Site-Specific Immobilization of Enzymes on Magnetic Nanoparticles and their Use in Organic Synthesis.”
24. Chien WT, Liang CF, (Yu CC), Lin JH, Wu HT, Lin CC* (2012-01) Advanced Synthesis & Catalysis 354(1), 123–132 “Glucose-1-phosphate Thymidylyltransferase in the Synthesis of UDP-Galactose and its Application in the Synthesis of LacNAc.”
25. Wei-Ting Chien, (Yu CC), Liang CF, Lai CH, Lin PC, Lin CC* (2011) Petite and Sweet: Glyco-Nanotechnology as a Bridge to New Medicines, ACS Symposium Series, Volume 1091, Chapter 2, 15–36 “Functionalized Glyconanoparticles for the Study of Glycobiology.” Huang X. Ed., American Chemical Society
26. Hsiao HY, Chen ML, Wu HT, Huang LD, Chien WT, (Yu CC), Jan FD, Sahabuddin S, Chang TC, Lin CC* (2011-01) Chemical Communications 47(4), 1187–1189 “Fabrication of Carbohydrate Microarrays through Boronate Formation.”
27. (Yu CC), Lin PC, Lin CC* (2008-01) Chemical Communications (11), 1308–1310 “Site-Specific Immobilization of CMP-Sialic Acid Synthetase on Magnetic Nanoparticles and its Use in the Synthesis of CMP-Sialic Acid.”
28. Lin PC, Ueng SH, Yu SC, Jan MD, Adak AK, (Yu CC), Lin CC* (2007-05) Organic Letters 9(11), 2131–2134 “Surface Modification of Magnetic Nanoparticle via Cu(I)-Catalyzed Alkyne-Azide [2 + 3] Cycloaddition.”