Proteomics Mass Spectrometry Common Facility
Staff Scientist：Yi-Yun Chen
Ming-Chieh Tsai, Ronald Lin
Technical Director：Kay-Hooi Khoo
The centralized common mass spectrometry facilities located on the 4F at IBC includes a fleet of hybrid and Tribrid Orbitrap instruments configured primarily for advanced nanoLC-nanoESI-MS/MS analyses. At present, the Thermo LTQ-Orbitrap Velos and Elite systems are both used for routine proteomic ID and first screen analyses of less complex samples, while the high end Orbitrap Fusion and Lumos systems are the workhorses for advanced quantitative shotgun proteomics, PTMomics, glycoproteomics and glycomics. These are administered and operated under the Academia Sinica Common Mass Spectrometry Facilities for protein modification and proteomic analyses, the details of which including different modes of service and instrument bookings, and their respective charges can be found at https://sites.google.com/view/ascmsf/. The IBC facilities further house a Waters Synapt G2 HDMS configured with a fully automated hydrogen-deuterium exchange (HDX) system for protein structure and conformation analysis. Additional peptide and glycan mapping and sequencing are supported by a Sciex MALDI-TOF/TOF 5800 system. Interested new users are strongly advised to first contact the facility Manager and/or Technical Director to discuss research needs and workflow design from front end sample preparation to data analysis. Training courses, seminars and lectures are also on offer for potential users.