Mammalian cell-based recombinant protein expression
User Committee Members
Takashi Angata、Hung-Yuan (Peter) Chi、Chung-I Chang (Chair)、Guang-Chao Chen、Chin-Chun Hung、Meng-Chiao Ho、Chun-Hung Lin、Hsiao-Ching Lin、Steven Lin
Location
Rm812
Contact
Ying-Chu Lee
ycl@gate.sinica.edu.tw
(02)27855696 #8160
Introduction
Mammalian cell-based recombinant protein expression facility provides a sterile environment and instruments (Biosafety Cabinet, Carbon Dioxide Incubators, Cell Counters, Water bath, Temperature-Controlled Centrifuge and microscopes) of cell culture for scientists in gene transfection and protein production. The facility specializes in mammalian and insect cells expression systems. These systems supply the expression and modification requirements for a wide variety of proteins.
1. Thermo Scientific™ Forma™ Steri-Cycle™ CO2 Incubators
Type: Thermo 370
please contact manager
Thermo 370 CO2 Incubators which offer high-temperature decontamination cycle and a unique in-chamber HEPA air filtration system. This incubator is use for culturing with 35mm, 100mm dishes or 25T, 75T flasks.
2. Thermo Scientific Large Capacity CO2 Incubator
Type: Thermo 3950
please contact manager
Thermo 3950 Incubators, equipped with two shakers, is use for large scale protein expression in mammalian cells. Two incubation condition can be selected, one is 37℃, 8% CO2 and the other is 32℃, 8% CO2.
3. Thermo Scientific™ MaxQ™ 6000 Incubated/Refrigerated Stackable Shakers
Type: Thermo MaxQ6000
please contact manager
Thermo MaxQ6000 Incubators is use for protein expression in insect cells.
4. Class II, Type A2 Biological Safety Cabinet
Type: Thermo 1377
please contact manager
These safety cabinets, in accordance with NSF/ANSI 49, offer operator and environment protection.
5. Countess™ Automated Cell Counter
Type: C10227
please contact manager
The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.
6. Fluorescence Inverted Microscope
Type: Leica DM IL LED Fluorescence Inverted Microscope
please contact manager
The Leica DM IL LED Fluorescence Inverted Microscope is ideal for cell culture and routine live cell examinations.
1. Available for 4X, 10x, 20x, and 40x magnification.
2. High-intensity, high-contrast 5W LED illumination.
3. Equipped with a 3 position fluorescence slider (405, FITC, TRITC).
4.CANON camera.
7. Centrifuge
Type: Beckman Coulter J6-MI
please contact manager
The J6-MI centrifuge works with JS-4.2A rotor, max speed is 4200 RPM. It is used for separation of protein production and cells.