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CryoEM studies of pre-mRNA splicing reveal assembly and activation of the spliceosome

2018-02-27 @ 11:00:00 - 12:00:00

  • Time Y/M/D: 2018/2/27 11:00 ~ 12:00
  • Venue: IBC R114
  • Speaker: Dr. Pei-Chun Lin
  • Current Position: Investigator Scientist, MRC Laboratory of Molecular Biology, Cambridge, UK
  • Speaker Website:Website
  • Host: Academician Ming-Daw Tsai
  • Website:Link
  • Contact: Ms. Nancy Liu
  • Tel: 02-27855696#1165
  • E-mail: liukchun@gate.sinica.edu.tw

Intron removal requires recognition of exon-intron boundaries, stepwise assembly of the spliceosome from five small nuclear ribonucleoprotein particles (snRNPs; U1, U2, U4, U5, U6) and extensive remodelling to form the spliceosome's catalytic center. In the beginning of pre-mRNA splicing, the intron 5'-splice site (5'SS) and the branch point (BP) sequence are selected and brought into the prespliceosome by the U1 and U2 snRNPs. The U4/U6.U5 tri-snRNP subsequently joins the prespliceosome to form the complete pre-catalytic spliceosome, which later converts to the catalytic active spliceosome. In my talk, I will report cryo-electron microscopy structures of the assembling yeast spliceosome. The data reveal important regulatory splicing intermediates and provide crucial insights into the mechanism of spliceosome activation.-Dr. Pei-Chun Lin

seminar poster


11:00:00 - 12:00:00


IBC R114