Hsu 20200103 papers

Through the looking glass of cryoEM, unveiling the unique molecular structure and camouflaging sugar coating of feline coronavirus surface spike protein

Yang TJ, Chang YC, Ko TP, Draczkowski P, Chien YC, Chang YC, Wu KP, Khoo KH, Chang HW*, Hsu STD*
The recent outbreaks of severe acute respiratory syndrome (SARS), the Middle East respiratory syndrome (MERS), and the severe pig pandemics caused by the emerging porcine coronavirus, remind us how infection by coronaviruses can impact our health and socioeconomics.

Lin ch 20191016

The enzymatic tools that catalyze a stepwise demethylation process on terpene scaffold

Chi-Fang Lee, Li-Xun Chen, Chen-Yu Chiang, Chen-Yu Lai, Hsiao-Ching Lin*
A research team led by Dr. Hsiao-Ching Lin, an assistant research fellow at the Institute of Biological Chemistry, has elucidated and characterized the biosynthetic pathway of the aculenes, a unique class of fungal norsequiterpenes.

ChenRH 2019 ASB11s Photo

Cell biology studies identify the key control of cell life-death decision for the development of a new anti-cancer strategy

Chen FY, Huang MY, Lin YM, Ho CH, Lin SY, Chen HY, Hung MC, Chen RH
A research team led by Dr. Ruey-Hwa Chen, Distinguished Research Fellow at the Institute of Biological Chemistry, has discovered a ubiquitin-dependent mechanism in controlling the life-death cell fate in response to different cellular stresses.

Yao 2019 Pub small

Uncovering the mystery of how oxidative stress, the culprit of aging and neurodegenerative diseases, accumulates and operates in the motor system

Peng JJ, Lin SH, Liu YT, Lin HC, Li TN, Yao CK
Increased oxidative stress is considered to be one of the key factors leading to aging and neurodegenerative diseases, but it is not clear why oxidative stress can accumulate in the nervous system and affect the functioning of the nervous system.

tsai 20192s

IBC Researchers Discovered That Human DNA Polymerase μ Can Use a Noncanonical Mechanism for Multiple Mn2+-mediated Functions

Chang YK, Huang YP, Liu XX, Ko TP, Bessho Y, Kawano Y, Maestre-Reyna M, Wu WJ*, Tsai MD*
A team led by Academician Ming-Daw Tsai, a Distinguished Research Fellow, and Dr. Wen-Jin (Winston) Wu, an Associate Research Specialist at the Institute of Biological Chemistry has discovered that human DNA polymerase μ can use a noncanonical mechanism for multiple Mn2+-mediated functions. The research article has appeared on the May 29th issue of Journal of the American Chemical Society.

Coverfigure 001

Sharp Tools Make Good Work: The Early Success of ASCEM

Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
Dr. Ming-Daw Tsai, Director of Academia Sinica Cryo-EM Center (ASCEM) and Distinguished Research Fellow of Institute of Biological Chemistry, collaborates with Dr. Chin-Yu Chen, an assistant professor from Department of Life Science National Central University, in using the state of the art technology, cryo-electron microscopy (cryo-EM), to uncover the structural bases of pH effect and cofactor bispecificity of ketol-acid reductoisomerase from archaea Sulfolobus solfataricus. The result is published in the Journal of the American Chemical Society on April 17 and featured on the Journal cover.

Ho MC 20191 s

Rice submergence resistance mechanism

Lin CC, Chao YT, Chen WC, Ho HY, Chou MY, Li YR, Wu YL, Yang HA, Hsieh H, Lin CS, Wu FH, Chou SJ, Jen HC, Huang YH, Irene D, Wu WJ, Wu JL, Gibbs DJ, Ho MC, Shih MC
Rice can be drowned! Although rice is a semi-aquatic plant, the seedlings will die if they are submerged completely under water and oxygen is depleted. Recent climate change has transformed once-in-a-lifetime typhoon or heavy rainfall into a frequent occurrence, which greatly reduced rice production in Taiwan.

wang 20183 s

The C-terminal D/E-rich domain of MBD3 is a putative Z-DNA mimic that competes for Zα DNA-binding activity

Lee CH, Shih YP, Ho MR, Wang AH*
Academician Andrew H.-J. Wang and his team at the Institute of Biological Chemistry have recently found that the C-terminal D/E-rich domain of MBD3 was able to associate with the Z-DNA binding domain (Zα) of ADAR1. The D/E-rich domain of MBD3 may act as a DNA mimic to compete with Z-DNA for binding to Zα. The interplay of MBD3 and ADAR1 may participate in regulating the local DNA conformational transition between B-DNA and Z-DNA and thereby results in alterations in gene expression.

wu 20182 s

Pseudaminic acid on exopolysaccharide of Acinetobacter baumannii plays a critical role in phage-assisted preparation of glycoconjugate vaccine with high antigenicity

I-Ming Lee, Feng-Ling Yang, Te-Li, Chen, Kuo-Shiang Liao, Chien-Tai Ren, Nien-Tsung Lin, Yu-Pei Chang, Chung-Yi Wu* and Shih-Hsiung Wu*
Infection by antibiotic resistant bacterial pathogens poses a serious global health threat. Therefore, a new therapy is urgently required to overcome the failure of antibiotic. Glycoconjugate vaccine have been proven to be effective in combating a broad spectrum of diseases caused by antibiotic resistant bacteria, and was suggested as a promising solution. In this regard, rising the efficacy of glycoonjugate preparation and biological activity of glycoconjugate-induced antibody will accelerate the development of vaccination. The research team lead by Dr. Shih-Hsiung Wu, a Distinguished Research Fellow in the institute of Biological Chemistry, and Dr. Chung-Yi Wu, a Research Fellow in the Genomic Research Center found that pseudaminic acid (Pse) plays a critical role on this field and these results had published in Journal of the American Chemical Society on July 2nd, 2018.

wu 2018 s

Solving the acting target and molecular mechanism of Z-(+)-isochaihulactone

Jeffy Chern, Chun-Ping Lu, Zhanxiong Fang, Ching-Ming Chang, Kuo-Feng Hua, Yi-Ting Chen, Cheng Yang Ng, Yi-Lin Sophia Chen, Yulin Lam,* and Shih-Hsiung Wu*
Traditional medicines provide a fertile ground to explore potent “lead” compounds with desired effects; yet, their transformation into modern drugs is often fraught with challenges in deciphering the target that is mechanistically valid for its biological activity. The research team led by Dr. Shih-Hsiung Wu, a Distinguished Research Fellow in the Institute of Biological Chemistry, and collaborators in National Ilan University and National University of Singapore determined the acting target and molecular mechanism of Z-(+)-isochaihulactone (active lignin from a traditional Chinese medicine – Nan Chai Hu, Bupleurum scorzonerifolium) and whether the compound can serve as a promising lead compound for future drug development. The paper has been published in Angewandte Chemie International Edition on June 11, 2018.