20220823 Highlight s1

Cryo-electron tomography reveals the structure and dynamics of an alphacoronavirus spike protein

Huang CY, Draczkowski P, Wang YS, Chang CY, Chien YC, Cheng YH, Wu YM, Wang CH, Chang YC, Chang YC, Yang TJ, Tsai YX, Khoo KH, Chang HW, Hsu STD*
Porcine epidemic diarrhea virus (PEDV) belongs to the alphacoronavirus family. PEDV is highly contagious and particularly lethal for infected piglets. Despite its apparent threat to the pork industry, Taiwan has no effective vaccination program.

20220519 Highlight s 1

Molecular insights of how oncogene suppressor BAP1’s nuclear import is regulated by TNPO1

Yang TJ, Li TN, Huang RS, Pan MY, Lin SY, Lin S, Wu KP, Wang LH, Hsu STD*
Dr. Shang-Te Danny Hsu at the Institute of Biological Chemistry reported the molecular basis of the nuclear import of an important oncogene suppressor BAP1, which is governed by an atypical nuclear import factor called TNPO1. TNPO1 binding to the atypical nuclear localization signal (NLS) of BAP1, known as the PY-motif, prevents the competing action of an atypical E2/E3 conjugating enzyme, UBE2O, which favors cytosolic retention of BAP1. This work, which is published in the Journal of Cell Biology, substantially expands our fundamental understanding of BAP1 cancer biology.

20220307 highlights s

A cell-free self-replenishing CO2-fixing system

Luo S, Lin PP, Nieh LY, Liao GB, Tang PW, Chen C, Liao JC*
Biological CO2 fixation is so far the most effective means for CO2 reduction at scale and accounts for most of the CO2 fixed on Earth. Through this process, carbon is fixed in cellular components and biomass during organismal growth. To uncouple CO2 fixation from growth and cellular regulation

Hsu 20210916

Molecular insights into the increased transmissibility and immunity evasion of the SARS-CoV-2 Alpha variant

Yang TJ, Yu PY, Chang YC, Liang KH, Tso HC, Ho MR, Chen WY, Lin HT, Wu HC, Hsu STD*
The Alpha variant of SARS-CoV-2, also known as the B.1.1.7 strain, was first identified in the UK in late 2020. It soon spread across the world before being overtaken by the Delta variant that emerged in India. These variants of concern (VOCs) are essentially defined by the mutations on the spike protein.

20210326 highlights s

Identification of a novel autophagy regulatory mechanism for combating fatty liver

Chen YH, Huang TY, Lin YT, Lin SY, Li WH, Hsiao HJ, Yan RL, Tang HW, Shen ZQ, Chen GC, Wu KP, Tsai TF, Chen RH*

Fatty liver is a prevalent liver disease in Taiwan and worldwide and is associated with obesity, diabetes and cardiovascular diseases. Although fatty liver is normally asymptomatic, its progression can lead to hepatitis, cirrhosis, and even liver cancer.

20210219 highlights s 1

Protein instability links to intellectual disability – a breakthrough in the cerebral development

Chiang SY, Wu HC, Lin SY, Chen HY, Wang CF, Yeh NH, Shih JH, Huang YS, Kuo HC, Chou SJ*, Chen RH*
Cerebral cortex is responsible for sensory, motor, emotional processing and higher cognitive functions. The development of cerebral cortex is a complex process involving several steps, such as neurogenesis, neuron migration, cortical organization and circuit formation.

20210129 highlights s

Perfection in the imperfection – molecular mechanism for genetic diversity

Luo SC, Yeh HY, Lan WH, Wu YM, Yang CH, Chang HY, Su GC, Lee CY, Wu WJ, Li HW, Ho MC*, Chi P*, Tsai MD*
Genetic diversity is a key driving force of evolution. During homologous recombination, chromosomes from father and mother will pair together to exchange the genetic material leading to genetic diversity. In the process of chromosome pairing, the DMC1 protein is the key enzyme that catalyzes the DNA exchange reaction.

20201210 Yao CK s

A positive feedback loop between Flower and PI(4,5)P 2 at periactive zones controls bulk endocytosis in Drosophila

Tsai-Ning Li, Yu-Jung Chen, You-Tung Wang, Hsin-Chieh Lin, Ting-Yi Lu, Chi-Kuang Yao*
Synaptic vesicle (SV) endocytosis is coupled to exocytosis to maintain SV pool size and thus neurotransmitter release. Intense stimulation induces activity-dependent bulk endocytosis (ADBE) to recapture large quantities of

20201119 Chi P s

Human CST complex protects stalled replication forks by directly blocking MRE11 degradation of nascent-strand DNA

Xinxing Lyu, Kai‐Hang Lei, Pau Biak Sang, Olga Shiva, Megan Chastain, Peter Chi*, Weihang Chai*
Degradation and collapse of stalled replication forks are main sources of genomic instability, yet the molecular mechanisms for protecting forks from degradation/collapse are not well understood. Here, we report that human CST (CTC1-STN1-TEN1) proteins, which form a single-stranded DNA-binding complex, localize at stalled forks and protect stalled forks from degradation by the MRE11 nuclease.

20201201 highlights s

Cross-species detection and bactericidal activity for pseudaminic acid-coated bacteria

I-Ming Lee, I-Fan Tu, Feng-Ling Yang and Shih-Hsiung Wu*
Pseudaminic acid (Pse), a unique carbohydrate in surface-associated glycans of pathogenic bacteria, has pivotal roles in virulence. Therefore, the detection and therapy targeted on Pse turn into an attractive issue. We reported here that a catalytically inactive ΦAB6TSP (I-ΦAB6TSP) retains binding ability toward Pse