中央研究院 生物化學研究所

研究領域與專長

Tristetraprolin（TTP）的家族包含三個主要成員，TTP、ZFP36L1和ZFP36L2。它們具有高相似性的RNA結合區，可以與多AU序列的訊息核糖核酸結合來降低訊息RNA的穩定性，我們的研究顯示三個TTP家族成員經由調控特別的目標RNA而參與巨噬細胞的活化和脂肪細胞的分化。訊息RNA降解的第一個步驟是去除3’端的腺苷酸，由PAN2-PAN3和CCR4-NOT這兩類去腺苷酸酶複合體負責；已知TTP可與複合體中的CNOT1結合而導致目標RNA的去腺苷酸和不穩定，我們想進一步探究不同的TTP家族成員如何與CCR4和CAF1這兩種去腺苷酸酶的旁系蛋白作用，而此兩種去腺苷酸酶在複合體中又是如何協調合作，還有TTP 家族蛋白的磷酸化如何影響與腺苷酸酶複合體的結合作用。

學經歷

• 1990 – 1994   博士, 生化科學所, 臺灣大學
• 1987 – 1989   碩士, 生化科學所, 國立臺灣大學
• 1983 – 1987   學士, 農業化學系, 國立臺灣大學

• 2002 – 迄今   副研究員, 中研院生化所
• 1994 – 2002   助研究員, 中研院生化所
• 1989 – 1994   助理研究員, 中研院生化所

主要著作

Phosphorylation of mRNA Decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes.  
Chiang, P.Y., Shen, Y.F., Kao, C.H., Su, Y.L., Lin, N.Y., Hsu, P.H., Tsai, M.D., Wang, S.C., Chang, G.D., Lee, S.C., and Chang, C.J.  
PLos One (2013)

Reversible acetylation regulates Salt-inducible kinase (SIK2) and its function in autophage.  
Yang, F.C., Tan, B., Chen, W.H., Lin, Y.H., Huang, J.Y., Chang, H.Y., Sun, H.Y., Hsu, P.H., Liou, G.G., Shen, J., Chang, C.J., Han, C.C., Tsai, M.D., and Lee, S.C.  
Journal of Biological Chemistry (2013)

Transcriptional regulation of tristetraprolin by NF-κB signaling in LPS-stimulated macrophages.  
Chen, Y.L., Jiang, Y.W., Su, Y.L., Lee, S.C., Chang, M.S., and Chang C.J.  
Molecular biology reports (2013)

Differential expression and functional analysis of the tristetraprolin family during early differentiation of 3T3-L1 preadipocytes.  
Lin, N.Y., Lin, T.Y., Yang, W.H., Wang, S.C., Wang, K.T., Su, Y.L., Jiang, Y.W., Chang, G.D., and Chang, C.J.  
International journal of biological sciences (2012)

Drosophila eyes absent is a Novel mRNA Target of the Tristetraprolin (TTP) Protein DTIS11.  
Yeh, P.A., Yang, W.H., Chiang, P.Y., Wang, S.C., Chang, M.S. and Chang, C.J.  
International journal of biological sciences (2012)

The Role of Nocturnin in Early Adipogenesis and Modulation of Systemic Insulin Resistance in Human.  
Hee, S.W., Tsai, S.H., Chang, Y.C., Chang, C.J., Yu, I.S., Lee, P.C., Lee, W.J., Yun-Chia Chang, E. and Chuang, L.M.  
Obesity (Silver Spring) (2012)

Tristetraprolin inhibits poly(A)-tail synthesis in nuclear mRNA that contains AU-rich elements by interacting with poly(A)-binding protein nuclear 1.  
Su, Y.L., Wang, S.C., Chiang, P.Y., Lin, N.Y., Shen, Y.F., Chang, G.D., and Chang C.J.  
PLoS One (2012)

Modulation of immediate early gene expression by tristetraprolin in the differentiation of 3T3-L1 cells.  
Lin, N.Y., Lin, C.T. and Chang, C.J.  
Biochemical and biophysical research communications (2008)

Regulation of tristetraprolin during differentiation of 3T3-L1 preadipocytes.  
Lin, N.Y., Lin, C.T., Chen, Y.L. and Chang, C.J.  
The FEBS journal (2007)

Differential regulation of ARE-mediated TNFalpha and IL-1beta mRNA stability by lipopolysaccharide in RAW264.7 cells.  
Chen, Y.L., Huang, Y.L., Lin, N.Y., Chen, H.C., Chiu, W.C. and Chang, C.J.  
Biochemical and biophysical research communications (2006)

著作列表

1. Chang YJ, Kang Z, Bei J, Chou SJ, Lu MJ, Su YL, Lin SW, Wang HH, Lin S*, (Chang CJ)*  (2022-06)  International journal of molecular sciences  23(12), 6839  "Generation of TRIM28 Knockout K562 Cells by CRISPR/Cas9 Genome Editing and Characterization of TRIM28-Regulated Gene Expression in Cell Proliferation and Hemoglobin Beta Subunits."
   
2. Hsieh HH, Chen YA, Chang YJ, Wang HH, Yu YH, Lin SW, Huang YJ, Lin S, (Chang CJ)*  (2021-06)  Journal of Inflammation-London  18(1), 22  "The functional characterization of phosphorylation of tristetraprolin at C-terminal NOT1-binding domain."
   
3. Lu HP, Lin CJ, Chen WC, Chang YJ, Lin SW, Wang HH, (Chang CJ)  (2020-09)  Int. J. Mol. Sci.  21(19), E7245  "TRIM28 Regulates Dlk1 Expression in Adipogenesis"
   
4. Yeh CC, (Chang CJ), Twu YC, Chu CC, Liu BS, Huang JT, Hung ST, Chan YS, Tsai YJ, Lin SW, Lin M, Yu LC  (2018-08)  Blood advances  2(15), 1854-1864  "The molecular genetic background leading to the formation of the human erythroid-specific Xg(a)/CD99 blood groups."
   
5. Yeh CC, (Chang CJ), Twu YC, Hung ST, Tsai YJ, Liao JC, Huang JT, Kao YH, Lin SW, Yu LC  (2018-04)  Transfusion  58(4), 1054-1064  "The differential expression of the blood group P(1) -A4GALT and P(2) -A4GALT alleles is stimulated by the transcription factor early growth response 1."
   
6. Yeh PA, (Chang CJ)  (2017)  PLOS ONE  12(10), e0186037  "A novel function of twins, B subunit of protein phosphatase 2A, in regulating actin polymerization."
   
7. Wang HH, Lin CY, Su SH, Chuang CT, Chang YL, Lee TY, Lee SC, (Chang CJ)  (2016)  Cell Death and Disease  7(7), e2298  "Activation of salt-inducible kinase 2 promotes the viability of peritoneal mesothelial cells exposed to stress of peritoneal dialysis."
   
8. Wang KT, Wang HH, Wu YY, Su YL, Chiang PY, Lin NY, Wang SC, Chang GD, (Chang CJ)*   (2015-07)  Journal of Inflammation-London  12, 42  " Functional regulation of Zfp36l1 and Zfp36l2 in response to lipopolysaccharide in mouse RAW264.7 macrophages."
   
9. Chiang PY, Shen YF, Su YL, Kao CH, Lin NY, Hsu PH, Tsai MD, Wang SC, Chang GD, Lee SC, (Chang CJ)  (2013-04)  PloS one  8(4), e61697  "Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes."
   
10. Yu-Ling Chen, Yu-Wun Jiang, Yu-Lun Su, Sheng-Chung Lee, Mau-Sun Chang and (Ching-Jin Chang)*  (2013-04)  MOLECULAR BIOLOGY REPORTS  40(4), 2867-2877  "Transcriptional Regulation of Tristetraprolin by NF-κB Signaling in LPS-stimulated Macrophages"
    
11. Yang FC, Tan BC, Chen WH, Lin YH, Huang JY, Chang HY, Sun HY, Hsu PH, Liou GG, Shen J, (Chang CJ), Han CC, Tsai MD, Lee SC  (2013)  The Journal of biological chemistry  288(9), 6227-6237  "Reversible acetylation regulates salt-inducible kinase (SIK2) and its function in autophagy."
    
12. Hu CK, Lee YJ, Colitz CM, (Chang CJ), Lin CT*  (2012-09)  VETERINARY OPHTHALMOLOGY  15(s2), 65-71  "The protective effects of Lycium barbarum and Chrysanthemum morifolum on diabetic retinopathies in rats"
    
13. Hee SW, Tsai SH, Chang YC, (Chang CJ), Yu IS, Lee PC, Lee WJ, Yun-Chia Chang E, Chuang LM*  (2012-08)  OBESITY  20(8), 1558-1565  "The role of nocturnin in early adipogenesis and modulation of systemic insulin resistance in human"
    
14. Yu-Lun Su, Shun-Chang Wang, Pei-Yu Chiang, Nien-Yi Lin, Yu-Fang Shen, Geen-Dong Chang, and (Ching-Jin Chang*)  (2012-07)  PLoS One  7(7), e41313  "Tristetraprolin Inhibits Poly(A)-Tail Synthesis in Nuclear mRNA That Contains AU-rich Elements by Interacting with Poly(A)-Binding Protein Nuclear 1 "
    
15. Lin NY, Lin TY, Yang WH, Wang SC, Wang KT, Su YL, Jiang YW, Chang GD, (Chang CJ*).  (2012-05)  International Journal of Biological Sciences  8(5), 761-777  "Differential Expression and Functional Analysis of the Tristetraprolin Family during Early Differentiation of 3T3-L1 Preadipocytes."
    
16. Yeh PA, Yang WH, Chiang PY, Wang SC, Chang MS, (Chang CJ*)  (2012-04)  International Journal of Biological Sciences  8(5), 606-619  "Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11"
    
17. Yeh PA, (Chang CJ), Tu PH, Wilson HI, Chien JY, Tang CY, Su MT  (2010)  Journal of Alzheimer's disease : JAD  21(2), 543-556  "Phosphorylation alters tau distribution and elongates life span in Drosophila."
    
18. Nien-Yi Lin, Tzi-Yang Lin, Ching-Jin Chang, and Chung-Tien Lin  (2009-09)  Taiwan Veterinary Journal.  35(3), 174-184  "Analysis of ARE-binding proteins associated with 3’ untranslated region of MKP-1 mRNA"
    
19. N.-Y. Lin, C.-T. Lin, and C.-J. Chang  (2008)  Biochemical and Biophysical Research Communications  365, 69-74  "Modulation of immediate early gene expression by tristetraprolin in the differentiation of 3T3-L1 cells"
    
20. Chiung-Wen Chang, Han-Yi Chou, Yu-Sheng Lin, Kuo-Hsiang Huang, (Ching-Jin Chang), Tsui-Chun Hsu and Sheng-Chung Lee  (2008)  BMC Molecular Biology  9, 61  "Phosphorylation at Ser473 regulates heterochromatin protein 1 binding and corepressor function of TIF1beta/KAP1."
    
21. N.-Y. Lin, C.-T. Lin, Y.-L. Chen and C.-J. Chang  (2007)  FEBS J  274, 867-878  "Regulation of tristetraprolin during differentiation of 3T3-L1 preadipocytes"
    
22. Y.L. Chen, Y.L. Huang, N.Y. Lin, H.C. Chen and C.J. Chang  (2006)  Biochemical and Biophysical Research Communications  346(1), 160-168  "Differential regulation of ARE-mediated TNFalpha and IL-1beta mRNA stability by lipopolysaccharide in RAW264.7 cells."
    
23. W.Y. Yang, C.H. Liu, C.J. Chang, L.C. Lee, K.J. Chang and C.T. Lin  (2006)  Journal of Comparative Pathology  134, 70-79  "Proliferative activity, apoptosis and expression of oestrogen receptor and Bcl-2 oncoprotein in canine mammary gland tumours"
    
24. J.L. Lee, C.J. Chang, L.L. Chueh and C.T. Lin  (2006)  Breast Cancer Research and Treatment  100, 49-58  "Secreted Frizzled Related Protein 2 (sFRP2) Decreases Susceptibility to UV-Induced Apoptosis in Primary Culture of Canine Mammary Gland Tumors by NF-kappaB Activation or JNK Suppression"
    
25. J. L. Lee, C. T. Lin, L. L. Chueh and C. J. Chang  (2004)  J Biol Chem  279, 14602-14609  "Autocrine/paracrine SFRP2 induces cellular resistance to apoptosis: A possible mechanism of mammary tumorigenesis"
    
26. Lee JL, Chang CJ, Wu SY, Sargan DR, Lin CT  (2004)  Breast cancer research and treatment  84(2), 139-149  "Secreted frizzled-related protein 2 (SFRP2) is highly expressed in canine mammary gland tumors but not in normal mammary glands."
    
27. Su WC, Chou HY, Chang CJ, Lee YM, Chen WH, Huang KH, Lee MY, Lee SC  (2003)  The Journal of biological chemistry  278(51), 51150-51158  "Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene."
    
28. J. L. Lee, C. J. Chang, L. L. Chueh and C. T. Lin  (2003)  In Vitro Cellular & Developmental Biology-Animal  39(5-6), 221-227  "Expression of secreted frizzled-related protein 2 in a primary canine mammary tumor cell line: a candidate tumor marker for mammary tumor cells"
    
29. H. C. Chen, W. C. Lin, Y. G. Tsay, S. C. Lee and C. J. Chang*  (2002)  J Biol Chem  277, 40403-40409  "An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K"
    
30. C. M. Chiu, Y. G. Tsay, C. J. Chang and S. C. Lee  (2002)  J Biol Chem  277, 39102-39111  "Nopp140 is a mediator of the protein kinase A signaling pathway that activates the acute phase response alpha1-acid glycoprotein gene"
    
31. W. C. Lin, B. J. Shen, Y. G. Tsay, H. C. Yen, S. C. Lee and C. J. Chang  (2002)  DNA Cell Biol  21, 551-560  "Transcriptional activation of C/EBPbeta gene by c-Jun and ATF2"
    
32. C. J. Chang, H. W. Luh, S. H. Wang, H. J. Lin, S. C. Lee and S. T. Hu  (2001)  DNA Cell Biol  20, 569-577  "The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with dengue virus core protein"
    
33. C. J. Chang, Y. L. Chen and S. C. Lee  (1998)  Mol Cell Biol  18, 5880-5887  "Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression"
    
34. L. H. Miau, C. J. Chang, B. J. Shen, W. H. Tsai and S. C. Lee  (1998)  J Biol Chem  273, 10784-10791  "Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation"
    
35. L. H. Miau, C. J. Chang, W. H. Tsai and S. C. Lee  (1997)  Mol Cell Biol  17, 230-239  "Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a transcription factor"
    
36. B. J. Shen, C. J. Chang, H. S. Lee, W. H. Tsai, L. H. Miau and S. C. Lee  (1997)  DNA Cell Biol  16, 703-711  "Transcriptional induction of the agp/ebp (c/ebp beta) gene by hepatocyte growth factor"
    
37. Lee YM, Miau LH, Chang CJ, Lee SC  (1996)  Molecular and cellular biology  16(8), 4257-4263  "Transcriptional induction of the alpha-1 acid glycoprotein (AGP) gene by synergistic interaction of two alternative activator forms of AGP/enhancer-binding protein (C/EBP beta) and NF-kappaB or Nopp140."
    
38. C. J. Chang, B. J. Shen and S. C. Lee  (1995)  DNA Cell Biol  14, 529-537  "Autoregulated induction of the acute-phase response transcription factor gene, agp/ebp"
    
39. Chang CJ, Lai MY, Chen DS, Lee SC  (1992)  DNA and cell biology  11(4), 315-320  "Structure and expression of mouse alpha 1-acid glycoprotein gene-3 (AGP-3)."
    
40. C. J. Chang, T. T. Chen, H. Y. Lei, D. S. Chen and S. C. Lee  (1990)  Mol Cell Biol  10, 6642-6653  "Molecular cloning of a transcription factor, AGP/EBP, that belongs to members of the C/EBP family"
    
41. S. C. Lee, C. J. Chang, Y. M. Lee, H. Y. Lei, M. Y. Lai and D. S. Chen  (1989)  DNA  8, 245-251  "Molecular cloning of cDNAs corresponding to two genes of alpha 1-acid glycoprotein and characterization of two alleles of AGP-1 in the mouse"