中央研究院 生物化學研究所

研究領域與專長

The biophysics of Cell-environment interactions: a structural and computational perspective.

While cells in multicellular organisms are presented with fairly constant environments, microbes and protozoans need to react to highly variable conditions. Accordingly, in single-celled organisms, sophisticated systems are localized both at the cellular surface, and in the intracellular sub-compartments, which allow them to quickly and efficiently adjust cell chemistry and gene expression. In my research, I have focused on microbial adhesins, glycan binding proteins which recognize biological surfaces, both in the context of bacterial communities, and infection biology. Further, microbial responses to light, be it in optogenetic systems, or in DNA photodamage repair, has recently become a central area of study for me.

Both of these fields are approached from a biophysical perspective, making use of protein-crystallographic techniques, as well as in-vitro analyses. In this respect, the development of surface modulating additives has been invaluable for my research. Finally, I make use of molecular dynamics simulations and small angle X-ray scattering to highlight protein flexibility and dynamics in a way which mostly escapes crystallographic techniques.

學經歷

• 2011   Dr. Rer. Nat., Philipps University Marburg, Germany.
• 2006   Diploma in Biochemistry, Philipps University Marburg, Germany.
• 2005   Licenciatura degree in Biochemistry, Universidad de Valencia, Spain.
• 2001   First cycle studies in Pharmacy, Universidad de Valencia, Spain.

• 2023 – 迄今 Joint Appointment Assistant Research Fellow, Institute of Biological Chemistry, Academia Sinica, Taiwan.
• 2016 – 2023   Visiting Scholar, Institute of Biological Chemistry, Academia Sinica, Taiwan.
• 2012 – 2016   Postdoctoral Fellow, Institute of Biological Chemistry, Academia Sinica, Taiwan.
• 2011 – 2012   Laborleiter (lab leader), AG Essen, Department of Chemistry, Philipps University Marburg, Germany.
• 2008 – 2011   Wissenschaftlicher Mitarbeiter (Research Associate), Department of Chemistry, Philipps University Marburg, Germany.
• 2006   Research Assistant, Department of Chemistry, Philipps University Marburg, Germany.

主要著作

Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. 
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T. 
Nucleic Acids Res. (2016)

In situ proteolysis of the Vibrio cholerae matrix protein RbmA promotes biofilm recruitment. 
Smith, DR, Maestre-Reyna, M, Lee, G, Gerard, H, Wang, AH-J, Watnick, PI. 
Proc. Natl. Acad. Sci. U. S. A. (2015)

Crowning Proteins: Modulating the Protein Surface Properties using Crown Ethers. 
Lee, C-C, Maestre-Reyna, M, Hsu, K-C, Wang, H-C, Liu, C-I, Jeng, W-Y, Lin, L-L, Wood, R, Chou, C-C, Yang, J-M, Wang, AH-J. 
Angewandte Chemie International Edition (2014)

Structural Insights into RbmA, a Biofilm Scaffolding Protein of V. Cholerae. 
Maestre-Reyna, M, Wu, W-J, Wang, AH-J. 
PloS One (2013)

Structural basis for promiscuity and specificity during Candida glabrata invasion of host epithelia. 
Maestre-Reyna, M, Diderrich, R, Veelders, M S, Eulenburg, G, Kalugin, V, Bruckner, S, Keller, P, Rupp, S, Moesch, H-U, Essen L-O. 
Proceedings of the National Academy of Sciences of the United States of America (2012)

著作列表