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Targeting Protein Tyrosine Phosphatase PTP-PEST for Therapeutic Intervention in Acute Myocardial Infarction

Targeting Protein Tyrosine Phosphatase PTP-PEST for Therapeutic Intervention in Acute Myocardial Infarction

Cardiovasc Res. 2020 Apr 1;116(5):1032-1046
doi: 10.1093/cvr/cvz165

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Yang CF1,2,3Chen YY3Singh JP3,4,5Hsu SF3Liu YW3Yang CY3,6Chang CW3Chen SN3Shih RH7Hsu SD3,4,6Jou YS7Cheng CF2,8,7Meng TC3,4,6

Abstract

AIMS:

The myocardial ischemia/reperfusion (I/R) injury is almost inevitable since reperfusion is the only established treatment for acutemyocardial infarction (AMI). To date there is no effective strategy available for reducing the I/R injury. Our aim was to elucidate the mechanisms underlying myocardial I/R injury and to develop a new strategy for attenuating the damage it causes.

METHODS AND RESULTS:

Using a mouse model established by ligation of left anterior descending (LAD) artery, we found an increase in activity of protein tyrosine phosphatases (PTPs) in myocardium during I/R. Treating the I/R-mice with a pan-PTP inhibitor phenyl vinyl sulfone (PVS) attenuated I/R damage, suggesting PTP activation to be harmful in I/R. Through analyzing RNAseq data, we showed PTPs being abundantly expressed in mouse myocardium. By exposing primary cardiomyocytes ablated with specific endogenous PTPs by RNAi to hypoxia/reoxygenation (H/R), we found a role that PTP-PEST (PTPN12) plays to promote cell death under H/R stress. Auranofin, a drug being used in clinical practice for treating rheumatoid arthritis, may target PTP-PEST thus suppressing its activity. We elucidated the molecular basis for Auranofin-induced inactivation of PTP-PEST by structural studies, and then examined its effect on myocardial I/R injury. In the mice receiving Auranofin before reperfusion, myocardial PTP activity was suppressed, leading to restored phosphorylation of PTP-PEST substrates, including ErbB-2 that maintains the survival signaling of the heart. In line with the inhibition of PTP-PEST activity, the Auranofin-treated I/R-mice had smaller infarct size and better cardiac function.

CONCLUSIONS:

PTP-PEST contributes to part of the damages resulting from myocardial I/R. The drug Auranofin, potentially acting through the PTP-PEST-ErbB-2 signaling axis, reduces myocardial I/R injury. Based on this finding, Auranofin could be used in the development of new treatments that manage I/R injury in patients with AMI.

TRANSLATIONAL PERSPECTIVE:

Cardiac ischemia/reperfusion (I/R) injury remains a significant health concern worldwide. A novel intervention for attenuating this detrimental effect is an unmet clinical need. Our study elucidates the role that protein tyrosine phosphatasePTP-PEST plays in promoting cardiac I/R injury. Auranofin, which is a FDA-approved drug, can inhibit the activity of PTP-PEST, leading to protection of the heart under I/R stress. These findings suggest the potential that Auranofin is holding to treat patients suffering acutemyocardial infarction.